Name: KEVIN R. NASH

Address: *** * ********* **

Tampa, FL 33604

Cell Phone: 352-***-****

Email: abpepk@r.postjobfree.com

Immigration status: Permanent Resident: Green card

Positions

Assistant Professor University of South Florida, Mol. 2008-

Pharm. & Physiol. present

Research Assistant Professor Powell Centre for Gene Therapy, 2002-2008

University of Florida, Mol. Gen. & Micro.

Advisor Dr. N. Muzyczka

University of Florida, Mol. Gen. &

Post-doctoral associate 2000-2002

Micro.

Advisor Dr. N. Muzyczka

Field of study: AAV in vitro DNA

replication, and AAV gene therapy.

University of Florida, Biochemistry

Post-doctoral associate 1997-1999

Dept.

Advisor Dr. B. Dunn

Field of Study: HIV and Hepatitis C

protease kinetics and inhibitor design

and testing.

Education and Training

Ph.D. in Biochemistry University of Queensland, Biochemistry 1993-1996

Dept.,

Title: "Structure function studies on

purple acid phosphatase."

Tutoring assistant University of Queensland, Biochemistry 1992-1996

Dept. (Teaching Biochemistry Practical.)

Kevin Nash pg. 1

First Class Honors University of Queensland. 1992

Biochemistry Title: "Development of non-radioactive

assays for protein phosphotyrosine

phosphatases."

Studentship Studentship at Commonwealth 1991

Scientific and Industrial Research

Organization

Bachelor of Science University of Queensland. Majoring in 1989-1991

Biochemistry and Microbiology.

Publications

Nash, K.; M. Feldmuller, J. de Jersey, P. Alewood and S. Hamilton (1993)

Continuous And Discontinuous Assays For Phosphotyrosyl Protein Phosphatases

Using Phosphotyrosyl Peptide Substrates. Analytical Biochemistry, 213: 303-309.

PMID: 8238906

Marshall, K.; K. Nash, G. Hausmann, I. Cassady, D. Hume, J. de Jersey and S.

Hamilton (1997).

Recombinant Human and Mouse Purple Acid Phosphatases: Expression and

Characterization. Archives of Biochemistry & Biophysics, 345(2): 230-236. PMID:

9308894

Dunn, B. M.; M. W. Pennington, D. C. Frase and K. Nash (1999).

Comparison of Inhibitor binding to FIV and HIV Proteases: Structure-Based Drug

Design and the Resistance Problem. Biopolymers, 51(1): 69-77 PMID: 10380354

Valizadeh M, Schenk G, Nash K, Oddie GW, Guddat LW, Hume DA, de Jersey J,

Burke TR Jr, Hamilton S. (2004) Phosphotyrosyl peptides and analogues as

substrates and inhibitors of purple acid phosphatases. Arch Biochem Biophys. Apr

15;424(2):154-62. PMID: 15047187

Burger, C.; K. Nash and R. J. Mandel. (2005) Recombinant Adeno-Associated Viral

Vectors in the Nervous System. Hum Gene Ther. Jul;16(7):781-91. PMID:

16000060

Rodriguez, E.; E. M. Denovan-Wright, K. Nash, A. S. Lewin and R. J. Mandel. (2005)

Intrastriatal rAAV-mediated delivery of anti-huntingtin shRNAs induces partial

reversal of disease progression in R6/1 Huntington s disease transgenic mice. Mol.

Ther. 12: 618-3. PMCID: PMC2656966

Kohlbrenner, E.; G. Aslanidi, K. Nash, S. Shklyaev, M. Campbell-Thompson, B. J.

Byrne, R. O. Snyder, N. Muzyczka, K. Warrington, S. Zolotukhin. (2005)

Successful Production Of Pseudotyped rAAV Vectors Using A Modified Baculovirus

Expression System. Mol. Ther. 12: 1217-25. PMCID: PMC1351154

Mandel R.J.; Manfredsson F.P., Foust K.D., Rising A., Reimsnider S., Nash K., Burger

C. (2006) Recombinant adeno-associated viral vectors as therapeutic agents to treat

neurological disorders. Mol Ther. 13(3):463-83. PMID: 16412695

Nash, K.; W.J. Chen, W.F. Mc Donald, X. Zhou and N. Muzyczka. (2007) Purification

Kevin Nash pg. 2

of Host Cell Enzymes Involved in Adeno-Associated Virus DNA Replication. J.

Virology. 81(11): 5777-5787. PMCID: PMC1900299

Nash K., W. Chen, and N. Muzyczka (2008) Complete in vitro reconstitution of AAV DNA

replication requires the MCM complex. Journal of Virology 82: 1458-1464. PMCID:

PMC2224442

Carty N., Nash K., Lee D., Mercer M., Gottschall P.E., Meyers C., Muzyczka N.,

Gordon M.N., Morgan D. (2008) Adeno-Associated Viral Vector Mediated Gene

Delivery of Endothelin Converting Enzyme Reduces A Deposits in APP/PS-1

Transgenic Mice. Molecular Therapy 16(9):1580-6. PMCID: PMC2706523

Nash K., W. Chen, M. Salganik and N. Muzyczka (2009) Identification of cellular

proteins that interact with the adeno-associated virus rep protein. J Virol. 83(1): 454-

69. PMCID: PMC2612328

Levy H.C., Bowman V.D., Govindasamy L., McKenna R., Nash K., Warrington K.,

Chen W., Muzyczka N., Yan X., Baker T.S., Agbandje-McKenna M. (2009) Heparin

binding induces conformational changes in Adeno-associated virus serotype 2. J

Struct Biol. 165(3):146-56. PMID: 19121398

Galleguillos D, Fuentealba JA, G mez LM, Saver M, G mez A, Nash K, Burger C,

Gysling K, Andr s ME. (2010) Nurr1 regulates RET expression in dopamine neurons

of adult rat midbrain. J. Neurochem. 114(4):1158-67. PMID: 20533997

Gorbatyuk OS, Li S, Nash K, Gorbatyuk M, Lewin AS, Sullivan LF, Mandel RJ, Chen

W, Meyers C, Manfredsson FP, Muzyczka N. (2010) In Vivo RNAi-Mediated alpha-

Synuclein Silencing Induces Nigrostriatal Degeneration. Mol. Ther. 18(8): 1450-7.

