Medical C
Resume:
CURRICULUM VITAE
Ambrose L. Cheung, M.D.
Department of Microbiology
Address:
Vail 206
Dartmouth Medical School
Hanover, NH 03755
Telephone:
Fax:
*******.******@*********.***
E-mail:
Citizenship: --b(6
B.A. Colby College, Waterville ME --b(6
Education:
M.D. Northwestern University Medical School 1976-1980
Postgraduate Training and Professional Experiences:
2002-present Professor of Microbiology, Dartmouth Medical School, Hanover, NH
1999- 2002 Associate Professor, Dartmouth Medical School, Hanover, N.H.
1996- 1999 Associate Professor, Rockefeller University, New York, N.Y.
1990 - 1995 Assistant Professor, Rockefeller University, New York. N.Y.
1986 - 1990 Research Associate, Rockefeller University, New York, N.Y.
1985 - 1986 Instructor, Harbor-UCLA Med. Ctr., UCLA School of Medicine, Los Angeles, CA
1983 - 1985 Fellow in Infectious Diseases, Harbor-UCLA Med. Ctr., UCLA School of
Medicine, Los Angeles, CA.
1981 - 1983 Resident in Internal Medicine, Harbor-UCLA Med. Ctr., UCLA School of
Medicine, Los Angeles, CA.
1980 - 1981 Intern, Harbor-UCLA Med. Ctr., UCLA School of Medicine, Los Angeles, CA.
Certification:
1986 Diplomate, American Board of Internal Medicine, Subspecialty in Infectious
Diseases
1983 Diplomate, American Board of Internal Medicine
Honor Society:
-b(6)- Phi Beta Kappa, Colby College
--b(6 Dana Scholar, Colby College
Award and Honors:
-b(6)- Departmental prize in Physics and Chemistry
1985 - 1986 National Research Service Award
1990 National Foundation For Infectious Diseases Young Investigator Matching Award
1990 - 1993 New York Heart Association Investigatorship
1994 - 1999 American Heart Association Established Investigatorship
1993 - 1998 Irma Hirschl Career Scientist Award
1993 - 1998 First Award from the National Institute of Health
2007- Honorary Professor, Dept of Microbiology, Faculty of Medicine, University of
Hong Kong
2007 AAAS Fellow
2009 Jun-Sept. Visiting Professor, Department of Medicine, University of Geneva. Switzerland.
Professional activities:
1991 - Pres. Adhoc Member, Small Business Innovative Research Study Section, National
Institute of Health
1986 - Pres. Member, American Society for Microbiology
1991 - Pres. Member of the Editorial Board, "Infection and Immunity"
1997 - 1999 Member of the Alumni Council, Colby College
1997 Member to selected Site Visit, NIH
1998 Adhoc member, MIDRC, NIAID, NIH
2001 Adhoc member, BM2 study section, NIH
2001 -2005 Regular Member, Study Section, Bacteriology and Mycology II, NIAID, NIH
2001 Member, site visit committee to National Institute of Child Health and Human
Development, NIH
2004 Organizing committee of the ISSSI in Charleston, SC
2005-2006 Adhoc member, HIBP study section, NIH
2006 Adhoc member, DDR study section, NIH
2007 Adhoc member, AED, study section, NIH
2007 Adhoc member, ZRG study section, NIH
2008 Adhoc member, ZRG study section, NIH
2008 Associate Editor, PloS Pathogen
2009 Member of the Faculty 1000 Biology
Committee responsibilities:
2005-2007 DMS Admission Committee
2007-present Academic Promotion Committee
2009 Member, search committee for the Chair of Pathology
2008 Member, search committee for recruitment of new immunology faculty member
Invited lectures:
May 1994 Convener and speaker at a symposium, "Global regulation and virulence
determinants in Staphylococcus aureus: In vitro and in vivo correlations" at the
Annual Meeting of the American Society for Microbiology.
Oct 1995 Invited speaker at the Gordon Conference on Staphylococci in Germany
"The sar regulatory locus"
Jun 1996 Invited speaker at the "8th International Symposium on Staphylococci and
Staphylococcal Infection" in Aix-Les-Bains, France on "The sar locus - a
pleiotropic regulator of virulence determinants in S. aureus"
Spring 1997 Departmental Seminar at the Microbiology Department at the University of
Kansas Regulation of virulence determinants by the sar locus
Spring 1997 Departmental Seminar at the Microbiology Department at the University of Illinois
in Chicago. Global regulatory locus sar
May 1998 Invited speaker on Bacterial Pathogenesis Seminar, University of Alabama at
Birmingham
October 1998 Invited speaker at the ASM regional meeting, Northeastern Branch, in Albany,
New York.
March 1999 Invited speaker at the 11th SB Kurhaus Workshop on Infectious Diseases,
Scheveningen, Holland
May 1999 Invited speaker at the Gordon Conference on Staphylococci in Barga, Italy
SarA binds to a consensus motif to activate target genes
Dec 1999 Departmental Seminar at the Department of Medicine, Albert Einstein College of
Medicine Regulation of virulence determinants in S. aureus: In vitro and in vivo
correlations
Mar 2000 Departmental Seminar at the Department of Molecular Genetics and
Microbiology at New Mexico School of Medicine, Virulence determinants of S.
aureus
June 2000 Invited speaker at the Gordon Conference in Microbial Toxin, Proctor Academy,
NH
Sep 2000 Invited speaker at the ICACC Conference on The role of SigB in S. aureus
infections at Toronto, Canada
March 2001 Invited speaker at the Grand Round, Division of Infectious Disease, Dartmouth
Hitchcock Medical Center, Hanover, NH.
