Project Medical
Resume:
L ung-Ji C hang
Name Lung-Ji Chang
e-mail abpwwi@r.postjobfree.com
Internet link http://myprofile.cos.com/lchang
Birth October 14, 1958
Citizenship US
Marital Status Married with one child
Education
1980 B.S. National Taiwan University
1986 Ph.D. University of Iowa
Government Service
1980-1982 Mandatory military service, Taiwan
Employment
1986-1989 Postdoctoral (Supervisor: Harold Varmus, Nobel Laureate - 1989)
Department of Microbiology and Immunology
University of California, San Francisco
1990-1993 Visiting Associate
Laboratory of Molecular Microbiology
National Institutes of Allergy and Infectious Diseases
National Institutes of Health, Bethesda, Maryland
1993-1997 Assistant - Associate Professor (tenured)
Department of Medical Microbiology & Immunology
University of Alberta, Edmonton, Alberta
Division of Infectious Disease, Department of Medicine
University of Alberta, Edmonton, Alberta
1997-Present Associate Professor - Professor
Department of Molecular Genetics and Microbiology, Powell Gene
Therapy Center, McKnight Brain Institute, Shands Cancer Center,
University of Florida, Gainesville, Florida
Research Grants and Funds Received (completed and active)
AHFMR (Alberta Heritage Foundation for Medical Research) $200,000-****-****
Project title: Isolation of human cDNA encoding for the HIV attachment and
penetration factors
MRC (Medical Research Council) $ 210,750-****-****
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Project title: Characterization of human host factors necessary for HIV attachment and
penetration .
ANR Trust Fund $ 5,000-****-****
Project title: Molecular design and testing of an anti-HIV mega-ribozyme
NHRDP (AIDS Grant) $ 285,000-****-****.
Project title: Development of retroviral delivery system for anti-HIV gene therapy .
Cancer Research Funds (Central Research & Cross Cancer Institute's Special Funds)
$ 50,000 1995
Project title: Anti-tumor immuno-gene therapy .
ANR Trust Fund $5,000-****-****
Project title: Study of HIV-specific cellular immune response in HIV-seronegative
high-risk individuals .
Alberta Cancer Board, Research Initiative Program Director $200,000/yr 1996-1998
Project title: Anti-cancer gene therapy: development of hu-PBL-SCID mouse human
tumor models and assessment of combined immuno-gene therapeutic effects
Briana Biotech Inc., Research Contract $60,000/yr 1997
Project title: Combination immunogene therapy for brain and skin cancer
National Institutes of Health, R21-NIDDK (awarded, returned due to overlap)
$100,000/yr 1997-1999
Project title: Development of lentiviral vectors .
University of Florida, College Investment Fund Grant $20,000 1999
Project title: Comparison of transduction efficiencies for CD34+ hematopoietic
stem cells by the amphotropic Moloney murine leukemia virus (MLV) and the HIV
based lentiviral vector system .
Elsa Pardee Foundation (Co-investigator, 5%, PI: Dr. J. R. Zucali) $97,986/yr 1999
Project title: Lentiviral gene transfer in human hemopoietic stem cells
NIH, NHLBI (subproject 4 PI, PPG Director Muzyczka) $137,000/yr 1997-2002
Project title: Development of lentiviral vectors .
National Institutes of Health, RO3 PI $50,000/yr 1999-2003
Project title: HIV-1 Vaccine and Immunity Studies in Hu-PBL SCID/beige Mice .
AMDL Inc., Breast Cancer Research (Tustin, California) $68,750-****-****
Project title: Combination immunogene therapy for breast cancer
UF sponsored research, Co-PI (PI, Dr. Rowan Milner) $50,000-****-****
Project title: In Vitro Analysis of Anti-Feline Vaccine-Associated Sarcoma (FVS)
Immunity of Immunogene Modified FVS Vaccine
Multiple Myeloma Research Foundation 2004 Senior Researcher Award
$100,000/yr 2004-2005
Project title: Dendritic cell and T cell therapy targeting multiple myeloma-specific
antigens
NIH, R21 Co-PI (PI, Yang, L.-J.) $100,000/yr 2004-2006
Project title: Transdifferentiation of hepatocytes into insulin-producing cells by
genetic modifications
National Institutes of Health, NHLBI (subproject 3 PI, PPG Director Muzyczka)
$240,000/yr 2002-2008
Project title: Improvement of safety, efficacy and in vivo models of lentiviral
vectors
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NIH R21 DK 68031 Co-PI (PI: Yang, L.J.) $100,000/yr 2005-2007
Project title: Autoimmunity of hepatocyte-derived insulin-producing cells
NIH R01 DK071831 Co-PI (PI: Yang, L.J.) $1,732,350-****-****
Project title: Liver to Endocrine Transdifferentiation
NIH R01 HD37432 Co-PI (PI: Chengini, N.) $191,500/yr 2005-2010
Project title: Molecular mechanism of leiomyoma growth and regression
VBI $700,000-****-****
Project title: Immunotherapy for leukemia
VBI $800,000-****-****
Project title: Immunotherapy for cancer
UF/Moffitt Cancer Center Collaborative Initiative Co-PI (PI: Sheng, W.)
$100,000/yr 2009-2010
Project title: Target Identification of Lenalidomide in del5q MDS
UF/Moffitt Cancer Center Fall 2008 Competition PI $75,000/yr 2009-2011
Project title: Immune cell therapy targeting multiple myeloma
Training Grants:
NIH - Microbiology and Infectious Diseases
NCI - Cancer cell biology
Pending
NIH RO1 Safety and efficacy evaluation of an advanced lentiviral vector system .
Responsibility: PI
Honor/Awards
1977-1980 Tuition Award, National Taiwan University
1978-1980 Book Coupon Award (1978, 1979, 1980)
1979 Matsumoto Scholar, National Taiwan University
1981 Commissioned Officer Award ($2,000 NTD)
1982-1986 Teaching Assistance Scholarship, University of Iowa
1986-1989 Postdoctoral Fellowship, University of California, San Francisco
1990-1993 Fogarty Visiting Associate, National Institutes of Health
1993-1998 Research Scholar, Alberta Heritage Foundation for Medical Research
1998-2003 Senior Research Scholar, Alberta Heritage Foundation for Medical
Research
1994 AHFMR Travel Award $ 1,800; Purpose: Presentation at the 10th
International Conference on AIDS, Yokohama, Japan, Aug. 7-11/94.
1994-1995 AHFMR Tech Commercialization Phase 1 Award: 'Retroviral vector for
gene therapy'; $15,000.
1996 AHFMR Lecturer Award: "HIV gene therapy and animal models" $700
1997 Howard Hughes Medical Institute, Scholar Nominee, Univ. of Alberta
1997-1999 Scholar, Lucille P. Markey Charitable Trust
2004-2005 Multiple Myeloma Research Foundation Senior Scholar
Patents (Issued and Pending)
Retroviral vectors Issued US Pat. No. 5,693,50.
Recombinant Hepatitis B Virus Vectors Issued US Pat. No. 5,981,274.
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Combination immunogene therapy Issued US Pat . No. 6,730,512.
Method of using mouse model for evaluation of HIV vaccines Issued US Pat. No.
6,248,721.
Animal model for evaluation of vaccines' Issued US and PCT filed (97)
Lentiviral vectors Issued US Pat. No. 6,207,455.
Lentiviral vectors Issued US Pat. No. 6,531,123.
Long term expression of lentiviral vector 60/385986, 2002.
