Medical Technologist

Dongxu Xie, Ph.D. MLS (ASCP)CM, MB (ASCP)CM

(Updated on Feb 22, 2013)

Address: **** ********* ****** **, *****, NY 10463 Email: *****@*******.*** Tel: 718-***-****

(H), 212-***-**** (Cell)

License: New York Medical Technologist (approved on September 1, 2011)

ASCP Certification: 1. MLS (ASCP, No. 239910)CM; 2. MB (ASCP, No. 1895)CM

Work and Research Experience

Medical technologist (02/12- ) Clinical Molecular Diagnostics and Microbiology Laboratory, James J.

Peters VA Medical Center (Bronx, NY), U.S. Department of Veterans Affairs, 130 West Kingsbridge

Road, Bronx, NY 10468 (Perform HBV, HIV, HCV viral load, HCV genotype, clinical serological assay,

clinical microbiological assay. Developed new HCV genotyping assay shown in the list of my papers.

Wrote: protocol of IL-28 mutation assay, protocol of bacteria identification based on 16S RNA

sequence)

Associate Research Scientist (06/10 - 12/12) Cancer Research Center, Columbia University, Columbia

University. 3959 Broadway, New York, NY. 10032 (perform human genomic DNA methylation assay,

leukemia and tumor immunochemotherapy research)

Staff Associate Scientist (06/08-07/09) Division of Bone Marrow Transplantation, Department of

Pediatrics, Columbia University. 3959 Broadway, New York, NY. 100**-***** (perform human

leukemia and tumor immunochemotherapy research)

Research Fellow (10/04-05/08) Center for Immunotherapy of Cancer and Infectious Diseases, MC 1601,

Department of Immunology, University of Connecticut School of Medicine, 263 Farmington Avenue,

Farmington, CT 06030-1601, USA. (perform human immune response to flu and VZV viral research)

Associate Research Scientist, 12/01-9/04 Genomic Core Lab. Kaplan Comprehensive Cancer Institute.

Medical Center of New York University. 550 1st Ave. RR814, New York, NY 10016. (Built genomic core

facility, charged, maintained and ran the core facility. Made human and mouse 30k printing expression

gene microarray chips, mosquito expression chips, mouse genomic DNA chips, wrote protocols of

microarray assay, ran Bioanalyzer, real time (RT) PCR, instructed students, technicians and faculties,

Visiting Scholarship: 9/00-9/01 Mount Sinai School of Medicine, New York, NY (Human gene therapy

research)

Postdoctoral Fellow, 10/97-7/99, National Key Laboratory of Molecular Oncology, Cancer Institute of

Chinese Academy of Medical Sciences, Beijing, China 100030 (Molecular oncological and

chemotherapy research)

Deputy Director of Hematology Clinical Examination Lab, 07/97 – 10/08 Hematology & immunology

Institute of Military Hematology Center, Department of Hematology, Lanzhou General Hospital,

Lanzhou, China. (perform clinical hematological diagnostics, ran molecular sub-typing of leukemia

using flow cytometry (CD markers) and RT-PCR (I designed this system); Laboratory diagnostics of

remained leukemia with RT-PCR that I developed methods.

Technologist, Clinical Laboratory Examination Department of Lanzhou General Hospital, Lanzhou, China.

12/89 Run ELISA, RIA, etc. Laboratory diagnostics of AFP, CEA, IFN-gamma, GH, TSH, Hepatitis A,

B (HBSAg, HBSAb, HBEAg, HBEAb, HBCAg), HIV test, biochemistry assay, CBC, urinalysis, human

karyotype assay, T-cell subtype assay, CRP, RA, Anti-O, human Igs immune diffusion assay, HCG fast

assay, etc. Developed human IFN-gamma ELISA and radioimmunoassay, rapid and simple human

karyotype preparation kit, monoclonal preparation, human gastric cancer detection using the

fluorescence labeled monoclonal Ab.

Teaching Experience

06/08- Teaching and helping clinical fellow to do scientific

1.

experiments.

10/04-05/08 Instructed summer students (college and high

2.

school) to do scientific experiments.

01/90-12/00 Instruct MS and Ph. D students for their

3.

projects.

Instructor (1986-1989), Department of Biology, Northwest

4.

Normal University (China) Undergraduate Courses: Teach Histology and experiments (including

immunochemistry), Compare Anatomy, and Embryology, Development Biology and experiments.

