Molecular Biology, Biotech,Tissue Engineering, Cell and Tissue Culture

Suneetha Chandanam

Email: **************@*****.***

**** **** **** ***** *** # 1010 Houston, TX-77025 832-***-****

OBJECTIVE

To contribute to the achievement of the goals of the organization and to enrich my knowledge in

exciting field of Biomedical Engineering and Biotechnology.

EDUCATION

Master of Science in Biomedical Engineering (MSBE), Genomics, Proteomics and Bionano

Engineering Major, University of Houston, Houston, Texas, Graduated in May 2013,

Cumulative GPA - 3.42

Bachelor of Technology in Biotechnology (B.Tech), Andhra University, India, May 2009,

Cumulative GPA – 3.53

PROFESSIONAL EXPERIENCE

RESEARCH ASSISTANT, Department of Biotechnology (DBT), India Ltd. India, May 18th, 2009-Aug

16th, 2011

Determined the Biomarkers that are expressed on kidney transplanted patients’ PBMCs.

Analyzed the in vivo pattern of the rejection or acceptance of transplant in different

recipients with different donor combinations based on HLA typing.

Verified the antibodies to block the biomarkers that are expressed on PBMCs of

transplanted patients such that the immune response would be diminished.

Prepares biological assays and conducts basic quantitative and qualitative analysis.

RESEARCH ASSISTANT INTERN, Tulasi Seeds Pvt Ltd, India, April 17, 2006 – July 11th, 2008

Performed BT cotton – Assays for detection of BT genes & zygosity using PCR.

Screened for Cry 1Ac and Cry 2Ab proteins using ELISA.

Practiced bacterial cell culture techniques to maintain the BT (Bacillus thuringiensis) cell

stocks for Genetic engineering experiments.

Practiced all GLP (Good laboratory practice) guidelines and regulations for research

laboratory.

Under direct supervision, performs standard research assignments involving a series of

related operations.

TECHINCAL CERTIFICATIONS

BD Biosciences certified operator of BD LSR II FACS machine.

Department of Biotechnology (DBT), India Ltd certified trainee in techniques of Mo lecular

Biology and Immunology like RT-PCR, ELISA and other Immunoassays, FACS, WESTERN

BLOTTING, PROTEIN/ ANTIBODY QUANTIFICATION, CLONING, CELL and TISSUE CULTURE

Techniques.

RELEVANT COURSE WORK

Cell and Molecular Biology

Stem Cell Engineering and Regenerative Medicine

Protein Engineering

Bio-Medical Informatics

Gene and Genome Evolution

Biostatistics

Human Physiology

TECHNICAL COMPUTING SKILLS

PRESENTATION TOOLS: MS Word, PowerPoint, Excel, Microsoft Vision

PROGRAMMING LANGUAGES: C & C++

ENGINEERING SOFTWARE’S: R, MATLAB, Auto CAD, and MathCAD.

OPERATING SYSTEMS: UNIX, WINDOWS (9 xs, NT, XP, 2k)

ACADEMIC PROJECTS

Utilizing a PEG hydrogels with the Addition of IPS cells to Create a 3D Heart Tissue Construct

Engineered and constructed a 3D polymer scaffold from PEG hydrogels through

nanofabrication.

Inoculated the scaffold with adipose tissue derived iPS cells.

Differentiated and continuously conditioned the 3D tissue by micro perfusion and

vasculature.

Screened for Invitro and in vivo functional and biological performance metrics of the

matured 3D tissue construct.

Extraction of Piperine from Pepper & its related Clinical Trials

Extracted and purified Piperine protein from black pepper through distillation.

Conducted double blinded clinical trials to test for clinical efficiency of Piperine in

improving the bioavailability of nutrients from food.

Next Generation Sequencing

Sequenced 1149 nucleotides long apr [BamP] gene that codes for Alkaline Proteases in

Bacillus subtilis using SMRT (Single Molecule Real time) DNA sequencing based Illumina.

Carried out the cost-effective analyses using SOAPdenovo.

Cardiac Tissue Engineering

The role and applications of Tissue Bioreactors in experiments to artificially grow the

cardiac myocytes and tissue are analyzed.

The non-linear differential equations are solved using MATLAB.

WORK EXPERIENCE

GRADUATE ASSISTANT 24by7 ECC (Engineering computer center) Laboratory, University of

Houston, USA, Jan 2013-May 2013

Installed Software used in the computing lab and kept them updated.

Explaining, Clarifying and assisting students with their doubts in using software like

MATLAB, UNIX, and MATHEMATICA.

Managing and assisting professors in conducting classes to students for computer and/or

software based courses.

STUDENT ASSISTANT 24by7 Office of Housing & Residential Life, Wayne State University, USA,

October 2011 – January 2012

Assisting students and professors in checking in and checking out for on-campus residence.

Accepting mails and packages for residents from USPS, FedEx, and UPS etc.

Monitoring the premises of residential building continuously for residents’ safety and

security.

HONORS AND CAMPUS ACTIVITIES

Member of GISO (Graduate Indian Student Organization) and actively participated in

conducting “Parichay ’13”, an Indian Cultural festival.

Won the BITP (Biotech Industrial Training Program) Scholarship funded by DBT

(Department of Biotechnology) for academic excellence at Sandor Proteomics Pvt Ltd.,

2009-2010.

Recruited in BCIL (Biotech Consortium India Limited) for BITP, November 2009.

Won first prize in Paper presentation competition at “BIOMICAL 2008” a National Lev el

symposium of Biotechnologists, Gandhi Institute of Technology and Management (GITAM).

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