Kimberly Ann Hoyt, M.S.
********@*****.***
San Diego, Ca 92109
Profile
Biologist with experience in the practical application of biochemistry and molecular biology techniques is seeking Research Associate position.
• Extensive experience with MS office, spreadsheets, data entry, data analysis, computation, analytical processes, statistical analysis, data graphics program, and problem solving.
• Background in biochemistry, molecular biology, and protein laboratory techniques.
• Ability to work interactively with multi functional disciplinary teams as well as independently.
• Detailed oriented and good record keeping skills.
• Adherence to SOPs/universal precaution guidelines and GMP practices.
• Working knowledge of laboratory methods, clean laboratory skills, preparations of reagents, buffer solutions, laboratory safety, hazardous material procedure, and regulatory practices.
• Experience in flow cytometry, organizing, assembling, and implementing comprehensive laboratory equipment.
• Successful training of undergraduate science majors in biochemistry laboratory techniques.
Methodology and Skill set
Molecular – FacScan Flow Cytometry, Apoptotic DNA analysis techniques, Bacteria plasmid vector, PCR, agarose electrophoresis, bacteria identification.
Biochemistry – Bioassays, cell culture, growth media preparation, protein purification, spectrometer, SDS-Page, autoclaving.
Other – Trace metal analysis, protocols and method development for trace metals analysis in various samples (ICP-OES, ICP-MS), mercury/monomethylmercury analysis following US-EPA method (CV-AFS).
Experience
Environmental Consultant Environmental Inspector / Biological monitor March 2011 – Present
Chambers Group Inc. 9909 Huennekens St., San Diego
• Conducting biological surveys, habitat assessments, and focused surveys for federal- and state-listed species and California species of special concern.
• Working knowledge of southern California biota, and have conducted focused surveys for sensitive plant species in San Diego County.
• Have performed environmental reviews, monitoring for biological resources, biological and executive summary reports
Biotechnology Firm Lab Support – On Assignment January 2011 – March 2011
Sayrna Technologies 6990 Carroll Rd., Ste A
• Operation of Flow Cytometer measuring enzyme activity and inhibition with various chemical assays.
• GMP Practices.
• Tecan FreedomEvo robotic platform workstation.
Biotechnology Firm Laboratory Technician February 2010 – November 2010
Phoenix Flow Systems 6790 Top Gun St #1, San Diego
• Development of bioassays protocol used for testing of enzymatic activity.
• Assembly of TUNEL assays kits and reagents kits.
• Implemented bioassays for measuring the stability of control cells.
• Operation of Flow Cytometer FacScan, in the measurement of cells in a flow system.
• Adhere to SOPs/universal precaution guidelines.
• Quality control by in process and final product testing.
• Created reports/summaries/protocols. Generate appropriate audit trail for all activities.
• Microbiology lab assistant/technician. Maintain laboratory equipment/inventory levels.
• Prepare or preserve buffers/reagents.
• Assembly and implementation of comprehensive manufacturing and production equipment.
• Ability to perform cleaning and sterilization of manufacturing/production equipment.
Graduate Student (laboratory of Michael Latz) 2007- 2009
University of California, San Diego
• Discovered that particle size interactions with substances is one of the main factors influencing bioavailability to plant tissue.
• Investigated particle characteristics to size and organic content, dissolved ion substances forms, kinetic rate of tissue uptake, and affect on various plant tissues in bioavailability of substances.
• Established two fold experimental design to determine environmental factors affecting contaminant accumulation and kinetic rate of uptake in plant tissues.
• Implemented induced coupled plasma mass spectrometer and cold vapor fluorescence mass spectrometer for measuring substance content levels in tissue and sediment samples.
• Developed protocol for nitric acid digestion of plant tissues and sediment for the analysis of substances.
• Obtained funding through Grant writing for research
• Demonstrated organizational, communication, data analysis, presentation, and report writing.
Teaching Assistant
University of California, San Diego $1,800.00 month- part time work (20 hr wk)
Biochemistry Lab Techniques March-June 2009
Cell Biology March-June 2008
Structural Biochemistry September-December 2007
Undergraduate (laboratory of Doug Bartlett) January 2006 - February 2007
University of California, San Diego
• Implemented molecular biology techniques.
• Determined bacterial content of Venice Lagoon samples.
REU Research Experience for Undergraduate (Roland Lamberson’s Lab) June - August 2005
Humboldt State University
Education
Quality Assurance and Control Certificate Program January 2010 – Current (online courses)
UCSD Extensions Life Sciences
M.S. Biology Graduated September 2009
University of California, San Diego GPA – 3.91
Advisor: Deheyn D. Dimitri
Thesis title: Levels of Metals from Salt Marsh Plants from Southern California, USA
B.S. Biochemistry and Cell Biology Graduated June 2007
University of California, San Diego GPA – 3.65