Medical Research
Resume:
Vikas K Goel Ph.D.
**********@*****.***
Apt #1
Seattle, WA, USA 98115
HIGHLIGHTS OF QUALIFICATIONS
I am highly motivated, innovative, independent and multi-faceted scientist with research experience
and skills that span both basic science (cell and molecular biology, signal transduction, epigenetic
regulation, and cancer immunology) and translational research (target identification and preclinical
trials). Experience in drug discovery in collaboration with biotech/ pharmaceutical companies
(Halozyme Inc., Merck and Deciphera Pharmaceuticals) and moving therapeutic opportunity
(monoclonal antibodies and small molecule inhibitors) from a cellular concept to proof of principle
confirmation in vivo.
• Managing and leading a team of scientists, proposing and running multiple projects, toward
delivering scientific goals.
• Strong publication record in major journals.
• Productive independent researcher with excellent communication & team skills.
• Adroit at handling problems and amenable to take new challenges.
• Strong organizational skills, exceptional research experience in implementing cutting edge scientific
advances, conception, execution and data analyses of complex scientific studies.
SPECIALITIES
• Comprehensive working knowledge of signal transduction and genetic/epigenetic regulation of
genes. Oncology target identification, validation and analysis of cancer biomarkers.
• Extensive isolation and culturing of islets, Acini and ductal cells from murine pancreas.
• Developed inducible lentivirus siRNA/shRNA to target oncogenes in primary tumor cells and delivery
of viral particles to tumor in murine models.
• Expert in developing and characterization of Genetically Engineered Mouse Models (GEMMs),
orthotopic, syngeneic and xenografts (NOD/SCID and nude) for cancer drug development.
• Small animal Imaging (Ultrasound Vevo 2100 and Xenogen IVIS200), collection and analysis of
tissues and CTC (circulatory tumor cells) of preclinical trials of lead small molecule inhibitors.
• Tissue organotypic, 3D and 2D culture system (carcinoma, fibroblasts and stem cells) in defined
matrices (Collagen, Matrigel) for functional analysis and validation of chemotherapeutics.
• Receptor-ligand interaction studies (CD44, ITAV and OPN) for cellular adhesion and migration.
• Cloning of mammalian, non-mammalian full-length cDNAs (>35) and truncated proteins and
promoters, sequencing, c-DNA library construction, mutagenesis and other DNA and RNA
manipulations.
• Genetic manipulation of primary murine and human cancer cells to produce stable clones.
• Extensive experience in purification and characterization of fibroblasts, immune cells and epithelial
cells from tumors.
• Highly proficient in western blot, in vitro capture assays, Immunoprecipitation, In-situ hybridization
(FISH), Chromatin immunoprecipitation (CHIP), methylation, Acetylation, promoter-reporter assays
(fluorescent and luminescent), and EMSA.
• qPCR (real time PCR), experience in analysis of genomics/transcriptomics (microarray and miRNA)
and proteomics data (SILAC and Mass Spec).
• Collect and prepare DNA, RNA and proteins from various sources such as tumors, blood and other
body fluids of preclinical (murine model) and clinical trials (human).
• Expert in Immunohistochemistry (IHC) on frozen and FFPE tissues, tissue microarrays.
• Proficient in various imaging techniques (bright field, Confocal and multiphoton microscopy) and
quantification/analysis of IHC/IF (multicolor) stains using ImageJ, Aperio Scanscope and Nuance
quantum dot microscope.
• Cellular and molecular assays such as cell proliferation (WST-1, MTT and Brdu labeling),
Apoptosis/cell death (Ki-67, Tunnel and cleaved caspase 3) and cytotoxicity assays.
• Laser Capture Microdissection (LCM) for isolation of RNA/DNA and protein from FFPE tissues.
• Developed in vitro diagnostics PCR assay (IVD) to detect drug resistant Plasmodium from blood.
• Developed ELISA for extracellular matrix components (ECM) for cancer biomarker and diagnostics.
Developed Indirect Immunofluorescence for diagnostics of Rabies virus from clinical samples.
