VITAE

Bradley J. Stith abpqh9@r.postjobfree.com

University of Colorado Denver for FED EXP/UPS:

Biology Department (campus box 171) University of Colorado at Denver

PO Box 173364 Biology 171

Denver, CO 80217 1201 Fifth St

Denver, CO 80204)

Tele: 303-***-****

FAX: 303-***-****

EDUCATION

Institution Date Degree Major

Denison University 1970-1971

Ohio State University 1971-1974 B.S. Zoology

Washington State University

1976-1982 Ph.D. Zoophysiology

PROFESSIONAL EXPERIENCE

2001-present Professor, Department of Biology, University of Colorado at Denver.

1994-2001 Associate Professor, Department of Biology, University of Colorado at

Denver.

1987-1994 Assistant Professor, Department of Biology, University of Colorado at

Denver

1982-1987 Postdoctoral Fellow, Department of Pharmacology, School of Medicine,

University of Colorado Health Sciences Center

1982-1986 Instructor, University of Colorado at Denver Continuing Education

Program

1976-1982 Teaching Assistant, Department of Zoology, Washington State University

1

1979 Teaching Assistant, Department of Biology, Stanford University (Hopkins

Marine Station).

1978-1981 Research Assistant, Department of Zoology, Washington State University

1974 Teaching Assistant, Department of Biology, Ohio State University

REFEREED PUBLICATIONS (from 1991, most authors are students)

2008 Douglas W. Petcoff, William L. Holland, Bradley J. Stith. Lipid levels in

sperm, eggs, and during fertilization in Xenopus laevis. Journal of Lipid Research 49:2365-

2378.

2008 Bradley J. Stith. Metformin action on the insulin receptor, other tyrosine

kinases and phosphatases. In Metformin: Mechanistic Insights towards New Applications. Editors:

Gilles Mithieux and Nicolas Wiernsperger. Pgs 59-80. Transworld Research Network publishers,

Kerala, India.

2006 Ken-ichi Sato*, Yasuo Fukami, and Bradley J. Stith.* Signal transduction

pathways leading to Ca2+ release in vertebrate fertilization: lessons from Xenopus eggs.

Seminars in Cell and Developmental Biology. 17: 285-92. *corresponding authors.

2004 Bradley J. Stith. Use of animation in teaching cell biology. Cell Biology

Education 3:8-15, 2004.

2004 William Holland, Thomas Morrison, Ying Chang, Nicolas Wiernsperger,

and Bradley J. Stith. Metformin (Glucophage) inhibits tyrosine phosphatase activity to stimulate

the insulin receptor tyrosine kinase. Biochemical Pharmacology 67:2081-2091.

2003 William L. Holland, Erin C. Stauter, and Bradley J. Stith. Quantification

of phosphatidic acid and lysophosphatidic acid by HPLC with evaporative light scattering

detection. Journal of Lipid Research 44:854-858.

2002 Ayala Luria*, Vaida Vegelyte-Avery*, Brad Stith, Nelly M. Tsvetkova*,

Willem F. Wolkers*, John H. Crowe*, Fern Tablin1, and Richard Nuccitelli.*2 From the

*Section of Molecular and Cellular Biology, University of California, Davis, CA 95616,

1Department. Of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,

University of California, Davis, and the Department of Biology, University of Colorado-

Denver, Denver, CO 80217. Discrete Microdomains in the Plasma Membrane of Xenopus laevis

eggs. Biochemistry. 41:131**-*****.

2000 Thomas Morrison, Leslie Waggoner, Laura Whitworth-Langley, and

Bradley J. Stith. Nongenomic action of progesterone: activation of Xenopus oocyte

phospholipase C through a plasma membrane-associated tyrosine kinase. Endocrinology, 141:

2145-2152.

2000 Stith, Bradley J. Use of a web site to enhance a Biology lecture course.

2

Technological Horizons in Education, 27:20-28.

2000 Stith, Bradley J., Jennifer Hall, Patrick Ayes, Leslie Waggoner, James

Moore, Walt Shaw. Quantification of major classes of Xenopus phospholipids. Journal of Lipid

Research 41: 1448-1454.

1998 Stith, Bradley J., Keith Woronoff, and Nicolas Wiernsperger. Stimulation

of the intracellular portion of the human insulin receptor by the antidiabetic drug metformin.

Biochemical Pharmacology 55:533-536.

1997 Stith, Bradley J., Keith Woronoff, Ronald Espinoza, and Tanya Smart. Sn-

1,2-diacylglycerol and choline increase after fertilization in Xenopus laevis. Molecular Biology

of the Cell 8:755-765.

1996 Stith, Bradley J., Marc L. Goalstone, Ronald Espinoza, Cori Mossel,

Dawn Roberts, and Nicolas Wiernsperger. The antidiabetic drug metformin elevates receptor

tyrosine kinase activity and inositol 1,4,5-trisphosphate mass in Xenopus oocytes. Endocrinology

137:2990-2999.

1994 Stith, Bradley J., Ronald Espinoza, Dawn Roberts and Tanya Smart.

Sperm increase egg inositol 1,4,5-trisphosphate mass in Xenopus eggs preinjected with calcium

chelators or heparin. Developmental Biology 165:206-215.

1993 Stith, Bradley J., Marc Goalstone, Sally Silva and Chris Jaynes. Inositol

1,4,5-trisphosphate mass changes from fertilization through first cleavage in Xenopus laevis.

Molecular Biology of the Cell 4:435-443.

1992 Stith, Bradley J., Marc L. Goalstone and Allen J. Kirkwood. Protein

kinase C initially inhibits the induction of meiotic cell division. Cell Signaling 4:393-403.

1992 Stith, Bradley J., Chris Jaynes, Marc L. Goalstone and Sally Silva. Insulin

and progesterone increase 32P04-labeling of phospholipids and inositol 1,4,5-trisphosphate mass

in Xenopus oocytes. Cell Calcium 13:341-352.

