TODD J. MENKHAUS, Ph.D.

*** *. **. ****** **.

Rapid City, SD 57701

605-***-****

abqjjx@r.postjobfree.com

EDUCATION

Ph.D. Chemical Engineering, with Honors, December 2002

Iowa State University, Ames, Iowa

Advisor: Dr. Charles E. Glatz

GPA 3.94/A=4.00

B.S. Chemical Engineering, with Honors, December 1998

University of Wyoming, Laramie, Wyoming

GPA 4.00/ A=4.00

CURRENT POSITION

Chemical and Biological Engineering Department: South Dakota School of

Mines and Technology, Rapid City, SD

Assistant Professor August 2005 Present

PROFESSIONAL EXPERIENCE

Process Development and Pilot Plant Operations: Xoma (US) LLC, Berkeley, CA

Scientist II April 2005 August 2005

Scientist I April 2004 March 2005

Summary: Lead activities for selection, process development, scale-up, and clinical

manufacturing of harvest and purification operations for recombinant proteins

produced in microbial and mammalian systems. Processes include: depth filtration,

tangential flow filtration (cell separation and UF/DF), viral filtration, various modes

of chromatography (affinity, ion-exchange, hydrophobic interaction), and

membrane adsorbers.

- Plan, supervise, and perform development activities for recovery and purification

- Plan, supervise, and perform recovery and purification efforts for clinical production

of biologics under cGMP compliance (up to 500 L fermentor scale)

- Construct and validate equipment for cGMP use

- Author SOPs, Batch Production Records, and technical reports

- Transfer processes to manufacturing teams

- Member of project team that developed and implemented a capacity utilization

model to effectively utilize resources and pinpoint bottlenecks from gene cloning

through clinical manufacturing

- Communicate with outside collaborators and vendors to move projects forward and

implement current practices

- Discuss project direction and present experimental results, manufacturing

outcomes, and technical seminars to all levels of management and personnel

throughout the company and with collaborators

- Supervise, mentor, motivate and work with associates

November 2002 April 2004

Research Engineer - Purification Process Development: Regeneron

Pharmaceuticals, Inc., Tarrytown, NY

- Developed and implemented methods for the harvest of crude bioreactor cultures

and subsequent purification of protein for three antibody fusion products

- Developed and implemented analytical tools (e.g. HPLC, GC, ELISA and

colorometric assays), for evaluating protein quality, quantifying product impurities,

and supporting bioreactor and media development groups

- Planed, supervised, and performed small-scale experiments to evaluate and

optimize process alternatives for clarification and purification of protein products;

including centrifugation, depth filtration, chromatography (affinity, ion-exchange,

HIC, and size exclusion), and TFF operations (cell separation and UF/DF).

- Processes successfully scaled to 10,000 L (one product) & 1,500 L (two products)

- Performed harvest and purification of protein for small scale pilot manufacturing

(up to 200 L), including clarification, chromatographic and membrane

separations.

- Planed, performed, and interpreted results for scale-down viral clearance

evaluation of purification processes

- Transferred process recommendations and provided troubleshooting support for

harvest and purification of products to pilot and manufacturing facilities

- Authored batch records and SOPs for use in process development and

manufacturing

- Interfaced with all levels of management to discuss project priorities and direction

- Provided work direction, assigned tasks, and guided associates on harvest and

purification process development issues, including one direct report.

January 1999 - November 2002

Research Associate: Chemical Engineering Dept., Iowa St. Univ., Ames, IA

Ph.D. Thesis "Recovery and Purification of Recombinant Proteins from Plants:

Strategies in Implementation and Optimization"

- Developed and optimized a lab-scale process (with considerations for scale-up) for

the recovery and purification of a human antibody produced in corn. The process

consisted of:

- Processing the plant material, including degermination and particle size

reduction, with pilot scale equipment

- Exploring and implementing conditions for selective extraction of antibody from

corn solids into an aqueous medium

- Evaluating alternative approaches to perform requisite solids clarification

- Assessment of chromatographic resins and binding conditions for use with

expanded bed adsorption (EBA) and packed bed chromatography, including affinity,

ion-exchange, hydrophobic interaction, and immobilized metal affinity (IMAC) modes

- model simulations to predict adsorption performance from small-scale lab

experiments

- Comparison of EBA column designs and operating protocols

- Computer process design simulations using (Bio)SuperPro Designer to perform

initial costing estimates and locate potential bottleneck areas

- Investigated the role plant host plays in the efficiency of polyelectrolyte

precipitation of "spiked" recombinant protein from plant extracts

- Produced recombinant proteins by shake flask fermentation (up to 4 L), utilizing E.

coli as the host organism

- Purified recombinant proteins by preparative scale chromatography

- Supervised and directed nine undergraduate research assistants

SKILLS and TRAINING

- cGMP Manufacturing experience: centrifugation, depth filtration, TFF, UF/DF,

manual and automated chromatography systems, validation of equipment, and

general documentation and compliance issues.

