University Medical
Resume:
Kamalakkannan Srinivasan, PhD
Nashville, TN-37205
615-***-****, **********@*****.***
Post Doctoral Research Associate
Summary:
Highly motivated research professional seeking a challenging role in the area of
microbiology, protein engineering, recombinant DNA technology and cell migration.
Proven track record of groundbreaking advancement in the bacterial lipid modification of
proteins, high throughput assay development for bacterial pathogens and anti-cancer
therapeutics, directional cell migration, actin dynamics and apoptosis. Excellent
interpersonal skills with the ability to develop relationships with key opinion leaders and
multiple stakeholders across diverse scientific disciplines.
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Professional Experience:
Vanderbilt University July 2008 - Present
Post Doctoral Research Associate
Accomplishments:
Characterized spatial-temporal regulation of phosphoinositides, signaling
proteins during folate chemotaxis.
Characterized polarity and directional sensing during directed migration.
Developed high throughput assay for screening anti-cancer lead molecules
Cloning and propagation of multiple regulatory proteins in the bacterial system
Generated gene knock out mutant showing hyper polarizing phenotype and
characterized it.
Characterized phosho mutant cAR1 receptor localization and chemotaxis
Responsibilities:
Conduct independent research on cell migration.
Trouble shooting, problem solving and method development.
Collaborate with scientists from multiple institutions, coordinate and execute
cross-disciplinary projects
Train technicians, undergraduate and graduate students.
August 2006 – June 2008
University of Tennessee Health Science Centre
Post Doctoral Fellow
Accomplishments:
Elucidated molecular mechanism of protein villin mediating anti-apoptotic
function
Mapped out actin bundling sites in villin
Showed phosphorylation of villin by c-Src kinase regulates cell migration
Demonstrated inhibition of cell protrusion and retraction by Tetraspanin CD82
Cloning and propagation of fluorescent villin in bacterial system
Expression of cSrc, cYes, cFyn kinases by adeno viral infection
Responsibilities:
Developed cutting edge scientific ideas from recent literature to solve missing
knowledge on Villin’s role in actin dynamics
Organized and managed the project from inception to completion.
Assisted colleagues in other labs carry out and complete their projects
Trained graduate students.
Trouble shooting, problem solving and method development
August 2005 – July 2006
University of Kentucky
Post Doctoral fellow
Accomplishment:
Demonstrated FTase-catalyzed in vitro modification of H-Ras with FPP
analogues
Showed H-Ras modified with FPP analogues supports Rce1 and Icmt
processing.
Screened for cellular proteins modified with FPP analogues
Expression and purification of recombinant protein H-Ras from bacteria
Responsibilities:
Developed strategy and execute the projects assigned by supervisor
Set up cross-disciplinary collaborations with many different investigators.
Trouble shooting, problem solving and method development.
July 2000 – July 2005
Anna University
Graduate Student
Accomplishment:
Engineered enzyme apyrase for bacterial N-acyl-S-diacyl glyceryl cysteine lipid
modification
Constructed generic bacterial lipoprotein expression vectors.
Expression and purification of recombinant lipo-apyrase from bacteria
Demonstrated lipid modification by mobility shift and Mass spec-MALDI-TOF
Proved enhanced hydrophobicity of engineered lipo -apyrase on ELISA plate
Demonstrated enhanced immunogenicity of lipid modified protein
Optimized the screening assay for bacterial pathogens Shigella and EPEC
Responsibilities:
Reading recent literature and development of novel scientific concepts.
Exchanged novel concepts with supervisor and graduate students.
Executed the projects in consultation with supervisor.
Trouble shooting, problem solving and method development.
Taught practical course to undergraduate students.
Presented seminars on recent trends in lipoprotein engineering.
Technical Highlights:
Molecular biology
Construction of recombinant bacterial plasmids and generic expression vectors, gene
manipulation by site directed mutagenesis and gene splicing by overlap extension, gene
knock out based on homologous recombination, cDNA cloning, RT-PCR, gene silencing
by siRNA.
Protein biochemistry
Expression of recombinant proteins in bacteria using bioreactor. Purification by Affinity,
Ion exchange, Size exclusion and HIC chromatography. AKTA -FPLC and Bio Rad
systems. Identification of post translational modification by Mass spec MALDI-TOF.
Microbiology
Culturing of bacteria in shake flask and small-scale fermentor, pure culture techniques,
Gram staining, isolation of bacterial pathogens from clinical samples, Cult uring and
storage of yeast, amoeba and fungi.
Microscopy
Live and fixed cell imaging by confocal and fluorescence microscopes. FRET and TIRF
techniques.
Assays
High throughput fluorescent and imaging based anti-cancer lead assay, PCR and
colorimetric based Bacterial pathogen detection assays, Imaging based cell migration
assays.
Immunology
Immunocytochemistry, immunoprecipitation, coupling of antibodies, Western blot,
ELISA.
