Assistant University

Peggy Huang

Email: abqnsw@r.postjobfree.com

Address: **** ******* ******* ****

City: Columbia

State: MD

Zip: 21045

Country: USA

Phone: 512-***-****

Skill Level: Experienced

Salary Range: $65,000

Willing to Relocate

Primary Skills/Experience:

See Resume

Educational Background:

See Resume

Job History / Details:

Peggy P.C. Huang

Molecular Biologist and Biochemist with Expertise in Molecular Interaction

US permanent resident

With strong background in molecular biology, biochemistry, and neurobiology, I have extensive research experience in protein-protein/ protein-RNA interactions in neurons, mammalian cells and yeasts. Critical thinking, problem solving, scientific writing and technical innovation are my strengths.

Key Skills

Molecular Biology

Molecular cloning (for bacteria, yeast, mammalian cells, Sindbis virus, and Lentivirus), DNA/RNA/miRNA isolation, RT-PCR, qPCR, microRNA knockdown and overexpression, in vitro transcription and translation, Northern Blot

Biochemistry

Western Blot, protein purification (including GST, HA, His, Flag, and MBP tags), RNA/protein pull down assay, immuno-precipitation, synaptoneurosome isolation, ribosome purification with RNA tags, ribosome (polysome) profiling, ATPase assay using radioisotope nucleotides, ATP binding assay using photo-crosslinkable nucleotides, Luciferase assay, ELISA for blood corticosteroid, phage display

Cell Culture

Primary neuron culture from rat cortex and hippocampus, HEK293T cells, CHO cells, cell culture model for Parkinson`s disease, cell culture transfection and infection

Cell Biology

Immuofluorescence staining (neurons, HEK cells, and yeasts), mammalian cell viability assays, cytotoxicity assays, yeast prion aggregation assay

Microscopy and Imaging

Fluorescence microscopes and confocal (Leica SP5) microscopes. Live imaging, FRET, and imaging analysis using Leica Application Suit software package and ImageJ

Instruments

Real-time PCR machines (Bio-Rad and ABI), Luminometer, Polysome sucrose gradient maker/fractionator, protein purification chromatography (affinity, size-exclusion, ion-exchange, HPLC and FPLC), SPR (BiaCore), fluorescence polarization and spectrophotometer, UV-VIS

Animal Handling

Maintaining transgenic mice colonies for disease models, genotyping, ear tagging, brain dissection from rats and mice

Yeast Genetics

Yeast two-hybrid screen, suppressor screen, yeast tetrad dissection and analysis, yeast prion strains isolation

Computer Software

Microsoft Office (Word, PowerPoint, Excel), EndNote, GraphPad Prism, Adobe Illustrator, Adobe Photoshop, ImageJ, SerialCloner, DNA star, DNA strider, VectorNTI, Sigma Plot, Origin, PyMol, Dreamweaver

Experience

Research Associate

2008-2012

University of Texas at Austin, Austin, Texas

* Studied mTOR-mediated translational control of potassium channel Kv1.1 mRNA in neurons.

* Developed RNA pull down assays to identify miRNA that binds Kv1.1 mRNA.

* Lead undergraduate and graduate students for research and wrote a first-authored paper.

Postdoctoral Fellow

2006-2008

University of Texas at Austin, Austin, Texas

* Studied proteins involved in ribosome biogenesis, performed yeast two-hybrid screen, and developed techniques to affinity purify RNA-tagged ribosomes in yeast.

Postdoctoral Fellow

2004-2006

University of Wisconsin-Madison, Madison, Wisconsin

* Studied functional specificity of J-type chaperones and contributed significantly to a book chapter on Hsp70 chaperones and a review article on J-type chaperone proteins.

Graduate Research Assistant

1998 - 2004

University of Wisconsin-Madison, Madison, Wisconsin

* Characterized novel ribosome-associated chaperones for newly synthesized proteins in yeast using various methods in biochemistry, molecular biology and genetics.

* Tested the roles of molecular chaperones in yeast prion propagation.

Education

PhD, Biomolecular Chemistry

1998 -2004 University of Wisconsin-Madison, Madison, Wisconsin

Thesis: Functional characterization of ribosome-associated chaperones in Saccharomyces cerevisiae

MS, Life Sciences

1996 - 1998 National Tsing Hua University, Hsinchu, Taiwan

Thesis: The study of possible targets of cardiotoxins and phospholipase A2: glycolsamino-glycans on the cell surface and SH3 domain in proteins

BS, Chemistry

1992 - 1996 National Taiwan University, Taipei, Taiwan

Senior project: Competitive binding of drugs to serum albumin by fluorescence anisotropy

Publication/Presentation

Available upon request

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