Assistant Technician
Resume:
Rhonda Howard
Email: *********@********.***
Address: **** ********* *****
City: Columbia
State: MO
Zip: 65201
Country: USA
Phone: 512-***-****
Skill Level: Experienced
Salary Range: $60,000
Willing to Relocate
Primary Skills/Experience:
See Resume
Educational Background:
See Resume
Job History / Details:
RHONDA HOWARD, MB(ASCP)CM
512-***-**** * *******@*******.***
EDUCATION
ASCP Certification - Molecular Biology (MB) 12/12
Bachelor of Science in Biochemistry 08/09
Texas State University - San Marcos, Texas
Minor in Biology
Overall GPA: Cum Laude 3.64
Associates of Science in Chemistry 11/07
Austin Community College - Austin, Texas
Overall GPA: Cum Laude 3.7
EXPERIENCE
Clinical Pathology Laboratories (CPL), Austin, TX
Test Development and Method Validation Scientist 11/01/10 - present
* Propose, coordinate, conduct, and supervise the development of new assays for implementation.
* Assess, critique, and edit clinical validation study protocols and procedures using scientific peer review publications, CLIA, CMS, and CAP as a guideline.
* Verify and establish the accuracy, precision, analytical specificity, sensitivity, analytical interferences, clinical reportable range (AMR, CRR), reference interval, calibration criteria for new assays of the Analytical Chemistry Esoteric Department in accordance with the FDA`s requirement; to ensure patient testing acceptability.
* Conduct extensive analysis of clinical and statistical data for verification into validation reports and ensuring auditable quality after completion of testing for the Medical Laboratory Director`s approval.
* Assisted in the development of clinical report templates included SOPs, linearity protocols, and instrument to instrument procedures
* Write technical documents including SOPs, Competency Assessments, Technical Media Lab questionnaires and supervisor key, Client Communications, and Clinical Study Validation Reports.
* Principal analyte Scientist for stability studies including DAUs & TDMs to verify and/or extend Manufacturing Inserts.
* Maintain a strict timeline for new assay being implemented in-house.
* Serve as a scientific consultant and a liaison between the all relevant clinical departments.
* Method Validation Clinical Specialist for the Microbiology, Special Chemistry, Hematology, Special Chemistry, Auto Chemistry, and Stat Lab departments. Successful Validation of high complexity tests include G6PDH, Lactate, Uric Acid, H Pylori BT [CLIA Exempt], DNA Probe Vaginal Pathogens DNA Panel, Myoglobin, Anti-Mitochondrial Abs, APS Panel (Phosphatidylserine Ab IgG, IgA, and IgM [FDA approved], Phosphatidylinositol and Phosphatidylglycerol Ab IgG, IgM, IgA [RUO]), Coombs Test (IgG, C3), Indirect Coombs, ABO/Rh.
* Supervise, train, and oversee Technologists and Department Supervisors on activities of newly implemented test.
* Manage and train staff on techniques and instrument usage required to perform newly -in-house- assay assignments.
* Serve as a Technical Validation Consultant for the development of current/future assays and technical reports.
Pharmacetical Product Development (PPD), Austin, TX
Pharmacokinetic Research Lab Technologist 01/10 - 10/10
* Followed and maintained a working understanding of Good Clinical Practices that are in accordance to Standard Operating Procedures
* Sustained work flow by conducting the Blood Harvesting procedure for the processing and packaging of plasma/serum to the appropriate analytical labs.
* Assisted in the training of new hire to perform the Blood Harvesting protocol and Blood Running collection.
* Effectively managed subjects to respective urine stations while monitoring the collection/processing of urine aliquots.
Agilent Technologies, Cedar Creek. TX
Manufacturing Associate (contract position) 09/09 - 10/09
* Utilized standard molecular biology techniques such as electrophoresis, PCR, DNA and RNA isolation, and Absorbance/ Nano-Drop Spectrophotometry.
* Prepared specialized plasmid vectors such as pERV3 Receptor Vector DNA, and pAP-1-hrGFP Cis reporter plasmid for bacterial transformation procedures.