Modulation of dendritic cell function PCT 7655 1WO, 2002.
Small interference RNA gene therapy PCT filed 2003.
Engineered dendritic cells for gene therapy US filed 2007.
Materials and methods for control of infections PCT/US/2007/011519 filed May 2007.
Production of mature and functional CD4 T cells developed from adult stem cells US
filed 2008.
Members and Services
1979-1980 Society of Phytopathology Studies
1983-1985 Foreign Language House, University of Iowa
1984-present American Society of Microbiology
1991-present American Association of Advancement of Science
1998-present American Society of Gene Therapy
1994-1997 Graduate Study Training Committee, Dept. of Medical Microbiology
and Immunology, University of Alberta
1995-1997 Alberta Gene Therapy Advisory Committee, Founder and Member
1996-1999 Ad hoc reviewer, Canadian Foundation for AIDS Research (CANFAR)
1996-1999 Ad hoc reviewer, NHRDP HIV/AIDS Biomedical/Clinical Review
Committee
1996-1999 Ad hoc reviewer, MRC Microbiology & Infectious Disease Committee
1997-1998 Ad hoc reviewer, BioTechniques
1997/8 Current Drugs Panel of Evaluators (Investigation Drug Data base, IDdb)
1997/8 NIH Special Emphasis Panel for HIV Vaccine Research.
1997-1998 NIH Special Review Committee, Pilot Studies on Gene Therapy Vectors
for Metabolic Diseases Exploratory Grant Applications, NIDDK/NIH.
1999 May NIDDK Special Grants Review Committee PPG Site Visit
1999-2001 USAMRMC, Breast Cancer Research Program, DOD, Genetics Panel
Member.
1997-2000 Council member, Faculty of Medicine, UF.
1997-1999 UF Senate, Faculty of Medicine
1998-2003 Interdepartment Graduate Study Program (IDP) Admission Committee
member, Genetics Concentration, College of Medicine, University of
Florida
2000-present Associate Editor, Current Gene Therapy, Bentham Science Publishers.
2001-2005 Ad hoc reviewer, Biotechnology Progress, Biotechnology &
Bioengineering
2002-present Ad hoc reviewer, Journal of Biomedical Science
2004-present Ad hoc reviewer, Human Gene Therapy
2001-present American Association for Cancer Research
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2002-present Member, European Society of Gene Therapy
2002 JDRF International, Program project write-in reviewer.
2003 January Member, NIAID Special Review Committee, AIDS Clinical &
Epidemiological Research Primate Core Immunology-Virology
Laboratories .
2003 Abstract reviewer for RNA virus vector category, and meeting moderator
in Advances in Technology and Applications, ASGT 2003 6th Annual
Meeting, Washington DC, June 4-8, 2003.
2002-present IAEA, Vienna, Austria, United Nation Consultant.
2004 March Member, NIAID Special Review Committee, HIV Vaccine Research and
Design Program (HIVRAD).
2004-2008 Member, NHLBI Special Emphasis Panel. The pathogenesis of HIV-
associated nephropathy.
2004 April Reviewer, Technology Development Corporation (TEDCO) Maryland
Technology Transfer Fund (MTTF).
Abstract reviewer, ASGT 2005 8th Annual Meeting, St. Louis, MO.
2005 June
International AIDS Society 4th Conference Abstract Reviewer, Sydney,
2007 July
Australia
2007-present Editorial Advisory Board: The Open Gene Therapy Journal, The Open
Vaccine Journal, The Open Genomics Journal, Bentham Open
2007-present Associate Editor, Journal of Formosa Medial Association, Elsevier
2008-prsent Member, American Society of Hematology
2008 Member, Study Section of National Research Program for Genomic
Medicine, National Science Council, Taiwan
Visiting Professorship
1993 Univ. of California, San Diego, CA.
Current Research Interests
My laboratory has been developing oncoretroviral and lentiviral vectors as gene transfer tools
and uses innovative approaches to study the development of stem cells and functional immune
cells. The emphasis of research has been to translate basic science into clinical treatment for
genetic diseases, infectious diseases and cancer. Research activities include characterization of
cellular and immune modulatory factors that are essential to the development of protective
immunity in patients. Innovative immunotherapy approaches are developed for the treatment of
infections and cancer. The long-term goal is to translate basic scientific discovery into clinical
applications.
Teaching Experience
Mechanism of Pathogenicity (MMID 415; 1994, 1996); DNA Cloning (MMID 516; 1994, 1995);
Bacterial Plasmids (MMID 520; 1994); Human Gene Therapy (Microbiology 514; 1995); Gene
Therapy (Oncology 520; 1995); Seminar in Immunology (IMM 502; 1996); Medical Virology
(Controversies in Medical Virology; 1998); IDP Section II on Molecular Biology (9/21, 9/24-
98); Immunology/Microbiology Journal Club Course (1998); Advanced IDP module (GMS6040)
Host-Pathogen Interactions (1998-present); IDP Human Genetics Module Gene Therapy
(12/4/98-/02); "Microbiology, Immunology and Immunotherapeutics" (PHA 5752, Spring 1999-
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2000); "Retrovirus and Hepatitis B Virus Replication", Advanced Virology (IDP, GMS6035,
1999-present); Gene Therapy: From Bench to Bedside (GMS 6059, 2003-present);
Fundamentals of Cancer Biology Cancer Vaccines (GMS 6065, 2003-present).
Invited International Research Workshops and Policy Conferences
OCDE Workshop Ottawa, Canada: '95 on Gene Delivery Systems'
Gene Therapy Policy Conference, NIH/FDA: Lentiviral Vectors for Gene Delivery 3-98.
International Atomic Energy Agency (IAEA), Division of Human Health, Consulting
Conference on Radiotherapy modifications of cancer treatments in AIDS patients, Vienna,
Austria, 2002-2006.
Invited Presentation/Workshops
Xth International AIDS Conference, Yokohama, Japan. Aug. 7-11, 1994. Topics: 'Tat-minus
HIV-1 Replication'.
University of Tokyo, The Third Department of Internal Medicine, Tokyo, Japan. Aug. 1994.
Topics: 'Human Gene Therapy and Retroviral Vectors'.
IBC's Fourth Conference on Gene Therapy. Washington, DC, USA. Nov. 14-15, 1994. Topics:
'Retroviral Vectors for Antiviral, Anti-Tumor and Gene Therapy'.
Division of Experimental Oncology, Dept. of Oncology, Cross Cancer Institute, Edmonton, Jan.
12, 1995. Topics: 'Tumor immunogene therapy: expression of T-cell costimulator B7-2 and
therapeutic cytokine GM-CSF gene using an improved retroviral vector'.
The Kennedy Krieger Research Institute, Dept. of Neurology, John's Hopkins Medical School,
Maryland, USA, April 1995. Topics: 'Anti-cancer Immunogene Therapy'.
Schepens Eye Research Institute, Harvard Medical School, Boston, USA, May 1995. Topics:
'Human Gene Therapy and Retroviral Vectors'.
The First Symposium on Gene Therapy in Canada, Toronto, June 26-27, 1995. Topics: 'Murine
Anti-HIV Models'.
University Hospitals Foundation, University of Alberta, Edmonton, Alberta, May 7, 1996.
"Health Talks: Cancer Immunogene Therapy: A Futuristic Treatment Strategy"
University of Calgary, AHFMR Lecture: "HIV gene therapy and animal models".
Western Pharmacology Society 40th Annual Meeting, Feb. 9-13, 1997. "Retroviral Vectors for
HIV and Cancer Gene Therapy".