Invited Speaker at the 3rd Annual Conference on Gram-Positive Cocci Infections
April 2001
in Humans in New York, NY
April 2001 Departmental Seminar at the Microbiology Department, Wake Forrest University
School of Medicine.
May 2001 Invited speaker at the symposium Recent development in Gram+ pathogenesis
at the Annual Meeting for the American Society for Microbiology in Orland, FL
May 2001 Departmental Seminar at the Infectious Disease Division, Department of
Medicine, Columbia College of Physicians and Surgeons.
June 2001 Invited speaker, at White River Junction VA hospital, at White River Junction,
Vermont.
Oct 2001 Invited Speaker at the departmental seminar, Department of Microbiology,
University of Vermont at Burlington
Invited Speaker at the 3rd Annual NARSA meeting (Network on Antimicrobial
March 2002
Resistance in Staphylococcus aureus). Myrtle Beach, SC.
April 2002 Microbiology Departmental Seminar at University of Idaho, Moscow
Invited speaker at the 10th ISSSI (International S. aureus meeting) meeting in
Oct 2002
Tsukaba, Japan
Nov 2002 Invited speaker at the Molecular Pathogenesis section, Yale School of Medicine
Nov 2002 Invited seminar speaker at Genome Therapeutic Corporation, Boston MA
May 2003 Convenor for an ASM symposium titled Winged helix proteins of the prokaryotic
and eukaryotic family
Sept 2003 Discussion leader on Virulence gene regulation in S. aureus at the Gordon
Conference in Oxford, England
Oct 2003 Invited speaker at the Departmental Seminar for Microbiology at Emory
University
Oct 2003 Invited speaker at the Departmental Seminar for Microbiology at University of
Minnesota
Nov 2003 Invited speaker at the Program for Microbiology at the University of Chicago
May 2004 Invited speaker at the Departmental Seminar of the Department of Microbiology
and Molecular Genetics at University of Massachusetts Medical School at
Worcester
Aug 2004 Invited speaker at the Departmental Seminar in the Department of Microbiology
at the University of Hiroshima, Hiroshima, Japan
Aug 2004 Invited speaker at the International Forum on Infection and Immunity, at Awaji
Island, near Osaka, Japan
Keynote address at the 11th ISSSI in Charleston, SC, USA.
Oct 2004
March 2005 Seminar speaker for NERCE, Harvard Medical School, Boston MA.
April 2005 Departmental seminar at National Jewish Hospital, Denver, CO.
Aug 2005 Invited speaker for the Gordon Conference on Staphylococcal Diseases
May 2006 Departmental Seminar, Microbiology Department, University of British Columbia,
Canada
June 2006 Departmental Seminar, University of Basel Hospital, Basel, Switzerland
Sept 2006 Departmental Seminar, University of Tuebingen, Tuebingen, Germany
Nov 2006 Departmental Seminar, University of Muenster, Muenster, Germany
Dec 2006 Departmental Seminar, Division of Biological Sciences, Nanyang University,
Singapore
April 2007 Departmental Seminar, University of Manchester, UK.
June 2007 Departmental Seminar, Department of Microbiology, Queen Mary Hospital,
University of Hong Kong
July 2007 Departmental Seminar, Microbial Pathogenesis, Genentech, South San
Francisco, CA.
Sept 2007 Departmental Seminar, Division of Infectious Diseases, University of Geneva
Hospital, Geneva, Switzerland.
April 2008 Departmental Seminar, Department of Microbiology, University of Swansea,
Swansea, Wales
Aug 2008 Departmental Seminar, Department of Molecular Genetics, University of
Brussels, Belgium
Oct 2008 Convener of a mini-lecture at the ICAAC/IDSA meeting in Washington, DC.
Dec 2008 Infectious Diseases Grand Round, Hitchcock Medical Center, Lebanon, NH
June 2009 Seminar, Department of Medicine, University of Geneva, Switzerland.
Oct 2009 Speaker, ISDA Annual Meeting, Philadelphia, PA.
Sept 2010 Speaker, ISSSI (International Staphylococcal meeting), in Bath, England
Publications:
1. Cheung, A.L. and V. A. Fischetti. 1988. Variation in the expression of cell wall proteins of
Staphylococcus aureus grown on solid and liquid media. Infect. Immun. 56:1061-1065.
2. Cheung, A.L., A. S. Bayer, J. Peters, and J. I. Ward. 1987. Analysis by gel electrophoresis,
western blot and peptide mapping of protein A heterogeneity in Staphylococcus aureus strains.
Infect. Immun. 55:843-847.
3. Cheung, A.L., A. S. Bayer, J. Peter, and J. I. Ward. 1988. Surface proteins of
Staphylococcus aureus. Rev. Infect. Dis. 10 Suppl.2:S351-S355.
