Assistant Technician

JEANNETTE N. STANKOWSKI

Home: Work:

**** ******* ** *****, ***. 624 465 21st Avenue South

Nashville, TN 37221 MRB III, Room 8150

615-***-**** Nashville, TN 37232

abqhju@r.postjobfree.com 615-***-****

EDUCATION

Vanderbilt University, Nashville, Tennessee

Doctoral Degree Candidate, Neuroscience Graduate Program

Thesis Title: Role of HSP70 Interacting Protein CHIP in Determining Cell Fate Following Acute Ischemic

Injury.

Relevant Coursework: Cellular and Molecular NeuroscienceAdvanced Molecular Neurobiology

Drew University, Madison, New Jersey

Bachelor of Arts, Dual major in Biology and French, May 2004, GPA: 3.6/4.0

Relevant Coursework: Cell and Molecular NeurobiologyAnatomy and Physiology of Neural SystemsBehavior and Experience: An Intro to PsychologyChemistry (Inorganic and Organic)

Activities:Beta Beta Beta (Biology National Honor Society)Pi Delta Phi (French Honor Society).

HONORS AND AWARDSSociety for Neuroscience 2009 National Chapter Graduate Student Travel Award, August 2009Vanderbilt Kennedy Center Travel Award for VKC Affiliates, March 2009Neurotoxicology Specialty Section of Society of Toxicology Conference Award, July 2008Vanderbilt Kennedy Center Travel Award for VKC Affiliates, January 2008Vanderbilt University Neuroscience Departmental retreat best poster by a graduate student in cellular and molecular neuroscience Award, June 2007Neuroscience Graduate Student Travel Grant, October 2006Vanderbilt Predoctoral Training Fellowship, 2006-2008Novartis Science Award for Excellence in Neuroscience, April 2004 – Drew UniversityDean’s List, (7 out of 8 semesters) – Drew UniversityEverett Balch Endowed Scholarship, 2003-2004 – Drew UniversityDrew Recognition Award, 2001-2004 – Drew UniversityRichard and Pat Fowler Scholarship, 2000-2004 – Drew University

TECHNIQUESCell Culture (neural cell lines and primary neuronal cultures)Detection of glutathione levels using the HPLC methodologyDNA/RNA extraction from cells and tissuesExtraction and Generation of DNA from transformed E.coliTransfections (Nucleofection, Lipid-based transfections)Generation of Stable Cell linesSmall interfering RNA methodologyProtein carbonyl assays using the OxyBlot methodologyBiotin-avid capture and immunoblotting methodology Generation and maintenance of hippocampal brain slicesInduction of acute injury (oxidative and denaturing) in neural cell lines and primary neuronsIn vitro toxicology assays (LDH assay, MTT assay)

PUBLICATIONS

Stankowski, J.N., Zeiger, S.L. and McLaughlin, B. Mitochondrial status and oxidative damage. Cell Culture Techniques. Editor: Aschner, M. [In preparation]

Stankowski, J.N., Codreanu, S.G., Liebler, D.C. and McLaughlin, B. Effects of the E3 ubiquitin ligase CHIP on protein oxidation and antioxidant status. [In preparation]

Stankowski, J.N., and McLaughlin, B. Role of Hsp70 interacting protein CHIP in determining cell fate following ischemic injury. [In preparation; to be submitted November 2009].

Stanwood, G.D., Leitch, D.B., Savchenko, V., Wu, J., Fitsanakis, V.A., Anderson, D.J., Stankowski, J.N., Aschner, M., and McLaughlin, B. (2009) Manganese Exposure is Cytotoxic and Alters Dopaminergic and GABAergic Neurons within the Basal Ganglia. Journal of Neurochemistry.

Stankowski, J.N., Leitch, D.B., Aschner, M., McLaughlin, B. and Stanwood, G.D. (2008) Selective Vulnerability of dopaminergic systems to Manganese: Relevance to occupational exposure. Neurotoxicology and Teratology, 30: 259.

ABSTRACTSPOSTER: Role of HSP70 Interacting Protein CHIP in Determining Cell Fate Following Ischemic Injury. Stankowski, J.N., Cohen, E., Codreanu, S.G., Liebler, D.C. and McLaughlin, B.M. Annual Meeting of the Society for Neuroscience (2009).TALK: Selective Vulnerability of Dopaminergic Systems to Manganese: Relevance to Occupational Exposure. Stankowski, J.N., Leitch, D., Aschner, M., McLaughlin, B.M. and Stanwood, G.D. Thirty-second Annual Meeting of the Neurobehavioral Teratology Society (2008).POSTER: Role of HSP70 Interacting Protein CHIP in Determining Cell Fate Following Ischemic Injury. Stankowski, J.N. and McLaughlin, B.M. Annual Meeting of the Society for Neuroscience (2006).POSTER: Interaction between HSP70 and CHIP Leads to Protection Against Cell Death. Stankowski, J.N. and McLaughlin, B.M. Molecular Chaperones and Heat Shock Response in Cold Spring Harbor (2006).

CONFERENCESSociety for Neuroscience Conference, October 15-21, 2009Vanderbilt Kennedy Center Science Day, January 29, 2009Thirty Second Annual Meeting of the Neurobehavioral Teratology Society, June 28-July 2, 2008Vanderbilt Kennedy Center Science Day, November 27, 2007Society for Neuroscience Conference, October 13-18, 2006Cold Spring Harbor Laboratory, Conference for Molecular Chaperones and the Heat Shock Response, May 3-7, 2006

PROFESSIONAL MEMBERSHIPSVanderbilt Kennedy Center Graduate Student Affiliate, November 2007-currentMember of Middle Tennessee Chapter Society for Neuroscience, June 2006-currentMember of Society for Neuroscience, May 2006-current

ADDITIONAL EXPERIENCE

United Nations Volunteers, St. Louis, MO 02/05-07/05

Translator

Translated documents in the field of Economics from English into German; Translated documents in the field of Global Issues and Economics from English into French.

Drew University, Madison, NJ, 01/04-03/04

Laboratory Technician

Prepared materials for all biology laboratories; Animal care.

Drew University, Madison, NJ 09/03-12/03

Teacher Assistant

Worked in the laboratory to prepare required materials for Prof. Knowles’ neurobiology class and

assisted during laboratory periods.

Human Genetic Research Institute, Oberhausen, Germany, Summer 2003

Internship, Genetic Analyst

Examined metaphase chromosomes to determine chromosomal abnormalities in embryos; Examined

FISH probes for the presence of Trisomy 13, 18, and 21.

Drew University, Madison, NJ, 09/02-05/03

Peer Tutoring Program, Tutor (French, Physics), Typist

Worked on one-to-one basis with tutees and made material comprehensible to tutees.

LANGUAGES/INTERESTS

Native German speaker (German Abitur); fluent in English, French and Polish. Enjoy traveling, languages, running, dancing and skiing.

REFERENCES

Dr. BethAnn McLaughlin, Assistant Professor of Neurology and Pharmacology.

EMAIL: abqhju@r.postjobfree.com; PHONE: 615-***-****.

Dr. Pat Levitt, Provost Professor of Neuroscience, Psychiatry and Pharmacy.

EMAIL: abqhju@r.postjobfree.com; PHONE: 323-***-****.

Dr. Mark Wallace, Associate Professor of Hearing and Speech; Director, Vanderbilt Brain Institute.

EMAIL: abqhju@r.postjobfree.com; PHONE: 615-***-****.

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