Project University

CURRICULUM VITAE

Sindhu Penugonda

Tel: 704-***-****

Email: ******.*******@*****.***

Career Objective:.

Intend to build a career with leading research group in the field of biochemistry, cell and

molecular biology. My willingness to work as a key player in challenging and creative

research group makes me an asset.

Summary:

Expertise in Bio-curation, Protein expression profiling and epigenetic aspects of genes.

Experience in Protein Purification Techniques.

Electrophoresis (Horizontal and Vertical) and Western blotting.

Maintaining cells in culture, Passaging cells, Transfection of cells, Electroporation, Cell

proliferation assay, Induction of cells, Neurites counting, cell stimulation.

Isolation of DNA and RNA from biological sources.

Mouse and Rat maintenance, tagging, handling and breeding, Processing of Tissues.

Computer skills: Microsoft office, handling Altova database.

Browsing different bioinfomatics Databases like PubMed, MeSH, Merops, Chem ID

plus, Brenda, KEGG, MGI, Biocarta, Source, IHOP etc..

Tools- BLAST, SMART, CLUSTALW, BLASTp.

Basic microbiological techniques like inoculation, Sterilization, serial dilution

methodology and staining techniques.

Educational qualifications:

Education Institute University/ Board Year of

passing

Research in Dept of Molecular biology, Umea University 2010

Molecularbiology Sweden

M.Sc Biochemistry Manasagangotri, Mysore. University of 2007

Mysore

B.Sc Maharani’s Science college University of 2005

Biochemistry, for women, Mysore. Mysore

Botany

Microbiology

Achievements: Ranked 5th in the University during M.Sc.

Class topper in B.Sc

Subjects studied during M.Sc:

Biochemical techniques, Biotechnology, Molecular biology, Cell biology, Immunology,

Biostatistics, Bioinformatics and Computers, Enzymology, Genetics, Clinical

biochemistry, Physiology and Nutrition, Biomolecules and Metabolism. Biophysical

chemistry, Bio-organic chemistry and Microbiology.

Research Experience: 4 years

1) Worked as research student under the guidance of Prof. Bengt Hallberg,

Department of Molecular biology, Umea University, Sweden.

Worked on Investigation of function of anaplastic lymphoma kinase (ALK) and its

downstream signaling, mutants of ALK gene have been identified in neuroblastoma

patients, the occurrence of these mutations are noted and transiently transfected mutants

are constructed in different cell lines. These cell line models have been studied for

checking the characteristic futures of neurite formation; effect of mutations on

proliferation, cell signaling and downstream events of ALK activation using some

inhibitors is also studied. ALK expression patterns and its signaling analyzed using

western blot, ECL development and chemiluminescence.

Credits received: 30

Duration: 9 months (Sep 2009- Jun 2010)

2) Teaching experience:

Worked as a chemistry lab lecturer in Mount Carmel Degree College, Bangalore.

Duration: 6 months (Feb 2009 - Jul 2009)

3) Work experience:

Worked as a Junior Research Associate for Molecular Connections,

Basavanagudi, Bangalore, India.

Mining of literature to construct signaling cascades with genes, proteins and its

interacting small molecules, this data was compiled to build a scientific database.

Expertise in Bio-curation, Protein expression profiling and epigenetic aspects of

genes.Worked on Literature informatics aspects of proteases and protein substrates.

Excellent in understanding biological research papers, reviews and journals. Experience

in 3 different projects related with Diabetes and Cancer and have had many opportunities

to guide many of my juniors.

Duration: 1 year 7 months (Jun 2007 - jan 2009).

4) Project title: “Oxidative modification of high density lipoprotein associated

Paraxonase: Role of water and fat soluble antioxidants” under the guidance of

Prof.Cletus.J.M.D.Souza, Department of studies in Biochemistry, Manasagangotri,

University of Mysore, Mysore

Paraoxanase enzyme was obtained from human serum and purified using cibacron gel, its

activity was tested by PON assays spectrophotometrically by time kinetics. As this

enzyme is associated with HDL we tested its oxidation using H2O2, lipid peroxides and

copper catalyst. Further the Oxidation of PON by H 2O2 and copper was inhibited by

water soluble antioxidants like ascorbic acid and analog of curcumin, and lipid peroxide

caused damage of PON was recovered by fat soluble antioxidant curcumin.

We derviatized Curcumin by attaching glycine this became water soluble and was an

antioxidant to water soluble oxidants like Cu++ and H2O2.

Duration: 6 months (Nov 2006 - May 2007)

5) I worked as a Research student on “Action of garlic on lipid peroxidation and

antioxidation enzymes seen in the animal models having skin carcinoma induced by

7,12-dimethylbenz[a]anthracene”. In Department of studies in Biochemistry,

University of Mysore, Mysore, Karnataka, India.

I worked on the action of dietary phytochemicals such as garlic having cancer

protective effects, we introduced skin carcinoma by 7,12-dimethylbenz[a]anthracene in

swiss albino mice model, pre and post garlic treatments where given to different groups

of mice and now we are investigating the histology of the affected skin. Along with that

we performed biochemical assays using the liver tissues of mice, for lipid peroxide by

TBA assay, catalase, superoxide dismutase by pyrogallol auto-oxidation and glutathione

peroxidase spectrophotometrically which demonstrate the effect of garlic in mice. Further

results obtained, proves that the Garlic induces elevation in levels of antioxidants, which

was accompanied by decreased lipid peroxidation and incidences of papillomas, thus

protecting cells from injury by the oxidized molecules.

