Manager University

BALAKRISHNAN SHENBAGA MOORTHY

E-mail: abqnfe@r.postjobfree.com

Tel: (65) 97309064

OBJECTIVE

To build a challenging research career in a strong value driven organization where I can utilize

my skills and learn more in the field of biochemistry, structural biology and mass spectrometry.

RESEARCH EXPERIENCE

4 years of research experience in protein-protein and protein-ligand interactions using

biophysical methods

Expert hands-on skills in amide hydrogen/deuterium exchange and mass spectrometry

technique both with MALDI-TOF-MS and LC-ESI-QTOF-MS (Synapt HDMS, Waters)

Extensive experience on in vitro biochemical assays to characterize recombinant proteins

for its activity

Have good experience in recombinant protein purification from bacteria using

chromatographic techniques

Substantial experience in data analysis, documentation and manuscript preparation in the

related field of research

SKILLS AND TECHNIQUES

Protein Biochemistry Biophysical Techniques Software

Protein expression FPLC MassLynx

Protein purification MALDI-MS and LC-ESI-MS PLGS

Gel electrophoresis Fluorescence spec HX-Express

Enzyme activity assays UV, Visible & CD spectroscopy PyMOL

TEACHING EXPERIENCE

Directed two undergraduate students research and supervised their thesis in National

University of Singapore, Singapore.

Taught undergraduate practical, supervised experiments and graded assignments in

National University of Singapore, Singapore.

As a lecturer in Sankaralingam Bhuwaneswari college of pharmacy, India [June 2006 Dec

2006].

PUBLICATIONS

Cooperativity and Allostery in cAMP dependent activation of Protein Kinase : Monitoring

Conformations of intermediates by Amide Hydrogen/Deuterium exchange Mass

Spectrometry Balakrishnan Shenbaga Moorthy, Suguna Badireddy and Ganesh S. Anand

International Journal of Mass Spectrometry, Article in Press, Corrected Proof,

doi:10.1016/j.ijms.2010.09.012

Phosphodiesterases Catalyze Hydrolysis of cAMP bound to Regulatory Subunit of Protein

Kinase A and Mediate Signal Termination Balakrishnan Shenbaga Moorthy, Gao Yunfeng

and Ganesh S. Anand, Molecular & Cellular Proteomics, October 5, 2010, doi:

10.1074/mcp.M110.002295

Basis for the RI mediated activation of Phosphodiesterases and Modeling the interactions :

Using Amide Hydrogen/Deuterium exchange Mass Spectrometry Balakrishnan Shenbaga

Moorthy, Kavitha B, Lin Liqin, Ivana M and Ganesh S. Anand (Manuscript in Preparation)

Dynamics and phosphorylation mediated conformational changes in Phosphodiesterases:

Using Amide Hydrogen/Deuterium exchange Mass Spectrometry Balakrishnan Shenbaga

Moorthy and Ganesh S. Anand (Manuscript in Preparation)

EDUCATIONAL QUALIFICATION

National University of Singapore, PhD (Biological Sciences),

Thesis Title: Molecular interactions and its functional regulations within cAMP dependent

protein kinase A signalling pathway Jan 2007 - Present, [CAP - 3.7]

Anna University, Chennai, India, M. Tech (Biotechnology),

Thesis title: "The in vitro and in vivo study of pVAX BmTGA as DNA vaccine for Human

Lymphatic Filariasis" Jul 2004 - May 2006, [CGPA - 8.696]

M.G.R.Medical University, Chennai, India, B. Pharm (Pharmaceutical Sciences),

Thesis title: "Synthesis of some novel 2-methylthio-3- (substituted Methyl amino) quinazolin -

4(3H)-ones as Anti-Histaminic Agents" Jul 1999 - May 2003, [70%]

CONFERENCES

Presented poster in 6th International Conference in Structural Biology and Functional

Genomics, Dec 2010, National University of Singapore, Singapore

Presented poster titled Monitoring

Conformations of Intermediates by Amide

Hydrogen/Deuterium exchange Mass Spectrometry in Protein Kinase (PKA) Activation at

SSMS DAY 2010, Singapore Turf Club, Singapore

Won the Honorable Mention Award for the poster presentation under Cell Biochemistry

and Molecular Biology session in 14th Biological Science Graduate Congress, Dec 2009,

Chulalongkorn University, Bangkok

Presented poster in Joint 5th Structural Biology and Functional Genomics and 1st Biophysics

International Conference, Dec 2008, National University of Singapore, Singapore

Participated and presented poster at Latest Solutions for Mass Spectrometry organized by

Singapore Society for Mass Spectrometry (SSMS), Nov 2008, Biopolis, Singapore

Presented poster titled Novel regulatory feedback in cAMP Signalling mediated by direct

interactions between Phosphodiesterases and Protein Kinase A Regulatory subunit in 12th

Biological Science Graduate Congress, Dec 2007, University of Malaya, Kualalampur

AWARDS

Graduate Aptitude Test in Engineering (GATE), March 2004, Percentile Score : 98.51

and secured all India rank 122 out of 8669

Received Government Merit Scholarship during my Masters in Technology

NUS Research Scholarship for pursuing my PhD

REFERENCES

Dr. Ganesh Srinivasan ANAND, Dr. Ivana MIHALEK,

Assistant Professor, Principal Investigator,

Department of Biological Sciences, Bioinformatics Institute,

National University of Singapore, 30 Biopolis Street,

14 Science Drive 4, #07-01, Matrix,

Singapore 117543. Singapore 138671.

Email: abqnfe@r.postjobfree.com Email: abqnfe@r.postjobfree.com

(My PhD thesis supervisor) (Research collaborator and advisor)

Dr. Kunchithapadam SWAMINATHAN, Mr. Mark RITCHIE,

Associate Professor, Senior Manager, R&D,

Department of Biological Sciences, Waters Pacific Pte Ltd,

National University of Singapore, #02-08 The Capricorn,

14 Science Drive 4, Singapore Science Park II,

Singapore 117543. Singapore 117528.

Email: abqnfe@r.postjobfree.com

Email: abqnfe@r.postjobfree.com

(Research collaborators)

(X-Ray crystallography module coordinator)

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