Quality Assurance Control

Timothy Miller

Email: abpehf@r.postjobfree.com

Address: **** ******* **.

City: Coatesville

State: PA

Zip: 19320

Country: USA

Phone: 610-***-****

Skill Level: Management

Salary Range: $35,000

Willing to Relocate

Primary Skills/Experience:

See Resume

Educational Background:

See Resume

Job History / Details:

TIMOTHY J. MILLER

1704 Valmont Drive

Coatesville, PA 19320

610-***-**** abpehf@r.postjobfree.com

SUMMARY

Biomedical researcher with extensive experience in gene therapy research, vaccine production, protein expression / purification and neuroscience. Fluent in English and German.

* Assay development * Immunohistochemistry

* Bacterial and molecular cloning * Mammalian cell culture

PROFESSIONAL EXPERIENCE

ROCK THE SPA, LLC 2010 - 2012

Director of Research

Oversaw all projects, initiatives and research involving the development of a revolutionary new polymer-based massage stone that is inductively charged and retains heat for seven hours. Involved with all aspects of QA and QC testing: testing the durability and water-proofing of the outer shell of the product, the integrity and functionality of the interior electronic components, and all testing that was necessary for UL safety certification.

CHILDREN`S HOSPITAL OF PHILADELPHIA, Philadelphia, PA 2006 - 2010

Vector Production Manager / Phillip Johnson Laboratory

Produced and purified recombinant AAV viral vectors by FPLC (using the AKTA Explorer system powered by UNICORN software) for use as experimental vaccines.

* Titered purified virus using the ABI FAST Real-Time PCR System.

* Cloned and purified vector plasmids used for calcium chloride transfection.

* Maintained cell lines used for vector production.

* Supervised and trained junior staff members.

* Performed animal studies in rabbits to evaluate the safety and efficacy of vectors as

potential vaccines that complied with Investigational New Drug submission guidelines.

UNIVERSITY OF PENNSYLVANIA SCHOOL OF MEDICINE 1997 - 2005

Research Specialist / Department of Anesthesia (2003 - 2005)

* Characterized the proteome of a novel glutamate receptor-associated transcription unit, GRINL-1A, by (1) antibody inhibition of NMDA toxicity (measured by MTT and LDH assays) in cultured cortical and hippocampal neurons, (2) Western blots and (3) immunofluorescent staining (http://www.ncbi.nlm.nih.gov/pubmed/15233991).

* Prepared primary cultures of cortical neurons from fetal rats.

* Used bacterial cloning to express and purifiy a peptide containing the putative anesthetic binding site of the nicotinic acetylcholine receptor alpha-M2 subunit.

* Evaluated isothermal calorimetry data to determine the binding kinetics of halothane and human serum albumin.

TIMOTHY J. MILLER

215-***-**** Page 2 abpehf@r.postjobfree.com

PROFESSIONAL EXPERIENCE (Continued)

Research Specialist / Department of Neurology, Neuro-oncology Division (2002 - 2003)

* Performed immunoblots of patient sera and CSF against proteins from cultured neuronal cells that

express paraneoplastic antigens (http://onlinelibrary.wiley.com/doi/10.1111/j.1-750-***-****.tb00226.x/abstract).

* Performed immunohistochemistry of frozen rat brain sections using patient sera.

* Screened cerebellar and brainstem cDNA library using phage display.

* Performed molecular cloning, PCR, immunofluorescent staining, immunoprecipitation, and Western blots.

Research Specialist / Institute for Human Gene Therapy (1997 - 2002)

* Produced and purified recombinant adenovirus and AAV vectors in compliance with GLP/GMP standards.

* Cloned plasmids for production of viral vectors by transfection of mammalian cell lines.

* Generated an AAV-producing cell line that was used to produce AAV without transfection (only super-infection with adenovirus was required).

* Analyzed transgene expression by Western/Southern blot, ELISA, FACS and PCR.

* Performed immunological assays to detect pre-existing neutralizing antibodies to AAV vectors.

* Performed quality control / quality assurance assays and documentation for titer of infectious viral particles, presence of replication competent adenovirus and endotoxin.

* Performed PCR genotyping and maintained breeding records for transgenic mice which were used to study the role of beta-defensin 1 in pulmonary innate immunity: (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC127957/).

DIVERSA CORPORATION, Sharon Hill, PA 1996 - 1997

Research Assistant

* Subcloned novel microbial genes for expression of potential thermostable enzymes.

* Purified and lyophilized affinity tagged proteins.

* Sequenced DNA libraries from thermophilic eubacteria and archaebacteria.

* Analyzed and edited sequence data.

EDUCATION

B.A., Biology, University of Pennsylvania

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