Duration: 3 months (Jul 2006- Sep 2007)

6) Project title: “Over expression and purification of GST-RdRp” under the guidance

of Prof. H.S. Savithri, Dept of Biochemistry, Indian Institute of Science (IISc),

Bangalore.

The RdRp clones 52GST RdRp,62GST RdRp,52CΔ43GST RdRp and 62CΔ43GST

RdRp were transformed into BL21 Rill, BL21 Rosetta (DE3),BL21 pLys S cells and BL21

C43(DE3) competent cells incubated and heat shocked for 5 minutes and later plated on

LB-amp plate, positive cultures were obtained in large quantities using LB amp broth

(Lauryl tryptose broth). Culture was induced with IPTG and incubated, sonicated,

centrifuged, supernatant put for GST binding and protein eluted using GST elution buffer.

The eluted protein samples were loaded on SDS-PAGE and western blotting was done.

Protein was further dialyzed and used for characterization purpose.

Duration: 3 months (Summer project 2006)

Techniques learnt during projects: Cell culture, Cell proliferation assay, Nucleofection,

Restriction digestion of plasmid DNA, Affinity column, Gel elution, Protein Extraction,

Protein expression checking, SDS PAGE, GEL documentation, Western blotting, PON

assays, Spectrophotometer, Centrifugation, Sonication and bacterial transformation.

Skillsets:

Technique skills:

1) Analytical and separation techniques:

Chromatography – Circular and 2D Paper Chromatography, TLC, Gel filtration, Ion

Exchange, Affinity Chromatography.

Electrophoresis –SDS PAGE, PAGE-acidic and basic.

Centrifugation.

Soxhlet Extraction.

Homogenization.

Dialysis.

2) Microbial techniques:

Sterilization of glassware’s.

Preparation of media.

Isolation from soil, water, food, etc.

Serial dilution.

IMVIC test.

Colony characterization-Classification of microbes based on shape, cell wall, etc.

Inoculation by Pour plate, Spread plate, Zigzag streak, Parallel streak methods,etc.

Staining – Gram staining.

Acid fast staining.

3) Enzymological techniques:

Extraction, Purification and characterization of enzymes.

Kinetic assays and End point assays of various parameters such as Time, Substrate,

pΗ for enzymes like Invertase, Salivary Amylase,etc.

4) Immunological techniques:

Single and Double radial immunodiffusion.

ELISA.

5) Genetics and Molecular Biology:

Identification of drosophila mutants, single mutant crosses.

Isolation of DNA and RNA from biological sources.

Quantitative estimation of DNA and RNA.

6) Clinical Biochemistry:

Urine analysis (qualitative, quantitative).

Blood analysis (qualitative, quantitative).

Techniques known:

1. Tissue culture: Maintaining cells in culture, Passaging cells, Transfection of cells

using Lipofectamine, Electroporation using Amaxa nucleofector system, Cell

proliferation assay, Doxycyclin induction of cells, Neurites counting, cell

stimulation using antibody.

2. Enzyme assay: Extraction, Purification and characterization of enzymes.

Kinetic assays and End point assays of various parameters such as Time,

Substrate, pΗ for enzymes like Invertase, Salivary Amylase,etc.

3. Acid phosphatase, alpha-amylase, Salivary amylase, Invertase: Activity and specific

activity, Temperature, Time and pH optimums, Substrate

kinetics, kinetic assay.

4. Molecular biology: DNA, RNA isolation and estimation, agarose gel

electrophoresis, Plasmid isolation, Western Blotting, Yeast cell permeablisation,

ECL development.

5. Biochemical techniques: Paper (circular, ascending and descending), TLC,

Column (gel) Chromatography techniques, Soxhlet extraction, Colorimetric and

UV-Spectrophotometric estimations, Ultra centrifugation, Dialysis, Fractional

distillation, Gel documentation, Chemiluminescence.

6. Molecular biology techniques: Extraction of plasmid, phage and genomic DNA,

Quantification of DNA by spectroscopic and Agarose gel electrophoresis, gel

elution, Ligation, Conjugation, Isolation of total RNA from yeast, Western Blot,

SDS PAGE, X β-gal screening and MacConkey screening, excision of phage

DNA.

7. Animal Experiments: Mouse and Rat maintenance, tagging, handling and

breeding, Processing of Tissues.

Computer skills:

Microsoft office: MS word, Power point, Excel.

Database: Altova

Bioinformatics: Browsing different bioinfomatics Databases.

Tools- BLAST, SMART, CLUSTALW, BLASTp.

Seminars presented: Discovery of Vitamin C

Ribozymes

Cytokines

Other achievements: Athletics individual championship

Women’s Chess championship

Women’s double Carom championship

Recognition as Best Curator in Curex and Protease projects

Hobbies: Snorkeling, Kayaking, Swimming, Biking, Reading, Dancing, embroidering

Languages known: English, Hindi, Kannada, Telugu and Sanskrit.

Career highlights: Distinction in B.Sc and all 4 semesters of M.Sc from University of

Mysore, Mysore.

Strengths:

A good team player with leadership quality.

Strong conceptual, logical and presentation skill.

Good inter-personal communicational skills.

References:

Prof. Bengt Hallberg,

Department of Molecularbiology,

Umea University,

Sweden

Ph: +46-90-785****

E-mail: *****.********@*******.***.**

Prof.Cletus.J.M.D’Souza,

Department of studies in Biochemistry,

Manasagangotri,

University of Mysore,

Mysore

Ph: +91-944*******

E-mail: *********@*****.***

Prof.Karunakumar M K,

Department of studies in Biochemistry,

Manasagangotri,

University of Mysore, Mysore

Ph: +91-988*******

E-mail: *********@**********.***

Contact this candidate