National Institutes of Health, Advances in AIDS pathogenesis and Preclinical Vaccine
Development: Ninth Annual Meeting of the National Cooperative Vaccine Development
Groups for AIDS, May 4-7, 1997. "Development of a hu-PBL-SCID/beige mouse model for
the evaluation of HIV protective immunity".
University of Toronto Molecular Medicine Seminar Series, June 3, 1997. "B7-2 and GM-CSF
combination immunogene therapy in a human glioblastoma/humanized SCID mouse model".
National Institutes of Health, Office of AIDS Research, Nov. 16-18, 1997. "2nd HIV Primary
Infection Workshop".
University of Florida, Division of Dermatology & Cutaneous Surgery, March 12, 1998.
Immunogene therapeutic strategies against melanoma and other tumors .
UF/Shands Outreach Series Speaker, Leesburg Regional Medical Center, Sept. 18, 1998.
HIV protective immunity in multiply-exposed but uninfected homosexual and heterosexual
partners .
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RPR Gencell, Hayward, California, March 5, 1999. "An improved humanized mouse-human
tumor model for immunogene therapy".
Human Gene Therapy Research Institute Speaker, April. 5, 1999. "Efficacy and safety
improvement of lentiviral vectors for gene therapy applications"
University of Iowa, April. 6, 1999. "Efficacy and safety improvement of lentiviral vectors for
gene therapy applications".
University of Pennsylvania, June 4, 1999. Safety and efficacy improvements of lentiviral
vectors for gene therapy applications .
MLA 2001: An Information Odyssey, Orlando, Florida, May, 2001. Cancer Gene Therapy
2001 Hype or Hope? Medical Library Association s Molecular Biology and Genomics
Special Interest Group.
IBMS Academia Sinica, Taipei, Taiwan. July 5th, 2004,, National Cheng Kung University
Medical college, Tainan, Taiwan, July 8th, Chung Shan Medical University July 8th Lecture
Lentiviral modification of dendritic cell and T cell immunity .
Chang-Gong University, Taipei, Lecture Lentiviral immune modulation for cancer therapy -
current trend and future prospect", July 7, 2004.
CSMU 3rd AIDS Symposium, Taipei, Taiwan. July 10, 2004. Lectures: Lentiviral modification
of dendritic cell and T cell immunity and Lentiviral siRNA applications: HIV therapy and
functional genome discovery .
National Institute of Health, Thailand, Invited Lecturer Lentiviral siRNA for HIV/AIDS
therapy . July 14, 2004, Bangkok, Thailand.
National Veteran Administration Hospital, Taipei, Taiwan, July 12, 2005. Invited Lecturer
Lentiviral modulation of dendritic cell function for cancer therapy .
National Institute of Aging, NIH, Bethesda, Maryland, Invited Lecturer Immunotherapy for
cancer and HIV/AIDS . March 2007.
Shanghai Daopei Hospital, Shanghai, China, March 2008. Immunotherapy for infectious
diseases and cancer current and future prospective
The 19th CASPAF Annual Conference, May 24, 2008, Orlando FL. From genes to cells to
novel immunotherapies for cancer .
Bibliography
Ficht, T.A., Chang, L.-J. and Stoltzfus, C.M. (1984). Avian sarcoma virus gag and env genes
contain a common amino-terminal sequence. Proc. Natl. Acad. Sci. USA 81, 362-366.
Chang, L.-J. and Stoltzfus, C.M. (1985). Cloning and nucleotide sequences of cDNA spanning
the splice junctions of Rous sarcoma virus mRNAs. J. Virol. 53, 969-972.
Chang, L.-J. and Stoltzfus, C.M. (1985). Gene expression from both intronless and intron-
containing Rous sarcoma virus clones is specifically inhibited by anti-sense RNA. Mol.
Cell. Biol. 5, 2341-2348.
Perlman, S., Bolger, E., Chang, L.-J. and Stoltzfus, C.M. (1986). Synthesis of genomic and
subgenomic RNA in MHV-A59 cells have different requirements for protein synthesis.
Virus Res. 6, 261-272.
Chang, L.-J. and Stoltzfus, C.M. (1987). Inhibition of Rous sarcoma virus replication by
antisense RNA. J. Virol. 61, 921-924.
Stoltzfus, C.M., Chang, L.-J., Cripe, T.P. and Turek, L.P. (1987). Efficient transformation by
Prague A Rous sarcoma virus plasmid DNA requires the presence of cis-acting regions
within the gag gene. J. Virol. 61, 3401-3409.
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Chang, L.-J., Pryciak, P., Ganem, D. and Varmus, H.E. (1989). Biosynthesis of the reverse
transcriptase of hepatitis B viruses involves de novo translational initiation not ribosomal
frameshifting. Nature 337, 364-368.
Chang, L.-J., Dienstag, J., Ganem, D. and Varmus, H.E. (1989). Detection of antibodies
against hepatitis B virus polymerase antigen in hepatitis B virus-infected patients.
Hepatology 10, 332-335.
Hirsch, R., Lavine, J., Chang, L.-J., Varmus, H. E. and Ganem, D. (1990). Polymerase gene
products of hepatitis B virus are required for genomic RNA packaging as well as for
reverse transcription. Nature 344, 552-555.
Chang, L.-J., Ganem, D. and Varmus, H. E. (1990). Mechanism of translation of the
hepadnaviral polymerase (P) gene. Proc. Natl. Acad. Sci. USA 87, 5158-5162.
Chang, L.-J., Hirsch, R., Ganem, D. and Varmus, H. E. (1990). Effects of insertional and point
mutations on the functions of the duck hepatitis B virus polymerase. J. Virol. 64, 5553-
5558.
Chang, L.-J., McNulty, E. and Martin, M. (1993). Human immunodeficiency viruses
containing heterologous enhancer/promoters are replication competent and exhibit different
lymphocyte tropisms. J. Virol. 67, 743-752.
Dimitrov. D., Willey, R., Sato, H., Chang, L.-J., Blumenthal, R. and Martin, M. (1993).
Quantitation of HIV-1 infection kinetics. J. Virol. 67, 2182-2190.
Amendt. B. A., Hesslein, D., Chang, L.-J. and Stoltzfus, C. M. (1994). Presence of negative
and positive cis-acting RNA splicing elements within and flanking the first tat coding exon
of the human immunodeficiency virus type 1. Mol. Cell. Biol.14, 3960-3970.
Lawrence, R., Chang, L.-J., Siebenlist, U., Bressler, P. and Sonenshein, G. E. (1994). Vascular
smooth muscle cells express a constitutive NF-kB-like activity. J. Biol. Chem. 269,
Chang, L.-J., Chen, Y.-M. A., Chen, M.-Y., Chou, C.-C., Twu, S.-J. and Huang, L.-M. (1995).
Meeting report: Xth International AIDS Meeting, Yokohama, Japan. HIV 10 years later:
where do we stand now? J. Biomedical Sci. 2, 1-11.
Robinson, D., Elliott, J. F. and Chang, L.-J. (1995). Retroviral vector with a CMV-IE/HIV-
TAR hybrid LTR gives high basal expression levels and is upregulated by HIV-1 Tat.
Gene Therapy 2, 269-278.
Chang, L.-J. and Zhang, C. (1995). Infection and replication of Tat- human immunodeficiency
viruses: genetic analyses of LTR and tat mutants in primary and long-term human
lymphoid cells. Virology 211, 157-169.
