Engineer Pharmaceutical

SUMMARY

Ph.D, Pharmaceutical Science

Solid background in pre-formulation, formulation, analytical

chemistry, drug delivery, drug metabolism, bio-analytical

characterization, nanotechnology and pharmacokinetic analysis

EDUCATION

Ph.D, Pharmaceutical Science Rutgers University, New Brunswick, NJ,

October 2012

B.S., Environmental Science & Engineering Tunghai University,

Taichung, Taiwan, July 2002

PROFESSIONAL SKILLS

Research Systems

Chromatography: HPLC, SEC-HPLC, UPLC, gel permeation chromatography

(GPC), silica gel purification;

Mass Spectrometry: LC-MS/MS, MALDI-TOF/MS, ESI-MS; Microscopy:

fluorescence microscopy, confocal microscopy; Spectroscopy: UV/Vis,

NMR, DLS

Synthetic Skills

Peptide/nanocarrier/nanoparticle synthesis, click chemistry,

PEGylation, lyophilization, glycosylation, pre-formulation, pro-drug

conjugation, stability characterization, hydrogel preparation

Biological techniques

Tissue culture, cellular uptake studies, membrane receptors induction,

receptor binding affinity, in vitro/ in vivo studies, mammalian tissue

culture, animal handling/managing, primary rat peritoneal cells

isolation and polarization

Bio-analytical characterization

Western-blotting, ELISA, RT-PCR, Gel electrophoresis, SDS-PAGE,

Bradford protein assay, immunoassays, heterotypic cells co-cultured

interaction, drug release study, MTT assay, fluorescence-activated

cell sorting (FACS)

Computer

Photoshop, LAS AF Lite (Confocal), Waters Empower system, Graphpad

Prism, Microsoft Office, Windows, Mac OS

EXPERIENCE

Rutgers University Department of Pharmaceutical Science, Since

October 2012

Postdoctoral Fellow

. Design, synthesize and characterize the spacer flexibility effect at

mannosylated ligand in mannose receptor mediated uptake

Rutgers University Department of Pharmaceutical Science, September

2006-October 2012

Ph.D Research/Graduate Assistant

Dissertation: Macrophage Targeting Using Nanotechnology for Anti-HIV

Therapy

. Conduct research in drug targeting and delivery for HIV therapy

using nanotechnology

. Design & synthesis of biodegradable PEGylated nanocarriers (NCs) and

multivalent mannose PEGylated NCs

. Evaluate mannose receptor mediated uptake of NCs into polarized rat

peritoneal macrophages in vitro/ in vivo

. Evaluate anti-HIV activities of drug conjugated NCs in HIV-1

infected human monocyte derived macrophages

Schering Plough Research Institute DMPK, Exploratory Drug Metabolism

Department, summer 2009

Pharmaceutical Industry Intern

. Assessed hepatotoxicity mechanism of parent drugs and their

metabolites using HepG2 and cultured cryopreserved primary human

hepatocytes

. Enhanced the hepatocyte function of dog and mice hepatocytes through

heterotypic cell-cell interaction

. Predicted the in vivo hepatic clearance of marketed model compounds

using a microfluidic in vitro culture system

Wyeth Pharmaceuticals Formulation Group, summer 2005

Pharmaceutical Industry Intern

. Determined the solubility of commercially available drug-like

compounds and nine hydrophobic Wyeth compounds, including acids,

bases and neutral compounds, using HPLC

Environmental Engineer BeiTou Incinerator, Dept. of Environmental

Protection, Taipei City Government,

Environmental Engineering March. 2002 - March. 2004

. Optimized the humidity of pre-boiled sewage for energy efficiency and

pollution control

LEADERSHIP

President Taiwan Study Association (TSA), Rutgers University,

January 2008 - January 2009

Vice President Graduate Research Association of Students in Pharmacy

(GRASP), July 2006 - July 2007

Treasurer Graduate Student Association of Pharmaceutical Science,

September 2007 - May 2008

PUBLICATIONS

Multimeric Peptide-based PEG Nanocarriers with Programmable

Elimination Properties

Biomaterial, 30(29): 5649-59, 2009

. Gunaseelan S1, Pooyan S1, Chen P, Samizadeh M, Stein S, Zhang X, Sinko

PJ.

Novel monodisperse PEGtide dendrons: design, fabrication and

evaluation of mannose receptor-mediated macrophage targeting

Bioconjugate Chemistry, 2013 (Submitted)

. Gao J, Chen P, Singh Y, Zhang X, and Sinko PJ.

Structural Optimization of Mannosylated Nanocarriers for Macrophage

Targeting

Journal of Controlled Release, 2013 (Submitted)

. Chen P, Szekely Z, Zhang X, Sinko PJ.

Design of HIV Inhibitors Conjugated Nanocarriers for Macrophage

Mannose Receptor Targeting and Evaluate Their Anti-HIV Activities,

2013 (To Be Submitted)

. Chen P, Zhang X, Szekely Z, Sinko PJ.

Polarized Macrophage (M1, M2 phenotypes) Targeting and Uptake Using

Mannosylated Nanocarriers via Mannose Receptor, 2013 (To Be Submitted)

. Chen P, Zhang X, Szekely Z, Sinko PJ.

HONORS AND AWARDS

. Fellowship of National Institute of Health grant number R01A84137

2010-2012

. Scholarship of the Professor Chen Wen-Chen Memorial Foundation

2010

. Parke-Davis Chair Fellowship 2008- 2009

. Fellowship of Westward Pharmaceuticals grant fund 2007-2008

. Fellowship of National Institute of Health grant number AI 51214

2006-2007

. Rutgers, travel award of American Association of Pharmaceutical

Scientist (AAPS) 2007, 2008, 2010

SEMINAR & POSTER PRESENTATIONS

Poster presentation American Association of Pharmaceutical

Scientists (AAPS), Chicago, IL 2012

"Architectural Optimization of Mannosylated Nanocarriers for

Macrophage Targeting"

Poster presentation American Association of Pharmaceutical Scientists

(AAPS), Washington DC, 2011

"Targeting the Macrophage Mannose Receptor (MR) on J774-E Cells Using

Multivalent Mannosylated PEG Nanocarriers"

Poster presentation American Association of Pharmaceutical Scientists

(AAPS), Los Angeles, CA 2009

"Multimeric peptide-based PEG nanocarriers with programmable

elimination properties"

"Targeting Murine Macrophage Cell Line Using Multivalent Mannosylated

Peptide Backbone PEG Nanocarriers"

Poster presentation Society of Toxicology (SOT) Annual Meeting,

Baltimore, MD 2009

"Macrophage Targeted Multivalent Mannosylated Peptide Backbone PEG

Nanocarriers:Uptake into J774-E Cells."

"Versatile and Biodegradable PEGylated Peptide-based Novel

Nanocarriers"

Poster presentation American Association of Pharmaceutical Scientists

(AAPS), Atlanta, GA 2008

"Macrophage Targeted Multivalent Mannosylated Peptide Backbone PEG

Nanocarriers: Uptake into J774-E cells."

Contact this candidate