1991 Stith, Bradley J., Allen J. Kirkwood, and Erica Wohnlich. Insulin-like

growth factor 1, insulin and progesterone induce early and late increases in Xenopus oocyte sn-

1,2-diacylglycerol levels before meiotic cell division. Journal of Cellular Physiology 149:252-

259.

1989 Stith, Bradley J. and William R. Proctor. Microinjection of inositol 1,2-

(cyclic)-4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate,and inositol 1,4,5-trisphosphate into

intact Xenopus oocytes can induce membrane currents independent of extracellular calcium.

Journal of Cellular Biochemistry 40:321-330.

1987 Stith, Bradley J. and James L. Maller. Induction of meiotic maturation in

Xenopus oocytes by 12-0-tetradecanoylphorbol 13-acetate (TPA). Experimental Cell Research

169:514-523.

3

1986 Maller, James L., Linda Pike, Gary Freidenburg, Renzo Cordera, Bradley

J. Stith, Edwin Krebs and Jeffrey Olefsky. The phosphorylation of ribosomal protein S6 is

increased following microinjection of insulin receptor-kinase into Xenopus oocytes. Nature(L)

20:459-461.

1986 Maller, J.L., D. Stefonovic, E. Erikson, and B.J. Stith. Regulation of

ribosomal protein S6 phosphorylation by protein-tyrosine kinases. In Current Communications in

Molecular Biology: Translational Control. Ed. by M.B. Mathews, published by Cold Spring

Harbor Laboratory.

1985 Stith, Bradley J. and James L. Maller. Increased intracellular pH is not

necessary for ribosomal protein S6 phosphorylation or meiotic maturation in Xenopus oocytes.

Developmental Biology 107:460-469.

1984 Stith, Bradley J. Biochemical examination of Rana pipiens epithelial

mucus. Journal of Comparative Physiology B. 155:89-96.

1984 Stith, Bradley J. Effects of an external charged layer on transepithelial ion

movement. Journal of Comparative Physiology B. 155:97-101.

1984 Stith, Bradley J. and James L. Maller. The effect of insulin on

intracellular pH and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.

Developmental Biology 102:79-89.

PUBLICATIONS IN PREPARATION OR UNDER REVIEW

In Review: Douglas Petcoff, William Holland, Todd Bergren,Ying Chang and

Bradley J. Stith. Phosphatidic acid may play a central role in fertilization.

In Preparation: Stith, B.J. Phosphatidic Acid Induces Fertilization Events.

In Preparation: Stith, B.J., Ciapa, B. Hydrolysis of Inositol trisphosphate in Xenopus

eggs is accelerated by elevated calcium.

In Preparation: Petcoff, D., Stith, B. Nongenomic action of progesterone in a Xenopus

preparation: progesterone releases calcium from a plasma membrane-cortex preparation.

PUBLICATIONS IN REFEREED MEETING PROCEEDINGS (from 1990, most authors

were students)

2008 Ryan Bates, B. J. Stith. Tyrosine Phosphorylation Changes during

Xenopus Sperm Acrosome Reaction and Fertilization. American Society for Cell Biology, San

Franciso, CA. Page 402 at http://www.ascb.org/files/am08/abstracts/regular.pdf

B. J. Stith,1 J. Juergens,1 J. Snyder,1 R. Bates,1 J. Ash,1 D. Petcoff,2 E.

2007

Cook,2 A. Barkans,2 M. Lintz2; 1Biology, University of Colorado Denver, Denver, CO, 2Biology,

Metropolitan State College, Denver, CO. Phosphatidic Acid (PA) Activates Xenopus Src and

Phospholipase C Whereas PIP Strip Fat Blots Show Src Binds PA. Mol. Biol. Cell 18 (suppl),

4

abstract # 2053 (CD-ROM).

http://www.abstractonline.com/viewer/viewAbstractPrintFriendly.asp?CKey={A6187F58-6F35-

445E-9E82-3AE5F71FD625}&SKey={17A57D52-11D8-4405-A7D4-

A9481015E90C}&MKey={66B05B0E-3C08-4BB1-869F-

AAF586698022}&AKey={088FBDBF-3C4D-4212-865B-3612F7DD115B}

2006 K.-I. Sato, M. Kurokawa, K. Sakakibara, A. K. M. Mahbub Hasan, Y.

Ueda, Z. Ou, T. Iwasaki, B. J. Stith, R. A. Fissore, Y. Fukami. Signal Transduction pathways

leading to Ca2+ release and egg activation in vertebrate fertilization: lessons from Xenopus and

mammalian eggs. Lipid Rafts and Cell Function, Keystone Symposium, March 23-28, 2006.

B. J. Stith,1 J. Juergens,1 J. Stafford,1 J. Snyder,1 M. Wood,2 D. Petcoff2;

2006

1

Biology, University of Colorado Denver, Denver, CO, 2Biology, Metropolitan State College,

Denver, CO. Release of Calcium by Phosphatidic Acid in Xenopus Oocytes and Eggs. Mol.