Kao, G. Y., Chang, L.-J. and Allen, T. M. (1996). Use of targeted cationic liposomes in
enhanced DNA delivery to cancer cells. Cancer Gene Therapy 3, 250-256.
Dickie, P., Gazzinelli, R., and Chang, L.-J. (1996). Models of HIV type 1 proviral gene
expression in wild-type HIV and MLV/HIV transgenic mice. AIDS Research and
Human Retroviruses 12, 1103-1116.
Dickie, P., Mounts, P., Miller, G., Frederickson, T., Chang, L.-J. and Martin, M. A. (1996).
Myopathy and spontaneous Pasteurella pneumotropica infection in a transgenic mouse
model of HIV-infected disease. J. of AIDS and Human Retrovirology 13, 101-116.
Zhang, C., Cui, Y., Houston, S. and Chang, L.-J. (1996). Protective immunity to HIV-1 in
SCID/beige mice reconstituted with peripheral blood lymphocytes of exposed but
uninfected individuals. Proc. Natl. Acad. Sci. USA 93, 147**-*****.
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Cui, Y. and Chang, L.-J. (1997). Computer-assisted, quantitative cytokine ELISPOT analysis
of human immune effector cell function. BioTechniques 22, 1146-1149.
Zhang, C, Colin, R. and Chang, L.-J. (1997) Cell cycle inhibitory effects of HIV and SIV Vpr
and Vpx in the yeast Schizosaccharomyces pombe. Virology 230, 103-112.
Parney, I. F., Petruk, K. C., Zhang, C., Farr-Jones, M. Sykes, D. and Chang, L.-J. (1997) GM-
CSF and B7-2 combination immunogene therapy in an allogeneic hu-PBL-SCID mouse-
human glioblastoma multiforme model. Human Gene Therapy 8, 1073-1085.
Kilani, R. T., Chang, L.-J., Garcia-Lloret, M., Hemmings, D., Winkler-Lowen, B. and Guilbert,
L. J. (1997) Placental trophoblasts resist infection by multiple HIV-1 variants even with
CMV co-infection but support HIV replication after provirus transfection. J. Virol. 71,
6359-6372.
Chaisomchit, S., Tyrrell, D. L. J. and Chang, L.-J. (1997). Development of replicative and
non-replicative human hepatitis B virus vectors. Gene Therapy 4, 1330-1340.
Cui, Y. and Chang, L.-J. (1998) A rapid and reliable bioassay for functional human interleukin-
12 by interferon-g ELISPOT. Prep. Biochem. and Biotechnol. 28 (1), 23-36.
Ghahary, A., Tredget, E. E., Chang, L.-J., Scott, P. G. and Shen, Q. (1998) Genetically
modified dermal keratinocytes express high levels of transforming growth factor-b1. The J.
of Investigative Dermatology 110, 800-805.
Chang, L.-J., V. Urlacher, T. Iwakuma, Y. Cui, and J. Zucali (1999). Efficacy and safety
analyses of a recombinant human immunodeficiency virus derived vector system. Gene
Therapy 6, 715-728.
Cui, Y., T. Iwakuma and L.-J. Chang (1999). Contributions of viral splice sites and cis-
regulatory elements to lentivirus vector functions. J. Virol. 73, 6171-6176.
Iwakuma T., Y. Cui, and L.-J. Chang (1999). Self-inactivating lentiviral vectors with U3 and
U5 modifications. Virology 261, 120-132.
Parney, I., M. A. Farr-Jones, L.-J. Chang and K. C. Petruk (2000). Human glioma
immunobiology in vitro: implications for immunogene therapy. Neurosurgery 46. 1169-
1177; discussion 1177-1178.
Chang, L.-J., C. Chen, V. Urlacher and Tse-fun Lee (2000). Differential apoptotic effects of
primate lentivial Vpr and Vpx in mammalian cells. Journal of Biomedical Science 7(4):
277-338.
Higashikawa, F. and Chang, L.-J. (2001). Kinetic analyses of simple and complex retroviral
vectors. Virology. 280: 124-131.
Parney I. F., Farr-Jones M. A., Koshal A., Chang L.-.J, Petruk K. C. .(2002) Human brain
tumor cell culture characterization after immunostimulatory gene transfer. Neurosurgery.
50(5): 1094-1102.
Zaiss, A.-K., S. Son and Chang, L.-J. (2002) RNA 3 readthrough of oncoretrovirus and
lentivirus: implications for vector safety and efficacy. J. Virol. 76, 7209-7219.
Parney, I. F., Farr-Jones, M. A., Kane, K., Chang, L.-J., and Petruk, K. C. (2002) Human
autologous in vitro models of glioma immunogene therapy using B7-2, GM-CSF, and IL-
12. Canadian Journal of Neurological Sciences 29: 267-275.
Chang, L.-J. (2003) Vector integration: pest not guest. Gene Therapy 10: 193-194 (News and
Commentary).
He, J. and Chang, L.-J. (2004) Functional characterization of hepatoma-specific stem cell
antigen-2. Molecular Carcinogenesis 40, 90-103.
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Chen, X., He, J. and Chang, L.-J. (2004) Alteration of T cell immunity by lentiviral
transduction of dendritic cells. Retrovirology 1, 37.
Chen, X. and Chang, L.-J. (2005) Mycoplasma-mediated alterations of in vitro generation and
functions of human dendritic cells. J. Biomed. Sci. 12, 31-46.
Chang, L.-J., Liu, X. and He. J (2005) Lentiviral siRNAs targeting multiple highly conserved
RNA sequences of human immunodeficiency virus type 1. Gene Therapy 12, 1133-44.
Oka, M., Meacham, A. M., Hamazaki, T., Rodic, N., Chang, L.-J. and Terada, N. (2005) De
novo DNA methyltransferase Dnmt3a/3b primarily mediates the cytotoxic effect of 5-aza-
2'-deoxycytidine. Oncogene 24, 3091-3099.
Lin, G, Hayashi, N., Carrion, R., Chang, L.-J., Lue, T.F., and Lin C.-S. (2005) Improving
erectile function by silencing phosphodiesterase-5. J. Urol. 174, 1142-48.
He, J, Yang, Q and Chang, L.-J. (2005) DNA methylation and chromatin modifications Are
coupled with lentiviral transgene silencing in murine embryonic carcinoma cells. J. Virol.
79, 13497-508.
Shao, P.-L., Hsueh, P.-R., Chang, L.-Y., Lu, C.-Y., Kao, C.-L., Chiang, Y.-P., Huang, H.-Y.,
Huang, F.-Y., Lee, C.-Y., Chang, L.-J., Wu, T.-C. and Huang, L.-M. (2005) Development
of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe
acute respiratory syndrome. J. Biomed. Sci. 12, 59-64.
Xiong, C, Tang, D-Q, Xie, C-Q, Zhang, L., Xu, K-F, Thompson, WE, Chou, W., Gibbons, GH,
Chang, L-J, Yang, L-J, Chen, YE (2005) Genetic engineering of human embryonic stem
cells with lentiviral vectors. Stem Cells and Development 14, 367-377.
Tang, D.-Q, Lu, S., Sun, Y.-P., Rodrigues, E., Chou, W., Yang, C., Cao, L.-Z., Chang, L.-J.
and Yang, L.-J. (2006) Reprogramming liver-stem WB cells into functional insulin-
producing cells by persistent expression of Pdx1- and Pdx1-VP16 mediated by lentiviral
vectors. Lab. Invest. 86, 83-93.
