Project Assistant

Curriculum Vitae

Name: Srinivas Tipparaju

USF College of Pharmacy

Address:

***** ***** *. ***** ****. Box MDC030

Tampa, FL

Email: abph7v@r.postjobfree.com

United States Permanant Resident

Certifications and Registration:

Foreign Pharmacy Graduate Equivalency Certificate (FPGEC) certification, conducted by

National Association of Boards of Pharmacy (NABP), EE# 208-4286

Registered as Pharmacist Intern with Florida Board of Pharmacy (#PSI28134).

Educational Qualifications:

2003: Post-doctoral fellow, Emory University, Atlanta, GA

2002: Ph.D. Central Drug Research Institute, India.

1998: Master of Pharmacy, Birla Institute of Technology and Science (BITS), Pilani, India.

1996: Bachelor of Pharmacy, Nagpur College of Pharmacy, India.

Positions and Honors

Positions and Employment

May 2011- present: Assistant Professor, Department of Pharmaceutical Sciences, College of

Pharmacy, University of South Florida, Tampa, FL

Jan2012-present: Assistant Professor, joint appointment Department of Molecular Medicine,

Morsani College of Medicine, USF Health, Tampa, FL

July 2010-April 2011: Assistant Professor of Medicine, University of Louisville, Louisville, KY

July.2008-June 2010: Instructor, Division of Cardiovascular Medicine,

Department of Medicine, University, KY

Dec.2003-June.2008: Research Associate, Division of Cardiology,

Department of Medicine, University of Louisville, Louisville, KY

Jan.2002-Nov.2003: Post-doctoral fellow, Todd Franklin Cardiac Research Laboratory,

Department of Pediatrics, Childrens Research Center, Emory

University, Atlanta, GA

April 1998-Sept.2001: Senior Research Fellow (Council for Scientific and Industrial

Research, New Delhi, India), Division of Pharmacology, Central Drug

Research Institute, Lucknow, India

July 1997- Dec.1997: Practice School trainee (Birla Institute of Technology and Science),

Torrent Pharmaceutical Research Center, Gandhi nagar, India

Administrative and academic roles:

Course Co-ordinator: Drug Delivery Systems-I and II, USF Health College of Pharmacy

Laboratory Co-ordinator: Drug Delivery systems, USF Health College of Pharmacy

Committee member for Admissions (College of Pharmacy)

Curriculum Committee member (College of Pharmacy)

Executive committee member for White coat ceremony

Faculty Council: USF Health College of Pharmacy

Awards and Honors:

American Heart Association, Great Rivers Affiliate- Beginning grant-in-aid

American Heart Association, Ohio Valley Affiliate-Post-doctoral fellowship

Visiting Associate Professor, Sullivan University-College of Pharmacy, Louisville, KY

Senior Research Fellowship, CSIR, New Delhi, India.

Satu Somani Junior Scientist Award in Physiology 2004, Association of Scientists of

Indian Origin in America (ASIOA)

Best Poster Presentation award for Scientific importance at Research Louisville -2009

(Presenter)

Professional Memberships:

2002- present Member, American Heart Aassociation, Basic Cardiovascular Sciences

2009-present, Diabetes and Obesity Center, University of Lousiville

2011-present, American Association for College of Pharmacy

Publications.

1. Kolliputi N, Galam L, Parthasarathy PT, Tipparaju SM, Lockey R. NALP3

Inflammasome Silencing Attenuates Ceramide Induced Transepithelial Permeability,

Accepted 2012 (in press) Journal of Cellular Physiology.

2. Barski OA, Tipparaju SM, Bhatnagar A. Kinetics of nucleotide binding to the beta -

subunit (AKR6A2) of the voltage-gated potassium (Kv) channel. Chem Biol Interact.

2009, 178, 165-70.

3. Barski OA, Tipparaju SM, Bhatnagar A. The aldo-keto reductase superfamily and its

role in drug metabolism and detoxification. Drug Metab Rev. 2008, 40, 553 -624.

