Assistant University

Jihong Wang*** Main St., Apt #*, Medford, MA *****

781-***-****(H), 781-***-**** x 7107(O)

URL: http://members.tripod.com/~jh_w/

E-mail: abqjn7@r.postjobfree.com:Rutgers, The State University of New Jersey, New Brunswick

Ph.D. in Chemistry, 1997, M.S. in Chemistry, 1996

GPA: 3.8/4.0

University of Science and Technology of China, Hefei, China

B.S. in Chemistry, 1988EXPERIENCE:

Scientist, Jan. 1999- present

Consensus Pharmaceuticals, Inc., Medford, Massachusetts.

Postdoctoral fellow, Oct. 97 - Jan. 1999

Center for Advanced Biotechnology and Medicine (CABM), University of

Medicine and Dentistry of New Jersey, Piscataway, New Jersey.

Design and Synthesis of antisense peptide-DNA conjugates for Breast

Cancer treatment.

Develop new liquid-phase synthesis methodology to generate soluble peptide

libraries.

In charge of lab safety issues.Graduate Assistant, Aug. 92 - Sept. 97

Department of Chemistry, Rutgers University, New Brunswick, New Jersey.

Played key role in the AIDS drug research team.

Developed a gel shift binding assay for screening small molecules specifically

binding to DNA or RNA.

Designed, synthesized, and screened series of Tat peptide analogs and peptide-oligonuclotide

conjugates targeting TAR RNA of HIV-1.

Discovered a Tat peptide analog that can inhibit HIV-1 replication. (Patent

Pending)

Engaged in setting up cell uptake assay, drug stability assay and drug

delivery system.

Analyzed and interpreted data in terms of pharmacokinetics and pharmacodynamics

model of drug action and its interaction.

Taught undergraduate courses in Experimental Chemistry.Research Assistant, Sept. 91 - Aug. 92.

National Laboratory of Computer Chemistry, Chinese Academy of Sciences,

Beijing, China.

Engaged in Pesticide Database setup and SAR studies.Research Assistant, Jan. 88 - July 91.

University of Science and Technology of China, Hefei, China.

Prepared superconducting thin films by plasma technique and characterized

the structure of corundum type oxide and superconducting oxides by IR and

X-ray diffraction.

Taught undergraduate courses in Physical Chemistry and Crystal Chemistry.HONORS and AWARD:

CABM Fellowship, Rutgers University (1995).

People's scholarship, University of Science and Technology of China (1985

& 1987).SKILLS:

Analytical and Bioanalytical Chemistry:

HPLC purification of peptides, oligonucleotides, and small molecules.

RNA, DNA and Protein Gel Electrophoresis.

TLC, Fluorescence and UV-Vis spectrometry, ELISA.

Protein Sequencing, Amino Acid Analysis.

Developed a quantitative Gel Shift Binding Assay and measure the

dissociation constants of specific binding.

Separated organic reaction mixtures by Extraction, HPLC and Gel Filtration

Chromatography.

Determined molecular weight of peptides and oligonucleotides by MALDI-MS.

Characterized protected amino acid derivatives by NMR.

Characterized the structure of corundum type oxide thin films by

IR.

Characterized the oxide superconductor structure by X-ray Diffraction.

Determined atomic composition of superconducting film by Atomic Absorption

Spectrometry.Synthesis:

Synthesized peptide and oligonucleotide by Solid-Phase Synthesis and Liquid-Phase

Synthesis.

Expertise on conjugation reactions, peptide modifications, and PEG chemistry.

Familiar with Combinatorial Library Syntheses.Molecular Biology:

Carried out Cloning, Restriction Mapping and Analysis, plasmid isolation,

PCR.

Performed RNase H Cleavage Assay and Phosphor Image Analysis.

Working knowledge of cell culture and cell uptake studies.Computer:

Programmed with FORTRAN, BASIC, HTML.

Expertise in Excel, Word, Photoshop, SigmaPlot, Insight II, GCG, and other

applications and database programs.PUBLICATIONS:

J.H. Lai and J. Wang, "Purification and Analysis of Cyclic

Peptide Libraries, and Compositions Thereof". Patent Pending.

J.H. Lai, J.J. Johnson, L. Josephson, E.K. Kolaczkowski, J. Wang,

J.J. Nestor, and S.A. Kates. "Nucleophilic and Conformational Effects of

Amino Acids in Head-to-Tail Cyclic Peptide Libraries", Proceedings of the

6th International Symposium on Solid Phase Synthesis and Combinatorial

Chemical Libraries, (R. Epton, Ed.) Mayflower Scientific Ltd., Kingswinford,

England, in press.

I. Choudhury, J. Wang, S. Stein, A.B. Rabson and M.J. Leibowitz

"Translational Effects of Peptide Antagonists of Tat Protein of HIV-1",

Journal of General Virology, 80(Pt3): 777-82 (1999).J. Wang, S. Stein, M.J. Leibowitz and A.B. Rabson, "Inhibition of

HIV-1 Replication by a Tat RNA-binding Domain Peptide Analog", patent pending.

S-Y. Huang, S. Pooyan, J. Wang, I. Choudhury, M.J. Leibowitz and

S. Stein. "A Polyethylene Glycol Copolymer for Carrying and Releasing Multiple

Copies of Cysteine-Containing Peptides", Bioconjugate Chemistry,

9(5) 612-617 (1998).

I. Choudhury, J. Wang, A.B. Rabson, S. Pooyan, M.J. Leibowitz and

S. Stein, "Inhibition of HIV-1 Replication by a Tat RNA-binding Domain

Peptide Analog", Journal of Acquired Immune Deficiency Syndromes and

Human Retrovirology 17, 104-111 (1998).

C.E. Lau and J. Wang "Alprazolam, Caffeine and Their Interaction:

Relating DRL Performance to Pharmacokinetics". Psychopharmacology

126, 115-124 (1996).J. Wang, S-Y. Huang, I. Choudhury, M.J. Leibowitz and S. Stein.

"Use of a Polyethylene Glycol-Peptide Conjugate in a Competition Gel Shift

Assay for Screening Potential Antagonists of HIV-1 Tat Protein Binding

to TAR RNA", Analytical Biochemistry, 232, 238-242 (1995).

C.H. Tung, J. Wang, M.J. Leibowitz and S. Stein. "Dual-specificity

Interaction of HIV-1 TAR RNA with Tat Peptide-oligonucleotide Conjugates",

Bioconjugate

Chemistry, 6(3) 292-295 (1995).

Y Qian, S Yan, J Wang, P Fu and Z Chen. "An IR Study on Thin Films

of Corundum Type Oxides". Chinese Journal of Chemical Physics, 5(4)

275-280 (1992).

Z. Chen, G Zhou, Z Li, J Wang, D Sun and Y Qian. "Structure Characteristics

of High Tc Superconducting Oxide in (Bi,Pb)-Sr-Ca-Cu-O system". Molecular

Crystals &. liquid Crystals, 184, 401-408 (1990).

W liu, J Wang, Z Yang, Y He, H Zhao, Z Chen and Y Qian. "Preparation

of Superconducting Bi-Sr-Ca-Cu-O Thin Films by RF Sputtering". Chinese

Journal of Low Temperature Physics, 11(6), 464-469 (1989) (in Chinese).IMMIGRATION STATUS:

Permanent resident of the US.

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