Project Technician

BIOGRAPHICAL SKETCH

NAME POSITION TITLE

Aditi K Kaushik, MSc. Graduate Student

Texas A&M University,

College Station, Texas

Address: Texas A&M University

*** ***** #*

College Station, Texas - 77840

Ph: 510-***-****

Email: **********@*****.***

EDUCATION/TRAINING

INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY

DEGREE, TITLE

Amity University, Delhi, India Bachelor of 2006-10 Biotechnology

Science &

Technology

Texas A&M University, College Station, Master of Science 2011-13 Biotechnology

Texas Graduate Student

A. Procedural and Technical Skills

Research: Flow Cytometry, Q-PCR, FISH, ELISA, HPLC, RFLP, FISH, Gas

Chromatography, Gel

Electrophoresis, Western Blotting technique, Mass

Spectrometry, Cloning, Microbial limit tests,

HPTLC (High performance thin layer chromatography), Animal

Handling, Permeability test

Immunostaining, Tissue Culture,

Software: Liquid handling robot (Biomek FX automation), Graph pad prism,

Protocol optimization on Envision

Statistical software- STATLIA, BLAST, Primer express.

B. Research Experience

1. Student technician: Veterinary Pathobiology department, Texas A&M

University: Working on a project on Preeclampsia, a pregnancy

disorder. The work involves quantifying Marinobufagenin (MBG) in blood

and serum samples using ELISA. Conducting permeability study for the

same compound. Receiving shipments of samples and then conducting

immunoassays. Maintaining lab record and discussing the results with

team. Ordering and preparing reagents and buffers for the lab.

2. Assay Development: MD Anderson Cancer Institute, TX (5/2012-11/2012):

Development, optimization, validation and implementation of a 384-well

dehydrogenase assay for IC50 determinations with test compounds in

order to determine selectivity for a drug discovery program for

cancer, involving:

a) High throughput screening- Automation of developed

enzymatic assay using Biomek FX automated workstation.

b) Generation of dose response curves with test compounds

and analyzing and reporting IC50 results to the project

team members.

c) Assessing assay variability for a robust assay.

d) Comparison of IC50 data from this assay to results

obtained by other team members with the target enzyme

assay.

3. Effects of Antioxidants: Texas A & M University, Tx (2/2012-5/2012):

Effect of antioxidants present in various varieties of potato in the

progression of prostate cancer using TRAMP mice model.

4. All India Institute of Medical Sciences, Delhi, India (4/2010-6/2010):

Department of Hematology: Detection of ANXA2 25 Single Nucleotide

Polymorphism in Sickle cell patients.

5. Arbro Analytical Research Divisions, New Delhi, India (4/08-6/08, 4/09-

6/09): Environmental testing with emphasis on

a) Tested for the presence of mycobacterium in various

qualities of packaged drinking water using Real time PCR

and also conducted total viable colony count for presence

of microorganisms.

b) Tested for the presence and estimation of the pesticides

that are present in honey samples using Gas

Chromatography.

c) Conducted High Performance Thin Layer Chromatography

(HPTLC) of herbal capsule known as Hepalina for

quantitative and qualitative estimation.

d) Tested for the presence of pesticides in pomengranate

seeds using HPLC followed by their subsequent detection

using Mass Spectrometer.

e) Using ELISA estimated the level of folic acid in a multi

vitamin capsule to analyze whether it complied with the

claimed concentration of folic acid.

C. Certification

2010 Bachelor of Science and Biotechnology.

2013 Master of Science and Biotechnology.

Certificate of Entrepreneurship, Mays Business School

D. Societies and Professional Membership

2010-12 Member of Biotechnology Society, Texas A &M University,

TX, USA

E. Computer Skills

MS Office, Microsoft Project

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