PMID: 20551914

Lebson L., K. Nash, S. Kamath, D. Herber, N. Carty, D. C. Lee, Q. Li, K. Szekeres, U.

Jinwal, J. Koren, C. A. Dickey, P. E. Gottschall,1 D. Morgan and M. N. Gordon

(2010) Trafficking CD11b-Positive Blood Cells Deliver Therapeutic Genes to the

Brain of Amyloid-Depositing Transgenic Mice. J. Neurosci. 30(29): 9651 9658

PMID: 20660248

Carty N., D. Lee, C. Dickey, C. Ceballos-Diaz, K. Jansen-West, T.E. Golde, M.N.

Gordon, D. Morgan, K. Nash (2010) Convection-Enhanced Delivery And Systemic

Mannitol Increase Gene Product Distribution Of AAV Vectors 5, 8, And 9 And

Increase Gene Product In The Adult Mouse Brain. J. Neurosci. Methods. 194(1):

144-53.

Choi, Y-K., K. Nash, B.J. Byrne, N. Muzyczka and S. Song. (2010) The effect of DNA-

dependent protein kinase on adeno-associated virus replication. PLoS ONE. 20(5):

e15073.

Daily, J., K. Nash, U. Jinwal, T. Golde, J. Rogers, M. M. Peters, R. Burdine, C Dickey,

J Banko and E. J. Weeber. (2011) Adenovirus-mediated rescue of the cognitive

defects in a mouse model for Angelman Syndrome. PLoS ONE. 6 (12): e27221

Li Q, Nash K, Shah B, Lebson L, Grimm J, Rosenthal A, Selenica M-L, Morgan D,

Gordon MN. (2012) Chronological Age Impacts Immunotherapy and Monocyte

Kevin Nash pg. 3

Uptake Independent of Amyloid Load. J Neuroimmune Pharmacol. 7(1):202-

14..

Nash K.R., M. C. Cardenas-Aguayo, M. J. Berg, and N. Marks Transduction Of E13

Murine Neural Precursor Cells (NPCs) By Non-Immunogenic rAAVs Induces

Major Changes In Neuronal Phenotype. J. Neurosci. Accepted

Funding.

Current Funding Support

PI: MJ Fox Foundation. Co-PI Paula Bickford. Modulation of Microglial Activation.

Funding $250K 06/11 to 05/13.

PI: USF. Neuronal Progenitor Cell Differentiation into Neuronal Cells with rAAV.

Funding $20K 07/11 to 06/12

Submitted Funding Support

PI: Alzheimer s Assoc. Fractalkine and Neuropathology in AD Transgenic Mice.

Proposed Funding $240K

Completed Funding Support:

Co-I: NIH R01. AAV Gene Therapy for Alzheimer's Disease. Funding $1.5 mil. 2005-

2010

PI: Florida ADRC. Funding $30K. Gene Therapy for Alzheimer's Disease Using

Recombinant Adeno-Associated Virus. 2009-2010.

Research And Management Experience

Director of USF Viral Vector Core.

Assist or direct the design and production of viral vectors for faculty at USF.

Management of of Post-doctorates, lab technicians and graduate students and

directing experimental research focus.

Research Assistant Professor/Post-doctorate Mol. Gen. & Micro. Dept. U.F.

Identification of host cell factors that are involved in the replication of Adeno-

associated virus (AAV). This has involved extensive cell fractionation and protein

purification as well as recombinant protein work to identify the essential proteins

required for virus replication.

Design of AAV gene therapy vectors for the study and treatment of Alzheimer s

disease. To this end we have produced recombinant AAV, that in preliminary

studies, reduces the -amyloid load on Alzheimer model mice. We are also

examining the use of AAV with shRNA to reduce the levels of BACE protein in the

same animal models.

Management of Post-doctorates, lab technicians and graduate students and

Kevin Nash pg. 4

directing experimental research focus.

Post-doctorate Biochemistry UF

Study of Inhibitors and mutants of HIV, FIV and HCV proteases. Kinetic analysis

peptide analog inhibitors using spectrophometric assays.

Management of Lab technicians and graduate students and directing experimental

research.

Ph.D. University of Queensland

Expression and purification of native and recombinant baculovirus

phosphotyrosine phosphatases for characterization and potential significance in

osteoporosis.

Teaching of practical courses to undergradate students, e.g. HPLC techinques and

uses; molecular biology techniques; and protein purification.

Professional Associations/Affiliations

ASNTR Education and Training Committee

Society for Neuroscience

ISTART, Alzheimer Association

References

Dr. Dave Morgan Dr. Corinna Burger

University of South Florida University of Wisconsin

Byrd Alzheimer Institute Department of Neurology

4001 E Fletcher Ave Medical Sciences Center

MDC Box 36 1300 University Av

Tampa FL 33612-4799 Rm 73 Bardeen

Ph: +1-813-***-**** Madison WI 53706

Ph: 608-***-****

Dr. Nicholas Muzyczka

University of Florida

Department of Molecular Genetics and

Microbiology

Powel Gene Therapy Center

1376 Mowry Rd

Gainesville, FL 32610

Ph: 352-***-****

Kevin Nash pg. 5

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