Parney, I. F., Chang, L.-J., Farr-Jones, M. A., Hao, C., Smylie, M., Petruk, K. C. (2006)
Technical hurdles in a pilot clinical trial of combined B7-2 and GM-CSF immunogene
therapy for glioblastomas and melanomas. J. Neuro-Onco. 78, 71-80.
Oka, M., Rodic, N., Graddy, J., Chang, L.-J. and Terada, N. (2006) CpG sites preferentially
methylated by Dnmt3a in vivo. J. Biol. Chem. 28, 9901-9908.
Wang, B, He, J., Chen, L. and Chang, L.-J. (2006) An effective cancer vaccine modality:
lentiviral modification of dendritic cells expressing multiple cancer-specific antigens.
Vaccine 24, 3477-89.
Tang, D.-Q, Cao, L.-Z., Chou, W., Shun, L., Farag, C., Atkinson, M. A., Li, S.-W., Chang, L.-
J. and Yang, L.-J. (2006) Role of Pax4 in Pdx1-VP16-mediated liver-to-endocrine pancreas
transdifferentiation. Lab. Invest. 86, 829-41.
Chen, X., Wang, B. and Chang, L.-J. (2006) Induction of primary anti-HIV CD4 and CD8 T
cell responses by dendritic cells transduced with self-inactivating lentiviral vectors.
Cellular Immunology 243, 10-18.
Walton, N. M., Sutter, B. M., Chen, H.-X., Chang, L.-J., Roper, S. N., Scheffler, B. and
Steindler, D. A. (2006) Derivation and large-scale expansion of multipotent astroglial
neural progenitors from adult human brain. Development 133, 3671-3681.
Ryu, B.-Y., Orwig, KE, Oatley, JM, Lin, CC, Chang, L.-J., Avarbock, MR and Brinster, RL
(2007) Efficient generation of transgenic rats through the male germline using lentivrial
transduction and transplantation of spermatogonial stem cells. J. Andrology 28, 353-360.
Yang, Q, Lucas, A., Son, S. and Chang, L.-J. (2007) Overlapping enhancer/promoter and
transcriptional termination signals in the lentiviral long terminal repeat. Retrovirology 4-4.
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Cheng, H., Wolfe, SH, Valencia, V., Qian, K., Shen, L., Phillips, MI, Chang, L.-J. and Zhang,
YC. (2007) Efficient and persistent transduction of exocrine and endocrine pancreas by
adeno-associated virus type 8. J. Biomed. Sci. 14, 585-594.
Han, S., Wang, B., Yang, L.-J., Zucali, JR, Moreb, JS, and Chang, L.-J. (2008) Overcoming
immune tolerance against multiple myeloma with lentiviral calnexin-engineered dendritic
cells. Molecular Therapy 16, 269-279.
Moreb JS, Baker HV, Chang L-J, Amaya M, Lopez MC, Ostmark B, Chou W. (2008) ALDH
isozymes downregulation affects cell growth, cell motility and gene expression in lung
cancer cells Molecular Cancer 7, 87.
Wang, B., Han, S., Lien, L., Guo, L. and Chang, L.-J. (2009) Lentiviral calnexin-modified
dendritic cells promote expansion of high-avidity effector T cells with central memory
phenotype. Immunology (in press).
Patel, E., Wang, B., Lien, L., Wang, Y., Yang, L.J., Moreb, J.S., and Chang, L.-J. (2009)
Diverse T cell differentiation potentials of human fetal thymus, fetal liver, cord blood and
adult bone marrow CD34 cells on lentiviral Delta-like 1-modified mouse stromal cells.
Immunology (in press).
Lin, G., Wang, G., Liu, G., Yang, L.-J., Chang, L.-J., Lue, T. F. and Lin, C.-S. (2009)
Treatment of Type 1 Diabetes with Adipose Tissue-Derived Stem Cells Expressing
Pancreatic Duodenal Homeobox 1. Stem Cells and Development (in press)
Books, Contributor of Chapter
Chang, L.-J. and Zaiss, A.-K. (2001) Methods for the preparation and use of lentivirus vectors.
Methods in Molecular Medicine, Gene Therapy Protocols, 2nd Ed., pp 303-318, Ed. Jeffrey
Morgan, Humana Press, Inc..
Chang, L.-J. and Zaiss, A.-K.(2002) Self-inactivating lentiviral vectors in combination with a
sensitive Cre-loxP reporter system. Methods in Molecular Medicine. Viral Vectors for
Gene Therapy: Methods and Protocols, pp 367-382, Ed. John Walker, Humana Press, Inc..
Kawasome, H., Hamazaki, T., Minamino, T. and Chang, L.-J. (2002) Signal transduction study
using gene-targeting embryonic stem cells. Methods and Protocols. Cancer Cell Signaling,
pps. 35-46. Series: Methods in Molecular Biology, Humana Press, Inc..
Cui, Y. and Chang, L.-J. (2003) Detection and selection of lentiviral vector transduced cells.
Methods in Molecular Biology Vol. 229: Lentivirus Gene Engineering Protocols pp 69-
85, Ed. Maurizio Federico, Humana Press, Inc..
Oka, M. Chang, L.-J., Costantini, F., and Terada, N. (2005) Lentivirus mediated gene transfer
in embryonic stem cells. Series: Methods in Molecular Biology Embryonic Stem Cells 2.
Costantini, F., Oka, M., Terada, N. and Chang, L.-J. (2006) Embryonic Stem Cell Protocols.
Volume I, Isolation and Characterization, Second Edition pp. 273-282. Series: Methods in
Molecular Biology, Humana Press Inc..
Chang, L.-J. (2008) Immune response of lentiviral vectors. In Immunology of Gene
Therapy, John Wiley and Sons, Inc.. (in press)
Han, S. and Chang, L.-J. (2008) Immunity of lentiviral vector-modified dendritic cells. In
Cancer Gene Therapy, Second Edition, Series: Methods in Molecular Medicine,
Humana Press Inc.. (in press)
Chang, L.-J. (2009) Lentiviral vector transduction of dendritic cells for novel vaccine
strategies. In Lentiviral Gene Engineering Protocols, Second Edition, Series: Methods in
Molecular Biology, Humana Press Inc. (in press)
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Reviews
Chang, L.-J., Zhang, C., Robinson, D. and Dickie, P. (1996). Murine models for the evaluation
of anti-HIV strategies. Transfusion Science 17, 89-98.
Chang, L.-J. and Gay, E. (2001) The molecular genetics of lentiviral vectors - current and
future perspectives. Current Gene Therapy 1, 237-251.
Chang, L-J. and He, J. (2001) Retroviral vectors for gene therapy of AIDS and cancer .
Current Opinion in Molecular Therapeutics 3, 468-475.
Parney, I. F. and Chang, L.-J. (2002) Cancer immunogene therapy. Journal of Biomedical
Sciences 10, 37-43.
Chang, L.-J., Chang, L.-Y. and Huang, L.-M. (2008) Besides increasing surveillance and
waiting for an effective vaccine to emerge in the future, what else can be done to save the
lives of HFMD victims? J Formos Med Assoc 107, 589-590.
Abstracts
Ficht, T. A., Chang, L.-J. and Stoltzfus, C. M. Amino terminal analysis of the primary
translation products of the gag and env genes of avian RNA tumor viruses. 1983 RNA Tumor
Virus Meeting, Cold Spring Harbor.