(Review)

4. Tipparaju SM, Barski O, Srivastava S, Bhatnagar A. Catalytic mechanism and

substrate specificity of the -subunit of the voltage-gated potassium channel.

Biochemistry. 2008 47, 8840-54.

5. Moshal KS, Tipparaju SM, Vacek TP, Kumar M, Singh M, Frank IE, Patibandla PK,

Tyagi N, Rai J, Metreveli N, Rodriguez WE, Tseng MT, Tyagi SC. Mitochondrial matrix

metalloproteinase activation decreases myocyte contractility in

hyperhomocysteinemia. Am J Physiol Heart Circ Physiol. 2008 295, H890 -7.

6. Tipparaju SM, Liu SQ, Barski OA, Bhatnagar A. NADPH binding to -subunit regulates

inactivation of voltage-gated K+ channels. Biochem. Biophys. Res. Commun. 2007, 359,

269-76.

7. Tipparaju SM, Saxena N, Liu SQ, Kumar R, Bhatnagar A. Differential regulation of

voltage-gated K+ channels by oxidized and reduced pyridine nucleotide coenzymes.

Am. J Physiol. Cell Physiol. 2005, 288, C366-76.

8. Wagner MB, Wang Y, Kumar R, Tipparaju SM, Joyner RW. Calcium transients in

infant human atrial myocytes. Pediatric Res. 2005, 57, 28-34.

9. Tipparaju SM, Kumar R, Wang Y, Joyner RW, Wagner MB. Developmental

differences in L-type calcium current of human atrial myocytes. Am. J Physiol. Heart

Circ Physiol. 2004, 286, H1963-9.

10. Wang Y, Xu H, Kumar R, Tipparaju SM, Wagner MB, Joyner RW. Differences in

transient outward current properties between neonatal and adult human atrial

myocytes. J Mol. Cell. Cardiol. 2003, 35, 1083-92.

11. Bhatnagar A, Kumar R, Tipparaju SM, Liu SQ. Differential pyridine nucleotide

coenzyme binding to the -subunit of the voltage-sensitive K+ channel: a mechanism

for redox regulation of excitability? Chem. Biol. Interact. 2003, 143-144, 613-20.

12. Tipparaju SM, Kilfoil, PJ, Li X, Bhatnagar A, Barski O. Cytosolic C-terminal domain of

Kv1.5 senses pyridine nucleotide binding of the -subunit. Manuscript under

preparation. (Submitted to Pfl gers Archiv: European Journal of Physiology, accepted)

13. Tipparaju SM, Bhatnagar A, Barski O. Tetrameric structure allows regulation of the

rate and the extent of inactivation of Kv channels by pyridine nucleotides. ( Manuscript

under preparation).

14. Kilfoil, PJ, Tipparaju SM, Barski O, Bhatnagar A. Regulation of Ion channels by

Pyridine nucleotides. Invited Review by Circulation Research (Under Preparation).

Book Chapters:

1. Tipparaju, S.M., and Bhatnagar, A. Pipette Perfusion: Methods and Application. Patch-

Clamp Analysis: Advanced Techniques. Vol. 38, Page 309 -324. Editors: Wolfgang W., Boulton,

A.A., and Baker, G.B. Humana Press, Totowa, New Jersey 2006.

2. Tipparaju, S.M., Bhatnagar, A., and Barski O.A. Catalytic activity of the -subunits of the

voltage-sensitive potassium channels and its regulation by pyridine nucleotides. Page 401 -410.

Enzymology and molecular biology of carbonyl metabolism 13, Editor: Weiner, H. Purdue

University Press, W est Lafayette, Indiana 2006.

Abstracts:

1. Kilfoil PJ, Barski OA, Brittan K, Prabhu SD, Bhatnagar A, Tipparaju SM. Genetic

Ablation of Potassium Channel Subunit Kv 2 Slows Repolarization in the Cardiac

Septum and Leads to Cardiac Remodeling. (Presented at AHA Scientific Sessions-2011

Orlando, FL)

2. Tipparaju SM, Barski, OA, Bhatnagar, A. Gating mechanism and pyridine nucleotide

sensitivity of voltage-gated potassium channels with its -subunit: Role of Kv C-

terminus. Gordon Research Conference: Cardiac Arrhythmia Mechanisms, Barga

(Lucca), Italy, 2009.

