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Topic: Resume for Sabeen Raza, Sound experience in Biotechnology and Bioinformatics (Read 10787 times)

Posts: 1

« on: 01 February 2002 10:48:03 AM »

S Raza

E-Mail: *******@***.***

OBJECTIVE

Seeking a long-term career as integral member of a R&D team in a Biotechnology setting allowing me to utilize my talents and skills to contribute toward research and development in Biological Sciences.

EMPLOYMENT HISTORY

Oct 2002 - Present Department: INFORMATICS; Bacterial Barcodes Inc. Houston, Tx

Bacterial Barcodes is a molecular diagnostic company that specializes in the development and standardization of automated molecular typing for epidemiological and classification studies of microbial pathogens.

Accomplishments at Bacterial Barcodes:

Informatics:

* Supported in designing and development of the DiversiLab System Software

* Designed and developed the Laboratory Information Management Systems (LIMS)

* Helped design and develop MYSQL database schema and architecture for the new version of DiversiLab System Software.

* Helped in the development of user interface of the new version of the DiversiLab System software.

* Carried out Installation Qualifications (IQ) and Operational Qualifications (OQ) for installation of new customer sites under QSR.

* Validation and testing of the software during development.

Technical and Laboratory Work:

* Developed strain and species-specific DNA fingerprinting kits for molecular typing of various pathogenic microbes using repetitive sequences based PCR (repPCR) and microfluidics technology.

* Wrote SOP for making BBCI libraries, writing training manuals and user guides under QSR.

* Training in laboratory practices (Development and QSR)

* Submitted abstracts in the form of posters for presentation at various scientific meetings and conferences including ASM, ASM Bio-Defence, ICAAC, IMEMM and ISHAM.

* Helped in primer search and design using various sequence analysis software and tools

Marketing:

* Developed (using HTML) and launched the company's corporate website * Created images and figures for company brochures and other marketing documents.

* Wrote training manuals and user guides for the DiversiLab System software under QSR.

* Wrote technical notes and application profiles under QSR.

* Helped produce Power Point presentations for educational, informational purposes.

* Created announcement poster boards and flyers for tradeshows and other Marketing purposes.

* Gained a working knowledge of the contact database ACT! 6.0

July 1998 - Oct 1999

Department: INVENTIONS; Syngenta (previously known as Zeneca Agrochemicals).

Worked in the Plant Biotechnology Section of the department. Duties included the verification of the integrity of clones being produced for transformation into plants using Molecular Biological methods and bioinformatics packages. Designed and developed MS-Access relational database to store information about clones collected from the experimental officers in the department.

Feb 1998 - July 1998

Department of Molecular Genetics at the Institute of Ophthalmology.

Worked as a research assistant for a Scientific Officer. The project involved working on a corneal dystrophy called Congenital Hereditary Endothelial Dystrophy (CHED), which had previously been mapped to a region on chromosome 20p11.2. Duties also included the physical mapping of the disease locus.

Sept 1995 - Aug 1996

Joint Microbiology Research Unit; King's College School of Medicine and Dentistry.

Routine work involved the taxonomy of the two main genospecies of A. naeslundii by Microbial and Molecular Biological methods. Main Responsibilities included isolating single colonies of A naeslundii, isolating the chromosomal DNA, running RFLP analysis on the restriction digests to detect the differences in the RFLP patterns of the different strains within the same genospecies. Further identification of the different strains within the same genospecies was carried out by PCR.

EDUCATION

Oct 2000 - August 2002

Birkbeck College, University of London,

MSc in Molecular Modelling and Bioinformatics

Project Supervisor: Dr Sylvia Nagl

Title of project: Computational search protocol for the characterisation of proteins.

Sept 1993 - July 1997

University of East London,

BSc. (Hons) in Applied Biology

Project Supervisor: Dr David Rowley

Title of project: Comparing the number of hydrogen peroxide producing and non-producing Lactobacilli from HVS of women suffering from bacterial vaginosis.