4. Greenberg, D.P., A. S. Bayer, A. L. Cheung, and J. I. Ward. 1989. Protective efficacy of
protein A-specific antibody against bacteremic infection due to Staphylococcus aureus in an
infant rat model. Infect. Immun. 57:1113-1118.
5. Cheung, A.L. and V. A. Fischetti. 1989. The role of surface proteins in staphylococcal
adherence to fibers in vitro. J. Clin. Invest. 83:2041-2049.
6. Cheung, A.L., P. Ying, and V. A. Fischetti. 1991. A method to detect proteinase activity using
unprocessed X-ray films. Anal. Biochem. 193:20-23.
7. Cheung, A.L. and V. A. Fischetti. 1991. The role of fibrinogen in mediating staphylococcal
adherence to fibers. J. Surg. Res. 50:150-155.
8. Cheung, A.L. and V. A. Fischetti. 1990. The role of fibrinogen in staphylococcal adherence to
catheters in vitro. J. Infect. Dis. 161:1177-1186.
9. Cheung, A.L., M. Krishnan, E. A. Jaffe, and V. A. Fischetti. 1991. Fibrinogen acts as a
bridging molecule in the adherence of Staphylococcus aureus to cultured human endothelial
cells. J. Clin. Invest. 87:2236-2245.
10. Cheung, A.L., J. M. Koomey, S. Lee, E. A. Jaffe, and V. A. Fischetti. 1991. Recombinant
human tumor necrosis factor alpha promotes adherence of Staphylococcus aureus to
endothelial cells. Infect. Immun. 59:3827-3831.
11. Cheung, A.L., J. M. Koomey, C. A. Butler, S. J. Projan, and V. A. Fischetti. 1992. Regulation
of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr. Proc. Natl.
Acad. Sci. USA. 89:6462-6466.
12. Cheung, A.L., M. Yeaman, and A. S. Bayer. 1994. The role of the sar locus in the induction
of experimental endocarditis in rabbits. Infect. Immun. 62:1719-1725.
13. Cheung, A.L. and P. Ying. 1994. Regulation of and hemolysins by the sar locus of S.
aureus. J. Bacteriol. 176:580-585.
14. Cheung, A.L. and S. J. Projan. 1994. Cloning and sequencing of sarA: a gene required for
the expression of agr. J. Bacteriol. 176:4168-4172.
15. Ren, K., J. D. Bannan, V. Pancholi, A. L. Cheung, J. C. Robbins, V. A. Fischetti, and J. B.
Zabriskie. 1994. Purification, characterization and biological properties of a new toxin from S.
aureus inducing TSS-like illness in rabbits - J. Exp. Med. 180:1675-1683.
16. Cheung, A.L., K. Eberhardt, E. Chung, M. Yeaman, P. Sullam, M. Ramos, and A. S. Bayer.
1994. Diminished virulence of a sar-/agr- mutant of Staphylococcus aureus in the rabbit model of
endocarditis. J. Clin. Invest. 94:1815-1822.
17. Cheung, A. L., K. Eberhardt and V. A. Fischetti. 1994. A method to isolate RNA from gram-
positive bacteria and mycobacteria. Anal. Biochem. 222:511-514.
18. Lo, S. K., A. L. Cheung, Q. Zheng, and R. L. Silverstein. 1995. Induction of tissue factor on
monocytes by adhesion to endothelial cells. J. Immunol. 154:4768-4777.
19. Cheung, A. L., C. Wolz, M. R. Yeaman, A. S. Bayer. 1995. Insertional inactivation of a
chromosomal locus that modulates expression of potential virulence determinants in
Staphylococcus aureus. J. Bacteriol. 177:3220-3226.
20. Cheung, A.L., S. J. Projan, R. Edelstein, and V. A. Fischetti. 1995. Cloning, expression and
nucleotide sequencing of a S. aureus gene (fbpA) encoding a fibrinogen binding protein. Infect.
Immun. 63:1914-1920.
21. Dana, R.C., M. Saghbini, D. Lippman, and A. L. Cheung. 1995. Isolating RNA using the
new FastPrep system. J. NIH Res. 7:61.
22. Bayer, A. S., P. M. Sullam, M. Ramos, C. Li, A. L. Cheung, and M. R. Yeaman. 1995.
Staphylococcus aureus induces platelet aggregation via a fibrinogen-dependent mechanism
which is independent of principal platelet GP IIb/IIIa fibrinogen-binding domains. Infect. Immun.
63:3634-3641.
23. Heinrich, J. H., M. G. Bayer, and A.L. Cheung. 1996. Characterization of the sar locus and
its interaction with agr in S. aureus. J. Bacteriol. 178:418-423.
24. Bayer, M. G., J. H. Heinrichs, and A. L. Cheung. 1996. Molecular architecture of the sar
locus.
J. Bacteriol. 178:4563-4570.
25. Wolz, C., D. McDevitt, T. J. Foster, and A. L. Cheung. 1996. The influence of agr on
fibrinogen binding in S. arueus Newman. Infect. Immun. 64:3142-3147.
26. Sullam, P.S., A. S. Bayer, W. M. Foss, and A. L. Cheung. 1996. Diminished platelet binding
in vitro by S. aureus is assocaited with reduced virulence in a rabbit model of infective
endocarditis. Infect. Immun. 64:4915-4921.