Stoltzfus, C. M., Lorenzen, S. and Chang, L.-J. Comparison of src mRNA splicing in cells
infected with different strains and intron deletion mutants of Rous Sarcoma Virus. 1984 RNA
Tumor Virus Meeting, Cold Spring Harbor.
Chang, L.-J., Ficht, T. A. and Stoltzfus, C. M. Accurate mapping of the splice junction sites of
Rous sarcoma virus mRNAs. 1984 American Society of Microbiology, North Central
Branch Annual Meeting, Milwaukee, Wisconsin.
Chang, L.-J., Cooling, L., Turek, L. and Stoltzfus, C. Expression of intronless RSV DNA
constructs and specific inhibition by anti-sense RNA. 1985 RNA Tumor Virus Meeting,
Cold Spring Harbor.
Chang, L.-J. and Stoltzfus, C. M. Mutagenesis of 5'-leader of Rous sarcoma virus: effects on
expression of env and src gene mRNAs. 1986 RNA Tumor Virus Meeting, Cold Spring
Harbor.
Chang, L.-J., Ganem, D. and Varmus, H. E. Study of the expression strategy of hepadnavirus
pol genes. 1987 Hepatitis B Virus Meeting, Cold Spring Harbor.
Chang, L.-J., Ganem, D. and Varmus, H. E. Genetic analysis of the expression strategy of the
hepadnaviral pol gene. 1987 Hepatitis B Virus Meeting, University of California, San Diego.
Chang, L.-J., Hirsch, R., Colgrove, R., Ganem, D. and Varmus, H. E. Analysis of the DHBV
pol gene by site-specific mutagenesis. 1989 Hepatitis B Virus Meeting, Cold Spring Harbor.
Chang, L.-J., Ganem, D. and Varmus, H. E. Studies on the translational mechanism of the
DHBV polymerase gene. 1989 Hepatitis B Virus Meeting, Cold Spring Harbor.
Hirsch, R., Lavine, J., Chang, L.-J., Varmus, H. E. and Ganem, D. Pol translation is required
for the encapsidation of DHBV genomic RNA. 1989 Hepatitis B Virus Meeting, Cold
Spring Harbor.
Chang, L.-J, McNulty, E. and Martin, M.A. Human immunodeficiency viruses with
heterologous enhancers exhibit different infectivity in human T cells. 1991 RNA Tumor
Virus Meeting, Cold Spring Harbor.
Chang, L.-J. and Martin M. A. Tat-minus HIV mutants containing CMV enhancer elements
can infect human T-cell lines. 1992 RNA Tumor Virus Meeting, Cold Spring Harbor.
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Chang,, L.-J. and Martin M. A. tat-independent HIV-1 replication and generation of chronic
high producer. 1993 RNA Tumor Virus Meeting, Cold Spring Harbor.
Robinson, D and Chang, L.-J. Design of an improved retroviral vector for anti-HIV gene
therapy. 1994 UA-UC Conference on Infectious Diseases. Alberta, Canada.
Chang, L.-J. Tat-minus HIV-1 replication: implication on alternative Tat function. 1994
Canadian conference on AIDS research, Toronto, Canada.
Chang, L.-J. Tat-minus HIV-1 replication. 1994 The 10th International Conference on AIDS,
Yokohama, Japan.
Robinson, D. and Chang, L.-J. Gene therapy vectors derived from HIV and MLV for anti-HIV
gene delivery. 1995 The 5th annual conference on HIV/AIDS research, Winnipeg,
Manitoba. Can. J. Infect. Dis. 6 Suppl. B, 11B.
Chang, L.-J., Zhang, C. and Sherburne, R. Persistent productive infection of human
immunodeficiency virus type 1: effects of LTR and tat mutations and viral attenuation. 1995
Retroviruses Meeting, Cold Spring Harbor.
Robinson, D. and Chang, L.-J. An improved retroviral anti-HIV vector exhibits efficient
protection of human lymphocytes from HIV infection. 1995 First Conference on Stem Cell
Gene Therapy: Biology and Technology, Rockville, Maryland.
Chang, L.-J. Retroviral vectors for HIV and Cancer Gene Therapy. 1995 Alberta Gene
Therapy Meeting, Red Deer, Alberta.
Allen, T. M., Chang, L.-J. and Kao, G. Y. Increased delivery of DNA to cancer cells by ligand
targeted cationic liposomes. 1995. Alberta Gene Therapy Meeting, Red Deer, Alberta.
Petruk, K. C., Parney, I. F., Chang, L.-J. and M. Farr-Jones. Immuno gene therapy for
Glioblastoma. 1995. Alberta Gene Therapy Meeting, Red Deer, Alberta.
Zhang, C., Houston, S. and Chang, L.-J. Chang. Resistance to human immunodeficiency virus
infection of SCID/beige mice reconstituted with peripheral blood mononuclear cells from
high-risk HIV-seronegative individuals. 1996. (We.A.3001) XI International Conference on
AIDS, Vancouver.
Robinson, D. and Chang, L.-J.. Improved anti-HIV efficacy for retroviral gene therapy vectors
carrying a Tat-responsive promoter and a conserved HIV packaging signal. 1996.
(We.A.274) XI International Conference on AIDS, Vancouver.
Ruhangiz, K., Winkler, B., Chang, L.-J. and Guilbert, L. J.. Placental trophoblasts resist
infection by HIV-1 but support replication and release of virus after provirus transfection.
1996. (We.B.194) XI International Conference on AIDS, Vancouver.
Robinson, D. and Chang, L.-J.. A retroviral vector for HIV-1 and HIV-2 gene therapy based
on a chimeric Tat-responsive element and a conserved HIV-1 packaging signal. 1996. Gene
Therapy Meeting, Cold Spring Harbor.
Cui, Y. and Chang, L.-J.. Immunogene therapy on treatment of breast cancer using humanized
SCID/beige mouse model. Nov. 7-8 1996. Alberta Cancer Board Annual Research Meeting,
Calgary, Alberta.
Parney, I. F., Farr-Jones, M. A., Chang, L.-J. and Petruk, K. C. In vitro immunobiology of
human blioblastoma multiforme: implications for gene therapy. Nov. 7-8 1996. Alberta
Cancer Board Annual Research Meeting, Calgary, Alberta.
Parney, I. F., Petruk, K. C., Zhang, C., Farr-Jones, M. A. and Chang, L.-J.. Immunogene
therapy for glioblastoma multiforme using B7-2 and GM-CSF in a allogeneic human
tumor/human lymphocyte xenograft model. Nov. 7-8 1996. Alberta Cancer Board Annual
Research Meeting, Calgary, Alberta.
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Morin, K. W., Chang, L.-J., Knaus, E. E. and Wiebe, L. I.. Metabolic entrapment as a
mechanism for detecting in vitro and in vivo gene transfer and expression. Nov. 7-8 1996.
Alberta Cancer Board Annual Research Meeting, Calgary, Alberta.
Chang, L.-J.. Retroviral vectors for HIV and cancer gene therapy. Feb. 9-13, 1997. Western
Pharmacology Society 40th Annual Meeting, Banff, Alberta, Canada.
Parney, I. F., Farr-Jones, M. A., Chang, L.-J. and Petruk, K. C. In vitro immunobiology of
human glioblastoma multiforme: implications for gene therapy. April 1997. American
Association of Neurologic Surgeons.
Parney, I. F., Petruk, K. C., Zhang, C., Farr-Jones, M. A. and Chang, L.-J. Immunogene
therapy for glioblastoma multiforme using B7-2 and GM-CSF in an allogeneic human
tumor/human lymphocyte xenograft model. April 1997. American Association of
Neurologic Surgeons.
