Assistant High School

SUMREET SINGH JOHAR

G-** First Floor, Street No.*1, Hari Nagar, New Delhi – 110058, India

919*********, ************@*****.***

EDUCATION

STATE UNIVERSITY OF NEW YORK AT STONY BROOK, NEW YORK

Master of Arts in Biology, December 2012, GPA: 3.65/4.00

UNIVERSITY OF DELHI SOUTH CAMPUS, INDIA

Master of Science in Biochemistry, June 2009, Aggregate percentage : 72.10

SRI VENKATESWARA COLLEGE, UNIVERSITY OF DELHI, INDIA

Bachelor of Science in Biochemistry, June 2007, Aggregate percentage : 84.12

TEACHING EXPERIENCE

Teaching Assistant in the Department of Undergraduate Biology, State University of New York at Stony Brook.

Spring 2010

Lecture Teaching Assistant: Assisted professors in teaching the recitation class of BIO 361 Course: Biochemistry Part I

by proctoring exams, conducting office hours and grading the exam papers.

Fall 2010

Laboratory Teaching Assistant: Supervised and instructed undergraduate students of lab BIO 311 Course: Techniques

in Molecular and Cell Biology, graded lab reports, conducted office hours, prepared exam questions and final grade

reports.

Fall 2012

Laboratory Teaching Assistant: Supervised and instructed undergraduate students of lab BIO 204 Course:

Fundamentals of Scientific Inquiry in Biological Sciences, graded lab reports, conducted office hours and prepared final

grade reports.

Instructed and supervised an undergraduate student for the cloning of Epidermal Growth Factor like Repeats in bacterial vector.

LABORATORY EXPERIENCE

STATE UNIVERSITY OF NEW YORK AT STONY BROOK, NEW YORK

Graduate Research Assistant, Summer 2010 – December 2012 (Supervisor: Professor Robert S. Haltiwanger)

Projects

Cloning and large scale purification of glycosyltransferases: mouse Pofut1 and mouse Rumi from HEK 293T cells.

Cloning and purification of Drosophila Rumi, a glycosyltransferase for X-ray studies.

Effect of protein-O-fucosyltransferase 2 (Pofut2) on the folding of Thrombospondin Type 1 Repeats (TSRs).

Lab Rotation Projects

January 2010 – March 2010 (Supervisor: Professor W. Todd Miller)

Expression of Srm, a non receptor tyrosine kinase in Sf9 insect cells .

Regulation of Srm, a non receptor tyrosine kinase by Brk in SYF cells.

September 2009 – November 2009 (Supervisor: Professor Richard R. Kew)

Analysis of interaction of a complement protein receptor, gC1qR with Vitamin D Binding Prot ein in HL60 and U937 cell

lines.

UNIVERSITY OF DELHI SOUTH CAMPUS, INDIA

Projects

August 2008 – March 2009 (Supervisor: Professor Alo Nag)

Regulation of HPV E6-Interacting Transcriptional Co-activator Human ADA3 by SUMOylation.

May 2008 – July 2008 (Supervisor: Professor Debi P. Sarkar)

Characterization of Membrane Fusion Potential of Sendai Virus .

LABORATORY SKILLS

TECHNIQUES

Molecular Biology: Transformation, Polymerase Chain Reaction (PCR), Chromosomal DNA Isolation from Human

Blood, Plasmid Purification, Restriction enzyme digestion, DNA ligation, DNA sequence analysis, Agarose Gel

Electrophoresis, Site Directed Mutagenesis, Primer designing, Molecular c loning.

Biochemistry: SDS PAGE, Western Blot, ELISA, Immunoassays, High Performance Liquid Chromatography (HPLC),

Protein Dialysis, Lyophilization, Protein purification from bacterial, insect and mammalian systems using affinity

chromatography, Gel filtration Chromatography, Ion-Exchange Chromatography, Spectrophotometry, Radioactive

assays for glycosyltransferases, Immunoprecipitation, Mass Spectrometry (LC-MS/MS), Flow Cytometry, pH meter.

Tissue Culture: Transient transfection in 293T cells, HeLa cells, SYF cells, Lec13 CHO cells, Baculovirus Sf9 cells,

and drosophila S2 cells, Generation of stable S2 cell lines.

COMPUTER SKILLS: Microsoft Office (Word, Powerpoint, Excel), BLAST, CLUSTAL W, Expasy, Oligo Analyser, EndNote, Sequencher,

Blackboard Instruction Technology, Clicker Instruction Technology.

LANGUAGE SKILLS: Fluency in English, Hindi and Punjabi.

CO-CURRICULAR ACTIVITIES

Participated in a Seminar on Modern Biology (Topic - “Laws of Intracellular Transport: Motors, Tracks and Traffic Jams”) in 2008

organized by Department of Biological Sciences, Tata Institute of Fundamental Research (TIFR), Mumbai, India.

Presented a Poster on “Regulation of HPV E6 – Interacting Transcriptional Co-activator Human ADA3 by SUMOylation” in the

International Symposium on “Novel Strategies for Targeted Prevention and Treatment of Cancer,” held at School of Life

Sciences, Jawaharlal Nehru University, New Delhi, India on 19-20 December, 2008.

Participated as a judge in the Science Fair for High School Students in 2011 at Syosset High School, Syosset, New York.

Presented a Poster on “Study of Oxidative Folding of Thrombospondin Type 1 Repeats” at the MCB and BSB Training Grant

Retreat held at Charles B. Wang Center, Stony Brook University, New York, USA in September 2012.

Presented a Poster on “Analysis of Effect of Protein O-fucosyltransferase 2 (Pofut2) on the Oxidative Folding of Thrombospondin

Type 1 Repeats” at the Institute of Chemical Biology and Drug Discovery Sixth Annual Symposium held at Charles B. W ang

Center, Stony Brook University, New York, USA in October 2012.

HONORS & AWARDS

Qualified Graduate Aptitude Test in Engineering (GATE) 2008 and 2009 in Life Sciences, an all -India examination administered

and conducted jointly by the Indian Institute of Science and seven Indian Institutes of Technology, with 97.19 and 98.94

percentile respectively.

Selected for Junior Research Fellowship in the National Eligibility Test conducted jointly by Council of Scientific and Industrial

Research and University Grants Commission, India in June 2008.

Scored 650 out of 990 (89 percentile) in Biochemistry, Cell and Molecular Biology Subject Graduate Rec ord Examination

(Biochemistry: 94 percentile, Cell Biology: 88 percentile and Molecular Biology & Genetics: 74 percentile).

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