27. Nilsson,I., T. Bremell, C. Ryd n, A. L. Cheung, and A. Tarkowski. 1996. The role of
staphylococcal accessory regulator (sar) in septic arthritis. Infect. Immun. 64:4438-4443.
28. Booth, M.C., A. L. Cheung, H. Hatter, and M. S. Gilmore. 1997. Sar contributes to
Staphylococcus aureus virulence in endophthalmitis only in conjunction with agr. Infect. Immun.
65:1550-1556.
29. Liu, S., S. Sela, G. Cohen, J. Jadoun, A. L. Cheung, and I. Ofek. 1997. Genetic evidence for
co-regulation of Streptolysin S production and riboflavin biosynthesis in S. pyogenes. Microbiol.
Pathog. 22:227-234.
30. Cheung, A.L., K. Eberhardt, and J. H. Heinrichs. 1997. Regulation of protein A by the sar
and agr loci of Staphylococcus aureus. Infect. Immun. 65:2243-2249.
31. Cheung, A.L., M. G. Bayer, and J. H. Heinrichs. 1997. The genetic requirement of sar in the
expression of RNAII and RNAIII in Staphylococcus aureus. J. Bacteriol. 197:3963-3971.
32. Dhawan, V., M. R. Yeaman, A. L. Cheung, E. Kim, P. A. Sullam, and A. S. Bayer. 1997.
Phenotypic resistance to thrombin-induced platelet microbicidal protein in vitro is correlated with
enhanced virulence in experimental endocarditis due to Staphylococcus aureus. Infect. Immun.
65:3293-3299.
33. Giraudo, A.T., A. L. Cheung and R. Nagel. 1997. The sae locus of Staphylococcus aureus
controls exoprotein synthesis at the transcriptional level. Arch. Microbiol. 168:53-58.
34. Bayer, A. S., M. D. Ramos, B. Menzies, M. R. Yeamn, A. Shen and A. L. Cheung. 1997.
Hyperproduction of alpha-toxin by Staphylococcus aureus results in paradoxically reduced
virulence in experimental endocarditis: a host-defense role for platelet microbicidal proteins.
Infect. Immun. 65:4652-4660.
35. Chien Y. T., and A. L. Cheung. 1998. Molecular interactions between two global regulators,
sar and agr in Staphylococcus aureus. J. Biol. Chem. 273:2645-2652
36. Manna, A., M. G. Bayer, and A. L. Cheung. 1998. Transcriptional analysis of different sar
promoters in Staphylococcus aureus. J. Bacteriol. 180:3828-3836.
37. Gillaspy, A.F., C.Y. Lee, S. Sau, A. L. Cheung, and M. S. Smeltzer. 1998. Factors affecting
the collagen binding capacity of Staphylococcus aureus. Infect. Immun. 66:3170-3178.
38. Fluckiger, U., and A. L. Cheung. 1998. Charcterization of a sar homolog of S. epidermidis.
Infect. Immun. 66:2871-2878.
39. Chien, Y. T., and A. L. Cheung. 1998. SarA level is a determinant of agr activation in
Staphylococcus aureus. Mol. Microbiol. 31:991-1011.
40. Cheung, A. L., C. C. Nast, and A. S. Bayer. 1998. Selective activation of sar promoters of S.
aureus in the rabbit endocarditis model using Green Fluorescent Protein (gfpuv) transcription
fusion as a detection system. Infect. Immun. 66:5988-5993.
41. Cheung, A. L., Y.-T. Chien and A. S. Bayer. 1999. Hyperproduction of alpha hemolysin in a
sigB mutant is associated with elevated SarA expression in Staphylococcus aureus. Infect.
Immun. 67:1331-1337.
42. Y.T. Chien, A.C. Manna, S.J. Projan, and A.L. Cheung. 1999. SarA, a global regulator of
virulence determinants in Staphylococcus aureus, binds to a conserved motif essential for sar
dependent gene regulation. J. Biol.Chem. 274:371**-*****.
43. Wolz, C., A. Steinhuber, P. P hlmann-Dietze, Y. T. Chien, A.C. Mann, W. Van Wamel and
A.L. Cheung. 2000. Agr-independent regulation of fibronectin binding proteins by the regulatory
locus sar in Staphylococcus aureus. Mol. Microbiol. 36:230-243.
44. Gresham, H. D., J. H. Lawrence, T. E. Caver, Bridget S. Wilson, A. L. Cheung, and F.P.
Lindberg. 2000. Survival of Staphylococcus aureus inside neutrophils contributes to infection. J.
Immunol. 164:3713-3722.
45. Kahl, B.C., M. Goulian, W. Van Wamel, M. Herrmann, S. Simon, G. Kaplan, G. Peters, and
A.L. Cheung. 2000. Staphylococcus aureus RN6390 replicates and induces apoptosis in a
pulmonary epithelial cell line derived from a cystic fibrosis patient. Infect. Immun. 68:5385-5392.
46. A.C. Manna, and A.L. Cheung. 2001. Characterization of sarR, a modulator of sar
expression in Staphylococcus aureus. Infect.Immun. 69:885-896.