- Development/technical skills include an in-depth understanding and experience

with: Streamline and UpFront EBA units, Biorad and AKTA Explorer FPLC units,

Agilent and Waters HPLC systems, Shimadzu GC, Electrophoresis, UV/Vis

spectrophotometers, ELISA, ultracentrifuges, pilot-scale seed processing equipment

(i.e. seed milling and flaking), and general laboratory equipment

- Proficient in the use of FORTRAN 90, Microsoft Office, MATLAB, SAS,

and Extend and (Bio)SuperPro Designer bioprocess simulation software

- Successfully completed industrial short courses on "cGMP and Quality Issues for

Biopharmaceuticals", and "Global Leadership Training", and Time Management for

Managers

- Successfully completed the Fundamentals of Engineering exam

TEACHING and MENTORING EXPERIENCE

SDSM&T Teaching Activities

- CHE 217: Chemical Engineering I (Material and Energy Balances)

O Fall 2005: 27 Students

- CHE/EnvE 318: Chemical Engineering IV (Mass Transfer and Unit Operations)

O Spring 2006: 15 Students

- CHE 484/484 and 484L/584L: Biochemical Engineering Lecture and Lab

O Spring 2006: 14 Students

Taught two week bioseparations section

Developed and taught two lab units on recovery and

separations

- Graduate Students

O Master s

Navody Ravikanti: Fall 2005

Development of Bioseparations Laboratories

- Graduate Student Committees

O Spring 2006: One (1)

January 2002 December 2002

Preparing Future Faculty Fellow, Iowa State Univ., Ames, IA

- One of 30 graduate students selected campus-wide to participate in a faculty

seminar and mentoring series designed to introduce graduate students to life as a

professor

- Attended weekly seminars discussing topics such as balancing faculty expectations,

student learning styles, lecturing, technology in the classroom and faculty service

- Attended weekly mentoring sessions with Dr. Charles Glatz in chemical engineering

to discuss topics such as research funding/grant writing, teaching in chemical

engineering, and developing class assignments and tests

August 2002 - December 2002

Co-Instructor: Bioseparations, Iowa State Univ. Chem. Eng. Dept., Ames, IA

- Lectured three times weekly for 50 minutes on bioseparations topics

- Developed, assigned, and graded homework, quizzes and tests

- Class of 20 students

June 2001 - July 2001

Co-Instructor: ISU Chemical Engineering Department Foreign Study Program,

University College London (UCL), London, England, UK.

- Acted as instructor and chaperone of 30 Iowa State Univ. undergraduate students

for unit operations lectures and laboratories at UCL, and industrial tours of the

United Kingdom

- Conducted daily tutorial sessions and graded reports, presentations, and projects

January 2001 - July 2001

High School Student Recruiter: Iowa State Univ. Chem. Eng. Dept., Ames, IA

- Member of graduate student team that prepared presentations to explain chemical

engineering concepts to high school students

- Traveled to area high schools to give presentations and discuss chemical

engineering concepts

- Prepared and gave introductory lectures, and performed lab experiments for the

Engineering Honors Workshop designed to introduce high school students to basic

engineering principles.

January 2000 - May 2000

Teaching Assistant: Chemical Engineering Lab 2, Iowa State University, Ames, IA

- Instructed and managed nine laboratory experiments for undergraduate unit

operations class

- Tutored and graded reports for 17 undergraduate chemical engineering students

September 1998 - December 1998

Teaching Assistant: Chemistry I Laboratory, Univ. of Wyoming, Laramie, WY

- Directed a weekly laboratory session of 21 undergraduate students

- Responsible for one 50 minute weekly lecture, daily tutorial sessions, helping

students with experiments, and grading laboratory reports, homework

assignments, and exams.