Biol. Cell 17 (suppl), abstract # 1188 (CD-ROM).

http://www.abstractsonline.com/viewer/viewAbstractPrintFriendly.asp?CKey={92BD1EF9

-138F-44AB-B95C-E33D392E1D82}&SKey={77D20C9B-1B37-4276-AE0D-

BA690A7080A9}&MKey={0C1CA8A7-4052-4C63-8057-

193FDAECCB64}&AKey={088FBDBF-3C4D-4212-865B-3612F7DD115B}

B. J. Stith,1 J. Stafford,1 Y. Chang,1 T. Kane,2 R. Bates,2 A. Nickle,2 D.

2005

Petcoff ; Biology, University of Colorado Denver, Denver, CO, 2Biology, Metropolitan State

21

College, Denver, CO. The Role of Phosphatidic Acid in Regulation of Intracellular Calcium

during Fertilization in Xenopus. Mol. Biol. Cell 16(suppl), abstract#515 (CD-Rom).

http://www.abstractsonline.com/viewer/viewAbstract.asp?CKey={76BDCA05-3D4E-4F99-

91E9-3873C8B31CD2}&MKey={A017DB07-275B-4A9D-833F-

DB2760E62BDA}&AKey={088FBDBF-3C4D-4212-865B-

3612F7DD115B}&SKey={7A0EB81C-EABB-4A87-94C6-3F6C8BDB9A37}

S. Pelech,1,2 G. Cheung,2 H. Paddon,2 X. Shi,2 B. Stith,3 H. Zhang1;

2004

1:Kinexus Bioinformatics Corporation, Vancouver, BC, Canada, 2:The Brain Research Centre,

University of British Columbia, Vancouver, BC, Canada, 3: Biology, University of Colorado,

Denver, CO. Kinetworks Analysis of the Phosphoproteome during Frog Oocyte Maturation.

Molecular Biology of the Cell 15: 26a.

2003 Tseng, N., Chang, Y., Silverstein, T., Szczesny, C., Stith, BJ.

Phosphatidic acid increases IP3 and Induces Calcium-Dependent Events in Xenopus eggs.

Molecular Biology of the Cell 14: 387a.

2003 Stith, B.J. Lipid signaling in fertilization: role of phosphatidic acid. The

FASEB Journal 17:123.6

2002 Holland, W., Stith, BJ. Insulin stimulates meiotic cell division and

phospholipase D activity in Xenopus oocytes. Molecular Biology of the Cell 13:12a.

2001 Holland, W., Stith, BJ. Funding Primarily Undergraduate Research in

Cell Signaling, FASEB Journal 15, A405.

5

2000 Petcoff, D., Holland, W., Stith, B.J.. Phosphatidic acid may play a central

role in fertilization. Molecular Biology of the Cell 11:406a.

2000 Holland, W., Medina, P., Petcoff, D., Savi, K., Stith, B.J. Fertilization and

lipid signaling in Xenopus laevis. Developmental Biology 222:242.

1999 Stith, B.J., Lupe, C., Holland, W., Swise, L. Lipid changes at fertilization

in Xenopus laevis. Molecular Biology of the Cell 10:359a.

1999. Petcoff, D., Medina, P., Savi, K., Morrison, T., Bergren, T., Batbayar, K.,

Stith, B.J. Detection of phospholipase D in Xenopus oocytes. Molecular Biology of the Cell

10:335a.

1998 Waggoner, L., T. Morrison, B. Stith. Regulation of Xenopus

Phospholipase C. Molecular Biology of the Cell 9:119a.

1997 Waggoner, L., Morrison, T., Stith, B. Sphingomyelinase is activated at

fertilization. Molecular Biology of the Cell 8:107a.

1997 Morrison, T. Waggoner, L., Stith, B. Phospholipids from Xenopus eggs,

sperm and zygotes. Molecular Biology of the Cell 8:107a.

1996 Woronoff, K., Whitworth, L., Espinoza, R., Stith, B.J.. Insulin and

progesterone stimulate phospholipase D in Xenopus oocytes. Molecular Biology of the Cell 7:9a.

1995 Mossel, C.A., Woronoff, K., Espinoza, R., Stith, B.J. Activation by

hormone and metformin of IGF-1 receptor in a cortical-membrane preparation from Xenopus

oocytes. Mol. Biol. Cell 6:11a.

1994 Stith, B.J. Protein kinase C initially inhibits then stimulates the induction

of cell division. J. Cell. Biochem. 18D:92. Keystone Symposium, Protein Kinase C.

1994 Smart, T., R. Espinoza, K. Woronoff, D. Roberts, B.J. Stith. Progesterone

or insulin increase IP3 release in cortical preparations, PKC translocation in whole Xenopus

oocytes and maturation is inhibited by buffering intracellular calcium. Mol. Biol. Cell 5:94a.

1994 Roberts, D., C.A. Ferdensi, T. Smart, R. Espinoza, B.J. Stith. PI turnover

after fertilization. Mol. Biol. Cell 5:462a.

1993 R. Espinoza, D. Roberts, T. Smart, B.J. Stith. Sperm addition increases

inositol 1,4,5-trisphosphate mass in eggs preinjected with calcium buffers. Mol. Biol. Cell

4:140a.

1993 B.J. Stith. Changes in inositol 1,4,5-trisphosphate and sn 1,2-

diacylglycerol during meiosis, mitosis and fertilization in Xenopus laevis. J. Cell. Biochem.

17A:289. Keystone Symposium, Phosphorylation/dephosphorylation in signal transduction.

1992 Stith, B., M. Goalstone, C. Jaynes, S. Silva. Insulin and Progesterone

32

increase P-labeling of phospholipids, and DAG and IP3 mass during induction of meiosis in

6

Xenopus oocytes. J. Cell. Biochem. 16B:180. Keystone Symposium, Positive and Negative

Growth Control.

1992 Goalstone, M., and B.J. Stith. Microinjection of Ras p21 into Xenopus

oocytes induced an immediate decrease in sn-1,2-diacylglycerol and a late increase in both DAG

and inositol 1,4,5-trisphosphate mass. Mol. Biol. Cell 3:18a.

1992 Silva, S., M. Goalstone, C. Jaynes, W. Dickerson, and B.J. Stith. Multiple

changers in inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol mass occur from fertilization

through first cleavage in Xenopus laevis. Mol. Biol. Cell 3:24a.

