Engineer Assistant

Hsien-Wei (Yvonne) Meng

***E Eastwood Dr., Clifton Park, NY 12065 • 607-***-**** • *****@*******.***

EDUCATION

• Ph.D. Candidate, Biomedical Engineering

Cornell University, Ithaca, NY August 2008- August 2013

• B.S., Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan September 2002-May 2006

RESEARCH EXPERIENCE

Doctoral Research: Advisor: Dr. Moonsoo Jin June 2010-Present

Thesis Project: Cell surface display of soluble growth factors for discovery of high affinity aptamers

• Interpret confocal microscopy result to model the thickness and growing process of drosophila eggs.

• Modulate film thickness of VEGF-A & its ligands for binding affinity via surface plasmon resonance.

• Establish a high-through-put and novel platform for multiplexed-yeast-cell SELEX by using Yeast Surface Display

(YSD) system to isolate specific aptamers to cancer-related molecules

• Develop and design a multi-versatile aptamer-conjugated moiety for in vivo imaging and diagnostic detection of tumor

xenografted in mice

• Engineer I-domain integrins into a stably active form against physiological ligands via yeast cell surface display and

using the engineered I domains for the discovery of aptamers that preferentially bind to the activated I domain integrins.

• Engineer recombinant ScFv-integrin for drug-carrier and drug-delivery purpose through Yeast Surface Display method.

Graduate Research: Advisor: Dr. John Schimenti August 2008-May 2010

Research Project: Functional characterization of a putative RNA methyltransferase, Nsun7, in mouse sperm

motility

• Functional genomics of mouse chromosome 5.

• Conducted forward genetic screens for mice carrying reproductive mutations that cause infertility.

Lab Research Assistant: Supervisor: Dr. Sin-Tak Chu September 2006-June 2008

Institute of Biological Chemistry (IBC), Academia Sinica, Taiwan

• Characterized expression of lipocalin family proteins in female mouse reproductive system

• Studied the role of mouse uterine lipocalins in endometriosis

Undergraduate Research: Advisor: Dr. Chun-Shun Wang July 2003-June 2006

• Established a novel detection method to Noda Virus (NNV) infection in red snapper

• Characterized and purified Noda Virus (NNV) antibody

PUBLICATIONS AND PRESENTATIONS

Journal Publications

• Meng HW, Pagano JM, White BS, Toyoda Y, Craighead HG, Shalloway D, Lis JT, Jin MM. Cell surface display of

soluble growth factors for discovery of high affinity aptamers. (Submitting to NAR in May 2013)

• Lin HH; Liao CJ; Lee YC; Hu KH; Meng HW; Chu ST. Lipocalin-2-induced cytokine production enhances

endometrial carcinoma cell survival and migration. International journal of biological sciences 2011;7(1):74-86.

Conference Presentations

• Meng HW, Jin MM. Aptamer Discovery Using Yeast Display Cell-SELEX.

BMES Annual Fall Meeting 2011, Hartford, CT.

• Meng HW, Jin MM. Creating and selecting aptamers through yeast surface display system.

BMES Annual Fall Meeting 2010, Austin, TX.

• Lin HH, Meng HW, Chu ST. The Effect of Secretory 24p3 Protein on Endometrial Carcinoma Cell Line.

Symposium of the Taiwan Society Biochemistry and Molecular Biology, 2007, Hsinchu, Taiwan

TECHNICAL SKILLS

• Optical Instrument: Light and fluorescence microscopy (confocal), Phase contrast microscopy, Surface plasmon

resonance (SPR), Biacore

• Cell biology: Mammalian cell culture, Yeast cell culture, Bacterial cell culture,, Flow cytometry, FACS,FRET,

• Biochemistry: Protein engineering, Protein production (E.coli, yeast, mammalian cells, etc), Protein assay (2-

Dimensional electrophoresis, SDS-PAGE, ELISA, Western blotting, Antibody techniques, etc), Column

chromatography (FPLC, HPLC), EMSA (Electrophoretic mobility shift assay), IHC (Immunohistochemistry), Histology

(Tissue section), Spectrophotometry, High-throughput DNA sequencing technology, Bio-conjugation

• Molecular biology: Aptamer selection, Aptamer characterization, SELEX, DNA and RNA manipulation (cloning and

plasmid construction), Recombinant antibody discovery, Molecular evolution, Expression vectors, RNAi, qRT PCR, Gel

electrophoresis, Yeast surface display, PCR, Viral inactivation, Viral filtration, Next-generation sequencing

• Animal studies: Basic mouse injections (i.p., s.c., i.v., etc), Sedation and euthanasia, Xenograft, Tissue collection and

section, Conventional histology and slide reading (scanscope), Transcardial perfusion

• Statistical program: Microsoft Excel, GraphPad PRISM

TEACHING EXPERIENCE

Graduate Teaching Assistant: Spring 2010

BIOAP4130: Histology: The Biology of Tissue

Department of Biomedical Sciences, Cornell University, U.S.A.

Undergraduate Teaching Assistant: Spring 2006

Immunology in Practice

Department of Life Sciences,

National University of Kaohsiung, Taiwan

CERTIFIED TRAININGS

• Radioactive material safety • In-gel digestion & database • Bloodborne pathogens

search training program

EXTRACURRICULAR ACTIVITIES

• Member of Taiwanese Student Association August 2008-Present

- Mentored new students from Taiwan, helped them in living and advised them in school works

• Worship leader in First Ithaca Chinese Christian Church (FICCC) student fellowship August 2011-Present

• Small group leader in FICCC student fellowship June 2011-Present

• Vice President of student fellowship in FICCC September 2012-

Present

OUTSTANDING AWARDS

• Graduate Student Fellowship, Department of Biomedical Engineering, Cornell University August 2008- Present

• Dean’s Scholarship, Department of Life Sciences, National University of Kaohsiung, Taiwan December 2011

PROFESSIONAL AFFILIATIONS

• BMES, Biomedical Engineering Society, U.S.A.

• The Taiwan Society of Biochemistry and Molecular Biology

• The Chinese Society of Cell and Molecular Biology

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