47. A. L. Cheung, K. Schmidt, B. Bateman, and A. C. Manna. 2001. SarS, a SarA homolog
repressible by agr, is an activator of protein A synthesis in Staphylococcus aureus.
Infect.Immun. 69:2448-2455.
48. Y. Liu, R. Li, R. C. Murphy, A. L. Cheung and G. Zhang. 2001. Crystal structure of the SarR
protein from Staphylococcus aureus. PNAS. 98:6877-6882.
49. I. Siboo, A. L. Cheung, A.S. Bayer and P. Sullam. 2001. Clumping factor mediates binding
of Staphylococcus aureus to human platelets. Infect.Immun. 69:3120-3127.
50. A. Ratner, R Bryan, B. Nguyen, D. Barnes, A. Weber, A. Pitt, S. Gelber, A. Cheung, A.
Prince. 2001. Cystic fibrosis pathogens activate epithelial cells through Ca 2+-dependent MAPK
signaling pathways. J.Biol. Chem. 276:192**-*****
51. K. A. Schmidt, A. Manna, S. Gill, and A.L. Cheung. 2001. SarT: a repressor of -hemolysin
synthesis in S. aureus. Infect.Immun. 69:4749-4758.
52. T. Kielian, A. Cheung, and W.F. Hickey. 2001. Diminished virulence of an alpha toxin mutant
of Staphylococcus aureus in experimental brain abscesses. Infect. Immun. 69:6902-6911.
53. B. Shenkman, E. Rubenstein, A. L. Cheung, G.E. Brill, R. Darkik, I. Tamarin, N. Savion and
D. Varon. 2001. Adherence properties of Staphylococcus aureus under static and flow
conditions: role of agr and sar loci, platelets and plasma ligands. Infect. Immun.69:4473-4478.
54. A. L. Cheung, and Gongyi Zhang. 2001. Do SarA and SarR possess similar structures?
Trends in Microbiology. 9:570-573.
55. M. Palma, and A. L. Cheung. 2001. B activity in Staphylococcus aureus is controlled by
RsbU and additional factor(s) during growth. Infect. Immun.69:7858-7865.
56. B.Y. Bateman, N. Donegan, T. Jarry, M. Palma and A.L. Cheung. 2001. Evaluation of a
tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo, and its application
in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857
57. G. Heyer, A. Pitt, S. Saba, D. Barnes, A. Weber, W. Rush, S. Nguyen, R. Bryan, A. Cheung
and A. Prince. 2002. Role of agr and sar dependent gene products in the pathogenesis of
Staphylococcus aureus pulmonary infection. Infect. Immun.70:127-133.
58. Cheung, A.L. and Zhang, G. 2002. Global regulation of virulence determinants in S. aureus
by the SarA protein family. Front. Sci. 7:d1825-1842.
59. W. Van Wamel, Y.-Q. Xiong, M.R. Yeaman, C.C. Nast, A.S. Bayer and A.L. Cheung. 2002.
Regulation of cap5 promoter activity agr and sarA in vitro and in vivo. Microb. Pathog. 33:73-79.
60. Y.-Q. Xiong, W. Van Wamel, C.C. Nast, M.R. Yeaman, A.L. Cheung and A.S. Bayer. 2002.
Activation and transcriptional interaction between two S. aureus agr RNA molecules in an
experimental endocarditis model. J. Infect. Dis. 186:668-77.
61. Cheung, A.L., S.J. Projan, and H. Gresham. 2002. The genomic aspect of virulence, sepsis
and resistance to killing mechanisms in S. aureus. Curr. Infect. Dis. Reports. 4:400-410.
62. Manna, A.C. and A.L. Cheung. sarU, a gene repressible by sarT, is an activator of RNAIII in
S. aureus. Infect. Immun. 2003. Infect. Immun. 71:343-353.
63. Kupferwasser, I. L., M.R. Yeaman, C. C. Nast, D. Kupferwasser, A. L. Cheung, A. S. Bayer.
2003. Salicylic Acid Attenuates Virulence in Endovascular Infections by Targeting Global
Regulatory Pathways in Staphylococcus aureus. J. Clin. Invest. 110:222-233.
64. Ingavale, S.S., W. Van Wamel, and A.L. Cheung. 2003. Characterization of Rat, an
important autolysis regulator in Staphylococcus aureus. Mol. Microbiol. 48:1451-1466.
65. Li, R., A.C. Manna, A.L. Cheung, and G. Zhang. 2003. The crystal structure of the SarS
protein from Staphylococcus aureus. J. Bacteriol. 185:4219-4225.
66. K. A. Schmidt, A.C. Manna and A.L. Cheung. 2003. SarT influences sarS expression in
Staphylococcus aureus. Infect. Immun. 71:5139-5148.
67. Jacob M. Rothfork. Sophie Dessus-Babus, Willem Van Wamel, Ambrose L. Cheung & Hattie
D. Gresham. 2003. J. Immunol. Host potentiation of bacterial quorum sensing during
Staphylococcus aureus infection. 171:5389-5395.
68. Cheung, A.L., Bayer, A.S., Zhang, G., Gresham, H., and Xiong, Y.-Q. 2004. Regulation of
virulence determinants in vitro and in vivo in Staphylococcus aureus. FEMS
Microbiology/Immunology. 1649:1-9.