1991 Silva, S., M.L. Goalstone, C. Jaynes and B.J. Stith. Insulin and

progesterone increase 32P-labeling of phospholipids and inositol 1,4,5-trisphosphate mass in

Xenopus oocytes. J. Cell Biol. 115:46a. American Society for Cell Biology, Boston, MA.

1990 Tienda, Y., A. Kirkwood, J. McLemore and B.J. Stith. Addition of

phosphatidylcholine-specific phospholipase C inhibits insulin-, insulin-like growth factor 1-, and

progesterone-induced meiotic cell division in Xenopus oocytes. J. Cell Biol. 111:365a. American

Society for Cell Biology, San Diego, C.A.

1990 Stith, B.J. and E. Wohnlich. Insulin, insulin-like growth factor 1, and

progesterone increase DAG levels at 15 min and immediately before meiotic cell division in

Xenopus oocytes. J. Cell Biol. 111:365a.

1989 Stith, B.J. 1,2-Diacylglycerol Increases at Meiotic Cell Division. Biology

of Cellular Transducing Signals 9:385. Biology of Cellular Transducing Signals, George

Washington University, Washington, D.C..

1988 Stith, B.J., Proctor, W.R. Comparison of Inositol Phosphate Action in an

Intact Cell. J. Cell. Biochem. 12E:112. UCLA Symposium on Cellular Activation and Signal

Initiation, Keystone, CO.

1987 Stith, B., J., Proctor, W. R. The affects of inositol phosphate derivatives on

membrane depolarization in the Xenopus oocyte. Soc. Neuro. Abst. 13:107. Society for

Neuroscience, New Orleans, LA.

1986 Stith, B. and Maller, J. Effects of tumor-promoting phorbol ester on

meiotic cell division in Xenopus oocytes. J. Cell Biochem. 10C:207. UCLA Symposium on

Growth Factors, Tumor Promoters, and Cancer Genes; Steamboat Springs, CO.

1985 Stith, B. and Maller, J. Evidence polyphosphoinositide turnover is

involved in Xenopus oocyte maturation. J. Cell Biol. 101:490a. American Society for Cell

Biology, New Orleans, LA.

1984 Stith, B. and Maller, J. The effect of priming Xenopus laevis females with

pregnant mare's serum gonadotropin on subsequent progesterone-induced oocyte maturation in

vitro. J. Cell Biol. 99:52a. American Society for Cell Biology, Kansas City, KA

7

1983 Stith, B. and Maller, J. The effect of insulin and progesterone on

intracellular pH and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis. J. Cell

Biol. 97:21a. American Society for Cell Biology; San Antonio, TX.

NON-REFEREED PUBLICATIONS

Web publications and presentations from national meetings

(http://carbon.cudenver.edu/~bstith/; most updated on regular basis):

Adjusting to a PUI after a research institute or medical center

Use of web animation and video in teaching

Use of the popular movie "Lorenzo's Oil" in teaching Cell Biology

Use of WebCT for a web site for a lecture course

Funding Sources for Undergraduate Research

How To: Grant Writing

How To: Use of NSF s FASTLANE submission process

Web animation and video in teaching

1999 Stith, BJ. Proposal Advice, published in the Research Advocate,

Office of Sponsored Programs. Vol. 17, 12, June 1999.

COURSES TAUGHT

Introduction to Research

General Biology

General Cell Biology

Advanced Cell Biology

Developmental Biology

Cell Signaling

Cell Biology laboratory

RESEARCH AND EDUCATION PRESENTATIONS AT MEETINGS AND SEMINARS

2008 Invited Plenary Speaker. Educational Aspects and Development of

Research programs in nonPhD institutions. Clarion University (PA). October.

2008 Invited Plenary Speaker. Development of Undergraduate-based Research

8

Programs. Northern Michigan University. February.

2007 Invited Speaker, University of Colorado Denver, Cancer Center at

Anschutz Medical Center. Phosphatidic Acid (PA) Activation of Src and PLC : Lipid

Regulation of a Tyrosine Kinase. November.

2007 Lipomics Workshop at Experimental Biology 2007. Washington, D.C.

Overview of protocols developed by LIPID MAPS Consortium for Lipidomic analysis. Poster

Presentation: HPLC and Evaporative Light Scattering Detection for LIPOMICS During

Fertilization. April.

2007 Invited Speaker, National Science Foundation, Cell Biology group; April.

Phosphatidic Acid May be a New Ca++ Regulator during Fertilization.

2006, 2008 Program Organizer: Grant Writing Workshop, with Ami Ahern-Rindell,

University of Portland, and Sally O Connor, National Science Foundation. National Conference,

council on Undergraduate Research, DePauw University, June.

2005 Invited Plenary Speaker, Faculty Forum on Undergraduate Education at

Southern Connecticut State University, New Haven, CT. Topic: The Use of Undergraduate

Research in Teaching.

2004 Invited speaker, University of South Florida Medical School, Dept. of

Biochemistry, Mechanism of anti-diabetic drug metformin, November.

2004 Invited speaker, MBRS SCORE program, Colorado State University-

Pueblo, Lipid analysis: mechanism of fertilization. November.

2003 Program Organizer and Speaker, Role of Phosphatidic acid in

Fertilization, Amphibian Fertilization meeting, American Society for Cell Biology, San

Francisco, CA. December.

2002 Invited speaker: Membrane fusion at fertilization by phosphatidic acid;

American Society for Cell Biology, San Francisco, CA. December.

2002 Phospholipase C Activation by progesterone; FASEB Summer conference

on Phospholipases, Tucson AZ, June.

2002 Invited speaker, national meeting of Council on Undergraduate Research,

"Obtaining funding at Primarily Undergraduate Institutions. Research Poster: Lipid Signaling

in fertilization. National meeting of the Council on Undergraduate Research, Connecticut

College, CT.

2002 Poster presentation at Regulation of Cellular Responses by Lipid

Mediators, Keystone conference, Taos, NM. February.

