Sylvester Siller

Email: abqqqc@r.postjobfree.com

Address: *** ******** ***

City: San Antonio

State: TX

Zip: 78253

Country: USA

Phone: 210-***-****

Skill Level: Experienced

Salary Range: $65,000

Willing to Relocate

Primary Skills/Experience:

See Resume

Educational Background:

See Resume

Job History / Details:

Sylvester Siller

231 Cardinal Way, San Antonio, TX 78253

210-***-****

abqqqc@r.postjobfree.com

Objective Transition from Laboratory to Technical Sales Position

Professional Experience Nucleic Acid Testing Lab Lead/Supervisor Feb 2010-Nov 2012

QUALTEX LABORATORIES, GT/NAT Lab, San Antonio, TX

*Utilized PCR, rtPCR, and TMA molecular diagnostic methods to detect and identify pathogens in blood and blood components

*Procedures included manual and automated extraction methods

*Managed day-to-day operations of lab (Staff scheduling, payroll, workload distribution, resolved atypical testing issue resolution)

*Performed regulation compliance checks on testing procedures

*Personal performance evaluations completed on lab staff

*Review and release of final production testing results prior to customer notification

*Performed equipment maintenance, troubleshooting and repair

*Performed IQ/OQ/PQ of new instrument validation and implementation (This included validation plan development, execution of the validation, and implementation.)

*Trained lab staff on new and established testing processes IAW established SOP and cGMP

Microbiology/QC Lab Lead 2005-2010

SOUTH TEXAS BLOOD&TISSUE CNTR./QUALTEX LABS

Microbiology/QC Lab, San Antonio, TX

*Lab initially functioned as a sterility testing lab for blood components and tissue processing via BioMeriuex BacT Detection and other sterility testing.

*Expanded lab functions to perform blood and blood component QC to determine product viability and regulation compliance

*Further expansion included integration of a Vitek 2 system for identification and AST testing, along with an Observa LIS for interlinking BacT System, report generation, and customer notification.

*In all expansions of this lab, performed primary role in process development, validation, staff training, and SOP implementation.

*Performed all development of offline testing procedures and support equipment validation (ex.; automated gram staining, growth and selection plating, catalase testing, Vitek sample setup, incubator validation, etc.)

Worked directly with clients on aspects of reporting to suit each individual company`s needs.

Education University of Texas Health Science Center San Antonio 2002-2005

Biochemistry Ph.D Candidate

*Completed Preliminaries

*Extensive knowledge in molecular biological reaction development/optimization in a research environment

*Developed complex nucleic acid based reaction experiments to elucidate polymerase structural transitions to during transcription

*Performed site-directed mutagenesis, gene cloning, protein expression/purification (utilizing ion exchange, size exclusion, and affinity columns for purifying desired products), protein characterization (including ELISA, SDS-Page, transcription reactions, crystallization, etc)

Mc Murry University, Abilene, TX 1996-2000

*BS in Biology; Concentration in Biochemistry

Publications -Mechanism of T7 RNA Polymerase Pausing and Termination of the T7 Concatemer Junction: A Local Change in Transcription Bubble Structure Drives a Large Change in Transcription Complex Architecture-, J Mol Biol 2008; Feb 15 376(2) 541-553

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