Assistant University

E-mail:**********@*****.***

Wenwen Zhang M.S. Biotechnology

Tel: 949-***-****

**** ***** **. ***** ** 91710

Profile

Solid knowledge and experiences in molecular biology, biochemistry and bioengineering techniques

Skilled TnT reactions, Western Blot, ELISA, HPLC, Protein Affinity Chromatography, BCA, microfluidics devices, protein production

Proficient in DNA/RNA recombination, proteomics studies, knowledgeable in mesenchymal stem cells (MSC) homing research

Skilled experience and knowledge in dissection of mice, organ collection and compound injection

Extremely self-motivated and also able to thrive in a teamwork environment

Excellent problem solving and communication skills

Fluent in English and Mandarin in writing, reading and speaking

Education

University of California - Irvine (UCI), Irvine, CA Graduate in June 2013

M.S. in Biotechnology

University of California - San Diego (UCSD), La Jolla, CA Mar.2008 -Mar.2010

B.S. in Biological Science

Academic Publications

Micro-devices for analyzing stem cells. Microfluidic devices for biomedical applications (chapter 16). Woodhead Publishing (Cambridge, UK).

Author: Kang DK, Lu J, Zhang W, Chang E, Eckert MA, Ali MM, Zhao W.

Research Experience

Graduate Research, University of California, Irvine, CA Sep 2012-Jun 2013

Mentor: Dr. Weian Zhao, Department of Pharmaceutical Sciences

Designed and performed experiments using microfluidic devices to understand the mechanisms of MSC recruitment in stroke

Used confocal microscopic examination of stages of MSC transmigration of blood-brain barrier

Internship, Allergan Inc, Irvine, CA Jun 2012-Sep 2012

Optimization of Humanized Antibodies

Optimized binding affinity and stability of humanized antibody using mutagenesis techniques

Extensive experience with DNA isolation, PCR, bacterial culture, tissue culture of human cancer cell lines

Hands-on experience with Western blotting, protein production and protein mass spectrometry

Internship, California State University, San Diego Jan 2011-May 2011

Protein Investigation for Post-cardiac infraction

Delivered recombinant DNA plasmids into cardiac cells using viral infection for cardiac infarction therapy

Performed gene deletion to observe the function of the protein of interest

Internship, University of California San Diego Jul.2009 - Sep.2010

Investigation of gene Nanog for maintaining the pluipotency for Embryonic Stem Cells (ESCs)

Performed experiments with gene knock in/out and gene targeting with in vitro models

Hands-on experience with Cre-Recombination in transgenic mice models

Successfully transfected recombinant plasmid into ESCs to investigate the function of the gene with in vivo models

Employment History

Teaching Assistant for Molecular Biology and Biochemistry Department at UC Irvine Sep 2012-Jun 2013

Teaching Assistant for Biology Department in UC San Diego, Sep 2009-Dec 2009

Research Assistant at UC San Diego Dr. Xu Yang’s Laboratory Sep 2009-Oct 2010

Dental Assistant at Dr. Liu Xuan Dental Clinic Sep 2005-Dec 2007

Math Tutor at Mt.Sac Community College Mar 2006-Dec 2007

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