Ph.D. candidate with experience in drug discovery and cell biology

PRASAD SUBRAMANIAM

*** ********* ***** • Edison, NJ 08817

215-***-**** (cell) • ******.***********@*****.***

SUMMARY: A highly motivated chemist and chemical biologist with extensive research experience in nanotechnology, cell

biology, molecular biology, target-based drug discovery and oncology. Proven track record of problem solving, project

management and publishing in high-impact peer reviewed journals. Excellent communication and leadership skills with

documented ability to work as part of an interdisciplinary team. Highly adaptable and embraces challenges with unrivaled

enthusiasm.

EDUCATION:

Ph.D. Candidate in Chemical Biology 2007-present

Rutgers, The State University of New Jersey

Anticipated Graduation: Aug 2013

M.Sc. in Inorganic Chemistry, 2005-2007

University of Delhi, Delhi, India

B.Sc. (Hons.) in Inorganic Chemistry,

Kirori Mal College, University of Delhi, Delhi, India 2002-2005

RESEARCH/PROJECT MANAGEMENT EXPERIENCE:

Doctoral Student, Rutgers University, Piscataway, NJ; PI: Prof. Ki-Bum Lee 2007-2013

Project: Formulation of novel drug delivery systems for the delivery of multiple anticancer therapeutics to tumor cells.

Developed nanomaterial-based platforms for the co-delivery of small molecule histone deacetylase (HDAC), tyrosine kinase

and topoisomerase II inhibitors to cancer cells in a controlled manner for synergistic induction of apoptosis

Also developed and tested a novel polypeptide-based vehicle for the co-delivery of siRNA and anticancer drugs for

synergistic cancer therapy

Extensive knowledge of signaling pathways implicated in cancer progression and metastasis, especially in aggressive tumors

such as glioblastoma multiforme (GBM) and breast cancer

Extensive knowledge of biochemical processes with in-depth understanding of the underlying signaling pathways

Strong technical background in molecular biology, cell biology, and biochemistry including extensive experience in

mammalian cell culture, RNAi, fluorescence microscopy, flow cytometry, western analysis and protein-activity assays

Capable of working independently and in multi-disciplinary teams as a problem solver with excellent interpersonal &

project management skills; experienced in interaction with global colleagues in Japan, UK, Brazil and South Korea

RELEVANT SKILLS:

Technical

Skilled in cell biology and biochemical techniques including mammalian tissue culture (splitting, seeding, feeding, cell

counting), bacterial amplification, protein purification, RNA extraction, plasmid purification, blotting techniques (Western

and Northern blotting), SDS-PAGE, reverse transcriptase polymerase chain reaction (RT-PCR), enzyme-linked

immunosorbent assay (ELISA), immunostaining and flow cytometry

Ability to analyze and interpret data using imaging and quantitative software packages including Origin Pro, Photoshop,

CorelDraw, ChemDraw, ImageJ and Microsoft Office.

Working knowledge of basic synthetic organic techniques including small molecule syntheses, characterization and

purification.

Well-versed in the formulation of colloidal controlled release technologies including nanomaterials and their applications for

biomedical applications.

Sound technical knowledge of several physical/chemical characterization techniques with proficiency in data interpretation

including: infrared spectroscopy, nuclear magnetic resonance spectroscopy, ultra violet/visible spectroscopy, high pressure

liquid chromatography (HPLC), dynamic light scattering, zeta potential, fluorescence microscopy, scanning electron

microscopy and transmission electron microscopy

Experienced in implementing standard laboratory safety procedures to maintain a safe working environment.

Experienced in supporting and complying with GLP and GDP regulations

Communication

Wrote and published research and review papers in high impact, peer-reviewed journals

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Excellent writing skills. Involved heavily in writing grants and patents for Ph.D. advisor resulting in several successful grants

including the NIH Director’s New Innovator Award (2009) and NSF-CPSC (Nanotoxicology) Award (2012)

Excellent communicator with oral and poster presentations at local, national and international conferences (supported by

several travel awards). Presented research findings at the International Symposium in Shizouka, Japan (2011)