Virology
Infection and purification of adeno virus, Expression of proteins by adeno viral infection.
Fluorescence activated cell sorting (FACS)
Measuring and analyzing fluorescence of fixed and live cells by BD -SLR-II FACS and
Flow Jo 7.1.
Model system
Mammalian, Bacterial and Dictyostelium cells, Mouse and Rate models.
Education:
Anna University, Chennai, India.
PhD in Industrial Biotechnology – 2005
Bharathidasan University, Tiruchi, India
MS in Biochemistry – 1997
Madurai Kamaraj University, Madurai, India.
MA in Human Resource Management – 1994
Bharathiar University, Coimbatore, India.
BS in Biochemistry – 1992
Immigration status:
Permanent resident
Authorized to work for any employer
Honors & Awards:
First prize in oral presentation at Annual conference of Indian Immunology
Society, December 2004, at Anna University, Chennai, India.
Recipient of Lady Tata memorial Trust, India -Junior Research Fellowship, 2001,
August.
Associate faculty of F1000, 2012.
Associations:
American Society for Cell Biology (ASCB)-2009.
Biotech Research Society of India-2003.
Faculty of 1000, 2012-till date.
Journal Reviewer:
Molecular and Cellular Biochemistry-Elsevier publication.
International journal of Immunopathology and Pharmacology-Biolifesas.org.-
Publication
Journal biological regulators and homeostatic agents- Biolifesas.org.-Publication
Journal of Pineal Research- W iley publication.
Stem Cell discovery-Scientific research publication.
Natural Product Research-Taylor and Francis publication.
Cancer and Clinical oncology-Canadian Center of Science and Education-
publication.
Anti-Cancer drugs-Lippincot Williams & Wilkins publication.
European Journal of pharmacology-Elsevier publication.
Journal of Ethnopharmacology-Elsevier publication.
International Journal of Biology-Canadian Center of Science and Education -
publication.
International Journal of Medical Science and Public Health-publisher-APPP.
International Journal of Natural Sciences-Publisher-Sylhet agricultural university.
Editorial board:
Chief editor-Internet Journal of Physiology and Pathophysiology-Internet
Scientific Publications, LLC.
International Journal of Medical Science and Public Health-publisher-APPP.
National Journal of Physiology, Pharmacy and Pharmacology publisher -APPP.
International Journal of Biology-Canadian Center of Science and Education -
publication.
Cancer and Clinical oncology-Canadian Center of Science and Education-
publication.
International Journal of Microbiology Research-bio info publications.
International Journal of Genetics- bio info publications.
International Journal of Medical and Clinical Research- bio info publications.
International Journal of Genomics and Proteomics- bio info publications.
International Journal of Molecular Biology- bio info publications.
Publications:
Liu WM, Zhang F, Moshiach S, Zhou B, Huang C, Srinivasan K,Khurana S,
Zheng Y, Lahti JM, Zhang XA,. Tetraspanin CD82 Inhibits Protrusion and
Retraction in Cell Movement by Attenuating the Plasma Membrane -Dependent
Actin Organization.. PLoS One. 2012;7(12):e51797.Epub 2012 Dec 14.
Srinivasan K, Gus A.Wright, Nicole Hames, Max Housman, Alayna Roberts,
Karl J.Aufderheide, and Chris Janetopoulos., Delineating the Core Regulatory
Elements Critical for Directed Cell Migration by Examining Folic Acid -Mediated
Responses, - Journal of Cell Science 2012 Nov 6. [Epub ahead of prin t].
Wang Y, George SP, Srinivasan K, Patnaik S, Khurana S., Actin
Areorganisation as the molecular basis for the regulation of apoptosis in gastro
intestinal epithelial cells., Cell Death Differ., 2012, 1-11,1350-9047/12.
Mathew S,George SP,Wang Y, Siddiqui,MR, Srinivasan.K, Tan L, Khurana S.
Potential molecular mechanism for c-Src kinase mediated regulation of intestinal
cell migration, J.Biol.Chem., 2008,Vol.283, 33,22709-22.
Yaohong Wang, Srinivasan.K, Mohammad Rizwan Siddiqui, Sudeep P. George,
Alok Tomar and Seema Khurana., A novel role for villin in intestinal epithelial cell
survival and homeostasis. J.Biol.Chem., 2008,Vol.283, 14, 9454 -9464.
Sudeep P. George, Yaohong Wang, Sijo Mathew, Srinivasan.K, and Seema
Khurana., Dimerization and actin-bundling properties of villin and its role in the
assembly of epithelial cell brush borders. J. Biol. Chem., 2007, Vol. 282, 36,
Michael J. Roberts, Jerry M. Troutman, Kareem A. H. Chehade, Hyuk C.Cha,
Joseph P. Y. Kao, Xiaoqin Huang, Chang-Guo Zhan, Yuri K. Peterson,
Thangaiah Subramanian, Srinivasan.K, Douglas A. Andres, and H. Peter
Spielmann., Hydrophilic Anilinogeranyl Diphosphate Prenyl Analogues Are Ras
Function Inhibitors. Biochemistry, 2006, 45 (51), 15862 -15872.