Chang, L.-J., Parney, I. F. and Petruk, K. C. GM-CSF and B7-2 combination immunogene
therapy in an allogeneic hu-PBL-SCID mouse / human glioblastoma multiforme model.
April 13-19, 1997. Molecular and cellular biology of gene therapy. Keystone Symposia,
Snowbird Utah.
Zhang, C., Rasmussen, C. and Chang, L.-J. Cell cycle inhibitory effects of HIV and SIV vpr
and vpx in the yeast Schizosaccharomyces pombe. April 8-13, 1997. AIDS Pathogenesis.
Keystone Symposia, Keystone.
Cui, Y., Zhang, C. and Chang, L.-J. An improved humanized mouse-human tumor model for
immunogene therapy. July 7-10, 1997. First International Symposium on Gene Therapy --
Beijing.
Chaisomchit, S., Chang, L.-J. and Tyrrell, D. L. J. Development of hepatitis B virus vector:
demonstrating a replicative and nonreplicative type of vectors. Sept. 21-25, 1997. Institut
Pasteur-Paris.
Chang, L.-J. and Cui, Y. Efficacy and safety of HIV-1 based lentiviral vectors. 1st Annual
Meeting, American Society of Gene Therapy. May 28-31, 1998. Seattle, Washington.
Iwakuma T., Cui, Y. and Chang, L.-J. An improved lentiviral vector system: modifications of
5 and 3 LTR s. Gene Therapy Meeting, Sept. 23-27, 1998. Cold Spring Harbor, New York.
Cui, Y., Iwakuma, T. and Chang, L.-J. Negative and positive regulatory effects of splice sites,
gag, env and RRE on lentiviral vector functions. Gene Therapy Meeting, Sept. 23-27, 1998.
Cold Spring Harbor, New York.
Zucali, J. R., Zaiss, A.-K. and Chang, L.-J. Lentiviral transduction of human primary
hematopoietic stem cells and hematopoietic cell lines. 2nd Annual Meeting, American
Society of Gene Therapy. June 9-13, 1999 Washington, DC.
Cui, Y. and Chang, L.-J. Mutational analyses of 5' splice site and the packaging signal in
lentiviral transducing vectors. 2nd Annual Meeting, American Society of Gene Therapy.
June 9-13, 1999 Washington, DC.
Gay, E. and Chang, L.-J. A lenti-retro hybrid vector system: packaging of retroviral vector
genomes into lentiviral particles. 2nd Annual Meeting, American Society of Gene Therapy.
June 9-13, 1999 Washington, DC.
Dennis Kunimoto, Fei Zhong, Lung-ji Chang. Human PBL SCID mice as a model for human
immunity to TB. IUATLD, Vancouver, BC, February 24-26, 2000
Zaiss, A.-K.and Chang, L.-J. Differential regulation of retro- and lentiviral transgene
expression. 3rd Annual Meeting, American Society of Gene Therapy. May 31-June 4, 2000
Denver, Colorado.
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Chang, L.-J. and Zaiss, A.-K. Pseudotyping lentiviral vectors with Sendai and Ebola virus
envelope glycoproteins. 3rd Annual Meeting, American Society of Gene Therapy. May 31-
June 4, 2000 Denver, Colorado.
Zaiss, A.-K. and Chang, L.-J. Analyses of the mechanism of differential transgene expression
in retro- and lentiviral vectors. Cold Spring Harbor Laboratory Gene Therapy Meeting, Sept.
25-29, 2000. Cold Spring Harbor, New York.
Gay, E. and Chang, L.-J. Analyses of minimal lentiviral vector elements using a chimeric
MLV/HIV vector approach. Cold Spring Harbor Laboratory Gene Therapy Meeting, Sept.
25-29, 2000. Cold Spring Harbor, New York.
Chang, L.-J., Higashikawa, F. and He, J. Expression array analyses of primary human
endothelial cells transduced with adenoviral, retro- and lentiviral vectors. ASGT Annual
Meeting. May 28-31, 2001. Seattle, Washington.
Gay, E. and Chang, L.-J. A hybrid HIV/MLV transducing vector. ASGT 4th Annual
Meeting.May 28-31, 2001. Seattle, Washington.
Son, S., Zaiss, A.-K. and Chang, L.-J. The safety and efficacy improvement of retroviral
vectors: a lesson from lentiviral vectors. ASGT 4th Annual Meeting.May 28-31, 2001.
Seattle, Washington.
Lucas, A. and Chang, L.-J. The chicken beta-globin HS4 insulator protects the long term
expression of lentiviral vectors. ASGT 5th Annual Meeting. June 5-9, 2002. Boston, MA.
He, J. and Chang, L.-J. Identification of highly differentially expressed tumor antigens by a
suppression subtractive hybridization method and functional characterization using small
interference RNA . ESGT 10th Annual Meeting. Oct. 13-16, 2002. Antibes Juan Les Pins,
France.
Chen, X. and Chang, L.-J. Bimodual modification of human monocyte-derived dendritic cells
by HEK 293-T cell conditioned medium: Implications in cancer gene therapy modality.
ESGT 10th Annual Meeting. Oct. 13-16, 2002. Antibes Juan Les Pins, France.
Chen, X, He, J. and Chang, L.-J. Impaired Th1 response induced by lentiviral vector-
transduced dendritic cells. The Southeastern Branch of the American Society for
Microbiology. Nov. 7-9, 2002. Gainesville, Florida.
Yang, Q., He. J. and Chang, L.-J. An inducible lentiviral vector system. The Southeastern
Branch of the American Society for Microbiology. Nov. 7-9, 2002. Gainesville, Florida.
Chang, LJ, He, J. and Chen X. Development of dendritic cell vaccines using lentiviral vectors
and immune modulators. 11th International Conference on Gene Therapy of Cancer. Dec.
12-14, 2002, San Diego, CA.
He, J. and Chang, L.-J. Long Term Silencing of Tumor Specific Gene by Lentiviral Vectors
Expressing Small Interference RNA. ASGT 6th Annual Meeting. June 4-8, 2003. Washington
DC.
Chang, L.-J., Chen, X., He, J. and Liu X. Impaired Th1 response induced by HIV-1 vector
infected dendritic cells. AIDS Vaccine 2003 Conference. Sept. 18-21, 2003. New York.
Chang, L.-J. and He. J. High efficiency functional disease genome discovery using an
advanced lentiviral vector system and small interfering RNA technology. 1st IBC Asia-
Pacific Biotechnology Conference, Drug Discovery Technology. June 3-6, 2003. Singapore.
Chen, X., He. J. and Chang, L.-J. Improved Dendritic Cell Vaccine Modality Using Lentiviral
Vectors. 95th AACR Annual Meeting, Orlando FL. March 27-21, 2004.
Xi, B., Huang, H., Alfieri, A., Sharma, A., Mohan, S., Lee, S.W., Chang, L.-J., Guha, C.. A
Lentiviral Vector Expressing Small Interfering RNAs (siRNA) Targeting the ATM gene
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enhances radiosensitivity of human tumor cell lines. 95th AACR Annual Meeting, Orlando
FL. March 27-21, 2004.
Tang, D, Cao, L, Farag, C., Chang, L.-J., and Yang, L. The Critical Role of Pax4 in Pdx1
mediated liver to endocrine pancreas transdifferentiation. America Diabetes Associateion
Annual Meeting, Orlando, June 2004.