69. Bisognano, C., Kelley,W., Francois, P., Schrenzel, J., Li, D. Estoppey, T., Lew, D.P.,
Hooper, D.C., Cheung, A.L. and Vaudaux, P. A. 2004. RecA-dependent pathway mediates
ciprofloxacin-induced fibronectin-binding proteins in S. aureus. J. Biol. Chem. 279:9064-9071
70. Y.-Q. Xiong, A.S. Bayer, M.R. Yeaman, W. Van Wamel, A.L. Cheung. 2004. Impacts of sarA
and agr in Staphylococcus aureus upon Fibronectin-Binding Protein A gene expression and
fibronectin adherence capacity in vitro and in experimental infective endocarditis. Infect.
Immun. 72:1832-1836.
71. K. A. Schmidt, N. P. Donegan, W. A. Kwan, Jr. and A.L. Cheung. 2004. B influences
expression of staphylococcal enterotoxins B in S. aureus. Can. J. Microbiol. 50:351-360.
72. A. C. Manna, S.S. Ingavale, M. Maloney, W. van Wamel, and A.L. Cheung. 2004.
Characterization of sarV, a transcriptional regulator of the sarA family, is repressed by SarA and
Rat and regulates autolysis and virulence genes in Staphylococcus aureus. J. Bacteriol.
186:5267-5280
73. M. Gomez, A. Lee, B. Reddy, A. Muire, G. Soong, A. Pitt, A. L. Cheung, and A. Prince.
2004. S. aureus protein A induces airway epithelial inflammatory responses by triggering
TNFR1 and mimicking TNF- activity. Nature Medicine. 10:842-848.
74. J. M. Rothfork, G. Timmins, M. N. Harris, X. Chen, A. L. Lusis, M. Otto, A. L. Cheung and H.
D. Hattie. 2004. Inactivation of a bacterial virulence pheromone by phagocyte-derived oxidants:
new role for the NADPH oxidase in host defense. PNAS. 101:138**-*****.
75. Ingavale, S., van Wamel, W., Luong, T., Lee. C.Y., and Cheung, A.L. 2005. Rat/MgrA, a
regulator of autolysis, is a regulator of virulence genes in Staphylococcus aureus. Infect. Immun.
73:1423-1431.
76. Tormo, M.A., Marti, M., Valle, J., Manna, A.C., Cheung, A.L., Lasa,I., and Penades, J.R. 2005.
SarA is an essential positive regulator of Staphylococcus epidermidis biofilm development. J.
Bacteriol. 187:2348-2356.
77. Cheung, A. L. and Manna, A.C. 2005. The role of the distal sarA promoters in SarA
expression in Staphylococcus aureus. Infect. Immun. 73:4391-4394
78. G. Belling, A. L. Cheung, G. Peters, M. Herrmann and B. Kahl. 2005. Thymidine-dependent
Staphylococcus aureus small colony variants (SCV) are associated with dramatic changes in
regulator and virulence gene expression. Infect. Immun.. 734***-****.
79. B. Haslinger-L ffler, Kahl, B.C., Strangfeld, K., Grundmeier, M., WagneR. B, Fischer. U.,
Cheung, A.L., Peters, G., Schulze-Osthoff, K., and Sinha, B. 2005. Multiple virulence factors
are required for Staphylococcus aureus-induced apoptosis in endothelial cells. Cell. Microbiol.
7:1087-1097.
80. R. M. Shanks, N. P. Donegan, M. L. Graber, S. A. Buckingham, M. E, Zegans, A. L.
Cheung, and G. A. O Toole. 2005. Heparin stimulates S. aureus biofilm formation. Infect.
Immun. 73:4596-4606.
81. M. P. Trotonda, A. C. Manna, A. L. Cheung, I. Lasa, J. R. Penades. 2005. SarA controls
Bap-dependent biofilm formation in Staphylococcus aureus. J. Bacteriol. 187:5790-5798.
82. A. S. Bayer, P. McNamara, M. R. Yeaman, N. Lucindo, T. Jones, A. L. Cheung, H.-G. Sahl,
and R. A. Proctor. 2006. Transposon Disruption of the Complex I NADH Oxido-Reductase
Gene (snoD) in Staphylococcus aureus is Associated with Reduced Susceptibility to the
Microbicidal Activity of Thrombin-induced Platelet Microbicidal Protein-1. J. Bacteriol. 188:211
222.
83. Y. Liu, A.C. Manna, C.-H. Pan, I. A. Kriksunov, D. J. Thiel, A. L. Cheung, and G. Zhang.
2006. Structure and function analyses of the global regulatory protein SarA from S. aureus.
PNAS.103:2392-2397.
84. L. S. Miller, R. M. O Connell, M. A. Gutierrez, A. Shahangian, C. E. Gross, J. Thirumala, A.
L. Cheung, G. Cheng and R. L. Modlin. 2006. IL-1R/MyD88 signaling by resident skin cells is
required for immunity against Staphylococcus aureus. Immunity. 24:79-91.
85. T. Jarry and A. L. Cheung. 2006. Staphylococcus aureus escapes more efficiently from the
phagosome of a Cystic Fibrosis bronchial epithelial cell line than its normal counterpart. Infect.