2001 Invited speaker, Phospholipase D and PA regulation of Phospholipase

C. FASEB summer conference on phospholipase D, Tucson, AZ.

9

2001 Invited Speaker, "Undergraduate Research," FASEB meeting, American

Association of Anatomists, Orlando FL, March.

2001 Invited Speaker, "Lipid Signaling in Development." Biology Department,

University of South Florida, Tampa FL, February.

2000 Platform talk ; "Use of a web sites, animation and video in college

teaching." At the annual meeting of the National Association of Biology Teachers, Orlando FL,

October.

2000 Four presentations at the national meeting for Council on Undergraduate

Research at College of Wooster, Wooster OH (1) Adapting to a PUI after a Medical School

PostDoctorate; a workshop I set up with Dr. E. Levy of UCD chemistry, and Dr. M. Coussons-

Read of psychology, (2) Biology grant writing workshop with Mary Jane Saunders of the NSF

and M. Brodl, (3) Use of technology in teaching; a workshop I set up with Malcolm Campbell of

Davidson, and (4) Students and Undergraduate Research (setting up a student research club);

June 21-24.

2000 Presentation on "Teaching with Flash Web Animation and Digitalized

Video" at the Teaching with Technology Conference at the University of Colorado- Colorado

Springs; July 14.

2000 Keystone Conference on Lipid Signaling; Feb. 5-10; Lipid signaling

during Fertilization.

2000 Invited speaker, Department of Cell Biology, University of Colorado

Health Sciences Center, spring, 2000. PI turnover in meiotic cell division and fertilization.

2000 Talk to Biology graduate students; "How to handle the difficult student."

August.

2000 Invited talk on "Bioinfomatics and biological structure" to Dr. Greenberg's

graduate class in mathematics (Math 4/5779). August 28.

2000 Invited speaker, Retreat for Department of Pharmacology, University of

Colorado Health Sciences Center, Copper Mountain, CO. October 6.

1999 Invited speaker (by Dr. E. Nuhfer), Teaching with Technology

Conference, School of Mines, July 13-15, 1999. Use of a Web Site and WebCT to Enhance a

Biology Course.

1999 Stith, B.J., Presentation at FASEB Summer Conference on Protein

Kinases and Phosphorylation. July 24-29.

1998 Invited speaker (developed presentation and part of panel discussion),

Ambiguity and Uncertainty in the 21st Century, March 27, sponsored by Chancellor's Scholars

and Leaders and IBM.

10

1998 Invited speaker at the "International Conference on Xenopus and

Amphibian fertilization;" Sardinia, Italy, September. Talk: "Lipid Signaling in Xenopus

Fertilization." Meeting sponsored by the University of Milan, Italy.

1998 Invited presenter at the University of Colorado Reagents meeting (Jan.

22). Presentation on research involving undergraduates.

1998 Invited speaker at University of Lyon, "The action of metformin." Sept.

1998 Invited speaker and panel member at the Chancellor's Scholars and

Leaders meeting on setting up a science "Discovery Room" (Research connections display;

interactive learning). December 3.

1997 Attended Gordon Conference on "Fertilization and Activation of

Development," August, Holderness School, New Hampshire. I gave a poster/paper on the role of

phospholipids in fertilization.

1996 "Mechanism of induction of cell division by IGF-1." Fifth International

Insulin and IGF Symposium, Univ. of Florida, March 3-5.

1996 Keystone Conference on "Lipid Signaling," March, Taos, New Mexico. I

gave a poster/paper on the role of protein kinase C in the induction of meiotic cell division.

1996 International Xenopus Conference, August, Estes Park, CO. I gave a

poster/paper on the role of phosphatidylinositol turnover in the induction of meiotic cell division

and in fertilization.

1996 Brandt Gillum, Melanie Overley and I put together a poster presentation

for the first UROP/OSCAR poster session held during the fall of 1996. My students and I have

presented at every UROP poster session since their inception.

1996-1998 Presentation for new Biology graduate student orientation meeting.

1996-1998 Presentation for incoming faculty at the University of Colorado-Denver

Faculty Orientation (at request of Dr. F. Baca).

1995 "Phospholipid second messengers after fertilization of Xenopus eggs."

Gordon Research Conference, Plymouth, NH. July 30-August 4.

1995 "Mechanism of Induction of Meiosis in Xenopus oocytes by IGF-1."

International Symposium on IGF-1 action on Ovarian Cells, Montpellier, France, Sept. 15-16.

1995 "Progesterone and elevated calcium stimulate tyrosine kinase in a

membrane-cortical preparation from Xenopus oocytes." Special "Hot" poster session, American

Society for Cell Biology, Washington, D.C., Dec. 9-13.

1992 Invited speaker at American Society for Cell Biology; "Involvement of

Undergraduate students in the Research Lab"

11

1987 Stith, B., Maller, J. Involvement of protein kinase C but not inositol

phosphates in meiotic cell division. FASEB Summer Conference on Intracellular

Calcium, Bellows Falls, VT.

1985 Stith, B., Maller, J. Intracellular pH does not regulate cell division.

International Society of Differentiation; Heidelberg, Germany.

1980 Stith, B.J. Intracellular pH increases during maturation of Patiria miniata.

Northwest Society for Developmental Biology; Friday Harbor, WA.

RECOGNITIONS, HONORS, ETC.

2008, 2006, 2004, 1994-1998 Panel Member, Signal Transduction grant proposal review

panel, National Science Foundation

2008 Faculty Award for Outstanding Student Mentoring, Research and Creative

Activities Symposium. April 11, 2008.

2007 Excellence in Service Award, College of Liberal Arts and Sciences,

University of Colorado at Denver and Health Sciences Center

2005-Present Invited author and reviewer of questions for the Biochemistry, Cell and

Molecular Biology and Biology Subject tests of the Graduate Record Exam.

