Development C

Kristina Schimmelpfeng Henthorn

**** *** ******** #***, ** JOLLA, CA 92037, T EL : 858-***-**** ********@*************.**

O bjective

Self motivated, goal oriented professional seeking a research position in a team of highly driven scientists.

Education

Ph.D. (Doctor of Natural Sciences) (2000) Westf lische Wilhelms-Universit t M nster, Germany

Master of Sciences (Diploma) (1996) Universit t zu K ln, Germany

Research and Technical S kills

M olecular Biology

DNA: Plasmid, cosmid and phage DNA preparations, subcloning and construct engineering, PCR, se-

quencing, Southern Blot, genomic and cDNA library screening, bioinformatics (comparative sequence

analysis)

RNA: mRNA puri cation, quantitative RT-PCR, whole mount in situ hybridization, RNAi

Histology & Imaging

Immunohistochemistry, immuno uorescence, uorescence and confocal microscopy, in vivo imaging

Biochemistry

PAGE, Western Blot, immunoprecipitation, luciferase assay

Cell Culture

Insect cell culture (Drosophila S2) and primary cultures of larval brain neurons

Genetics

Standard and advanced Drosophila genetics and husbandry, large scale EMS and P-element muta-

genesis, genetic mapping of mutations, generation of transgenic ies

Computing

Scienti c applications: NIH ImageJ, DNASTAR Lasergene, Olympus FV1000 operations software, Mo-

lecular Devices MetaMorph

General and Database Applications: Microsoft Word, Excel and PowerPoint, Adobe Photoshop, Illustra-

tor and Dreamweaver, Microsoft Access and FileMaker Pro

General Skills/Qualifications

I ndependently motivated, dedicated and passionate about biomedical research

E xcellent oral and written communication skills

F luent in German and basic knowledge of French

A bility to problem solve and effectively troubleshoot

K ristina Schimmelpfeng Henthorn Page 1

E xperience

Principal Scientist / Cell Biology

RGo Bioscience LLC, San Diego, California 2008-present

Developed cell based assays to study RNAi

Assistant Project Scientist

Department of Cellular and Molecular Medicine, University of California San Diego 2006-2008

Identi ed a new interaction partner of kinesin heavy chain in dendrites

Trained and supervised an undergraduate student to conduct a genetic screen and to phenotypically

characterize mutants using immuno uorescence and confocal imaging

Collaborated in a study aimed at uncovering functions of Amyloid Precursor Protein (APP) in axonal

transport and to identify possible transcriptional targets of APP using high-density oligonucleotide microar-

rays and RT-PCR

Collaborated in elucidating new functions for the dynein/dynactin complex in the nervous system

Postdoctoral Fellow

Department of Cellular and Molecular Medicine, University of California San Diego 2001-2006

Discovered a new function of kinesin heavy chain in axonal path nding and dendritic transport

Designed and executed a genetic screen to understand motor protein function in maintaining neuronal

polarity

Generated numerous transgenic ies carrying GFP-fused axonal and dendritic markers

Collaborated in a study to show the ability of kinesin-2 to differentially regulate the transport of two in-

dependent cargoes

Graduate Student

Institute for Neurobiology and Behavior, Westf lische Wilhelms-Universit t, M nster, Germany 1996-2000

Carried out a large scale EMS-mutagenesis and screened mutants for axon pattern defects

Established a model of commissure formation during Drosophila melanogaster embryonic development

Characterized and cloned a UDP-N-acetylglucosamine diphosphorylase involved in nervous system de-

velopment

Elucidated the role of two genes in a repulsive guidance system operating at the CNS midline

Taught Molecular Biology/Genetic/Developmental Biology laboratory class

Diploma Student

Institute for Developmental Biology, Universit t zu K ln, Germany 1995-1996

Performed a genome-wide screen in Drosophila melanogaster for axon pattern formation defects using

chromosomal de ciencies

Undergraduate Researcher

School of Biological Sciences, University of Sussex, Brighton, England 1994

Characterized the expression pattern of an enhancer-trap line in Drosophila melanogaster

K ristina Schimmelpfeng Henthorn P age 2

P ublications

Haghnia, M., Cavalli, V., Shah, S.B., Schimmelpfeng, K., Brusch, R., Yang, G., Herrera, C., Pilling, A. and

Goldstein, L.S.B. (2007) Dynactin is required for coordinated bidirectional motility, but not for dynein

membrane attachment. MBC; 18(6): 2081-89.

