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Karl

J. Wahlin

****

Eastern Avenue abqsv3@r.postjobfree.com

Baltimore home.410-***-****

MD lab.410-***-****

21224

research

interest

To study molecular and cellular aspects of neurobiology

with a particular focus on synapse formation and retinal connectivity.

work

experience

Wilmer

Eye Institute at the Johns Hopkins Hospital.

Baltimore, MD June

1996-fall 2002

Title:

Research Technician

Conducted

novel in vivo and in vitro research aimed

at studying the molecular mechanisms driving

neurotrophic factor mediated photoreceptor

survival in the mammalian retina. The findings

of this work have direct clinical implications

since they offer insight into the treatment

of retinal dystrophies such as retinitis pigmentosa

and macular degeneration. Additional molecular

techniques were developed to study growth

factor receptor responses at the single cell

level. It is through the collection and isolation

of single retinal cell types that I have been

able to create cDNA pools that are actively

being studied by candidate gene PCR and microarray

analysis.

I have gained expertise in various laboratory

techniques some of which include: Single cell

microcapture, microdissections, Polymerase

Chain Reaction (PCR), primer design, gene

subcloning, recombinant plasmid construction,

plasmid mini- and maxi-prep, DNA sequencing,

synthesis of DNA and RNA probes, in situ hybridization,

Northern hybridization, histological staining,

immunohistochemistry using bright field and

fluorescence microscopy, image analysis using

Image Pro and SPOT RT software, genotyping

of transgenic mouse lines, primary organ cultures,

and intra-vitreal microinjections.

SUNY

Research Foundation.Amherst,

NY Jan.1994-Aug.1995

Title:

Lab Technician / Research Assistant

My

research in the Department of Biochemical

Pharmacology at SUNY Buffalo focused on the

role that retinoic acid plays in the differentiation

of uncommitted stem cells into a pathway that

commits them to neuronal phenotypes. Emphasis

was placed upon expression of Protein Kinase

C isotypes. Other responsibilities were to

manage laboratory inventory, making reagents

and solutions when necessary, and ordering.

Some of the techniques used were PCR, restriction

analysis, ligation, electroporation, cDNA

library synthesis via (RT) reverse transcription,

plasmid contruction and miniprep, production

of radiolabelled probes DNA, RNA, and protein

extraction and isolation, spectrophotometry

(UV/VIS), Lowry assay, microscopy, Northern

and Western Hybridization, autoradiography,

agarose and SDS-PAGE electrophoresis, routine

cell culture, cell counting, preparation of

media and reagents for general lab use, radiation

wipe test surveys, autoclaving.

education

Johns

Hopkins School of Medicine;

Baltimore, MD 21280 September

2002 to present

Department of Neuroscience, PhD candidate

Johns

Hopkins University;

Baltimore, MD 21280 February

2000

Masters

of Science in Biotechnology,

State

University of New York at Buffalo;

Amherst, NY 14227 February

1996

Double

degree: Bachelors of Science in Biology and

Biochemical Pharmacology

Duke

University Marine Laboratory;Beaufort, N.C. 27708 Summer

1992

Course:

Biochemistry of Marine Animals.

other

skills

Advanced

level SCUBA diver.Adult

CPR certification American Red CrossBasic

Radiation Safety - SUNY BuffaloFamiliar

with Macintosh, Windows & UNIX computer systems. Experience

with web development usingFlash, Fireworks, Ultradev, and Photoshop

software packages. Some experience programming in html, asp/javascript,

css scripting languages. Familiar with MS Access, MySQL, and

Filemaker Pro databases.

>publications

Wahlin KJ, Yu, N, Adler R. Expression of Synaptic Molecules in the Developing Chick retina. Manuscript in progress.

Pasterkamp RJ, Dai H, Terman JR, Wahlin KJ, Kim B, Bregman BS, Popovich PG, Kolodkin AL. MICAL Flavoprotein Monooxygenases: Expression in the Developing and Adult Rat Nervous System and Following Spinal Cord Injuries. Mol Cell Neurosci. 2005 Oct 13

Wahlin KJ, Lim L, Grice EA, Campochiaro PA, Zack DJ, Adler R. A method for analysis of gene expression in isolated mouse

photoreceptor and Muller cells. 2004 Mol Vis 10:366-375.

Mori K, Gelbach P, Ando A, Wahlin KJ, Gunther V,

McVey D, Wei L, Campochiaro PA. Intraocular Adenoviral

vector- mediated gene transfer is increased in proliferative

retinopathies. Invest Ophthalmol Vis Sci

2002.

Wahlin KJ, Campochiaro PA, Zack DJ, Adler R. Neurotrophic

factors cause activation of intracellular signaling pathways

in Muller cells and other cells of the inner retina, but not

photoreceptors. Invest Ophthalmol Vis Sci. 2000 Mar;41(3):927-36.

Wahlin KJ, Adler R, Zack DJ, Campochiaro PA. Neurotrophic

Factor Signaling in Normal and Degenerating Rodent Retinas. Exp

Eye Research 2001 Nov;73(5):693-701.

Mathura JR Jr, Jafari N, Chang JT, Hackett SF, Wahlin KJ,

Della NG, Okamoto N, Zack DJ, Campochiaro PA. Bone morphogenetic

proteins-2 and -4: negative growth regulators in adult retinal

pigmented epithelium. Invest Ophthalmol Vis Sci. 2000 Feb;41(2):592-600.

Hackett

SF, Ozaki H, Strauss RW, Wahlin K, Suri C, Maisonpierre

P, Yancopoulos G, Campochiaro PA. Angiopoietin 2 expression

in the retina: upregulation during physiologic and pathologic

neovascularization. J Cell Physiol. 2000 Sep;184(3):275-84.

Review.

presentations

Analysis

of the Site of Action of Neurotrophic Factors in Mouse Retina.

Wahlin KJ. 9th Annual Wilmer Research Meeting, March,

1998

Intraocular Injection of Neurotrophic Factors Activate Signaling

Pathways Within Cells of the inner Retina. Wahlin KJ.

10th Annual Wilmer Research Meeting, 1999

Analysis of Gene Expression in Isolated, Microcaptured Mouse

Photoreceptor and M uller Cells. Wahlin KJ. 13th Annual

Wilmer Research Meeting, 2002

references

Available

upon request

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