Research Assistant / Associate, Molecular biology, In vivo

J ENIL PATEL

*** ****** ******, *** ** BOSTON, MA, 02135 857-***-**** *****.********@*****.***

EDUCATION :

NORTHEASTERN UNIVERSITY – BOSTON, MA MAY 2013

Master in Pharmaceutical Sciences

Concentration in Pharmacology – c GPA 3.44 / 4.0

Related Coursework: Biochemistry, Molecular Biology, Pharmacology I & II, Receptor Pharmacology, Pharmaceutical

Sciences lab course, Drug design and development

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES – BANGALORE, INDIA SEP 2010

Bachelor of Pharmacy – GPA 4.0 / 4.0

Related Coursework: Biotechnology, Microbiology, Pharmacology, Pharmacokinetics, Therapeutic Drug monitoring

TECHNICAL SKILLS :

CELL CULTURE: Demonstrated efficiency in maintaining diverse adherent and suspension type mammalian cell

lines such as HEK 293T and MOLM14 respectively through aseptic technique along with preparation of culture

media and buffers

MOLECULAR BIOLOGY: Ability to execute Western Blot, SDS-PAGE Electrophoresis, ELISA, DNA/RNA

purification, Maxiprep, Transfection, PCR and Protein Assays for sample analysis; familiar with performing Flow

cytometry, Plasmid preparation

ANIMAL HANDLING: Basic lab experience with dosage administration to mice via IV, IM and IP routes; familiar

with pharmacological experiments such as conducting Dose-response curve assays using isolated tissue method

SOFTWARE PROFICIENCY: Microsoft Office, ChemDraw, SPSS statistics

RESEARCH EXPERIENCE :

DANA-FARBER CANCER INSTITUTE - Boston, MA

RESEARCH TRAINEE OCT 2012 – MAY 2013

Assist a senior scientist on a project involving studies on the role of protein SET in the activity of transcription

factor CEBP-α which is associated with Acute Myeloid leukemia; observe and execute relevant experiments by

thorough understanding of the protocols in coordination with the scientist.

Passaging of cells for maintaining experimental cell lines such as MOLM14 and HEK 293T under sterile

conditions, cell counting; Performing SDS-PAGE, Western blot, ELISA and Immunoprecipitation to optimize

assay conditions including determination of effective primary antibody product for protein SET or CEBP-α and

to observe their expression under different experimental conditions; Prepare stock reagents and culture

media,

Follow protocols for cell transfection and DNA/RNA purification experiments; assist in performing PCR after

ChIP assays.

Maintain a record of experimental data along with analysis and discussion of results; and plan for succeeding

studies to be carried out.

NORTHEASTERN UNIVERSITY- Boston, MA

RESEARCH ASSISTANT JAN 2012 – MAY 2013

Prepare for a review article based upon recovery patterns of specific compounds from Graphitized carbon

black adsorbents in Solid-Phase extraction experiments.

Study relevant research articles to gather information regarding extraction conditions such as adsorbents and

elution agents used for low-recovery compounds.

UNDERGRADUATE PHARMACOLOGY LAB – Bangalore, India SEP 2009 – AUG 2010

As a part of academic curriculum, trained for In Vivo Pharmacology including mice handling, basic dosing

principles and fundamental experiments for testing efficacy of analgesics and smooth muscle constrictors.

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