2006 Invited author, clicker questions for chapters 1-5 of the textbook World

of the Cell. Benjamin-Cummings Publishing.

2005-Present Invited collaborator in the Virtual Cell Project at

http://vcell.ndsu.nodak.edu/animations/ (use of computer animation in higher education;

collaborator on a National Science Foundation CCLI grant submitted January 2006, contributor

to an article on the project for NSF s Discoveries magazine at http://www.nsf.gov/discoveries/).

2005-Present Associate Member, University of Colorado Cancer Center

2004- Present Reviewer of textbooks, Benjamin Cummings (scientific publisher)

2005 Outside Reviewer, Biology Department of Juniata College, Huntingdon,

PA.

2004 Outside Reviewer, Master s program for Applied Natural Sciences,

Colorado State University at Pueblo, CO.

2004 Outside Reviewer, Biology Department, Appalachian State University,

Boone, NC.

2004 Chair of outside review committee for the Biology Department at

Colorado State University at Pueblo, January.

12

2001-Present Invited facilitator, Council on Undergraduate Institute on Proposal Writing

Institute, various locations around USA. July of each year.

2001 Grant reviewer, National Science Foundation, Course, Curriculum and

Laboratory Improvement Program. July.

2000 Chair of outside review committee for the Biology Department at the

University for the Sciences at Philadelphia, PA. Sept. 28-29.

2000 My web site paper of adrenoleukodystrophy is recognized as one of the

the "best layman" summaries of the disease. In 2002, the Myelin Foundation linked it s web site

to mine.

1999-2005 Invited associate editor for Biology of Reproduction, March.

1999 National Science Foundation, Collaborative Research in Undergraduate

Institutions Grant Review panel member.

1999 National Science Foundation, Course, Curriculum and Laboratory

Improvement Grant review panel member.

1998 Invited reviewer by Dr. W. Becker; "World of the Cell" textbook (the

most popular college-level Cell Biology text used in the United States).

1997 Dr. J. Sando (Professor, Pharmacology, University of Virginia Medical

School) spent the fall semester in my lab. She came to learn about extraction of phospholipids,

their separation by HPLC and detection by an evaporative light scattering detector. As she could

have gone to other labs yet chose mine, this was a compliment to our work.

1996 Dr. B. Ciapa (Univ. of Nice, France) came to my lab during the summer.

She collaborated on a series of experiments that examined the breakdown of IP3. As she could

have gone to other labs around the world, yet she chose my lab, this was a compliment to our

work.

1986-1987 American Heart Association Fellowship,

1985-1986 National Institutes of Health Postdoctoral Traineeship,

1981 Cystic Fibrosis Foundation Fellowship,

1981 National Institutes of Health Predoctoral Traineeship,

1974 Cum Laude degree, Ohio State University

1970-1971 Scholarship, Denison University, Granville, OH

PROFESSIONAL ORGANIZATIONS

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American Society for Cell Biology

National Association of Biology Teachers

Council on Undergraduate Research

Society for Developmental Biology

American Society for Biochemistry and Molecular Biology

OTHER INDICATORS OF SCHOLARSHIP

Research Support

2008 Combined Theoretical and Experimental Search for the Acting

Mechanism of the Commonly-Used Antidiabetic Drug Metformin and Derivatives. National

Institutes of Health, R 15 AREA. coPI: Hai Lin (Chemistry). Submitted October 2008.

Requested: $225,467. Pending.

2007-2010 MRI: Acquisition of a Nanoflow Hybrid Triple Quadrupole/Linear Trap

Mass Spectrometer System for Three Diverse Institutions. $469,227. National Science

Foundation. Co-PI: Karen Jonscher.

2006- 2009 AREA program grant; Role of Phosphatidic Acid in the Sperm Acrosome

Reaction. $214,500. National Institutes of Health.

2006 Undergraduate Research Opportunities Grant, with students Jeffery Taylor

Juergens, Karina Bogdasarova, and Brandon Mauracher ($3000).

2005 Undergraduate Research Opportunities Grant, with students Dan Crona

and Afsaneh Manshadi ($2000). Master s Student Grant with Jason Stafford ($1750).

2001-2004 National Science Foundation grant; Lipid Signaling in Fertilization;

Developmental Mechanisms and Signal Transduction; $350,000.

2004 Undergraduate Research Opportunities Grants, $1100 and $1000,

University of Colorado at Denver. One with undergraduate Thomas Arthur, one with Yaser

Namvargolian.

2002 Research support from Lyonnaise industrielle pharmaceutique (LIPHA)

on the mechanism of action of the antidiabetic drug metformin, $40,000 from 1992-1998; in

1999: $14,500; in 2000: $14,000; 2002, $18,000.

2002 Co-PI on $2 million IGERT grant application to the NSF to support

bioinformatics program (multinstitutional program). Not funded.

2001 Faculty grant, mechanism of metformin, University of Colorado-Denver,

$5,000.

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2000 Awarded travel support to present a talk on teaching with technology,

National Association of Biology Teachers, October. Support from Dr. E. Nuhfer.

2000 Undergraduate Research Opportunities Program grant (with Erinn Stauter;

$1150).

1999 Undergraduate Research Opportunities Program grant (with William

Holland)

1998 Undergraduate Research Opportunities Program grant ($2000; funding for

support for 3 students and supplies).

1998 Awarded travel support to attend a conference on teaching with computers

(Educom, Oct. 1998). Support from Drs. E. Nuhfer and C. Pletsch.

1997, 2000 Obtained internal funding for page and publication charges ($668; $500)

from the Office of Sponsored Programs (Dr. F. Baca).

1997-2000 National Science Foundation Grant, Role of Phospholipase D in

fertilization, $280,000.

1996-1997 National Science Foundation Grant, Role of Phospholipase D in

fertilization, $93,000.

