Plant Assistant

CURRICULUM VITAE

Kedong Da

WORK ADDRESS

NESPAL-UGA

Molecular Cotton Breeding Lab

P. O. Box 748

Tifton, GA 31794-0748

Tel: 229-***-****

Fax: 229-***-****; E-mail: ****@***.***

TITLE Research Professional

EDUCATION

Shandong Agricultural University, China Ph.D. (Horticulture) 1999

Shandong Agricultural University, China M.S. (Horticulture) 1994

Gansu Agricultural University, China B.S. (Horticulture) 1990

RESEARCH INTEREST

My research interest is on somatic embryo induction and genetic transformation of crops. Although currently work on cotton, I have additional experience in transformation of apple, orchid, petunia, begonia, cabbage and chive. I am the first reporter of somatic embryogenesis in apple and begonia. Histological study proved single cell origin of apple somatic embryogenesis. In the area of transformation, both Agrobacterium-mediated (mostly apple, orchid, petunia, begonia, cabbage and chive) and particle bombardment (mostly orchid and cotton) methods were used for transformation. Efforts on improve transformation efficiencies are continuing by optimization of tissue culture regeneration condition and use of GFP visual selection. Valuable information was gaining on single cell transformation and anti-biotic selection free transformation.

PROFESSIONAL EMPLOYMENT

NESPAL-UGA Cotton Molecular breeding Lab 2004-present

Tifton, Georgia

Post-doc Research Associate

Mentor: Dr. Peng W. Chee

Konkuk University, Department of Horticulture 2003-2004

Seoul, Korea Republic

Senior researcher

Mentor: Dr. Doohwan Kim

Swedish University of Agriculture, Dept. of Horticulture 2003

Alnarp, Sweden

Visiting scientist

Mentor: Dr. Li-Hua Zhu

Shenyang Agricultural University, College of Horticulture 2001-2003

Shenyang, China

Post-doc Research Associate

Mentor: Dr. Deguo Lu

Shandong Agricultural University, College of Horticulture 1999-2003

Taian Shandong, China

Associate Professor (faculty)

Mentor: Dr. Futian Peng

Shandong Agricultural University, College of Horticulture 1994-1999

Taian Shandong, China

Assistant (1994-1997), Lecturer (1997-1999) (faculty)

Mentor: Dr. Futian Peng

Department of Agriculture, Gansu Province 1990-1991

Lanzhou Gansu, China

Secretary

Mentor: Mr. Xiuzhi Yuan

RESEARCH EXPERIENCE

Plant-insect interaction 2005-present Evaluation of insect damage to corn yield.Physiological response of corn plants to insect damage.

Cotton Tissue Culture and Biotechnology 2004-presentGreen fluorescent protein (GFP) gene as a visual selection marker for genetic transforamtion of elite Georgia cotton lines.Development of tissue culture regeneration and genetic transformation protocols for elite Georgia cotton lines.

Orchid Tissue Culture and Biotechnology 2003-2004Genetic transformation of Phalaenopsis orchid with flower color related genes. Tissue culture, micropropagation and pot plant production of Phalaenopsis orchid.

Ornamental Plant Tissue Culture and Biotechnology 2000-2003Tissue culture regeneration, micro-propagation and pot plant production of begonia (Begonia X Elatior)Tissue culture regeneration, micropropagation and pot plant production of trailing petunia (Petunia hybrida)Tissue culture micropropagation of lisianthus (Eustoma grandiflorum)Tissue culture, micropropagation and pot plant production of Snningia speciosaTissue culture micropropagation of Amaryllis vittataTissue culture, micropropagation and pot plant production of mini-rose (Rose hybrida)Agrobacterium mediated transformation of begonia (Begonia X Elatior) with CpTI gene.Agrobacterium mediated transformation of petunia (Petunia hybrida) with CHS gene.Agrobacterium mediated transformation of Chinese chive (Allium tubersume Rottle) with CpTI gene.