Immun. 74:2568-2577.
86. A. C. Manna and A. L. Cheung. 2006. Mutational analysis of SarR, a winged helix protein of
the SarA protein family that is involved in the regulation of the agr locus. Mol. Microbiol.
60:1289-1901.
87. A. C. Manna and A. L. Cheung. 2006. Characterization of SarX, a negative regulator of agr
and exoprotein synthesis, is activated by MgrA in S. aureus. J. Bacteriol. 188:4288-4299.
88. Yan Q. Xiong, Julie Willard, Cynthia C. Nast, Michael R. Yeaman, Ambrose L. Cheung and
Arnold S. Bayer. 2006. Regulation of Staphylococcus aureus -toxin protein and gene (hla)
expression by agr, sarA and sae in vitro and in Experimental Infective Endocarditis. JID. 194:
1267-1275.
89. M. Palma, A. S. Bayer, L. I. Kupferwasser, T. Yoska, M. R. Yeaman and A.L. Cheung. 2006.
Salicylic acid activates sigma factor B by rsbU-dependent and independent mechanisms. J.
Bacteriol. 188:5986-5903.
90. M. Sedlacek, Gemery, J.M., Cheung, A.L., Bayer, A.S. and Remillard, B.D. 2007. Lower
Incidence of Staphylococcus Aureus Bacteremia in Patients with Tunneled Dialysis Catheters
treated with Aspirin. Amer. J. Digest. Kidney Dis. 49:401-408.
91. M. Meehl, Herbert, S., G tz, F. and Cheung, A. L. 2007. Interaction of the GraRS two-
component system with the VraFG ABC-transporter to support vancomycin-intermediate
resistance in Staphylococcus aureus. Antimicrob. Agents. Chemo. 51:2679-2689.
92. T. Kielian, N. Esen, A. Haney, N.K. Phulwani, P.M. Hayes, A.L. Cheung and J.J. Ruhe.
2007. Minocycline modulates neuroinflammation independent of its antimicrobial activity in S.
aureus-induced brain abscess. 2007. Amer. J. Path. 171:1199-1214.
93. Martina Ulrich, Mike Bastian, Katrin Ziegler, Sarah E. Cramton, Alexa A. Pragman,
Alessandra Bragonzi, Guido Memmi, Christiane Wolz, Patrick M. Schlievert, Ambrose Cheung
and Gerd D ring. 2007. The staphylococcal respiratory response regulator SrrAB induces ica
gene transcription and PIA expression, protecting Staphylococcus aureus from neutrophil killing
under anaerobic growth conditions. Mol. Microbiol. 65:1276-1287.
94. Silvia Herbert, Agnieszka Bera, Christiane Nerz, Dirk Kraus, Andreas Peschel, Christiane
Goerke, Michael Meehl, Ambrose Cheung, Friedrich G tz. 2007. Molecular basis of resistance
to lysozyme's muramidase and antimicrobial peptide activity in staphylococci. Plos Pathogens.
3:981-994.
95. Miller, L.S., Pietras, E.M., Uricchio, L. H., Hirano, K., Rao, S., Lin, H., O Connell, R. M.,
Iwakura, Y., Cheung, A.L., Cheng, G., and Modlin, R.L. 2007. Inflammasone-mediated
production of Il-1 is required for neutrophil recruitment against Staphylococcus aureus. J.
Immunol. 179:6933-42
96. Zhibiao Fu, Niles Donegon, Guido Memmi and Ambrose L Cheung. 2007. Characterization
of MazF-sa, an endoribonuclease from Staphylococcus aureus. J. Bacteriol. 189:8871-8879.
97. Cheung, A.L., K. Nishina, M. P. Trotonda and S. Tamber. 2008. Review of the SarA protein
family. International Journal of Biochemistry and Cell Biology. 40:355-361.
98. Min-Ho Kim, Wei Liu, Dori L. Borjesson, Fitz-Roy E. Curry, Lloyd S. Miller, Ambrose L.
Cheung, Fu-Tong Liu, R.R. Isseroff and Scott I Simon. 2008. Dynamics of neutrophil infiltration
during cutaneous wound healing and infection using fluorescence imaging. J.Invest.Dermatol.
128:1812-20.
99. Dongmei Li and Cheung, A.L. 2008. The repression of hla by rot is dependent on sae in
Staphylococcus aureus. Infect. Immun. 76:1068-1075.
100. Cheung, A.L., K. Nishina, and A. C. Manna. 2008. SarA binds to its own promoter to
regulate gene expression. J. Bacteriol. 190:2239-2243.
101. Robert M. Q. Shanks, Michael Meehl, Kimberly M. Brothers, Raquel M. Martinez, Niles P.
Donegan, Martha L. Graber, Ambrose L. Cheung and George A. O Toole. 2008. The Alternative
citrate-dependent biofilm formation pathway of Staphylococcus aureus is dependent on
fibronectin binding proteins and the GraRS two-component regulatory system. Infect. Immun.
76:2469-2477.