• Expression and purification of various recombinant proteins using bacterial, yeast, viral and
mammalian expression systems (HEK 293, NIH3T3 and Insect cell lines). Protein-protein interaction
studies (antibody antigen, and recombinant protein using Biacore).
• Expert in producing, purification and functional characterization of mouse monoclonal antibodies
(hybridoma technology) and their potential use in therapeutics and diagnostics.
• Synthetic protein production using rabbit reticulocyte expression system.
LICENSING/PATENT
• Genetically engineered mouse model of melanoma (Braf V600E) is licensed to a Biopharmaceuticals
for the development of novel immunotherapy for melanoma.
• Licensing of monoclonal antibodies against Iron Regulated Outer Membrane Proteins of
Acinetobacter baumanii and antibody against nucleoprotein of Rabies virus for development of
diagnostics (by Department of Biotechnology, India).
RESEARCH EXPERIENCE
Mouse model techniques for oncology research: Developed and characterized genetically
engineered mouse models (GEMM), subcutaneous, PDX and orthotopic xenograft (NOD/SCID and
Nude), syngenic and human primary cancer models (tumor bank) for oncology drug discovery and
translational research. I am expert in animal handling techniques such as mouse tumor inoculation
and measurement, oral, sc (subcutaneous) and iv (Intravenous) dosing, blood and tissue collection.
Protein chemistry and antibody production expertise. Studied several different proteins and
peptides employing a wide variety of biophysical and biochemical methods. Expert in expression and
purification of large number of mammalian recombinant proteins from bacteria, yeast, CHO,
HEK293T cells. Studied protein-protein interaction using yeast two hybrids and BIACore resonance
technology. Highly proficient in producing and purification of mouse monoclonal antibodies using
hybridoma technology and their use in diagnostics and therapy. Strong working knowledge of
immunoblot, CO-IP and CHIP on CHIP.
Cell and molecular biology experience: Constructed C-DNA libraries (for Yeast two hybrid
system), cloned several genes in TOPO-TA vector, bacterial, mammalian, reteroviral and lentiviral
expression vectors. Strong working knowledge of FACS analysis purification of primary cells,
invasion, migration, 3D matrix invasion morphogenesis assay, cell proliferation, apoptosis, cell cycle
and luminescence reporter assays, in vitro and in vivo drug assays. Developed several primary
murine and human cancer cell lines.
Computational and Biostatistics
Developed skill sets in analyzing genomics data using Ingenuity Systems Bioinformatics Software
(IPA analysis), protein database, KEGGs pathway, GEO, UCSC Genome browser, Integrative
Genomics Viewer (IGV) and Oncomine. Expert in FlowJo, Soft-Max pro, Adobe Photoshop and
adobe Illustrator. Expert in biostatistics analysis using, GraphPad Prism, Excel and Grafit softwares.
Managerial experience and other responsibilities:
Responsible for design, implementation and management of multiple research projects. Concurrently
advise junior postdoctoral and clinical research fellows. Train and supervise and direct the research
projects of graduate students, technical and research staff. Review and prepare grant proposals,
manuscripts, and presentations at various scientific meetings. Assist in the hiring of technical staff.
EDUCATION
• Ph.D. (Microbial Protein Chemistry) All India Institute of Medical Sciences, New Delhi,
India 2000
• M.Phil. (Molecular Parasitology) University of Delhi New Delhi, India 1995
• M.Sc. (Cell and Molecular Biology) University of Delhi New Delhi, India 1994
• B.Sc. Zoology (Hons.) University of Delhi New Delhi, India 1992
RESEARCH EXPERIENCE
University of Washington Seattle, WA
Research 2013
• Using organotypic and 3D model system studying the role of Immune cells and fibroblasts in
bidirectional cross talk with carcinoma cells in pancreas cancer.
Fred Hutchinson Cancer Research Center Seattle, WA
Research Associate 2009-2013
• Understanding the role of tumor microenvironment (extracellular matrix and fibroblasts) and
TGFβ/Smad signaling in metastasis and drug resistance of pancreatic cancer.