Fruit Tree Biotechnology 1996-1999Construction of super expression binary vector harboring (CpTI) gene.Apple tissue culture and plant regenerationAgrobacterium mediated transformation of appleRAPD analysis of somaclonal variation in appleBAC library construction of dwarf appleAnalysis of DNA fingerprint between sport apple and its parent by RAPD and AFLP.Isolation of genes with grape nematode resistance by PCR

Fruit Tree Culture and Breeding 1991-1996Tissue culture of fruit trees, including apple, cherry, grape Research on germplasm resources of gingkoMutation breeding of apple and chess-nut

TEACHING EXPERINECE

Special English for M.S student 2002

Plant propagation for B.S student 2002

Ornamental plant tissue culture for B. S student 2001

Plant transformation for M. S student 2001

Plant breeding for B.S student 1999

Fruit tree cultivation for B.S student 1998, 2000

Plant mutation breeding for B. S student 1996, 1997

PUBLICATIONS

Refereed Publications

Invited Book Chapter:

Da K. Plant tissue culture and mutation breeding. In Cheng Xuesen (eds) (2002), Horticultural Plant Breeding, Shandong sci. & tech. publisher. China.

Proceeding Chapters:

Da K. Shu HR. Progress in apple somatic embryogenesis. Zhu Dewei (eds) (2001)

International Proceedings in Biotechnology Application in Horticultural Plants. China Forestry Publisher. Beijing, China.

Research Articles:

Xinzhi Ni, M.D, Krakowsky, Kedong Da. 2005. Evaluation of Corn Hybrids for Resistance to Insects. http://www.griffin.uga.edu/swvt/2005/cn05/RR701-insect.pdf

Yunxiang Zang, Weiwei Zheng, Kedong Da. 2004 Effect of different plant growth regulators on apple roo ting in vitro. J. Shihezi Univ. 22 3 201 203

Yunxiang Zang, Weiwei Zheng, Kedong Da. 2004 Progress on biochemical change during plant somatic embryogenesis. J Shandong Agri. Univ. 35(1):131-136.

Yunxiang Zang, Weiwei Zheng, Kedong Da. 2004 Progress on apple leaf culture and regeneration. Biotechnology. 2 :15 18.

Yunxiang Zang, Song Zhang, Kedong Da. 2004 Studies on leaf regeneration, micropropagation and pot plant production in trailing petunia (Petunia Hybrida). Acta Agriculturea Nuclestae Sinica. 18 1 :18 21.

Da K., Zhang S.et al 2003 Leaf culture, propagation and pot plant production in Lisianthus (Eustoma grandiflorum). J. Shandong Agric. Univ. 34 4 :494 498

Song Zhang, Kedong Da, Chengxing Cao 2003 Agrobacterium tumefaciens-mediated transformation of Chinese chive (Allium tuberosum Rottle) Acta Horticulturae Sinica. 2003, 30(1): 39-42

Song Zhang, Kedong Da, Chengxing Cao. 2003. Effects of antibiotics on plant regeneration on Chinese chive (Allium tuberosume Rottle). Acta Agriculturea Nucleatae Sinica. 2003, 17(2): 101-104

Song Zhang, Kedong Da, Chengxing Cao l. 2002. Efficient plant regeneration via root tip culture on Chinese chive (Allium tuberosume Rottle). Acta Horticulturae Sinica. 29(2): 141-144

Song Zhang, Kedong Da, Chengxing Cao. 2002. Rapid micropropagation system via in vitro culture on Amaryllis vittata and its embryogenesis. Acta Horticulturae Sinica. 29(3): 285-287

Kedong Da, Song Zhang.. 2002. Construction of CpTI gene super expression binary vector and transfer into apple. Proceeding of International Apple Symposium. J. Shandong Agric. Univ. (Suppl): 175-178

Kedong Da, Song Zhang.. 2002. Study on adventitious shoot regeneretion and micro-propagation from in vitro leaves of Begonia Elatior. J. Shandong Agric. Univ. 33(1): 93-95

Song Zhang, Kedong Da, Yutang Wei, Fujiang Wen. 2002. Shoot regeneration of various genotypes in vitro and optimization of hormone concentration and combination in Chinese cabbage. J. Shandong Agric. Univ. 33(1): 7-13

Kedong Da, Song Zhang. 2001. Soamtic embryogenesis from leaves of Begonia Elatior. Acta Horticulturae Sinica. 28(2): 180-181

Kedong Da, Decai Cui, Song Zhang. 2001. Transformation of apple using supper expression cowpea trypsin inhibitor (CpTI) gene. Acta Horticulturae Sinica. 28 (1): 57-58

Kedong Da, Song Zhang, Yazhi Li. 2001. Wounding induced efficient direct somatic embryogenesis in apple leaves. Acta Agriculturea Nucleatae Sinica. 15(5): 290-293

