Medical Device High School

SAURABH TRIKHA **** Virginia Avenue NW, Apt C-***,

www.linkedin.com/pub/saurabhtrikha/24/434/365 Washington DC-20037.

202-***-**** *********@*****.***

Education:

Doctor of Philosophy(PhD) in Biological Fall 2008-August 2013

Sciences - GPA 3.86

George Washington University.

Bachelor of Technology (B.Tech.) Fall 2004- Fall 2008

-Biotechnology - GPA 3.4

Guru Gobind Singh Indraprastha University, Delhi, India

Secondary and High School - 1st division Fall 2003

Ramjas School Pusa Road, Delhi, India

Relevant Coursework: Life Sciences, Cell and Molecular Biology, General and

advanced Biochemistry, Microbiology and Immunology, Plant, Animal and

Environmental Biotechnology, Food, Enzyme and Industrial Technology,

Chemical Engineering and Bioenergetics, Biosafety, Bioethics and IPR in

Biotechnology, Recombinant DNA technology and stem cells in health care,

Commercialization, Marketing and Management of Biotechnological Products,

Diagnostic Techniques.

Objective:

A final year PhD candidate with a diverse array of knowledge in cell,

molecular biological and biochemical techniques coupled with equal

understanding of different computer operating systems including MS Office,

Graphic Prism Software, Image J, Zeiss Software and Adobe Photoshop along

with excellent lab organization and writing skills. Seeking full

time/contract/positions in life sciences, pharmaceutical, biotech,

diagnostics, medical device companies and academic institutions.

Skills:

1. Hands on experience with working and developing cellular, molecular

and biochemical experiments.

2. Strong oral, written and presentation skills

3. Excellent analytical and problem-solving skills.

4. Training/ mentoring undergraduate students in a biological research

laboratory.

5. Teaching experience at the university level.

6. Good team-player in industrial and university environments.

Industrial Working Experience: 2 years

1. June 2006 - August 2006; handling and practicing cellular, molecular,

biochemical techniques as a summer trainee at National Bureau of Plant

Genetic Resources and Ranbaxy Laboratories, Delhi, India.

2. August 2006 - February 2007; understanding and practicing pathological

techniques as a summer trainee at Apollo Clinic and Charitable medical

health center, Delhi, India.

3. June 2007 - September 2007; successfully accomplished a project on DNA

diversity in jatropha species using ISSR markers at National

Bureau of Plant Genetic Resources (NBPGR), Delhi, India.

4. October 2007 - June 2008; successfully accomplished a project on

regulon biotechnology in rice plants at school of biotechnology, Guru

Gobind Singh Indraprastha University (GGSIPU), Delhi, India.

Teaching Experience: 2 years

Graduate Teaching Assistant, Biological Sciences-George Washington

University

1. Fall 2008 - fall 2009 Instructor, Diversity of life, Bisc 3,

Department of Biological Sciences, GWU.

2. Fall 2009 - fall 2010 Instructor, Diversity of Life, Bisc 4 and Bisc

5, Department of Biological Sciences, GWU.

3. Fall 2010 - fall 2011 Official Tutor for undergraduates at Columbian

College of arts and sciences, GWU.

Responsibilities: Taught undergraduate biology laboratory courses;

conducted discussion and laboratory sessions, held regular student office

hours, and proctored and graded exams for the professors.

Research Experience: 5 years

Graduate Research Assistant, Biological Sciences - George Washington

University

1. Fall 2008-fall 2009 Physiological role of human amylin in type 2

diabetes

2. Fall 2009-fall2011 Pathological role of human amylin in type 2

diabetes.

3. Fall 2011-fall2012 Elucidating the internalization pathways of human

amylin in pancreatic cells

4. Fall 2012-fall2013 Identifying the proteolytic compartments in

degradation of human amylin in pancreatic cells.

Technical Areas of expertise:

1. Optical, fluorescence and confocal microscopy.

2. BLASTP/FASTA, CLUSTAL-X/W, EMBL database, Gene Doc, Genebank,

Bibliographic dB

3. UV/VIS Absorbance spectrophotometer, Ultracentrifugation, Sucrose

Density Centrifugation, Filtration, Precipitation.