Schimmelpfeng, K., Strunk, M., Stork, T. and Kl mbt, C. (2006) mummy encodes an UDP-N-acetylglucosamine-

dipohosphorylase and is required during Drosophila dorsal closure and nervous system development.

Mech Dev; 123(6): 487-499

Baqri, R., Charan, R., Schimmelpfeng, K., Chavan, S., and Ray, K. (2006) Kinesin-2 differentially regulates the

anterograde axonal transport of acetylcholinesterase and choline acetyltransferase in Drosophila. J

Neurobiol. Mar; 66(4):378-92.

Stute, C., Schimmelpfeng, K., Renkawitz-Pohl, R., Palmer, R.H. and Holz, A. (2004) Myoblast determination in

the somatic and visceral mesoderm depends on Notch signalling as well as on milliways(mili(Alk)) as receptor

for Jeb signalling. Development, 131(4):743-54.

Schimmelpfeng, K., G gel, S. and Kl mbt, C. (2001) The function of leak and kuzbanian during growth cone

and cell migration. Mech Dev, 106, 25-36.

Hummel, T., Schimmelpfeng, K. and Kl mbt, C. (1999) Commissure formation in the embryonic CNS of

Drosophila: II. Function of the different midline cells. Development, 126, 771-779.

considered as equal first authors

Hummel, T., Schimmelpfeng K. and Kl mbt, C. (1999) Commissure formation in the embryonic CNS of

Drosophila: I. Identification of the required gene functions. Dev Biol. 1999 May 15;209(2), 381-98

Kl mbt, C., Schimmelpfeng K. and Hummel, T. (1997) Genetic analysis of axon pattern formation in the

embryonic CNS of Drosophila. Invertebrate Neuroscience, 3, 165-174.

Hummel, T., Schimmelpfeng, K. and Kl mbt, C. (1997) Fast and efficient egg collection and antibody staining

from large numbers of Drosophila strains. Development, Genes & Evolution, 207, 131-135.

Reviews

Kl mbt, C., Hummel, T., Granderath, S., Schimmelpfeng, K. (2001)

Glial cell development in Drosophila. Int J Dev Neurosci Jul;19(4):373-8. Review.

Kl mbt, C, Schimmelpfeng, K., Hummel, T. (1999) Glia development in the embryonic CNS of Drosophila.

Adv Exp Med Biol, 468, 23-32. Review.

Kristina Schimmelpfeng Henthorn P age 3

I nvited Oral Presentations

Lecture series: Molecular Analysis of Developmental Processes

Institute for Developmental Biology, Universit t zu K ln, Germany

2005

Smart Motors: The Role of Microtubule-Based Transport in Maintaining Neuronal Polarity

Biozentrum at the Universit t W rzburg, Germany 2005

Smart Motors: The Role of Microtubule-Based Transport in Maintaining Neuronal Polarity

SFB629 Seminar Series, Westf lische Wilhelms-Universit t M nster, Germany 2004

Smart Motors: The Role of Microtubule-Based Transport in Maintaining Neuronal Polarity

5th regional Drosophila conference in Marburg, Germany

1998

Genetic dissection of axon pattern formation in the embryonic CNS of Drosophila

Awards and Fellowships

Fellowship granted by the Deutsche Forschungsgemeinschaft (DFG) (SCHI 598/3-1) 2002-2004

Student Bursary to attend the 7th European Symposium on Drosophila Neurobiology in Warwick, UK 1998

Memberships/Activities

Member of the Association for Women in Science (AWIS) 2008

Af liate Member of the Genetics Society of America (GSA) 2002-2005

Postdoctoral Member of the American Society for Cell Biology (ASCB) 2001

Volunteer educator/guide in the Cologne Zoo 1991-1993

References available upon request

Kristina Schimmelpfeng Henthorn P age 4

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