Skills and Techniques

Molecular and cellular Cellular Biological techniques, Flowcytometry, Flowjo, BIOCHIPS

(Microarrays) and data analysis, Confocal Microscopy, Pyrosequence, Molecular biological techniques

(Northern blot, Southern blot, West blot, Clone and Subclone, RT-PCR, Bio-Plex system, Real-time PCR,

Infection, Transfection, Expression and purification of protein etc.), Site-specific Mutation (Frt/Flp or

Lox-p/Cre) (Gene Target), RIA, Luminex, ELSA, Cell trape, Experimental animal work, Affinity

chromatography, HPLC and ESR.

Bioinformatics or computational SAS, Computer application: DOS, Windows, Mac, Windows NT, Unix

and Linux operation systems, and Microsoft Office (word, Excel, Access, PowerPoint and Publisher),

Photoshop, Cellquest, Macvector, Flowjo, computer language perl and MySQL.

Education

09/01/1994-07/28/1997 Ph.D. Physical Chemistry, Lanzhou Institute of Chemical Physics of Chinese

Academy of Sciences

09/01/1986-07/15/1989 M.Sc. Development Biology, Northwest Normal University, China

09/01/1982-07/15/1986 B.Sc. Biology, 1986, Northwest Normal University, China

Medical Laboratory Scientist Certification Training Program

09/01/2010-08/12/2011 Medical technologist program in New York Methodist Hospital.

09/12/2-12/03/2010 Blood Bank and Clinical Immunology Rotation in Sunny Down State Medical

Center

12/08/2010-03/03/2011 Clinical Microbiology Rotation in Mount Sinai Medical Center.

03/08/2011-05/19/2011 Clinical Hematology Rotation in Lenox Hill Hospital

05/23/2011-08/12/2011 Clinical Chemistry, clinical Flow cytometry, Immunology, and Urinalysis in

Mount Sinai Medical Center.

Training

11/2012- Clinical Laboratory Lead and Management Certificate Program 2 (AACC)

1.

10/2012-11/2012 Basic Principles & Architecture of Lab Information Systems Certificate

2.

Program 2 (AACC)

5/2003-6/2003 Microarray data analysis and mining, Biodiscovery Inc. CA

3.

2/2003-4/2003 Microarray operation and assay, MWG Inc. NC.

4.

10/2006-5/2007 Approaches to Data Management and Analysis of Epidemiologic Data (SAS),

5.

University of Connecticut Health Center

09/2007-04/2008 Cellular and Molecular Advanced Immunology Course, University of

6.

Connecticut Health Center

04/2009 writing NIH grant training

7.

Grants:

1998-2000 Young Scientist Foundation of Gansu Science and Technology Assn., Gansu, China

1992-1994 Foundation of Science and Technology Association of Lanzhou General Hospital

Created Assay Protocols

1.Dongxu Xie, 16S-rDNA assay protocol: Detection of bacteria in clinical samples (CSF) (2013)

2.Dongxu Xie, 16S-rDNA assay protocol: Detection of bacteria in cerebrospinal fluids (CSF) (2013)

3.Dongxu Xie, Genotype of IL-28B for Prediction of Response to Treatment of Chronic Hepatitis C

(2013)

4.Dongxu Xie. Development of RIA and ELISA of IFN-gamma and Clinical Application. 1992.

5.Dongxu Xie. Immunohistochemistry and ELISA of the new antigen associated with human gastric

cancer. 1993.

6. Dongxu Xie. Human and Mouse Array Application Manual. Genomics Core Facility of Cancer Institute,

New York University

7.Dongxu Xie. Human MIF Beads Assay Protocol, 2007. UCHC

8.Dongxu Xie. Mouse MIF Beads Assay Protocol, 2007. UCHC

9.Dongxu Xie. Radioimmunoassay of Gamma-interferin in human samples. 1994

Patent Pending Aplications:

1. Murty Vundavalli; Dongxu Xie. Methods and Compositions for Cervical Cancer Treatment. 2011

(Columbia University)

2. Dongxu Xie. A new CTL assay method.

3. Dongxu Xie, Ross Basch. A new generation microarray.

Publications

Manuscripts in Review

1.Dongxu Xie, Idamary Perozo, Darryl Williams, Azra Shahidi. Performing Roch COBAS

AmpliPrep/TagMan HCV Test with Simens LiPA HCV Amplification 2.0 for HCV Genotype.

2.Gopeshwar Nayana; Dongxu Xie; Murty Vundavalli. Epigenetic Inactivation of Decoy Receptors

Mapped to 8p12-21.3 Deleted Region Confers Sensitivity to Apo2L/TRAIL-Combination Therapy in

Cervical Cancer.

