Ben Alexander M.S.

M.B.A

Microbiology professional with 8 years of laboratory experience in the areas of

pharmacuticals, biofuels and genetic engineering.

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Curriculum Vitae

Skills Resume

Curriculum Vitae

Benjamin M. Alexander

1000C Cuerto Lane

Fort Collins, CO 80521

aborn6@r.postjobfree.com

970-***-****

Summary

Skilled research associate with over 6 years of experience in laboratory environment in

cell culture, molecular biology in mammals and plants and biochemistry. Experienced in

quality control, process development and research environments.

Education

M.B.A. Project Management Emphasis, College of Business,

Colorado State University, Ft. Collins, CO, Dec. 2011

M.S. Biochemistry, Department of Biochemistry,

Colorado State University, Ft. Collins, CO, 2003

B.S. Microbiology/Genetic Engineering, Department of Molecular, Cellular and

Developmental Biology,

University of California, Santa Barbara, CA, 1998.

Technical Skills

Biochemical Assays:

AlphaScreen assay, immunofluorescence, luciferase assay, ELISA, HTRF.

Functional Assays:

Luciferase reporter gene, Apoptosis, Signal transduction.

Cell Culture:

Extensive experience with cell culture and harvesting primary cells for culture using

laminin coated coverslips from adult and neonatal cardiomyocytes. Experience with cell

line passaging in flasks, electroporation of signal transduction constructs.

Plant Biology:

Transgenic plants and breeding for homozygous genotype, Algal aquaculture in flasks for

oil production.

Molecular Biology:

DNA purification, PCR, RNA extraction, RT-PCR, western blot, 2D gel electrophoresis,

chimera receptor construction.

HPLC:

Waters, Agelient, Reverse phase, Ion exchange.

Automation:

Tecan, Luciferase reporter gene, Apoptosis, HTRF.

In-Vivo:

Rat and mouse animal handling and dosing (IP, SC), euthanasia, surgery and dissection.

Computer/Equipment:

Microsoft office: Word, Excel, Powerpoint, Project, Outlook. Data analysis programs: SAS,

Softmax, StatLia, Electronic Notebook (Waters Vision), PD-LIMS (Thermo). Equipment: Vi-

Cell XR, Microbeta, Envision.Professional Experience

Amgen, Inc. (Aerotek) Boulder, CO. Feb 2011Jan 2012Research Associate I, Bioanalytical Science, Global Bioasay Group

Perform routine bioassays on drug products, manual and automated. Analyze data and input

results into LIMS and electronic notebooks in a process development lab environment

Passage and stage cells for use in bioassays

Participate in method and script qualification for new automated assays.

Maintain reagent stock

Process characterization of bioassays

Bioassay optimization

Redesign trending spreadsheets and assay worksheets to make them more efficient.

General lab maintenance

Colorado State University Fort Collins, CO. Aug 2002Aug 2009

Lab Technician/Research Associate (2008-2009

)

(

2008)Department of Chemical Engineering. Biofuels research with algae. Concerned with

screening strains for oil production and optimizing growth conditions. Using inexpensive

carbon sources to maximize algal growth and oil production. Method development for GC FID

analysis of oils from algae.

(

2009) Department of Biomedical Sciences. Performed research in obesity related heart

disease. Optimize cardiomyocyte cell isolation method. Wrote SOPs for cell culture

methods.

Graduate Research Associate (2002-2004), Department of Biology.

Performed research in a plant molecular biology laboratory.

Designed experiments to determine stability and functionality of transgenic signal

transduction circuit for the purpose of detecting biological weapons by changes of color

in the plant (DARPA funded).

Amplified and ligated cDNA to create a chimeric protein to create a ligand specific

receptor.

Created transgenic line of Arabidopsis thaliana with the chimeric protein

Elucidate nature and function of proteins that interact with a leucine-rich repeat

receptor-like kinase (Xa21.1). It is an analog of a gene in rice that is part of a

resistance pathway.

Graduate Teaching Associate (2004, 2008)

Taught varied sections of lab class for major and non-major students.

Created, administered and graded exams, assignments and quizzes.

Explained and aided students with experiments.

Tutored students from other sections and held review sessions.

Front Range Community College Fort Collins, CO. AugDec 2007Part-timeInstructor - Microbiology

Taught one section for pre-nursing students.

Developed and delivered lectures each class that made the material easy to understand.

Created, administered and graded exams, assignments and quizzes.

Explained and aided students with experiments.

Tutored students from other sections and held review sessions.

Lancaster Laboratories Longmont, CO. JulyOct 2006

Microbiologist, Quality Control.

Sampled RO, City, DI, Clean Steam and WFI from the manufacturing and support areas.

Tested samples for microbial growth; reported results by Laboratory Information

Management System (LIMS). cGMP trained. Resampled out of spec tests.

Microphage (Kelly Scientific

)

Longmont, CO. Nov 2005Mar 2006Research Associate, Research and Development.

Developed a rapid identification of bacteria using bacteriophages for clinical

laboratories.

Set up and performed experiments to look for bacteriophage infection that is species

specific.

Developed rapid detection method for clinical lab use, specifically E. coli and S.

aureus.

Maintained bacteria and phage stocks by passaging bacteria and harvesting phages grown on

host bacteria

Biosafety Level 2 environment.

Amgen, Inc. (Kforce) Longmont, CO. JulyNov 2005Research Associate, Process Development.

Characterization of products starting mass production.

Quantified and characterized product using reverse phase and ion exchange HPLC.

Prepared and ran samples on Waters and Agilent machines including buffers.

Sampled prep methods included enzymatic digestion, gravimetric dilutions and

concentration of samples by centrifugation. SDS-PAGE gel analysis of purification samples

by silver staining.

Analyzed and recorded results on excel spreadsheets and placed in notebooks and LIMS.

IDEC Pharmaceutical San Diego, CA. July 2001Aug 2002

QC Micro II, Quality Control

Water sampling and Environmental Monitoring (EM) in a cGMP environment.

Sampled and tested City, RO, Clean Steam and WFI in manufacturing and clinical for

microbial contamination.

Performed EM in manufacturing, clinical and the microbiology lab to ensure microbial

contaminations were minimal. Reported results to appropriate departments and recorded

results into LIMS and paper hard copies.

San Diego State University/Rees-Stealy Research Foundation San Diego, CA. Aug 1999May 2001Graduate Research Associate, Department of Biology

Diabetes and septic shock related cardiomyopathy research.

Isolated neonatal and adult ventricular cardiomyocytes

Designed experiments to determine signal transduction pathways from LPS-induced

cardiomyopathy. Looked into the LPSD/Ran mutation could diminish LPS effect on heart by

shuttling NFB from the nucleus

Lead for creating SOPs and training for new students in the lab.

Graduate Teaching Associate (19992000)

Taught varied sections of lab class for non-major students.

Created, administered and graded exams, assignments and quizzes.

Explained and aided students with experiments.

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