Kamalakkannan Srinivasan, V.Murugan, K.Sankaran. Bacterial lipid
modification of proteins and its potential applications. BIO forum Europe,2005, 9,
44-46.
Kamalakkannan Srinivasan, V.Murugan, R.Nagaraj, M.V.Jagannadham,
K.Sankaran. Bacterial lipid modi ficat ion of prot e ins for nove l protein
engineering applications. Prot. Eng. Desig. & Select. 2004, 17, 721 - 729.
M.MadanBabu,Kamalakkannan Srinivasan, Y.V.B.K. Subrahmanyam and
Krishnan Sankaran., Shigella apyrase - a novel variant of bacterial acid
phosphatases? -2002, FEBS Letters, 512, 8-12.
Publications (Submitted within next 3 months):
Identification of a novel Ras Inhibitor using a simple screening approach
involving the model organism Dictyostelium discoideum., Srinivasan K,
Subramanian T, Gus Wright, Peter Spielmann and Chris Janetopoulos.
Mol.Cell.Biochem.(In preparation, will be submitted within 1 month).
Wright GA, Srinivasan K, Popatia, S, Khodadati R, Janetopoulos CJ. The
localization of the cAMP specific GPCR, cAR1, inversely mimics the cAMP
gradient during chemotaxis. Nature Communications. (In preparation, will be
submitted within 3 months).
Wright GA, Qin K, Kortholt A, Srinivasan K, Khodadati R, Van Haastert P,
Janetopoulos CJ. An RGS-containing Kinase (RckA) Modulates Rap1 activity
during cAMP chemotaxis. Molecular Biology of the Cell. (In Preparation, will be
submitted within 3 months).
Publication of conference abstracts:
Cell Surface ATP Synthase Regulates Apoptosis in Intestinal Epithelial Cells,
Gastroenterology, Volume 136, Issue 5, Supplement 1, May 2009, Pages A-758,
Yaohong Wang, Sudeep P. George, Kamalakkannan Srinivasan, Langzhu Tan,
Piyatilake Adris, Jonathan D. Kaunitz, Seema Khurana.
Reorganization of Cytoskeleton as Basis for Apoptosis Gastroenterology, Volume
134, Issue 4, Supplement 1, April 2008, Pages A-70, Yaohong Wang, Sudeep P.
George, Kamalakkannan Srinivasan, Mohammad R. Siddiqui, Sijo Mathew,
Langzhu Tan, Lester Vanmiddlesworth, Qi Xi, Jonathan H. Jaggar, Seema
Khurana.
Book chapter:
Wright GA, Srinivasan K, Janetopoulos C. Phagocytosis and Pinocytosis.
Encyclopedia of Biological Chemistry, 2nd Edition. ISBN-10: 012*******.
Publication date: March 1, 2013.
Presentation:
Poster presentation at Annual Postdoctoral Research & Shared Resources
Symposium, May 2012, Vanderbilt University-USA.
Presented poster in conference on Cell signaling and Cytoskeleton in directed
cell migration: Imaging and quantitative approaches, Joint Biotechnology and
Biological Sciences Research Council-UK and Vanderbilt University Workshop,
USA, March 4-6, 2012.
Poster presentation in Gordon Research Conference, Switzerland on June 2011.
Poster presentation in 49th annual conference of American Society for Cell
Biology at San Diego, on December 2009.
Work was poster presented at Digestive Disease Week (DDW) 2009, Chicago,
Illinois.
Poster presented at International Conference on Microbial Biotechnology:
Microcon-2009 at Punjab University, India on March 3 & 4, 2009.
Poster presentation at Digestive Disease Week (DDW) 2008, San Diego,
California.
Oral presentation 31st Annual conference of Indian Immunology Society,
December 2004, at Anna University, Chennai, India.
Poster presentation National Symposium on Developments in Biotechnology:
Emerging Trends and Challenges and First BRSI Convention (November 2004),
Conducted by North Maharastra University, Jalgaon, India.
Poster presentation in International Conference on Emerging Frontiers at the
Interface of Chemistry and Biology organized by Biotechnology Research Society
of India on April 2003 at Thiruvananthapuram.
Poster presentation in Symposium on Biotechnology at the turn of the
millennium, February 2002 conducted by Scigenics in association with Anna
university, Chennai and Madurai Kamaraj university, Madurai.
Symposium and workshop on Bioinformatics, March 2001, organized by
Bioinformatics Distributed Information Centre, Indian Institute of Science,
Bangalore.
References provided upon request.
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