High School Maintenance

GAURI INDURKAR

* ******* **** *****, ***#***, Nashua, NH-03063 347-***-****

*****.**********@*****.***

OBJECTIVE: Seeking a research position to enhance my professional and

business skills and to serve the organization with determination and sheer

commitment.

EDUCATION:

Masters in Biotechnology (NYU-Poly)

Sep'10 - May'12

Bachelors in Biotechnology (Padmashree Dr. D.Y.Patil University)

Jun'06-Jun'10

PROFESSIONAL SUMMARY:

. A biotech professional with 3 years of experience in biotech industry.

. 2+ year of experience in Microbiology, Upstream and Downstream Process

Development and Optimization and Validation.

. Experience in aseptic techniques, in vitro assays, presenting technical

papers and training students.

. Experience in Protein Extraction, Purification and Characterization.

. Extensive background in various Analytical techniques, and carrying

activities in compliance with cGMP and cGLP.

PROFESSIONAL EXPERIENCE:

Research Assistant, SyntheZyme, Brooklyn

Dec'10-Mar 2013

Fermentation of Biosurfactant based compound as a potent

Biopesticide

. Plan, operate, maintain, monitor and set up of the pilot scale

bioreactor for fermentation.

. Conducting Design of experiment studies for process development.

. Optimization of the fermentation process for industrially important

biomolecules like surfactant.

. Executed production at shake flask level followed by scale up to 1.5L

DasGip parallel Fermentor and further scale up to 5L New Brunswick

bioreactor.

. Apt using Downstream Processing techniques like Filtration,

Centrifugation, Solvent Extraction and Recrystallization of the

purified product.

. Characterization using various Bioanalytical techniques like Thin

layer Chromatography, HPLC and NMR Spectroscopy.

. Validation of the process, maintaining equipments, documenting

validation protocols and SOPs.

Modification of the Biosurfactant based compounds and Evaluation of

its Anti-microbial and Antifouling activity

. Evaluation of the biosurfactant based formulated products for its anti-

microbial activity against various bacterial, fungal pathogens by

performing in vitro assays.

. Interpretation of the microbial growth and its subsequent percent

inhibition by the biosurfactant.

. Screening for the number of live and dead bacteria using Fluorescent

Microscopy.

. Preparation of media and buffers for in vitro assays, Maintenance of

aseptic conditions (Biosafety Lab II)

. Executed and examined the propagation of the stock cultures.

Maintaining bio-inventory for cultures.

. Cryopreservation of culture stocks.

. Assisted in writing and publishing technical papers for NSF/SBIR Phase

II study.

. Represented SyntheZyme in NSF meetings and for paper presentations.

. Training High school, undergraduate and graduate students who worked

under my supervision on various Research projects and helping in

enhancing teamwork.

Bioemulsification of Hydrocarbons using Biosurfactant.

. Planning and executing emulsification of various hydrocarbons (crude

oils as well as plant oils) using biosurfactant based compound

. Comparison of these results with Chemical surfactant.

Research Intern, BioGenomics Ltd, India

Dec'09-Jun'10

Extraction and Affinity Purification of Recombinant Human Growth

Hormone and its Characterization using UV-Spectrophotometry, SDS-PAGE

and Mass Spectrophotometry.

. Developed and Optimized the purification process for the HIS tagged

Human Growth Hormone by various chromatographic runs.

. Carried out Affinity Purification by FPLC (AKTA Explorer) and Size

Exclusion Chromatography.

. Performed Quantitative estimation of the purified protein using UV

Spectrophotometry and Qualitative analysis using SDS-PAGE, LC-MS and

HPLC (Agilent).

. Attained reduced and refolded form of the purified protein.

. Hands on experience in operating UNICORN and Softmax Pro software,

worked extensively with Word and Excel.

. Written a technical report on Extraction, Purification and

characterization of hGH by various analytical techniques.

. Implemented cGMP and cGLP while conducting experiments.

PROFESSIONAL SKILLS:

Bioanalytical Techniques: LC-MS, HPLC, Flow Cytometry, FPLC (AKTA

Explorer), GC,UV-Vis Spectrometry, SDS PAGE, Western Blot, Enzymatic

Assays, In vitro Assays, Immunoassay- ELISA

Microbiology Techniques: Cell Culture, In Vitro assays: Antimicrobial

Assays, Plating techniques, Aseptic Techniques.

Technical Skills: Writing SOP, Maintenance of equipments, Activities in

compliance with cGMP/cGLP environment, Training undergraduate and graduate

students in research.

Molecular Biology Techniques: PCR, DNA and RNA Extraction

Other Skills: Organization and Analytical skills, troubleshooting skills,

can work independently, Written and Communication skills, Maintenance of

equipments.

Computer Skills: Microsoft Office- Excel, Powerpoint, Word, Outlook, Visio.

PROJECTS:

Polymerization of Polyalinine on Glassy Carbon Electrode (Electrochemistry)

Aug'10 - Nov'10

. Polymerized Polyalinine on Carbon electrode and used it as a substrate

for immobilization.

. Worked with Ion Selective Electrodes, Immunoassays-ELISA based assays.

Fermentation Efficiency Improvement:

May'08 - June'08

. Increased the fermentation efficiency of Ethanol by 0.01%.

. Validated parameters such as modification with yeast strain, storage

time of molasses, enzyme used, aeration, temperature, pH, osmolarity.

KEY COURSE WORK:

Microbiology, Immunology, Cell Biology, Bioprocess Technology, Technical

Communication, Biotech and Pharmaceutical Industry, Biochemistry

HONORS:

Polytechnic Institute Scholarship- Awarded a Merit based Scholarship for

fall 2010.

REFERENCES WILL BE PROVIDED ON REQUEST

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