Project Training

CURRICULUM VITAE

NEVIL SRIVASTAVA

Home Address:-

D/o, Shri Viresh Kumar Srivastava Phone:+91-895*******,

B-66 Sector-1, Door Bhash Nager +91-945*******

Raebareli-229010 E-mail:**********.*****@*****.**

Personal Profile:-

Name Nevil Srivastava

Father’s Name Shri Viresh Kumar Srivastava

Mother’s Name Smt Usha Srivastava

Nationality Indian

Marital Status Unmarried

Date Of Birth 03-05-1987

Educational Qualification:-

Degree Institute/University Year Majors Division/

percentage

Ist /69%

MSc Bundelkhand university 2011 Biotechnology

Biotechnology Jhansi

I st /72%

BSc Bundelkhand university 2009 Biotechnology

Biotechnology Jhansi

(Hons)

Ist /70%

12th UP Board 2004 Biology

Ist/74%

10th UP Board 2002 Science

&others

Research work Experience:-

One year ( 04-05-2012 to 30-03-2013) Senior Research Fellow entitled “ICAR funded

outreach project Diagnosis And Management Of Leaf Spot Disease Of Field And

Horticultural Crops” under supervision of Mrs Sunita Lal (Principal Scientist) at crop

protection division,Indian Institute of Sugarcane Research (IISR) Lucknow .

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Summary of the project work:- Random Amplified Polymorphic DNA (RAPD) procedure was

used to examine the genetic variability of C.falcatum. The data were entered into a binary matrix

and a similarity matrix was constructed using DICE similarity (SD) index. A UPGMA cluster

based on SD values was generated. FreeTree software was used for construction of phylogenetic

trees on the basis of distance data and for bootstrap analysis of the trees robustness.

M.Sc dissertation work:-

Four months Dissertation (10th Jan. 2011-9th May 2011) entitled “Cloning expression and

purification of Brugia- malayi Endosymbiontgene (Bme)” for the partial fulfillment of M.Sc

Bio Tech, under the guidance of Dr. (Mrs.) Shailja Bhattacharya Scientist ‘G’ Parasitology

Division C.D.R.I Lucknow (UP)

Summary of the dissertation work:-Previous strategies for elimination of filariasis have

included vector control in the presence of antiparasitic drugs. Diethylcarbamazine, and

ivermectin have been the most recent drugs of choice for prevention of microfilarial infections,

but they have little effect on adult worms. It is thought that Wolbachia may be used as a future

candidate against lymphatic Filariasis.The recent publication of the draft genomic sequence for

Wolbachia enables us a genome wide search for new potential drug targets .The aim of the

present present study is to make the platform for identifying the drug target, the protein(s) of

Wolbachia by gene targeted reverse transcription polymerase chain reaction, its cloning,

subcloning and further its expression and purification. A protein thus identified would further be

characterized. The role of identified protein(s) in parasite-host biology would be explored or it

may be exploited as a vaccine/drug target.

Training Experience:-

One month training from Biotechnology and Molecular Biology Centre, Mahatma Gandhi P.G.

College, Gorakhpur.

One month training from Parag Co-operative Milk Union Ltd., Lucknow

Technical Proficiency:-

Molecular biology:-

Culturing of Microorganisms, Isolation and Enumeration of microorganism, Staining Methods,

Tissue Culture Techniques,Gel Electrophoreses(SDS PAGE,Agarose), DNA Isolation from plant

tissues/microorganisms/animal tissues, Plasmid Isolation, Bacterial transformation, Vector

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Maintanance, Chromatography (Thin layer, Paper and Affinity chromagography),PCR,Western

Blotting,Protein Purification,ELISA, Genetic diversity analysis by RAPD marker.

Animal Handling:-

Experience in handling mice,Intra Muscular (IM) and Intra Peritoneal (IP) injections, Isolation

of body organs like spleen liver, etc.,

Computer and bioinformatics:-

One year Computer foundation Course from Bundelkhand University,jhansi.

Conferences:-

Participated in National Seminar on Recent Advances and New Inclinations in Biological

Sciences(NSRANIBS-2012)April 18th at Department of Biological sciences, SHIATS(Formerly

Allahabad Agriculture Institute), Allahabad, U.P

References:-

Mrs Sunita Lal(Principal Scientist) Dr.(Mrs) Shailja Bhattacharya (Scientist G,

Crop Protection Division Parasitology Division

Indian Institute of Sugarcane Research, Lko Central Drug Research Institute, Lko

Email:- ****.****@*****.*** Email:-********************@****.***.**

Dr. P.K Pandey (Reader& Head)

Biotech Department

Bundelkhand University, Jhansi

Declaration:

I, hereby, declare that all the information provided is true to the best of my knowledge.

DATE : 16-04-2013

PLACE : RAE BARELI (NEVIL SRIVASTAVA)

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