Chang, L.-J and He, J. Lentiviral siRNAs targeting multiple highly conserved sequences of
human immunodeficiency virus type 1. XV International AIDS Conference, Bangkok,
Thailand, July 11-16, 2004.
He, J., Yang, Q. and Chang, L.-J. Characterization of molecular mechanisms of lentiviral
transgene silencing in murine embryonic carcinoma cells. XII Annual Congress of European
Society of Gene Therapy, Tampere, Finland, November 4-7, 2004.
Wang, B., He, J. and Chang, L-J. Identification of highly differentially expressed cancer-
specific genes and induction of anti-cancer immunity by lentiviral vector-transduced
dendritic cells in a Balb/c murine hepatoma model. XII Annual Congress of European
Society of Gene Therapy, Tampere, Finland, November 4-7, 2004.
Yang, Q., He, J. and Chang, L.-J. The U3 of lentiviral LTR contains genetic elements with
overlapping functions of enhancer/promoter and transcriptional termination. XII Annual
Congress of European Society of Gene Therapy, Tampere, Finland, November 4-7, 2004.
Tang, D.-Q., Chen, X., Chang, L.-J., Yang, L.-J. and Chen, Y.-Q. High-efficiency transduction
of human embryonic stem cells by lentiviral vectors. Keystone Symposium, 2004.
Wang, B., He, J. and Chang, L.-J. Effective cancer vaccine strategy by lentiviral transduction
of highly differentially-expressed cancer genes into dendritic cells in a Balb/c murine
hepatoma model. Keystone Synposium 2005.
Yang, Q, Chou, W, and Chang, L.-J. Dissecting overlapping functions of transcriptional
enhancer and polyadenylation in the lentiviral U3. ASGT 8th Annual Meeting. June 1-5,
2005. St. Louis, MO.
Wang, B., He, J. and Chang, L.-J. Effective cancer immunotherapy using lentiviral delivery of
multiple highly differentially-expressed cancer genes into dendritic cells in a murine
hepatoma model. ESGT 13th Annual Meeting. Oct. 29-Nov. 1, 2005, Prague.
Chang, LJ, Han, S., Kuo, L., Lien, L., Tang, J., Tong, CR, Wu, T. and Lu, DP. Correlation of
GvHD, GvL and anti-virus immunity in an acute myeloid leukemia patient through in vivo
tracking of therapeutic T cells. Keystone Symposium The Potent New Anti-tumor
Immunotherapies- March 28-April 2, 2007, Banff, Alberta.
Wang, B, Han, S., Moreb, J. and Chang, LJ. Potent expansion o fhigh-avidity cytotoxic T
lymphocytes with central memory phenotype by lentiviral modification of dendritic cells
supraphysiologically expressing calnexin. Keystone Symposium The Potent New Anti-
tumor Immunotherapies- March 28-April 2, 2007, Banff, Alberta.
Chang, LJ, Kuo, L., Lien, L., Wang, Y., Han, S., Tong, CR, Lu, DP, and Wu, T. An
accelerated T cell therapy rescues hematopoietic cell transplant patients from life-threatening
post-transplant lymphoproliferative disease and cytomegalovirus interstitial pneumonia.
AACR Annual Meeting, April 2007, Los Angeles.
Moreb, JS, Baker, VH, Chang, LJ, Lopez, MC and Ostmark, B. Gene expression profiling in
A549 cancer cell line following siRNA mediated knock-down of ALDH1A1 and ALDH3A1.
AACR Annual Meeting, April 2007, Los Angeles.
Chang, LJ. Adoptive immune cell therapy for EBV and CMV infections. Taiwanese
Association of Internal Medicine annual conference. Nov. 2007 Taipei, Taiwan.
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Chang, LJ. Immune effector cell therapy for infectious diseases and cancer. July 6, 2008.
Daopei Hospital, Fifth People s Hospital, Fudan University, Shanghai, China.
Research Personnel (past and present):
Visiting Scientists - Dr. Peter Dickie, Laboratory of Molecular Microbiology, National
Institute of Health, Bethesda, Maryland, USA (6/94-7/94); Dr. Rong-Long Chen, National
Taiwan Univ. Taipei, Taiwan (8/94 -10/94).
Postdoctoral Fellows - Heng-Sheng Fang (6/94-2/95), Yan Cui (10/95-10/99), Tomoo
Iwakuma (7/96-1/99), Zhong-Chen Kou (3/98-1/99), Koichiro Higashikawa (4/99-4/01);
Fumiko Higashikawa (4/99-4/01), Christopher Cogle (MD, 8/99-8/00); Xiaochuan Chen (4/01-
5/05), Xiangguo Liu (2/03-1/02), Shuhong Han (10/04-present), Matthew Cotter (10/06-1/08).
Graduate Students - Chengsheng Zhang, (PhD, 4/94-5/98); Deborah Robinson (PhD, 9/93-
5/98); Ian Parney (MD, PhD, 5/95-10/98); Carlos Chen (MSc, 8/94-11/97); Sumonta
Chaisomchit (PhD, 09/93-9/98); Eric Gay (Ms/MBA, 8/98-5/01); Anne Zaiss (MSc, 2/99-
9/00); Sodany Son (MSc, 8/99-12/02); Jin He (PhD, 05/00-05/05); Bei Wang (PhD, 05/03-
05/07); Ekta Patel (PhD, 05/06-present); Starlyn Okada (PhD, 07/07-present).
Project Students - Brad Booth (2/94-8/94); Shannah Murland (5/94-8/94 Wisest Summer
Research Program); Kathie Walters (6/94-12/94); Ted Chan (9/94-5/95); Deborah Tasa (9/94-
5/95), Marjorie Yang (5/95-8/95); Claudette Rondeau (7/96-5/97); Jason Howard (12/96-5/97);
Anne-Katherin Zaiss (2/99-9/00); Hugh Walters (2/99-10/00, NIH awardee); Andrew Nanton
(1/99-10/00, UF Summer scholarship), Bernice Lo (8/01-4/02), Rimal Patel (9/01-12/01), Jigar
Patel (9/01-12/01), Aurore Lucas (9/01-2/02), Patrick Chang (9/06-2/08), Ander Bergmann
(5/08-present), Kevin Hachey (1/08-present).
SSTP and Summer Students Rebecca Fine (2008), Kyle Jones (2008), Janice Hu (2008),
Jimmy Wong (2008), Yuva Chang (2008).
American Cancer Society Summer Scholar - (2001) Luisa Della Rosa, (2003) Wayne Chou.
UF Scholar - (2003) Katherine Berg.
International Visiting Scholar Liyin Chen (2005), Lily Lien (2006), Liheng Guo (2006),
Yichen Wang (2006), Yuju Huang (2007), Maline Ho (2008).
AHFMR Awarded Summer Students - John Tam, David Sykes (5/94-8/94); Lisa Dyke (co-
supervised with Dr. Stan Houston), James Baughan, Christopher Rudinsky (5/95-8/95).
Research Technicians - Vicki Urlacher (4/95-3/98), Jergen Chua (10/95-3/97), Zhong Fei
(7/96-3/98), and Edward Mason (6/97-5/99), Sodany Son (5/99-12/02), Adeline Deyrieux
(8/01-8/02), Qing Yang (6/02-10/06), Gina Eubank (6/05-10/06), Martin Lu (5/06-5/07),
Victoria Chang (5/07-11/07), Wayne Chou (5/03-7/08), Dane Winner (6/08-1/09), Shanelle
Williams (2/08 present).
17
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