Assistant Project

SHUBHLAKHAN KAUR DHILLON

*** ******** ******, ***-**, Boston, MA 02115 916-***-**** *******.*@*****.***.***

SUMMARY:

Reliable, hardworking, and dedicated team player who works well under pressure and with minimum supervision. Fast learner with

ability to adapt to new situations.

EDUCATION:

Masters in Pharmaceutical Sciences Expected May 2013.

Northeastern University - Boston, MA.

Relevant Coursework- Biochemistry, Molecular Cell Biology, Advanced drug delivery systems, Advanced physical pharmacy,

Pharmacokineticas and drug metabolism, Introduction to Nano-medicine, Drug design, evaluation, and development, Research Ethics.

Bachelor of Pharmacy July 2011.

Punjabi University, India.

LAB SKILLS:

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Cell culture Techniques ELISA Western Blot

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Animal handling (mice) PCR Plasmid Preperation

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Flow Cytometry Immunofluorescence Electroporation

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Column chromatography Inverted Fluorescence Site directed mutagenesis

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Microscopy

RNAi Multicellular 3D spheroid

•

• Ex-vivo tissue processing culture

Gel-Electrophoresis

• •

• Cell based assays GLP

UV/VIS Spectrophotometry

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• HPLC

Nanodrop

RESEARCH EXPERIENCE:

In- vitro Pharmacologist at Cardio- vascular, metabolic, endocrine disease unit, Pfizer Inc. Nov 2012- present

• Maintain and characterize various mammalian cell lines like THP1, HEK293, JURKAT, CHO and others.

• Perform site directed mutagenesis and prepare plasmid to transfect cell lines.

• Process mice tissues ex vivo to prepare them for further experiments.

• Purify and quantify RNA from tissue lysate. Perform RT-PCR for the purified RNA and analyze the Taqman results.

• Perform various cell based assays for different cellular components on the tissue lysate.

• Perform Western Blots and ELISA for the proteins of interest and analyze the results.

• Purify and quantify DNA from microbial cultures transfected with gene of interest.

Centre for Pharmaceutical Biotechnology & Nano-medicine. Jan 2012 – Oct 2012

Northeastern University (Dr. Vladimir Torchilin’s lab)

Graduate research assistant

“Optimizing multicellular co-culture spheroid model to closely resemble in-vivo cancer cell environment”

• Optimized ratios of human fibroblast and human lung cancer cell line to prepare spheroids.

• Checked cytotoxicity of various free and micellar drugs on these co-culture spheroids and on same ratio of monolayers.

• Compared drug cytotoxicity between co-culture and single cell line spheroids.

• Performed western blot to prove the presence of two cell lines in spheroids.

• Prepared immunolabelled slides of spheroid sections.

“Preclinical cytotoxicity studies of drug loaded lipid nanoparticles on cancer treatment”

• Prepared drug micelle and calculated the drug encapsulation efficiency.

• Analyzed micelles for size and zeta potential using zetasizer.

• Split and maintained various mammalian cancer cell lines.

• Extracted mice brain to study blood brain barrier penetration of drug formulation using HPLC.

• Prepared sample for FACS and reviewed them using flow cytometer for cell cycle study.

• Prepared conjugate for micelle formation and purified it by size exclusion column chromatography.

• Fractionated antibody and analyzed the sample after column chromatography using nanodrop or performed BCA assay.

Academic Project:

Punjabi University, Patiala (India)

“Study effect of various CNS drugs on mice” Jan-April 2009

• Gave intraperitoneal injection of drugs to mice.

• Observed activity of mice on various instruments like Rota rod, swimming technique, plus maze.

TECHNICAL SKILLS:

• Microsoft Excel, Access, Word, PowerPoint, Softmax Pro 5.4, Graphpad Prism, FlowJO, Multigauge V3.2, SDS 2.3,

RQManager1.2.

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