LEADERSHIP AND LAB MANAGEMENT EXPERIENCE

Lab Manager and Lab Safety Officer (August 2008-present): Was responsible for overseeing the day-to-day

operations of the lab along with the Ph.D. advisor. Trained new graduate and undergraduate students in maintaining

good lab practices (GLP) and good documentation practices (GDP). Also took part in departmental safety meetings in

order to ensure implementation of safety regulations as per University and US-EPA norms

Graduate Student Mentor, Rutgers University (August 2008 – Present): Mentored 10 high school and undergraduate

students with majors including chemistry, biology, pharmacy, and biomedical engineering; Students were from

organizations including Rutgers Aresty Program, National Science Foundation Integrative Summer Undergraduate

Research Frontiers (NSF-ISURF) Program, The Research In Science and Engineering at Rutgers (RISE) Program and

National Science Foundation CENTC High School Outreach

Excellent negotiation skills and business acumen developed through setting up the lab for Ph.D. advisor as his first

graduate student. Heavily involved in budget preparation, equipment ordering and negotiating with companies for

pricing

TEACHING EXPERIENCE

Head Teaching Assistant for Organic Chemistry Lab, Rutgers University (2011-2013); Planned and managed the

Organic Chemistry Lab as head teaching assistant (TA) for over two years. Supervised and trained about 15 graduate

TAs every semester for teaching and managing labs

Teaching Assistant for General Chemistry and Analytical Chemistry Lab, Rutgers University (2007-2011)

SELECTED PUBLICATIONS AND PRESENTATIONS:

Subramaniam P, Barman DN, Lee K.-B. “Multifunctional dendritic polypeptide-based nanocarrier for delivery of

anticancer drugs and siRNA to brain tumor cells. Submitted

Subramaniam P, Lee SJ, Shah S, Patel S, Starovoytov V, Lee K.-B. “Generation of a library of non-toxic quantum dots

for cellular imaging and siRNA delivery”. Advanced Materials (2012), 24, 4014–4019

Subramaniam P, Shah BP, Jung GY, Lee K.-B. “Polymer-based approaches for tissue engineering and regenerative

medicine”. Polymer Science and Technology (2011), 22, 1-6

Park JK, Jung J, Subramaniam P, Shah BP, Kim CJ, Lee JK, Cho J.-H, Lee C, Lee K.-B. “Graphite-coated magnetic

nanoparticles as multimodal imaging probes and cooperative therapeutic agents for tumor cells”. Small (2011), 7, 1647–

1652

Kim CJ, Shah BP, Subramaniam P, and Lee K.-B. “Synergistic induction of apoptosis in brain cancer cells by targeted

codelivery of siRNA and anticancer drugs”. Molecular Pharmaceutics (2011), 8, 1955–1961

Prasad Subramaniam, Birju P. Shah, Ki-Bum Lee. “Multifunctional polypeptide-based nanocarriers for targeted drug

and gene delivery to tumor cells”. MRS National Meeting, Spring 2012. Poster Presentation

Prasad Subramaniam, Birju P. Shah, Ki-Bum Lee. “Multifunctional nanoparticles as imaging probes and cooperative

therapeutic agents in tumor cells”. Shizuoka University International Symposium, Shizouka, Japan. Poster Presentation

PATENTS:

Prasad Subramaniam and Ki-Bum Lee. “Graphite-coated magnetic nanoparticles as multimodal imaging probes and

cooperative therapeutic agents for tumor cells”. Patent Pending

Prasad Subramaniam and Ki-Bum Lee. “Generation of a library of non-toxic quantum dots for cellular imaging and

siRNA delivery and applications thereof”. Patent Pending

Cheoljin Kim, Birju P. Shah, Prasad Subramaniam and Ki-Bum Lee. “Cyclodextrin-modified polyamines for

synergistic inhibition of tumor cell proliferation via co-delivery of siRNA and anticancer drugs”. Patent Pending

SELECTED HONORS AND AWARDS:

William Rieman Award for Outstanding Performance as a TA (Organic Chemistry), November 2012

Thomas Duff Travel Award for poster presentation at the MRS Spring 2012 National Meeting at San Francisco, CA

Best Poster Award in the Bioscience Division, Shizuoka University International Symposium (2011), Shizouka, Japan

Rutgers University Graduate School Travel Award for Oral presentation at the ACS Fall 2011 National Meeting at

Denver, CO

Third position in the Annual Science Aptitude contest for undergraduate students (QUEST), University of Delhi, Delhi,

India

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