1994-1999 Undergraduate Research Opportunity Program grants, University of

Colorado-Denver, sponsor of student grants totaling $8,150.

1990-1999 Summer Supplement for the National Science Foundation grant, for

student stipend and research supplies, totaling $75,000

1993-1996 National Science Foundation Grant, Induction of cell division by protein

kinase C, $362,356,

1990-1993 National Science Foundation Grant, Mechanism of action of the ras p21

protein. $221,088.

1989 Junior Faculty Development Award, University of Colorado-Denver

$4,500.

1987 Junior Faculty Development Award, University of Colorado-Denver,

$4,500.

Other activities

2008 Moderator and Sponsor, with Dr. Mary Reyland, for Cancer Center talk by

Richard Kolesnick, MD; Professor, Department of Molecular Pharmacology & Chemistry,

Sloan-Kettering Institute at Memorial-Sloan Kettering Cancer Center at Anschutz Medical

Center; Tuesday, April 15th, 2008

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2008, 2007, 2006, 2005 Outside reviewer for HHMI Interdisciplinary/Collaborative

Research Fellowships, Canisius College

2005 Due to two student nominations, selected for inclusion in "Who's Who

Among America's Teachers, 2005."

2005 Attended The Undergraduate Experience summary of student surveys

presented by Dr. Richard Light (Harvard). Sept. 23, 2005.

2003-Present With support from a community grant to Dr. L. Edwards, I conducted lab

demonstrations on fertilization with groups of 3 to 8 local science teachers.

2003-1999 Through support of an NSF E2020 grant, each summer about 3 high

school teachers worked in my research lab.

2001 With funding from UCD Office of Teaching Enhancement, attend course

on developing animations for teaching (Macromedia Flash software). Compuskills, Dec. 10, 18.

2001 Attended "Brain Based Learning" teaching seminar; given at UCD by Dr.

Robert Leamnson (Univ. of Mass-Dartmouth), Feb. 16.

2001 Invited reviewer of Dr. W. Becker's World of the Cell textbook. Feb.

1999-2000 Developed two new courses: Developmental Biology and Advanced Cell

Biology (while continuing to teach General Cell Biology and Cell Signaling)

1999 Among the first Web-enhanced lecture course in the College of Liberal

Arts and Sciences: Developmental Biology. I used WebCT to develop this web site for the

course; the software allows posting of student grades, email, bulletin boards, direct use of web,

illustrations and videos. In 2000, I used Blackboard software to develop web site for Advanced

Cell Biology 4054-5054 (a new course), and subsequently switched to Blackboard.

1999 Participant; Delphi study on use of technology in teaching.

1999 Participant, Focus group, UCB, Online learning and Research, Net

Library, Sept.

1998 I attended workshops on teaching with world wide web and the use of

Powerpoint in classrooms (Oct. 22, 1998; and Oct. 27, 1998). I then incorporated use of these

techniques in Cell Biology.

1998 Attended program on Teaching and Technology teleconference; Feb. 10.

This material was used to develop Cell Biology and Developmental Biology.

1998 Attended program on "Becoming an OSCAR Winning Teacher" by Tony

Grasha; Feb. 19. Sponsored by Dr. Ed Nuhfer.

1998 Attended program on "Mentoring Students To Higher-Level Thinking" by

Dr. M. Pavelich, School of Mines. Material applied to Cell Biology course.

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1998 Attended National Conference for the Council on Undergraduate

Research, Occidental College, Los Angeles, July. Support for travel from the Chancellor's Office

and Office of Sponsored Programs.

1998 Due to student nomination, selected for inclusion in "Who's Who Among

America's Teachers, 1998."

1998 My web site and summary of online instruction has been used by Dr. M.

Karpen, Dr. Jensen, Trinity University, by Dr. W. Mech Dean of the honors program at Florida

Atlantic University, by the Council on Undergraduate Research, by Dr. W. Wasserman at

Loyola, and by Dr. E. Ferroni, Benedictine University.

1997 Developed my web site for both teaching and research (a web site that has

had over 6000 hits by year 2000). The web site includes illustrations, course information, papers

published, videos and animation of biological processes. It has been used by many other

professors at other institutions (who teach similar courses, or perform similar research) and

acknowledged by (and linked to) web sites for "Xenopus one" (a web site used by researchers),

the Society for Developmental Biology, and The Myelin Foundation.

1997 Attended program on Enhancing and Evaluating Teaching Performance; a

video conference with Peter Seldin (Nov. 14). Sponsored by Ed Nuhfer.

1997 Attended program on "Becoming an OSCAR Winning Teacher" video

conference on Friday, Oct. 31. Sponsored by Ed Nuhfer.

1997 Worked with Carl Pletsch on use of animations in classroom; obtained

$2900 Laptop computer for department teaching.

1997 I introduced the use of CD-ROMs and animation in the teaching of

subjects poorly shown by static illustrations (DNA replication, transcription and translation) in

lectures of General Biology, and later, Cell Biology 3611.

1992 Attended National Association of Biology Teachers meeting and

participated in discussions on teaching General Biology.

1993 Attended "Council on Undergraduate Research Dialog;" a meeting with

the National Institutes of Health, National Science Foundation and other funding agencies to

discuss research at predominately non-Ph.D.-granting institutions.

1990-Present Attend annual Symposium on Teaching Cell Biology at the American

Society for Cell Biology meeting.

1990-Present. Attend yearly Symposia on Improvement of Teaching skills at the

University of Colorado at Denver. In 2000, I suggested the theme of the meeting and helped

organize it.

1990 Attended Workshop on Academic Research Enhancement Award (AREA)

Program at the National Institutes of Health, Washington, DC. Report to Office of Sponsored

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Programs on research support for smaller institutions.

1978, 1979 Developmental Biology Course, Hopkins Marine Station, Stanford

University (1978 student; 1979 selected to be a Teaching Assistant in the course).