3. Barski, OA, Li, XP, Brooks AC, Salabie JK, Bhatnagar A, Tipparaju SM. Redox

regulation of Kv channels: Obligatory role of Kv C-terminus in sensing pyridine

nucleotide binding to Kv subunits. AHA Scientific Sessions 2009, Orlando, FL.

4. Tipparaju SM, Barski, OA, Bhatnagar, A. Mechanism of enzymatic activity of -subunit

of voltage-gated potassium channels. Biophysical Society Meeting, Long Beach, CA,

2008.

5. Barski, OA, Tipparaju SM, Bhatnagar, A. Pyridine nucleotide dependent Kv inactivation

and mathematical modeling of subunits. Biophysical Society Meeting, Long Beach, CA,

2008.

6. Tipparaju SM, Vila, E., Bhatnagar, A, and Barski, OA. Role of Nucleotide Binding and

Catalysis in Regulating -Subunit Mediated Inactivation of Voltage-Gated K+ channels.

Circulation; Abs, 2006.

7. Tipparaju SM, Liu SQ, Barski OA, Bhatnagar A. Redox regulation of voltage -gated

potassium channel Kv1.5 by -subunits: Differential effects of Kv 1.3 and 2.1.

Circulation;112, supplement II 17 (Abs. #548) 2005.

8. Tipparaju, SM, Kumar R, Liu SQ, Bhatnagar A. Pyridine coenzyme binding to Kv-

proteins imparts redox-sensitivity to Kv channels. Circulation; 110, supplement III 62 (Abs.

# 286) 2004.

9. Kumar R, Liu SQ, Tipparaju, S.M., Bhatnagar A. Regulation of Kv channel activity by

differential pyridine nucleotide binding to -subunits. North American Society for Pacing

and Electrophysiology (NASPE) meeting: Washington D.C., May 2003.

10. Tipparaju SM, Liu SQ, Bhatnagar A, Kumar R. Protein Kinase C dependent

phosphorylation regulates Kv 1.5 channel function. Experimental Biology 2003, San

Diego CA.

11. Bhatnagar A, Tipparaju SM, Liu SQ, Kumar R. Pyridine nucleotide coenzymes regulate

inactivation of voltage sensitive potassium channels by -subunits. Circulation,

supplement II, 106, 19 II-47 (Abs. # 233) 2002.

12. Tipparaju SM, Jain SK, Dubey MP, Ray M. Evaluation of ischemia-reperfusion injury in

hypertrophic rat hearts Functional and cellular level alterations. Indian J Pharmacol; 33,

301, (Abs.# 209), 2001.

13. Tipparaju SM, Jharna A Dubey MP. Endothelial cells as a target for altered vascular

response in pressure overload cardiac hypertrophy. Indian J Pharmacol; 32: 80 (Abs. #

161), 2000.

14. Tipparaju SM, Dubey MP, Jharna A, Ray M. Studies on various interventions related to

hypertrophic rat heart preparation. Indian J Pharmacol; 31: 46 (Abs. # 27), 1999.

15. Ray M, Chandra R, Srinivas T, Dubey MP. Role of nitric oxide and free oxygen radicals in

ischemia reoxygenation in aortas from hypertensive rats. Indian J Pharmacol; 31: 46 (Abs.

# 25), 1999.

Journal Reviewer

Cardiovascular Toxicology

Toxicology and Applied Pharmacology

PLOS one

Academic Services:

1. Supervise Graduate Students: Trained and supervised Ph.D and Master level students at

University of Louisville. Identify and troubleshoot project related problems Coordinate with

other faculty members and develop successful approaches.

