Training San Francisco

SREENIVASAN PARUTHIYIL

Email: *********@********.***

Address: **** ******* ******

City: San Francisco

State: CA

Zip: 94122

Country: USA

Phone: 415-***-****

Skill Level: Experienced

Salary Range: $90,000

Primary Skills/Experience:

See Resume

Educational Background:

See Resume

Job History / Details:

SREENIVASAN PARUTHIYIL

Sreenivasan Paruthiyil Phone: 415-***-**** (H)

1825 Noriega Street, Apt#310 415-***-****(cell)

San Francisco

CA -94122 mail: *********@********.***

PROFILE

PhD Level Biologist with ten years of postdoctoral research and four years of industry experience in cell and animal models of breast cancer. Enrolled in Certificate program in Clinical Trials Design and Management at UCSC Extension. Completed a course on GCP including FDA and ICH guidelines.

aa Researcher with training in broad areas of biology-oncology, immunology, endocrinology

aa Experience in signaling pathways regulating cell proliferation and cell cycle, transcriptional regulation of oncogenes and tumor suppressor genes. Xenograft and mouse models of cancer.

aa Technical skills in molecular biology, adenoviral and lentiviral vectors, cell culture

aa Training in mammalian reproductive physiology, animal surgery, transgenic rat generation

aa Strong publication record in major journals. Member of professional associations.

aa Productive independent researcher with communication, team skills& training experience(students and SRAs)

TECHNICAL SKILLS

Animal models: Tumor model: Conducting studies in tumor progression using xenograft models in nude mice. Drug/adenoviral delivery using osmotic pumps and study the tumor progression in implantation sites such as subcutaneous and mammary fat pad. Transgenic rat: Generation and characterization of transgenic rat. Perform In vivo studies in conscious rats, serial blood collection from indwelling catheters and surgical manipulations. Cell culture: experience with various cell types, isolation of primary cells from mouse mammary gland, study of cellular immunity, cell cycle DNA analysis (FACS), cell proliferation, transfection, perifusion of cells to study hormone release. Molecular Biology: General molecular biology procedures including PCR, cloning, subcloning and western blot. Functional genomics: Preparation and use of adenoviral and lentiviral vectors for use in invitro and invivo models, preparation of lentiviral vectors for knock-down of genes. Techniques in gene expression analysis: Real-time PCR, ChIP assay to study recruitment of proteins to the promoters, study of methylation of promoters to monitor promoter modification. Signal transduction pathway Analysis: ELISA techniques for enzymes (MAP kinase, Ras, cdc2 kinase) and cytokines, biochemical assays for enzymes (phosphodiestearases), radioimmunoassay for hormones and second messengers (LH, FSH, GnRH and cAMP)

PROFESSIONAL EXPERIENCE

Bionovo Inc. Research Scientist (2008-2012)

Involved in UC Berkeley and Bionovo collaborative development of hormone receptor mediated cancer therapeutics. Study of estrogen receptor beta mediated induction of tumor suppressor genes. Development of in-vivo tumor models to study tumor progression. Study of interactions of EGF/heregulin and estrogen receptor beta mediated signaling pathways in cell proliferation and mammary gland development.

University of California at San Francisco (UCSF) Post Graduate Researcher (2000-2007)

Functional Characterization of Estrogen Receptor beta in Breast Cancer Progression

Demonstrated that in estrogen receptor positive (ER-alpha positive) MCF7 cells, expression of ER-beta causes inhibition of cell cycle progression and tumor growth in mouse model. The drug selectivity of ER-beta in activation/repression of gene expression and the interaction of ER-beta with tamoxifen function were investigated in invitro systems. Further, the molecular mechanisms of ER-beta mediated induction of tumor suppressor genes by promoter regulation were elucidated using ChIP assays and protein kinase (cdc2) assays. Principal Investigator: Dr.Dale Leitman

University of California San Francisco(UCSF) Post Doctoral Fellow (1997-2000)

Transgenic Rat model to study GnRH release

Study of the involvement of cAMP mediated signaling pathway in GnRH release from hypothalamic neurons. Targeted expression of phosphodiesterase (PDE4D1) in GnRH secreting neurons of the hypothalamus lowered cAMP levels in these neurons. Serial blood sampling from jugular vein in ovariectomized rats showed significant reduction in the frequency of GnRH pulses. This study confirmed the importance of the cAMP signaling pathways in timing the pulsatile release of GnRH. During this work, I received extensive training in generation and characterization of transgenic rat model. Principal Investigator: Prof. Richard Weiner

All India Institute of Medical Sciences, New Delhi, India Post Doctoral Fello (1995- 1997)

T helper subsets and disease progression This study characterized the T helper subsets (Th1 and Th2) in an acute form of leprosy infection, namely, the Lepromatous Nodosum Leprosum (ENL) Type1 and 2 Reactions in leprosy patients. The study showed the change in cytokine profile from immunoprotective Th1 to anergic Th2 cytokines during the progression of the disease and the correlation with the clinical symptoms. Received training in Cellular and Molecular Immunology and conducting clinical research. Principal Investigator: Prof. Indira Nath

EDUCATION

Pondicherry University, Pondicherry, India.

Ph.D., Neuroendocrinology, February, 1989- January, 1995

Banaras Hindu University, Varanasi, India.

M.S., Zoology, 1986-1988

SELECTED PUBLICATIONS

(1) Paruthiyil S, Cvoro A Tagliaferri M, Cohen I, Shtivelman E, Leitman DC Estrogen receptor I causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2. Breast Cancer Res Treat 2011 Oct; 129(3):777-84

(2) Levy N, Paruthiyil S, Zhao X, Vivar OI, Saunier EF, Griffin C, Tagliaferri M, Cohen I, Speed TP, Leitman DC. Unliganded Estrogen Receptor-beta regulation of genes is inhibited by tamoxifen Mol Cell Endocrinol. 2010 Feb 5; 315(1-2)201-7

(3) Paruthiyil S, Cvoro A, Zhao X, Wu Z, Sui Y, Staub RE, Baggett S, Herber CB, Griffin C, Tagliaferri M, Harris HA, Cohen I, Bjeldanes LF, Speed TP, Schaufele F, Leitman DC

Drug and cell type-specific regulation of genes with different classes of estrogen receptor beta-specific agonists. PLoS One. 2009 Jul 17;4(7) :e6271

(4) Paruthiyil S., Parmar H, Kerekatte V, Cunha GR, Firestone GL, and Leitman DC Estrogen Receptor I Inhibits Human Breast cancer Cell Proliferation and tumor Formation by Causing a G2 cell Cycle Arrest. Cancer Research (2004) January 1, 64:423-428.

(5) Paruthiyil, S., Majdoubi M El, Conti, M. and Weiner, R I.

Phosphodiesterase expression targeted to gonadotropin-releasing hormone neurons inhibits

leutinizing hormone pulses in transgenic rats. Proc. Natl. Acad. Sci. USA (2002) 99 (26-171**-*****

(6) Sreenivasan Paruthiyil; MISRA, RS; WILFRED, D AND NATH, I.

Lepromatous leprosy patients show T helper-1 like cytokine profile with differential expression

of interleukin-10 during type 1 and 2 reactions. (1998) Immunol. 95(4): 529-536.

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