SERVICE

Department of Biology

2008-2009 Chair, Search Committee, Developmental Biology position.

2007-2008 Chair, Search Committee, Biological Sciences Educator position

2003-2004 Chair, Search Committee, Molecular Biologist position.

2001 Chair, Search Committee, Physiology position

1999 Search Committee member, Lab Coordinator.

1999 to 2004 Department secretary for faculty meetings

1999 to present Mentoring Committees for junior faculty

1998 Search Committee member, Physiology position

1998 I summarized and circulated to Biology department faculty a summary of

a discussion held at the American Society for Cell Biology on teaching Biology and the future of

Biology departments.

1998 I conducted two surveys in Cell Biology 3611 concerning issues in

teaching and in computer skills. The surveys have been distributed to faculty and others involved

in teaching with technology.

1994 Tenure Track Job Search Committee, Plant Physiologist

1992 Search Committee Member, Biology lab coordinator

College of Liberal Arts and Sciences

2002 to 2007 Member, Oversight committee, Certificate Program in Computational

Biology.

1989-1995 Member of College of Liberal Arts and Sciences Council.

2000 Animal Quarters space committee

2000 Math Core Curriculum committee

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University

1988-Present Member and, in 1991, Chair of Institutional Animal Care and Use

Committee.

2008 Member, Vice Chancellor s Advisory Committee for Retention, Tenure

and Promotion.

2005-2008 Chair, Undergraduate Research (Grant) Opportunity Program Committee

2005-2007 Member, Committee for the Research and Creative Activities Day

2005 Member, Experiential Learning Committee

2005 Member, Undergraduate Research/Science Planning Committee

2004 Member, UCD-UCHSC merger team

1999-2002 Faculty Assembly Academic Personnel Committee

1999-2001 Academic Standards Committee

2000-2002 Educational Planning and Policy Committee

2000-2002 Goldwater Scholarship Committee

1999 Panel member, Successful Strategies for Winning Grants, workshop

sponsored by Vice Chancellor and Office of Sponsored Research Feb. 18.

1999 Committee member, University of Colorado-Denver Teaching committee;

initiated idea for theme of annual teaching seminar (held Feb, 2000).

1998 Member, Peer Advisory Board, Informed Citizen's Quarterly (publication

of Chancellor's Scholars and Leaders Program)

1988 Report to Office of Research Administration on undergraduate research

and funding based on attendance at the National Council on Undergraduate Research Conference

at Carleton College, Northfield, MN.

1998 Invited member of the "Informed Citizen's Quarterly" (journal of the

Chancellor's Scholars and Leaders); meeting on Dec. 1, 1998.

Local

2008 Presentation to Scouts at Salem United Church of Christ (Denver),

showing videos taken through microscopes (e.g., white blood cells crawling from a blood vessel

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to a cut in the skin). Scoutmaster Jim Reagan said that the ~20 kids and about 25 adults paid

more attention than when Hawk Quest came! (September 19)

2004, 2005 Team leader, Destination Imagination, Eastridge Elementary and Prairie

Middle School

1999-2006 My research lab hosted High School Biology teachers through the NSF

E2020 program or through a local Boetcher Foundation grant.

2003-2006 Consultant to local Rose Foundation grant for enhancing high school

teacher skills

1999 Committee member, hiring of Teacher, Rocky Mountain School of

Expeditionary Learning (public school supported by 4 districts). May.

1997 Advised Steve Hall of Jefferson County Magnet School on Cell Biology

Federal

2008, 2006, 2004, 1994-1998 Panel Member, National Science Foundation, Signal Transduction

and Regulation Grant Review Panel.

2001, 1999 Panel Member, Course, Curriculum, and Laboratory Improvement grant

review panel. National Science Foundation. July.

1999 Panel Member, Collaborative Research in Undergraduate Institutions grant

review panel. National Science Foundation. Two meetings in Washington D.C. in spring.

Industry

1992-2004 Consultant on mechanism of action of anti-diabetic drug metformin to

manufacturer Lyonnaise industrielle pharmaceutique (LIPHA).

1999-Present Collaborator with Avanti Polar Lipids, Inc. (Alabaster, AL) on new lipid

analysis methodology.

Professional Associations

2001-Present Invited facilitator, Council on Undergraduate Institute on Proposal Writing

Institute, various locations around USA. July of each year.

2005 Local Arrangements Committee, Council on Undergraduate Research

Proposal Writing Institute, Colorado State University-Pueblo, July.

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2003-2005 Chair, Council on Undergraduate Research Finance Committee.

1996 to Present Annual meetings of the Council on Undergraduate Research: governing

body, grant writing presentation organizer, and research poster presentation. June.

2002-present Reviewer, abstracts for Council on Undergraduate Research Posters on

the Hill; Washington DC, January.

1998-present Biology Councilor for the Council on Undergraduate Research

1998-2005 Member, Council on Undergraduate Research Finance Committee.

1998 Service to "Council on Undergraduate Research": paper on online

teaching.

1997-present Nominated by Dr. Randall Tagg (Physics) to position of Biology

Councilor for the Council on Undergraduate Research (elected early, 1998).

1997 Service to Council on Undergraduate Research: paper on content of

departmental web sites.

1992 Local Arrangements committee for the national meeting of the American

Society for Cell Biology.

1988-1997 Outside reviewer for United States-Israeli Binational Science Foundation.

1987-Present Reviewer for various journals (e.g., Development, Diabetologia

Developmental Biology, Biochemical Pharmacology,, Journal of Experimental Zoology, Life

Sciences Education/Cell Biology Education, etc.) and granting agencies (National Science

Foundation, Israeli Bi-national Grant program, Murdock Trust, Veteran's Medical Association).

Past Associate Editor of "Biology of Reproduction and Cell Biology Education/Life Sciences

Education.

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