Kedong Da, Song Zhang, Huairui shu. 2001. Progress in apple embroidgenesis. International Symposium on Biotechnology Application in Horticultural Crops. Science and Technology Publisher, Beijing, China. P43-47

Kedong Da, Song Zhang, Yazhi Li. Zengjun Qi. 1996. Direct somatic embryogenesis from in vitro leaves of apple. Acta Horticulturae Sinica. 23(3): 241-245

Kedong Da, Song Zhang, Yazhi Li, Huairui Shu. 1996. Somatic embryogenesis from petiole in apple. Acta Agriculturea Nucleatae Sinica. 10(2): 75-78

Oral presentation

Kedong Da, Xinzhi Ni, David Buntin, and Steve Brown.

Morphological and physiological response of corn seedlings to brown and southern green stink bug feeding. 70th annual meeting for georgia entomological society. March 29-31, 2006. Jekyll Island, GA.

Kedong Da, Jennifer McCurdy, Lloyd May, Peggy Ozias-Akins,

and Peng W. Chee. Development of plant regeneration and transformation protocols for an elite Georgia cotton line. 2006 Beltwide Cotton Conferences. Jan. 3-6, 2006. San Antonio, TX.

Kedong Da, Song Zhang and Huairui Shu. Somatic embroids in Apple. 2nd Asia Pacific Conference in Plant Cell Tissue and Organ Culture. Beijing 1996

Posters:

Kedong Da, Peggy Ozias-Akins, and Peng Chee. Green Fluorescent Protein as a Visual Selection Marker for Cotton Transformation. The ASA-CSSA-SSSA International Annual Meetings. November 6-10, 2005. Salt Lake City, UT

Kedong Da, Peggy Ozias-Akins, Jennifer McCurdy and Peng W. Chee. Genetic transformation of an elite Georgia cotton line. 2005 In Vitro Biology Meeting. June 5th-7th 2005, Baltimore, Maryland.

Kedong Da, Sug-youn Mao, Kyung-Jin Joung, Doo Hwan Kim. High quality protocorm like body (PLB) induction in Phalaenopsis orchid tissue culture. 2004 Meeting of Korea Society of Plant Breeding. Seoul, Korea Republic.

Kedong Da, Sug-youn Mao, Kyung-Jin Joung, Doo Hwan Kim. Factors affecting Agrobacterium mediated high frequency transformation of Phalaenopsis orchid. 2004 Meeting of Korea Society of Plant Breeding. Seoul, Korea Republic.

Kedong Da, Sug-youn Mao, Kyung-Jin Joung, Doo Hwan Kim. Agrobacterium-mediated transformation of Phalaenopsis orchid with chalcone synthase gene (CHS). 2004 Meeting of Korea Society of Biotechnology. Seoul, Korea Republic.

Research Manuscripts in Preparation:

Kedong Da, Song Zhang.. Somatic Embryogenesis in Apple. Plant Cell Reports.

Kedong Da, Song Zhang.. Histological Study of Apple Somatic Embryogenesis Revealed Single Cell Origin. Plant Cell Reports.

Kedong Da, Peggy Ozias-Akins, Jennifer McCurdy and Peng W. Chee. Genetic Transformation of an Elite Georgia Cotton Line. In Vitro Plant.

Kedong Da, Peggy Ozias-Akins, Jennifer McCurdy and Peng W. Chee. Green Fluorescent Protein as a Visual Selection Marker for Cotton Transformation. Plant Cell Reports.

Reference:

Peng W. Chee

Assistant professor

Department of crop and soil science

University of Georgia, Tifton campus

Tifton, Georgia 31794

USA

Tel: 229-***-****

E-mail: ******@******.***.***

Peggy Ozias-Akins

Professor

Department of Horticulture

University of Georgia, Tifton campus

Tifton, Georgia 31794

USA

Tel: 229-***-****

Email: *****@******.***.***

Doohwan Kim

Professor

College of Horticulture

Konkuk University,

1Hwayang-dong, Gwangjin-gu, Seoul 143-701

Korea

Tel: 82-2-450-3740

E-mail: *****@******.**.**

Huairui Shu

Professor

College of Horticulture

Shandong Agricultural University

Taian Shandong 271018

P.R China

Tel: 86-538-*******

E-mail: *****@****.***.**

Contact this candidate