COMPUTER SKILLS

Operating Systems

Windows/DOS, Windows NT, Unix/Linux

Programming Languages

Perl-CGI, Java Script (DHTML)

Markup Languages

HTML, XML

Modelling Software

AMBER, GROMEX, Rasmol/Chime, Sybyl/O, Quanta/CHAARM

Databases

SQL, MySQL, MS Access

Bioinformatics Packages

Vector Nti suite, Sequencer, Map Draw, Enhance, Meg Align, Primer Select, Macaw, Seqman, Macvector, Geneworks

Search Tools

Gopher, MedLine, BIDS, SRS, Zsearch, BLAST(n,p&x), Expasy (SWISS-PROT & TREMBL), Interpro, Pfam

her skills

- Competent in the loading and running of repPCR amplicons on Microfluidics chips (LabChip(tm));

- Analysis and interpretation of electropherogram signals from the Agilent 2100 Bioanalyzer for bacterial strain typing;

- Understanding of R (Statistical package) for cluster analysis;

- In depth understanding of various clustering analysis and distance matrices including UPGMA, Pearson coefficient and dice co-efficient.

LABORATORY SKILLS

* Expertise in chromosomal and plasmid DNA isolation from bacteria and fungi (Mini, Midi and Maxi preps) and

* Human genomic DNA from peripheral blood samples;

* DNA quantification using various methods; using Hoescht Dye method and Spectrophotometry

* Positional cloning techniques, involving the isolation of YACs from YAC libraries

* Pulse Field Gel Electrophoresis (PFGE)

* Expertise in Polymerase Chain Reaction (PCR)

* Expertise in repPCR (repetitive element based PCR)

* STS content mapping by PCR

* Competent in RFLP analysis

* Northern and Southern blotting

* Expertise in agarose gel electrophoresis

* Competent in Column chromatography

* Confocal microscopy

* Some experience with various Immunological techniques such as ELISA and RIA

* Non radioactive sequencing using ABI technology

PUBLICATIONS

Healy, M., Huong, J., Raza, S., Lising, M., Woods, C., Versalovic, J., Lupski, J. Applications of automated microbial DNA fingerprinting using rep-PCR. In preparation for the Journal of Clinical Microbiology.

PUBLISHED ABSTRACTS

1) Raza, S., Huong, J., Bittner, T., Healy, M. Identification and Strain Differentiation of Food and Waterborne Bacterial Threat Agents Using Automated rep-PCR. Presented at ASM Biodefense conference, 2003

2) Huong, J., Raza, S., Healy, M. Identification of Pathogenic Escherichia coli Using automated rep-PCR. Presented at ASM 103rd conference, 2003

3) Healy, M., Webb, R., Manry, J., Huong, J., Raza, S., Reece, K., Bittner, T., Lising. M., Shah, K. Towards a more universal system of microbial typing. Presented at ICAAC 43rd conference, 2003

4) Huong, J., Bittner, T., Webb, R., Lising, M., Manry, J., Reece, K., Raza, S., Healy, M., Shah, K. Differentiation of Aspergillus species collected from hospitals and environment by automated rep-PCR

5) Reece, K., Bittner, T., Raza, S., Walton, D., Healy, M., Shah, K. Aspergillus speciation and strain discrimination; a comparison between automated rep-PCR-based profiling and 28s rDNA sequencing. Presented at IMMEM 6th conference, 2003

6) Reece, K., Huong, J., Webb, R., Manry, J., Raza, S., Bittner, T., Healy, M. Differentiation of Aspergillus species using automated rep-PCR DNA fingerprinting. ISHAM 50th, 2003

REFERENCES

1. Dr Mimi Healy, Chief Scientific Officer and Vice President, Bacterial Barcodes Inc. 8080 North Stadium Drive, Ste 1200, Houston - Tx. 77054, Tel: 713-***-****. E-mail: ******@***********.***

2. Dr Keith Steward, Director of Bioinformatics, Bacterial Barcodes Inc. 8080 North Stadium Drive, Ste 1200, Houston - Tx. 77054, Tel: 713-***-**** ext 155. E-mail: ********@***********.***

3. Dr Sylvia Nagl, Department of Biochemistry, University College London (UCL), Gower Street, London, Tel: +44-207-***-****. E-Mail: ****@************.***.**.**

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