• Preclinical trials using small molecule targeted therapy in mouse model of PDA.
• Analysis of circulating tumor cells (CTC)/cancer stem cell from murine mouse models of cancer.
• Developing a GEMM to study role of oncogenic Kras during pancreas cancer development.
Burnham Institute of Medical Research San Diego, CA
Staff Scientist 2007-2009
• Epigenetic regulation of melanocyte pigmentation and melanoma progression biology by ATF2 and
hypoxia in genetically engineered mouse model system. The work involves extensive signal
transduction, cellular and molecular biology of ATF2.
New England Medical Center Tufts University Boston,MA
Research Associate 2005-2007
• Study the role of BRAF in melanoma biology, which involves the developing and characterization of
BRAFV600E mouse model for melanoma development and progression.
• Preclinical Trials of BRAFV600E specific inhibitors using BRAFV600E mouse model.
Massachusetts General Hospital, Harvard Medical School Boston,MA
Instructor 2004-2005
PostDoctoral Fellow 2002-2004
• Study the role of BRAF in melanoma biology, which involves the developing and characterization of
BRAFV600E mouse model for melanoma development and progression.
• Preclinical Trials of BRAFV600E specific inhibitors using BRAFV600E mouse model.
• Tumor collections from patients for the tumor bank at MGH.
Dept. of Microbiology, All India Institute of Medical Sciences New Delhi, India
Senior Research Associate 1995-2000
• Identification and Characterization of Iron regulated Outer Membrane Proteins of Acinetobacter
baumannii.
Dept. of Zoology, University of Delhi New Delhi, India
Junior Research Associate 1994-1995
• Rapid diagnostics of wild type and drug resistance Plasmodium falciparum directly from patients
blood using PCR.
HONORS and PRIZES
2007 AACR Scholar in training award.
1998 American Society of Microbiology, Scholar in training award.
1998 Indian Association of Medical Microbiologist Dr. S.S. Kelkar Memorial Award.
1995-2000 Council of Scientific and Industrial Research India, Research Scholarship.
1994-1995 University Grant Commission Graduate Aptitude Test Research Scholarship.
PEER-REVIEWED PUBLICATIONS
• Goel VK, Izeradjene K, et.al. Timing of SMAD4 mutation controls a metastatic switch in
pancreas cancer. In preparation.
• Provenzano PP, Cuevas C, Chang AE, Goel VK, Von Hoff DD, etal. Enzymatic targeting of
the stroma ablates physical barriers to treatment of pancreatic ductal adenocarcinoma. Cancer
Cell. 2012 Mar 20;21(3):418-29.
• Meera Shah*, Anindita Bhoumik*, Vikas Goel, et.al. A role for ATF2 in regulating MITF and
melanoma development (PLoS Genetics, December 3;6(12):e1001258.
• Goel VK*, Ibrahim NI, Pemberton TJ, et.al. “Melanocytic nevus-like hyperplasia melanoma
in transgenic BRAFV600E mice”, Oncogene 2009, 28(23): 2289-98.
• Goel VK, Lazar AJ, et.al. Examination of mutations in BRAF, NRAS, and PTEN in primary
cutaneous melanoma. J Invest Dermatol. 2006, 126(1): 154-60.
• Haluska FG, Tsao H, Wu H, Haluska FS, Lazar A, Goel V. Genetic alterations in signaling
pathways in melanoma Clinical Caner Research; 2006,12 (7 Pt 2):2301s-2307s
• Tsao H*, Goel V*, Wu H, Yang G, Haluska FG. Genetic interaction between NRAS and
BRAF mutations and PTEN/MMAC1 inactivation in melanoma. J Invest Dermatol. 2004,
122(2):337-41 *Equal first author Contribution.
• Wu H, Goel VK, Haluska FG. PTEN in melanoma cancer. Oncogene.2003, 22(20): 3113-
22.
• Goel VK, Li Xuerong, Liu S C, Chisthi AH and Oh SS. Sialic Acid independent invasion of
Plasmodium falciparum in red blood cell. Proc. Natl Acad Sci (USA). 2003, 100(9): 5164-9.