* Tested PCR Script PCR purification kits and PCR Script Cam sk /Amp Cloning kits to pass QC specs and ensure customer satisfaction
* Confirmed antibiotic resistance from specific bacteria transformations on Ampicillin, Chloramphenicol, Kanamycin, and Tetracycline plates.
* Verified quality of bulk and packaged DNA by performing nuclease reaction test from contamination by endonuclease that can degrade plasmids during shipping.
* Confirmed the size of plasmids from an electrophoresis analysis after a restriction enzyme digestion on post DNA aliquots for packaging
* Answered technical questions about products while adhering to industry, government standards, SOP, and ISO environment.
* Operated sterilization machinery such a Autoclave and enforced safe handling of biohazardous and radioactive materials.
* Followed Work Instructions and accurately documented results.
Texas State University
Professor`s Assistant for Inorganic Chemistry 01/09 - 05/09
* Trained, managed, and tutored freshman and sophomore-level college students in distillation, acid base titration, recrystallization, and purification of specific inorganic compounds.
* Achieved over a 90 % student passing rate in lecture and laboratory exams
* Edited Laboratory reports before submittal for final grading to respective professors.
* Motivated students and enabled conceptual understanding for discerning chemistry techniques
Austin Community College (ACC), Austin, TX
Biology Lab Technician II 06/06 - 04/07
* Prepared, assembled, and instructed experiments for Cellular Molecular Biology, Structure and Function, and Microbiology.
* Assisted and maintained organization of daily laboratory activities to ensure safety.
* Trained and managed work study students.
Austin Community College (ACC), Austin, Texas
Chemistry Lab Technician I 04/05 - 06/06
Austin Community College, Austin, Texas
* Devised and improved laboratories for General Chemistry I & II, and Organic Chemistry I & II.
* Clarified, intervened, and informed proper laboratory procedure and protocol for guarantee protection.
UNDERGRADUATE RESEARCH/PUBLICATIONS
Texas State University - San Marcos, Texas
Research Scientist - Chemistry and Biochemistry Department 05/08 - 05/09
Publication: The FASEB Journal
Optimization of SPR-based assay for monitoring SV40 large T-antigen helicase activity
Rhonda Howard, Jason R Plyler, Steven Williams, Naoko Araki and Wendi M. David
Publication: The Journal of Bio Tech Research
Optimization of surface plasmon resonance based assay for investigating T-antigen helicase activity
Jason R. Plyler Fatemeh Sanjar, Rhonda Howard, Naoko Araki, Wendi M. David
National Fish Hatchery - San Marcos, Texas
Research Scientist - Biology Department at Texas State University Summer 2007
Publication: The Southwestern Naturalist
Consumption of Eggs of the endangered fountain darter (Etheostoma fonticola) by Native and Non-native snails
T. Phillips, *M. L. Alexander, and R. Howard
Texas State University - San Marcos, Texas
Research Assistant - Biology Department at Texas State University Spring 2007
Conducted and developed experiments that identified mutant genes of Arabidopsis thaliana, which showed resistant behavior to artificial auxins under Dr. Nihal Dharmasiri funded by NIH, NSF, and DOE
TECHNIQUES, INSTRUMENTATION, AWARDS, VOLUNTEER
* Southern/Western Blotting techniques
* Mass spectrometry, GC-MS, HPLC
* SDS-PAGE Electrophoresis, Isoelectric Focusing
* Ion-Exchange Chromatography
* PCR
* Beckman Coulter: AU; 5400, 640, 680, UniCel DxI,
* Inova: QUNATA-Lyser
* BD Affirm : Micro Probe
* Otsuka America: POCOne-AS10
* Immucor Inc. : Neo-Galileo
* Siemens: Centaur
* FASEB MARC Award
* Dean`s List: Fall 2005, Spring 2006, Fall 2007, Spring 2008, Fall 2008
* Phi Theta Kappa (Gold Member)
* Who`s Who Among Students in American Junior Colleges
* St. Albert`s the Great Catholic church: CRHP Team 2 member Continuation Committee, & Eucharistic Minister
* Austin Samba School
* Austin Harmony Chorus
* St. Louis Catholic Church: Loaves and Fishes mobile food drive
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