Gopeshwar Narayan, Allen J. Freddy, Dongxu Xie, Hema Liyanage, Lorraine Clark, Sergey

3.

Kisselev, Ji Un Kang, Subhadra Nandula, Catherine McGuinn, Shivakumar Subramaniyam, Prakash

Satwani, David Savage, Govind Bhagat, Vundavalli V. Murty. Promoter Methylation-mediated

Inactivation of PCDH10 in Acute Lymphoblastic Leukemia

4.Dongxu Xie, Ian Waxman, Carmella Van de Ven, Janet P. Ayello, Nancy Day, Panos N. Papapanou,

Megan Lim, Sherrie Perkins, Mitchell S. Cairo. Bortezomib (BTZ), Small Molecule IKK Inhibitor

ML120B and Combination Therapy Inhibit NF-κB Activation and Promote Multiple Apoptotic Pathways

in Primary Mediastinal B-Cell Lymphoma (PMBL).

Some Published

1. Genes, Chromosomes and Cancer. 2011 http://onlinelibrary.wiley.com/doi/10.1002/gcc.20922/pdf

2. International Immunology. 2009. Doi: http://intimm.oxfordjournals.org/cgi/content/full/dxp087?

ijkey=KlzPVPazT9zvgQh&keytype=ref

Cellular Immunology. 2009. Doi: http://dx.doi.org/10.1016/j.cellimm.2009.06.011

3.

Journal of Immunological Methods. 2009. 343:42-48. doi:10.1016/j.jim. 2009.01.008

4.

Mechanisms of Ageing and Development (2007), 128, 392-400. (MEDLINE)

5.

6. Journal of Immunology. 2006 May 15;176(10):6333-9. (MEDLINE)

Journals of Gerontology: Medical Science. 2008, Aug; 63(8):879-84. (MEDLINE)

7.

Journals of Gerontology: Medical Science. 2008 Feb; 63(2):184-9. (MEDLINE)

8.

Vaccine. 2009 Apr 21;27(18):2418-25. PMID: 19368783.

9.

10. CMJ 2002; 115 (2):280-285. (MEDLINE)

11. Chinese Science Bulletin 1999, 44(17): 1581-1586. DOI: 10.1007/BF02886096

12. Se Pu (Chromatography) 1998 Jul;16(4):327-30 (MEDLINE)

13. Journal of Chromatography B: Biomedical Applications. 688 (1997):360-363. (MEDLINE)

14. Chinese Journal of Magnetic Resonance 1997, 14(6): 523-528. (English version)

15. Dongxu Xie, Inducing Differentiation of tumor cells with chemical compounds. Ph.D. Program

Thesis. 1997

16. J of the Fourth Military Medical University, 1996, 17 (6): 418-421. (Abstract in English)(link)

17. J of Chinese Immunology. 1993, 9(4): 217-219. (Abstract in English) (LINK)

18. J of Chinese Immnology.1993, 9(3):1 43. (in Chinese)

19. J of Chinese Immunology. 1993, 9(4): 217-219. (Abstract in English) (LINK)

20. J of Chinese Immnology.1993, 9(3): 143. (in Chinese)

21. Dongxu Xie, Guixia Liang and Mingting Mao. Cytological study of the fertilizing process of the eggs

on Bufo bufo gargarizans. Zoology

Certain Papers that Used my Chips:

1. The Journal of Cell Biology, Vol. 179, No. 7, 1399-1412

2. Mol Cell Biol. 2005 Sep;25(18):8166-78.

3. Genes & development.2005;19:1499

4. Mol Cell. 2004 Nov 5;16(3):399-411.

Book Chapters and Reviews

1. Dongxu Xie, Chapter 1: Preparation of Antigens and Antibody, in Handbook of Immunological Principal

and Practice 2001. Ren Min Jun Yi Press. (In Chinese)

Seminar

Dongxu Xie, TNFRSF10D might be a critical regulator of apoptosis by TRAIL-

1.

mediated combination chemotherapy in cervical cancer. Irvin Cancer Center, Columbia University

Medical Center. Dec. 7, 2010

Dongxu Xie, Promoter methylation-mediated inactivation of PCDH10 in acute

2.

lymphoblastic leukemia contributes to chemotherapy resistance (Temporary topic, may be changed). Irvin

Cancer Center, Columbia University Medical Center. Dec. 13, 2011

Honors:

Second Prize of Science and Technique Development of Gansu Province (First Author), 2000

Third Prize of Science and Technique Development of Gansu Province (First Author), 1997

Third Prize of Military Science and Technique Development (First Author), 1994

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