Product Development Manufacturing

Sanchit Trivedi *******.*******@*****.***

** ***** **** **., ******, MA 01854 978-***-****

SUMMARY

Graduated with MS in Biological Sciences specializing in Biotechnology. Over three years of industrial and academic

research experience in protein purification, Analytical techniques, Cell culture, Molecular Biology, and Assays (cell based).

KEY SKILLS

Analytical/Assays

MTT Cell Proliferation assay and Bradford assay for determining protein concentration

Quantitative fluorescence Microscopy for beads analysis and Confocal Microscopy

GST column and affinity chromatography to monitor the production of the recombinant protein

Flow cytometry (guava easyCyte Flow Cytometer) and high throughout sequencing data: Analysis

Buffer Preparation, Protein purification using Affinity Chromatography, Centrifugation, Dialysis, Ammonium sulfate

and MnCl2 precipitation.

Proficient in liquid sample handling, including pipetting, transfer, serial dilution, and solution preparation.

UV, Visible and IR spectroscopy analysis of pharmaceutical drugs.

Immunology

ELISA for screening immunoglobulin antigens and micro ELISA for screening hybridomas

Cytotoxicity technique with antibody and complement

Column chromatography-DEAE agarose AFFI gel for separation of IgG from other immunoglobulin’s

Hem agglutination reaction, Cardiac puncture and spectroscopy

Cell/Tissue culture

Proficient in handling suspension and adherent cultures at different scales like multi-well plates and shake flasks

Culturing mammalian cells: Snap thawing, passaging and cryopreservation

Transient transfection, induction of differentiation, Wright and Giemsa staining

Generation of adult stem cells from murine bone marrow, Immunohistochemistry and Co-culture of hepatocyte

and fibroblast

Molecular Biology

Recombinant production of fusion proteins: Primer designing, transformation of competent E. coli cells with

recombinant constructs, DNA and RNA purification, Southern and Western blot, DNA sequencing, SDS -PAGE,

PCR, Restriction enzyme digest, next generation sequencing and Emulsion PCR.

Computer skills

Microsoft Office Suite (Excel, word, PowerPoint), Guava soft software (Millipore), metamorph (Image analysis), SAS

(statistical analysis software), Experienced with SAP

ACADEMIC PROJECTS

Isolation, Purification and Determination of protein content and enzyme activity of wheat germ acid phosphatase

UML Sep ’11-Dec ’11

Isolation and purification of the enzyme wheat germ acid phosphatase (WGAP) from wheat germ lysate and then

studying the kinetics of the enzyme using a time dependent assay.

Performed Gel electrophoresis and alkaline phosphatase assay to determine identity and activity at each

purification step.

The protein content was measured in the course of purification process using Bradford Assay.

Differentiation of Myoblasts on AML cell line with ATRA and DMSO and their morphological analysis UML Jan

’12 - May ’12

Determined the effect of different exposure time and concentration of all trans -retinoic acid (ATRA) on AML cell

line where treatment showed differentiation of the AML cells to mature neutrophils.

Compared their growth and morphological changes by cytospins and Wright and Giemsa staining.

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Sanchit Trivedi *******.*******@*****.***

64 Mount Hope St., Lowell, MA 01854 978-***-****

Cloning and expression of Paramecium Bursaria Chlorella Virus 1 (PBCV-1) gene to produce a desired

fusion/tagged protein UML Jan ’12 – May ‘12

Cloning and Transformation of competent E.coli cells with a gene from chlorella virus using expression vector

pMAL-c5E and induce expression of the protein encoded by the gene of interest.

Performed Primer designing, PCR of a gene from Chlorella virus, cloning of the amplified gene from into pMAL-

c5E vector, restriction enzyme screening, gel electrophoresis to analyze DNA, Southern blot, transformation of the

cloned vector into E.coli, protein expression, affinity purification, SDS-PAGE and western blotting

PROFESSIONAL EXPERIENCE

Molecular Biology Intern Ion Torrent, Life Technologies Beverly, MA Jun ’12 – Sep ’12

Product Development and automation

Development of Ion Torrent semiconductor chip technology, Ion Proton System and Ion Personal Genome

Machine® (PGM) Sequencer to deliver fast and efficient sequencing.

Product development for template bead preparation, emulsion PCR optimization, bead enrichment, Buffer

preparation and optimization and loading protocols for the PGM.

Validated both human and bacterial DNA libraries, Polymerase enzymes to get the b est possible sequencing on

PGM and performed SDS-PAGE, Southern Blotting, W estern blotting and Fluorescent Microscopy.

Installed and validated the new Ion OneTouch II instrument (Automated emulsion PCR).

Clinical Business Associate Unichem Laboratories Ltd Mumbai, India Jan ’11 – Jul ‘11

Studied clinical trials and shared scientific findings to enable pharmaceutical clients in developing marketing

strategies.

Pharmacist Emcure Pharmaceuticals Ltd Pune, India Sep ’09 - July ‘10

W orked in R&D of Tablets and Capsules under compression department, validation and optimization of new compression

machines (Legacy and Cad press), validation of new batches of tablet and capsules for manufacturing according to GMP.

Pharma Intern Watson Pharmaceuticals Ltd Goa, India May 2008 - Aug 2008

W orked in tablet and capsule manufacturing and documented batch manufacturing records.

EDUCATION

MS in Biological sciences (Biotechnology) University of Massachusetts, Lowell May ’13

Coursework: Cell Culture, Molecular Biology, Biochemistry, Immunology, Advanced Protein Chemistry, Isolation and

purification of biotech products, and Advanced Analytical Chemistry.

Bachelor of Pharmacy Nagpur University India Jul ‘09

AWARDS AND ACHIEVEMENTS

President of IDRF (Indian Development and Relief Fund) UML Jul ‘12- May ‘13

Elected by the undergraduate and graduate students, Chief duties included fundraising for Indian students and for

social benefits. Help international freshmen acclimatize to the new environment and culture

Provost's Scholarship UML Aug ‘11- May ‘12

Awarded to a selected number of qualified students in their first year

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