PUBLICATIONS

1. Menkhaus, TJ, and Glatz, CE. Antibody Capture from Corn Endosperm Extracts

by Packed Bed and Expanded Bed Adsorption. Biotechnology Progress, 21(2), 473

485 (2005).

2. Menkhaus, TJ, Bai, Y, Zhang, CM, Nikolov ZL, and Glatz, CE. A review:

Considerations in the recovery of recombinant proteins from transgenic plants.

Biotechnolgy Progress, 20(4), 1001 1014 (2004). ** The 11th most-accessed

article in Biotechnology Progress during 2004.

3. Menkhaus, TJ, Pate, C, Kretch, A, and Glatz, CE. Recominant protein purification

from pea. Biotechnolgy and Bioengineering, 86(1), 108 114 (2004).

4. Menkhaus, TJ, and Glatz, CE. Compatibility of Column Inlet and Adsorbent

Designs for Processing of Crude Corn Extract by Expanded Bed Adsorption.

Biotechnology and Bioengineering, 87(3), 324-336 (2004).

5. Menkhaus TJ, Eriksson SU, Whitson PB, and Glatz CE. Host selection as a

downstream strategy: Polyelectrolyte precipitation of beta-glucuronidase from plant

extracts. Biotechnology and Bioengineering, 77(2), 148-154 (2002).

6. Menkhaus TJ, Hrma PR, and Li H. Kinetics of nepheline crystallization from high-

level waste glass. Ceramic Transactions 107: 461-469 (2000).

PRESENTATIONS

1. Menkhaus, TJ. Engineering unit operations for recovery of pharmaceutical

proteins from transgenic agriculture. South Dakota School of Mines and Technology

Fall 2005 Seminar Series, Rapid City, SD, October, 2005 (invited).

2. Menkhaus TJ and Glatz, CE. Considerations for the application of

expanded bed adsorption in the recovery of a recombinant protein from maize.

Presented at the 224th ACS National Meeting, Boston, MA, August 2002.

3. Menkhaus, TJ. Recovery of recombinant proteins from transgenic plants:

Strategies in optimization. Presented at Monsanto, St. Lous, MO, July 2002. (Invited)

4. Menkhaus TJ, Bai Y, and Glatz, CE. Processing strategies for recovery and

purification of recombinant proteins from plant hosts. Presented at the AIChE annual

meeting, Reno, NV, Novermber 2001.

5. Menkhaus TJ, Zhang C, Bai Y, and Glatz, CE (presenter). Recombinant proteins

from plants - what will this mean for recovery? Presented at IBC International

Conference on Recovery and Purification of Biopharmaceuticals, San Diego, CA,

November 2001. (Invited).

6. Bai Y, Menkhaus TJ, and Glatz, CE (presenter). Matching unit operations to plant

hosts for recovery of recombinat proteins. Poster presented at Recovery of Biological

Products X, Cancun, Mexico, June 2001. (Invited)

7. Menkhaus TJ, Eriksson SU, Whitson PB, and Glatz, CE. Polyelectrolyte

precipitation of wild-type and charge-modified beta-glucuronidase from corn, canola,

and soy extracts. Presented at the AIChE Annual Meeting, Los Angeles, CA,

November 2000.

8. Menkhaus TJ, Eriksson SU, Whitson PB, and Glatz, CE. Polyelectrolyte

precipitation of wild-type and charge-modified beta-glucuronidase from corn, canola,

and soy extracts. Presented at 30th Annual Biochemical Engineering Symposium,

Boulder, CO, September 2000. Selected 2nd place best presentation award.

HONORS AND ACTIVITIES

- Awarded the Excellence in Graduate Research honor, Awarded to top 5% of

graduate research at Iowa State University, December 2002

- Premium for Academic Excellence Fellowship, 6 semesters of graduate study

- Boeing Dissertation Fellowship, 1 semester of graduate study

- Graduate Student Senator, 3 semesters

- Financial Chair, Chemical Eng. Graduate Student Organization, 4 semesters

- Visitation Coordinator and Graduate Student Recruiter, 4 semesters

- Department Safety Committee graduate student representative, 2 semesters

- Member, American Institute of Chemical Engineers

- Member, American Chemical Society

- Awarded the Univ. of Wyo. Chemical Engineering Department Top Graduate, 1998

- Awarded the Univ. of Wyo. Outstanding Freshman Engineering Student, 1995

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