4. DNA, RNA extraction, cDNA synthesis, transformation, plasmid

isolation, PCR, and Agarose gel electrophoresis.

5. ELISA, Flow Cytometry, SDS PAGE, Western Blot, and Immuno-

precipitation.

6. Protein Biochemistry (expression, purification and characterization).

7. Antibody labeling, Fluorescent tagging and transfection approach both

in vitro and in vivo.

8. Working with mammalian cell lines and human tissues, maintaining their

sterile cultures and cryopreserving them.

Scientific Publications:

1. Cho WJ, Trikha S and Jeremic AM.2009. Cholesterol Regulates Assembly

of Human Islet Polypeptide on Model Membranes. Journal of molecular

Biology, 393-765-775.

2. Trikha S, Lee EC and Jeremic AM. 2010. Cell Secretion: Current

Structural and Biochemical Insights. The Scientific World Journal, 10,

2054-2069.

3. Trikha S and Jeremic AM.2011. Clustering and internalization of toxic

amylin oligomers in pancreatic cells require plasma membrane

cholesterol. Journal of Biological Chemistry, 41-36086-97.

4. Trikha S and Jeremic AM.2013. Endocytosis and Translocation of human

amylin monomers and its cytotoxic oligomers in pancreatic cells. PLoS

ONE (submitted).

5. Trikha S and Jeremic AM. 2013. The role of proteolysis in human amylin

turnover and toxicity in pancreatic cells. Journal of Biological

Chemistry (submitted).

Abstract Citations:

1. Clustering and internalization of toxic amylin oligomers in pancreatic

cells require plasma membrane cholesterol. Trikha S and Jeremic AM.

2. Characterization of Amylin Receptor and Amylin-Regulated Insulin

Secretory Pathways in Rat Insulinoma and Human Pancreatic Islet Cells.

Yasemin Cagil, Saurabh Trikha and Aleksandar Jeremic.

3. Cross-Talk between Proteosomes and Lysosomes Determines the Fate of

Amyloid Peptides in Pancreatic Cells. Trikha S and Jeremic AM.

Oral and Poster presentations:

1. George Washington University Research Day, Washington DC, 2010:

Cholesterol Mediated Amylin Internalization and Degradation of

Cytotoxic Amylin Oligomers in the Pancreatic Beta-Cells. Trikha S and

Jeremic AM.

2. Symposium of Protein Society, San Diego, 2010: LDL Inversely Modulates

Amylin Aggregation and Toxicity in Cultured Rat Beta-Cells. Trikha S

and Jeremic AM.

3. Mid-Atlantic Diabetic Symposium, NIH, Bethesda, Maryland, 2010: Role

of LDL in Amylin Aggregation and Toxicity in Cultured Rat Beta-Cells.

Trikha S and Jeremic AM.

4. American Society of Cell Biology, Denver, Colorado, 2011: Clustering

and internalization of toxic amylin oligomers in pancreatic cells

require plasma membrane cholesterol. Trikha S and Jeremic AM.

5. Endocrine Society, Houston, Texas, June 2012: Characterization of

Amylin Receptor and Amylin-Regulated Insulin Secretory Pathways in Rat

Insulinoma and Human Pancreatic Islet Cells. Yasemin Cagil, Saurabh

Trikha, BS and Aleksandar Jeremic, PhD.

6. American Society of Cell Biology, San Francisco, California, 2012:

Cross-Talk between Proteosomes and Lysosomes Determines the Fate of

Amyloid Peptides in Pancreatic Cells. Trikha S and Jeremic AM.

Membership:

1. Protein Society-3 years

2. American Society of Cell Biology-2 years

3. Endocrinology-1 year

4. American Diabetic Association-6 months

5. Each One Teach One Literacy Organization.

Honors and Awards:

1. 2013: Harlan Summer Research Award in biological sciences dept. at

George Washington University

2. 2012: Harlan Summer Research Award in biological sciences dept. at

George Washington University

3. 2011: Harlan Summer Research Award in biological sciences dept. at

George Washington University

4. 2008-present: Complete tuition waiver and research assistantship in

biological sciences dept. at George Washington University.

5. 2004-2008: B.Tech bachelors honor degree.

Contact this candidate