Maintenance School

vera alexeeva

Email: *********@********.***

Address: *** ****** *** ***

City: new York

State: NY

Zip: 10010

Country: USA

Phone: 917-***-****

Skill Level: Experienced

Salary Range: $65,000

Primary Skills/Experience:

See Resume

Educational Background:

See Resume

Job History / Details:

Vera Alexeeva, Ph. D.

Mount Sinai School of Medicine, New York

New York, NY, 10029

e-mail ****.********@****.***, tel.917-***-****

Summary

Research scientist position in molecular and cellular biology, biochemistry, molecular biology

Research Experience

2009-present Assistant Scientist- Black Family Stem Cell Institute Mount Sinai School of Medicine New York, NY

* Work at Human Embryonic Stem Cell Core Facility. Participate in generation of mouse

and human induced pluripotent stem cells, maintenance and differentiation of embryonic stem cell lines. Generated Human and Mouse iPS cells using retroviral, lentiviral, and PiggiBac systems. Created a stable lines of hESC for fluorescent tracking and drug resistance selection. Genetic engineering of human ES and iPS cells using ZFN and TALE nucleases.

Participate in teaching embryonic stem cells techniques students and researchers

2001-2009 Instructor- Department of Neuroscience, Mount Sinai School of Medicine, New York, NY

* Participated in a multidisciplinary research project aimed at understanding the neural basis of behavioral plasticity using molecular biology and biochemistry techniques.

Cloned and over-expressed of a variety of genes encoding different neuropeptides and transporters in the nervous system of sea slug Aplysia Californica. Over-expressed and purified several vesicular transporters for functional studies and antibody production. Developed in vitro assays and characterized activity of the purified proteins .

1995- 2001 Research Associate, Department Physiology&Biophysics, Mount

Sinai School of Medicine. New York, NY 10029

* Purified neuropeptides and transmitters from nervous tissue of Aplysia Californica using liquid chromatography.

Education

Ph.D. Chemistry, St. Petersburg Technological Institute, Russia.

M.S. Biology, St. Petersburg Technological Institute, Russia.

Citizenship USA

Expertise

* Cell culture maintenance; transfection, infection

* Culturing human and mouse embryonic stem cells.

* Generation Induced pluripotent cells, differentiation.

* FACS analysis.

* Immunostaining of cultured cells, fluorescence microscopy

* DNA, RNA and protein isolation.

* Real Time PCR, RT-PCR.

* Northern, Western, Southern blotting

* Cloning of PCR and plasmid fragments. Primers design. Cloning strategies including c DNA library screening, RT-PCR methods, RNA subtraction, 51 and 31 RACE, degenerate primer PCR. TALEN engineering. TruSeq RNA sample preparation (ILLUMINA)

* Affinity purification of expressed recombinant proteins Standard chromatography resins were used for recombinant protein purification: Q-and S-sepharose (Pharmacia), NiNTA agarose

* Assays of enzymatic activity (Choline Acetyltransferase activity, Glutamate and Acetylcholine transport in mammalian cells).

* Protein Expression. Used of E.coli expression systems and wide range of expression vectors for protein expression for antibody production and functional studies.

* In Situ Hybridization. Whole mount In Situ Hybridization method (ISH, FISH) was used for localization of expression of mRNAs encoding neuropeptides, hormones and neurotransmitter transporters in the nervous system of Aplysia Californica.

* Radioimmunoassay. Quantification the release of neuropeptides under physiological conditions. Iodination of small peptides and their purification using HPLC.

RECENT PUBLICATIONS (total 16)

1.Vilim FS, Sasaki K, Rybak J, Alexeeva V, Cropper EC, Jing J, Orekhova IV, Brezina V, Price D, Romanova EV, Rubakhin SS, Hatcher N, Sweedler JV, Weiss KR.J Neurosci. 30:131-47 (2010)

Distinct mechanisms produce functionally complementary actions of neuropeptides that are structurally related but derived from different precursors.

2. Jing J, Sweedler JV, Cropper EC, Alexeeva V, Park JH, Romanova EV, Xie F, Dembrow NC, Ludwar BC, Weiss KR, Vilim FS. J. Neurosci. Dec8; 30(49): 16545-58 (2010).

Feedforward compensation mediated by the central and peripheral actions of a single neuropeptide discovered using representational difference analysis.

3. Jing J, Vilim FS, Horn CC, Alexeeva V, Hatcher NG, Sasaki K, Yashina I, Zhurov Y, Kupfermann I, Sweedler JV, Weiss KR. J Neurosci. 27: 3490-502 (2007).

From hunger to satiety: reconfiguration of a feeding network by Aplysia neuropeptide Y.

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