• Hanspal M, Goel VK, Raphel P, Oh SS.Chisthi A. Erythrocyte Calpain is dispensable for
malaria parasite invasion and growth. Molecular and Biochemical Parasitology. 2002,
122(2):227-229.
• Goel VK, Arti Kapil. Monoclonal antibodies to immunogenic Iron Regulated Outer
Membrane Proteins of Acinetobacter baumannii are bactericidal. BMC Microbiology, 2001; 1: 16.
• Goel VK, Arti Kapil, et.al. Effect of iron on growth and extra cellular products of
Acinetobacter baumannii. Japanese J. of Medical Sciences and Biol. 1998, 51(1): 25-33.
• Anita Pandey, Arti Kapil, Seema Sood, Goel VK, et. al. In vitro Susceptibility of
Ampicillin/Sulbactum and Amoxicillin/Clavulanic acid against Acinetobacter baumannii. J. Clinical
Microbiology.1998, 36(11): 3415-3416.
• Arti Kapil, Sumeeta Gulati, Goel VK, Lalit Kumar, et. al. Investigation into an outbreak due
to Acinetobacter baumannii in a high risk ward. Medical Oncology 1998, 15(4): 270-274.
• Seema Sood, Goel VK et.al. Paratyphoid fever in India: an emerging problem!
Emerging and Infectious Diseases, 1999, 5(3): 483-484.
ABSTRACTS (Selected)
• Goel VK et.al. Modulation of pancreas cancer invasion and metastasis by SMAD4/DPC4
signaling. Presented at 102nd Annual meeting of American Association of Cancer Research, 2011.
• Goel VK, Helen H Wu, et.al. Mice transgenic for BRAF V600E demonstrate phenotype
affecting melanocyte and neural lineages. Presented at 97th Annual meeting of American
Association of Cancer Research, 2007.
• Goel VK, Li Xuerong,Liu et.al. Band 3 dependent invasion of red blood cells in Plasmodium
Falciparum. Presented in Annual meeting of American Society of Tropical medicine and Hygiene at Atlanta,
November 2001.
• Goel VK and SS Oh, Liu D, Chisthi AH. Band 3 knock out mice are completely resistant to
Plasmodium infection. Presented at Molecular Parisitology Meeting (2001) at Woodshole, Boston, USA.
• Goel VK and Arti Kapil. Iron Regulated Outer Membrane Proteins of Acinetobacter baumannii are
virulence determinants. Presented in 38th International congress of antimicrobial agents and chemotherapy
Held at San Diego USA in September 1998.
• Goel VK and Arti Kapil. Opsonophagocytic activity of monoclonal antibodies against IROMPs of
Acinetobacter baumannii. Presented in 10th International Congress of Immunology held at New Delhi in
November 1998.
• Goel VK, Arti Kapil, et.al. Monoclonal Antibodies to the Iron Regulated Outer membrane Proteins of
Acinetobacter baumannii are bactericidal. Presented in the 8 th International Congress of Infectious Diseases
held at Boston USA in May 1998.
MEMBER- PROFESSIONAL SOCIETIES
American Association of Cancer Research
REFRENCES
Dr. Phil Hinds Ph.D. Dr. Ze’ev Ronai
Professor of Radiation Oncology, Professor and Associate Director
Tufts University School of Medicine SanFord Burnham Medical Research Inst.
Phone: 617-***-**** 858-***-****
email: ******@******************.*** *****@**************.***
Dr. Venu G. Pillarisetty, M.D. Dr. Paul Lampe
Assistant Professor Professor
Department of Surgery Public Health Sciences
University of Washington Fred Hutchinson Cancer Research Center
Office: 206- 6164924 Office: 206- 667-4123
email: ***@*.**********.*** email: ******@*****.***
Dr. Frank G. Haluska, M.D., Ph.D
Vice President, Clinical Affairs, ARIAD Pharmaceuticals
26 Landsdowne Street, Cambridge, MA 02139
Phone:617- 494 0400 ext 243, email: *****.*******@*****.***
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