102. Jarry, T. and Cheung, A.L. 2008. The expression of alpha hemolysin is sufficient for S.
aureus phagosomal escape after internalization in CFT-1 cells. Cellular Microbiology. 10:1801
1814
103. Guido Memmi, Sergio R. Filipe, Mariana G. Pinho, Ambrose Cheung. 2008.
Staphylococcus aureus PBP4 is essential for -lactams Resistance in Community Acquired
Methicillin Resistant Strains. Antimicro. Agents Chemo. 52:3955-3966.
104. Maria P. Trotonda, Sandeep Tamber, Guido Memmi and Ambrose L. Cheung. 2008. MgrA
represses biofilm formation in Staphylococcus aureus. Infect. Immun. 76: 5645 5654.
105. Maren von K ckritz-Blickwede, Manfred Rohde, Sonja Oehmcke, Lloyd S. Miller, Ambrose
L. Cheung, Heiko Herwald, Simon Foster, and Eva Medina. 2008. Immunological Mechanisms
Underlying the Genetic Predisposition to Severe Staphylococcus aureus Infection in the Mouse
Model. American Journal of Pathology. 173(6):1657-68.
106. M. Michal Peterson, Jessica L. Mack, Anny A. Alsup, Susan M. Alexander, Youhanna S.
Sawires, Aldons J. Lusis, Ambrose L. Cheung, and Hattie D. Gresham. 2008. Apolipoprotein B
is an Innate Barrier Against Invasive Staphylococcus aureus Infection. Cell Host and Microbes.
4(6):555-566.
107. Tamber, S. and A. L. Cheung. 2009. SarZ promotes the expression of virulence factors
and represses biofilm formation by modulating SarA and agr in Staphylococcus aureus. Infect.
Immun. 77: 419 428
108. Mar a Pilar Trotonda, Yan Q. Xiong, Arnold S. Bayer and Ambrose L. Cheung. 2009. The
role of mgrA and sarA in autolysis and resistance to cell-wall active antibiotics in methicillin
resistant S. aureus. J. Infect. Dis. 199:209-18.
109. Fu, Z., S. Tamber, G. Memmi, N. P. Donegan and A. L. Cheung. 2009. Over-expression of
MazFsa induces bacteriostasis with essential mRNA protected in Staphylococcus aureus. J.
Bacteriol. 191:2051-2059.
110. Niles P. Donegan and Ambrose L. Cheung. 2009. Regulation of the mazEF toxin-antitoxin
module in Staphylococcus aureus and its impact on sigB expression. J. Bacteriol. 191:2795
2805.
111. Lauderdale, K. J., B. R. Boles, A. L. Cheung, and A. R. Horswill. 2009. Interconnection
between Sigma B, agr and proteolytic activity in Staphylococcus aureus biofilm maturation.
Infect. Immun. 77: 1623 1635.
b(4
113. Ambrose L. Cheung, Soo-Jing Yang, Arnold S. Bayer and Yan Q. Xiong. 2009. Disparity In
the in vitro vs. in vivo regulation of fibronectin-binding proteins (FnBPs) by saeRS and/or sigB,
two global regulators of virulence determinants, in Staphylococcus aureus. JID. 200:1371-1374.
---b(4
b(4
Manuscripts submitted or in the process of submission:
--b(4
---b(4
b(4
b(4
b(4
b(4
Book Chapter:
1. Global regulation of virulence determinants in S. aureus, in Staphylococcal Disease and
Infection edited by A. Honeyman, H. Friedman and M. Bendinelli, Kluwer Academic/Plenum
Publisher, New York. 2001
2. Regulatory of virulence determinants by the SarA protein family in S. aureus. Edited by Peter
Zuber. In Frontiers in Bioscience. 2002.
3. Tissue culture assays used to analyze invasion by Staphylococcus aureus, in Current
Protocol in Microbiology edited by, John Wiley & Sons, Inc, New York. p. 9C.4.1-9C.4.7, 2007.
4. Isolation and culture of human umbilical vein endothelial cells (HUVEC), in Current Protocol
in Microbiology edited by, John Wiley & Sons, Inc, New York. p. A.4B.1-A.4B.8, 2007.
Teaching
Course director Molecular Pathogenesis Journal Club MI265
Instructor Medical Microbiology Course for Medical Students at Dartmouth Med
School
Course director Microbiology/Immunology 148 - Courses in Advanced Molecular
Pathogenesis
Instructor Core lecture for the MCB program (Microbiology/Immunology) MI102
Mentoring for undergraduates
[ ]
b(6)
Former graduate students who matriculated with Ph.D.
[] b(6)
Current graduate students
[]b(6)
Former postdoctoral fellows
[ ]
b(6)
b(6
Active Grants
Agency Title Direct amount Beginning/end dates
1. NIH RO1 "Characterization of sar/agr $200,000/yr 7/06 to 6/11
interactions in S. aureus
2. R21 Defining resistance determinants $125,000 2/1/09-1/31/11
of CA-MRSA
3. 2 year ARRA award
The toxin-antitoxin system of $200,000 7/1/09 6/30/2011
Staphylococcus aureus
Patents:
1. Process for isolating cellular components including RNA: US patent 5643767
2. Compositions and methods for affecting virulence determinants in bacteria: US patent
7285995
3. Regulation of exoproteins in S. aureus: US patent 5976792
4. Compositions and methods for regulating autolytic processes in bacteria: US patent 6929913
Pending:
[ ]
b(6)
Contact this candidate