Quality Control Assurance
Resume:
MICHELLE PIRES
** ******* ******, *** # ** ~ Boston, MA 02115 *****.*@*****.***.***
SUMMARY
Research Assistant experienced with molecular biology and pharmacology techniques like cell culture, ELISA, gene
expression analysis, PCR, western blots, transfection. A self-driven and hard-working individual with excellent
communication and organizational skills and the ability to work independently and efficiently in a research setting. .
EDUCATION
Northeastern University, Boston, MA May 2013
Master of Science in Pharmaceutical Sciences: Pharmacology
GPA: 3.8
Related Courses: Pharmaceutical Sciences Lab, Anatomy Physiology and Pathophysiology, Concepts in Pharmaceutical
Sciences, Molecular Cell Biology, Ethics in Biological Research, Receptor Pharmacology, General Pharmacology, Drug
Design Evaluation & Development, Experimental Design & Biometrics, Biotechnology Applications lab course
The University of Mumbai, Mumbai, India June 2010
Mumbai Educational Trust’s Institute of Pharmacy
Bachelor of Pharmacy
GPA: 4.0
SKILLS
Lab Skills: Experienced with Transfection, Sterile Cell culture techniques: Thawing, Freezing, Counting, Plating, Passaging
and splitting, Western Blot, SDS-PAGE, PCR, Secreted alkaline phosphatase (SEAP) assay, Metridia Luciferase assay,
Binding assays, Immunofluorescence, Bicinchoninic (BCA) Assay, ELISA, Cytotoxicity assay, Affinity chromatography, RNA
extraction, DNA quantification (mini prep), DNA sequencing, Primer design
Familiar with Enzyme kinetics, preparation of gold and gelatin nanoparticles, qRTPCR, HPLC, Mass spectroscopy
Computer: Microsoft Office, NCBI Blast, APE, Gen5, SPSS, Soft Max Pro
Languages: Fluent in English, Hindi, and Marathi
EXPERIENCE
Dr. Ralph Loring’s Laboratory, Northeastern University, Boston, MA May 2012-May 2013
Research Assistant
Project: Studying the anti-inflammatory effects of nicotine by investigating the blockage of the Nfkb signaling pathway by
transfecting the HEK wild type cells with Nfkb driven SEAP reporter plasmid expression of alpha 7 and Ric3 chaperone.
• Transiently transfected HEK-wild type cells with DNA constructs such as the Nfkb SEAP plasmid in p9kb and
validated the transfections qualitatively (Fluorescence Microscopy) and quantitatively (SEAP and Metridia Luciferase
assay)
• Designed, conducted and analyzed experiment successfully to decide the ideal DNA: transfection reagent ratio
• Successfully developed a stable cell line of HEK cells with the Nfkb driven SEAP reporter plasmid
• Performed Binding assays using 125I labeled alpha bungarotoxin to study percentage of receptor expression
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA Sept 2012 – Dec 2012
Lab Biologist
ELISA: quantification of Green fluorescent protein in protein extracts collected from Panc-I cells
cDNA preparation and PCR: used RNA from NIH-3T3 cells
MICHELLE PIRES
15 Edgerly Street, Apt # 20 ~ Boston, MA 02115 *****.*@*****.***.***
Miniprep: Isolated and purified molecular biology grade plasmid DNA from a culture of bacterial cells
Enzyme Kinetics: Determination of Vo, Vmax, Km, Competitive and Non-competitive inhibition
Maxwell Life Sciences Pvt. Ltd, Thane, India June – August 2010
Trainee in Quality Control/ Quality Assurance and Research and Development Departments
• Performed Quality control tests on various products like tablets, syrups, capsules and packaging material
• Implemented protocols and documented findings
• Handled instruments like Karl Fischer apparatus, Polarimeter, Refractometer, IR spectrophotometer, HPLC
and Digital pH meter
PRESENTATIONS/PUBLICATION
• Presented seminar on ‘Epigenetics and DNA Methylation under the guidance of Dr. Radhika Raheja at
M.E.T Institute of Pharmacy, 2009
• Poster presentation on ‘Radiation Synovectomy’ at MET TECH FEST-2009, organized by M.E.T. Institute
of Pharmacy, Mumbai, India
• Poster presentation on “Investigating anatabine’s anti-inflammatory effects through NFkb” NU Research
Expo, Northeastern University, March 22, 2013
SCHOLARSHIPS and ACHIEVEMENTS
• Prestigious Sir Ratan Tata Scholarship award for Academic Excellence Annually 2006-
2009
• Prestigious MET Scholarship for Academic Excellence January 2010
• Member of the Indian Pharmaceutical Association (IPA) August2006–
May2010
ADDITIONAL EXPERIENCE
• Residential Security Office, Northeastern University, Boston, MA
Proctor Dec 2011-Dec 2012
Check the identification cards of students residing in dormitories
Promoted to Supervisor
• Supervisor
Dec 2012- present
Supervise 4 to 8 hour shifts, go on rounds, assign stations and respond to phone calls and emergencies
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