2. Presented Work-in-progress Seminar Series talk at Department of Molecular Medicine,

USF Health- Morsani College of Medicine, Graduate Seminar GMS 7939. The talk wa s

entitled Redox regulation of Kv channels: role of Kv subunits in physiology and disease.

3. Cardiology Grand rounds: Presentation at the annual cardiology grand rounds at Jewish

Hospital and University of Louisville, Louisville, KY.

4. Invited Presentation at Drug Discovery Symposium in Post-Genomic Era, June 2011

USF-College of Pharmacy

5. Oral Presentations at American Heart Association-Scientific Sessions 2004, 2006

6. PhD Thesis reviewer: Phytochemical and Pharmacological evaluation of Plant Leucas

Aspera Spreng . Vinayak Mission University, India

Teaching:

Courses Years Number of Students University

lectures/Sessions

Pharmacokinetics and 2008-2009 2 Pharm D Sullivan University-

Drug Metabolism (PY2) College of

Pharmacy

Drug Delivery Systems-I 2011 7 Pharm D USF College of

(Class) (PY1) Pharmacy

Drug Delivery Systems-I 2011 13 Pharm D USF College of

(Laboratory) (PY1) Pharmacy

Drug Delivery Systems-II 2012 3 Pharm D USF College of

(Class and Laboratory) (PY1) Pharmacy

Research Personnel Trained:

Graduate Students, laboratory personnel

Ermin Vila 2007-2008 MS (Biochemistry) University of Louisville

Alan Brooks 2009-2010 MD, PhD University of Louisville

Peter Kilfoil 2010-2011 PhD University of Louisville

Jayesh Thanki 2011 MS (Biotechnology) USF Tampa

Ana Groshev 2011-2012 BS (Honors College) USF Tampa

Katie Karch 2011-2012 BS USF Tampa

Siva Panguluri 2012- Research Associate USF Tampa

Research Support.

Ongoing Projects:

NIH R01HL-102171 Tipparaju (PI) 5 years, Tipparaju (PI) 250,000/year

Title: Redox regulation of Kv currents

The aim of this project is to understand the pyridine nucleotide dependent regulation of Kv

currents in the heart.

University of Louisville Pilot grant Barski (PI), Tipparaju (Co-PI) 50,000

Title: Drug Development for preventing Kv hyperexcitability

Aim of this project is to identify ligands that bind Kv subunits and use them as drug targets

for hyperexcitability disorders.

Pending:

BASF Pathak (PI), Tipparaju (Co-PI) 252,000

Proposal for establishing: Center for excipient Research at USF-College of Pharmacy

Aim: To perform independent research for pharmaceutical excipients. Provide training and

research experience to Pharm D students and graduate students for dosage form and drug

delivery related research.

Completed Projects:

0865466D AHA BGIA Great Rivers Affiliate Tipparaju (PI) 110,000

07/01/2008-06/30/2010

Regulation of voltage-gated potassium channel by Kv in the heart

The aim of this project is to investigate the role of Kv subunits for metabolic regulation

and oxygen-sensing in the heart

HL-089372 NIH R21 Tipparaju (Co-PI), Barski (PI) 250,000

02/01/2008-02/01/2010 (Under no cost extension)

Oxidoreductase activity of the -subunit of the Kv channels

In this project we aim to identify the physiological substrate for Kv -subunits and to

investigate if catalytic activity of Kv -subunit can lead to functional alterations in Kv currents.

0425439B AHA post-doctoral fellowship Tipparaju $82,000

07/01/04-06/30/06

AHA-Ohio Valley Affiliate

Redox regulation of Voltage-sensitive potassium channels

The aim of this project is to examine how pyridine coenzymes regulate the Kv currents

and whether the binding of the coenzymes to Kv -subunit changes the gating properties

of Kv currents.

Senior Research Fellow: Pre-doctoral fellowship Tipparaju

04/28/1998- 09/0/2001

Role of Nitric oxide in pressure-overload cardiac hypertrophy

Aim of the pre-doctoral dissertation work was to determine the differential effects of nitric

oxide pathway mediators on hypertrophic rat hearts

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