Project System

Weijie Zhao

**** **** ****** *.*. Washington, D.C. 20007-1703

Phone: 202-***-**** E-Mail: ****@**********.***

Nationality: Chinese Birth Date: January 1st, 1990

Summary of Quantification

Energetic self-starter with over 2 years academic and hand-on experience in biochemistry, molecular biology with

background in chemistry and pharmacy; independent and effective researcher with ability to organize multiple

projects, analyze results and solve problems; proficient calibrate and operate various laboratory instrument as

well as related computer software; Laboratory skills include, but are not limited to:

Molecular Biology: recombinant DNA cloning, PCR/qRT-PCR, in vitro one-hybrid assay, yeast two-

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hybrid screening, DNA isolation and RNA extraction;

Biochemistry: recombinant protein expression and purification, protein quantization with Bradford

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method, ELISA, western blot analysis, Southern blot;

Cell Biology: mammary cell culture, DNA and RNA transfection, cell lysis techniques,

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immunohistochemistry (IHC), in situ hybridization, Annexin V assay;

Instrumentation: UV-Vis spectroscopy, fluorescence microscopy, HPLC system, protein and nuclear

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electrophoresis system, microplate reader, autoclave, microcentrifuge.

Education

Master of Science in Biochemistry & Molecular Biology 2012-2013

Georgetown University, Washington DC

• Cumulative GPA 3.7/4.0

Bachelor of Science in Pharmacy 2008-2012

East China University of Science and Technology, Shanghai China

• Cumulative GPA: 3.4/4.0; Major GPA: 3.6/4.0

The Second Prize Scholarship, Recipient

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Professional Experience

Georgetown University Medical Center Washington, DC January 2013 – Current

Jones’s Lab is a biochemical and immunological laboratory that focused on a recombinase protein RAG1 that

mutations or deletions within the core domain of RAG1 are associated with severe immune deficiency in human

patients. This laboratory as the pioneer in this specific field equipped with extensive experience and cutting-edge

facilities.

Research Intern– Biochemistry and Molecular Biology

First demonstrated that a RAG1 construct including the ubiquitin ligase domain is active in the one-hybrid

assay; developed the in vitro one-hybrid DNA binding assay that will be useful for determining whether the

RAG1 allele mutations in this domain associated with Omenn Syndrome directly or indirectly interfere with

sequence-specific binding.

Highlights:

Constructed RAG1 fusion protein and a reporter system based on DNA recombination technique

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Proficient operations for mammalian pro-B cells experiments, such as transfection and cell lysis for assays.

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Performed statistical analysis on triplicate results, analyzed data and presented in Excel

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Ability to design experiments and problem-solving skills

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National University Student Innovation Program Shanghai, China September 2010 – June 2012

Team leader and Program Researcher - Pharmacology

Project #1: the Farnesyl transferase (FTase), was as a drug target which has been identified in the study of cancer

signal pathway. This project was to discover novel antitumor lead compounds Faresyl transferase inhibitors

(FTIs) with high inhibitory activity by screening from TA02A candidate compounds.

Highlights:

Expressed and purified vectors pRSFDuet-FTaseα/β to obtain high active FTase protein

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Optimized protein purification conditions, such as imidazole concentration of elution

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Identified the optimal FTase enzymatic activity by Bradford method

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Preliminary selected 8 of 58 compound with high inhibitory activity by analyzing the IC50 value

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Project #2: Selective estrogen receptor modulators (SERMs) are a class of compounds that act on the estrogen

receptor (ER) either as agonist or antagonist different in various tissues. This work adapted the strategy

combining yeast two-hybrid assays and molecular dynamics (MD) to discover potential selective ER ligands.

Highlights:

• Demonstrated that the Y2H system was active and effective for screening ER ligands by analyzed the

EC50 value of Estradiol as the positive control and IC50 value of Tamoxifene as the negative control.

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