University Project

CURRICULUM VITAE

MADHURIMA DAS

E-mail: ******@*****.*** 5200

Anthony Wayne Drive,

Phone no: 313-566-34380429

Apartment No. 603,

Detroit, MI-48202

SKILLS:

. Agarose Gel Electrophoresis

. PCR

. Restriction digestion

. HD cloning

. TOPO Cloning

. TIRF microscopy

. Mammalian Cell Culture

. SDS PAGE

. Western Blotting

. Contig Annotation

. Agarose Gel Electrophoresis

. HD cloning

. Restriction digestion

. TOPO Cloning

. Teaching

PROJECTS

1) Molecular Biology: Study of Expandase Gene, Orchid Chemicals and

Pharmaceuticals (2nd-17th June, 2009)

Description: Cloning of expandase gene from Streptomyces clavuligerus into

DH5alpha strain of E. coli.

2) Molecular Biology: Expression of has operon genes in Lactococcus

lactis, IIT Madras (23rd November- 19th December, 2009)

Description: Detection of enzymes produced by has operon genes in

genetically modified Lactcoccus lactii.

3) Bioinformatics: Whole Genome Transcriptome Sequenceing Project

involving Jatropha curcas, Department of Genetic Engineering, SRM

University, Kattankulathur

Description: Annotation of 1000 contigs obtained from sequencing of

Jatropha curcas.

4) Microbiology: Degradation of Phenanthrene by a Bacterial Strain

Present in Petrol Contaminated Soil, IIT Madras (24th May- 2nd July,

2010)

Description: Isolation of bacteria that can grow on media containing

pollutant Phenanthrene as the sole carbon source.

5) Oncology: Studying the Effects of Specific Monoclonal Antibody on HER2

Overexpressing Cell Line, Biocon Ltd., Bangalore (3rd January, 2011-

3rd April, 2011)

Description: Determining the effect of an anti-breast cancer antibody on

BT474 cell lines. My undergraduate thesis was based on this project.

6) Fly work:The use of UAS/GAL4 system to introduce SIN3 knockdown in

Drosophila melanogaster salivary glands

Description: Studying the effect of the UAS/GAL4 system on Drosophila

melanogaster survival and its efficacy as a system to produce populations

of Drosophila with SIN3

Knock down.

7) TIRF Microscopy: Using GFP tagged C-peptide to study Insulin Secretion

in Min6 Cells:

Description: Studying the role of c-peptide in exocytosis of insulin-

containing vesicles via TIRF microscopy in Min6 insulinoma cells.

8) Sub-cloning: Studying the protein-protein interactions of

Synaptotagmins 1 and 7:

Description: Sub-cloning of Synaptotagmins 1 and 7 fused to Bir A in order

to study their protein-protein interaction by the BioID method.

EDUCATION QUALIFICATION

DEGREE BOARD/ INSTITUTION YEAR %

UNIVERSITY

10th std CBSE ST. JOHN'S ENGLISH SCHOOL 2005 94%

AND JUNIOUR COLLEGE

AN AND JUNIOUR

12th std CBSE ST. JOHN'S ENGLISH SCHOOL 2007 84 %

AND JUNIOUR COLLEGE

B. TECH (GENETIC SRM UNIVERSITY SRM COLLEGE OF ENGINEERING, 2011 8.562 on a

ENGINEERING) SRM UNIVERSITY scale of 10

(semester

1-8)

3.57 on a

MS (BIOLOGICAL WAYNE STATE UNIVERSITY 2011- scale of 4

SCIENCES) July (Up to

2013 Semester 2)

CO-CURRICULAR ACTIVITIES AND ACHIEVEMENTS

POSTER PRESENTATION

1. "Degradation of Phenanthrene by Bacterial Strain Present in Petrol

Contaminated Soil" at the International Conference on Bioengineering,

School of Bioengineering, SRM University, Kattankulathur

2. "Studying the Effects of Specific Monoclonal Antibody on HER2

Overexpressing Cell Line" at SRM University

CONFERENCES ATTENDED

1) National Conference on Recent Advances in Bioengineering, School of

Bioengineering, SRM University, Kattankulathur

2) Stem cells, Regenerative Medicine in Orthopaedic Applications,

Department of Biomedical Sciences, Sri Ramchandra University,

Chennai

3) International Conference on Bioengineering, School of

Bioengineering, SRM University, Kattankulathur

TEACHING EXPERIENCE:

1. Microbiology (BIO2200)lab at the Department of Biological Sciences,

Wayne State University- Fall 2011

2. Basic Life Mechanism (BIO1510) lab at the Department of Biological

Sciences, Wayne State University- Winter 2012

3. Basic Life Mechanism (BIO1510) lab at the Department of Biological

Sciences, Wayne State University- Spring-Summer 2012

4. Basic Life Mechanism (BIO1510) lab at the Department of Biological

Sciences, Wayne State University- Fall 2012

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