Assistant Medical

Demirkan B. G rsel Ph.D.

Research Assistant Professor

Department of Neurological

Surgery

Laboratory for Translational Brain Tumor and Stem Cell

Research

Weill Medical

College

*** * **** ******, Whitney

601

New York, NY 10021

Cell Ph# 240-***-****

Email: abooib@r.postjobfree.com

abooib@r.postjobfree.com

EDUCATION

B.Sc. Hacettepe University Ankara, TURKEY (1981-

1987)

M.Sc. University of Maryland at Baltimore, MD, USA

(1989-1992)

Ph.D. University of Bordeaux I, FRANCE (1997-2002)

Postdoctoral Fellow National Cancer Institute, Frederick, MD,

USA (2003-2007)

Postdoctoral Associate Medical College of Cornell University, NY, USA

(2007-2011)

Research Assistant Prof. Medical College of Cornell University, NY, USA

(2011-2012)

PROFESSIONAL POSITIONS

Research Assistant Professor 2011-Present

Weill Cornell Medical College of Cornell University

Neurological Surgery Department

Brain Tumor and Stem Cell Research Laboratory

New York, NY USA

Postdoctoral Associate 2007-2011

Weill Cornell Medical College of Cornell University

Neurological Surgery Department

Brain Tumor and Stem Cell Research Laboratory

New York, NY USA

Postdoctoral Fellow 2003-

2007

National Cancer Institute

Mouse Cancer Genetics Program

Frederick MD, USA

Research Assistant 1995-

1997

The Wistar Institute

Molecular Genetics Program

Philadelphia PA, USA

Research Associate 1992-

1995

Georgetown University

Department of Pediatrics

Genetic Division, Medical School

Washington DC, USA

Research Assistant 1991-

1992

University of Maryland at Baltimore

Department of Biochemistry

Baltimore MD, USA

Research Assistant 1990-

1991

University of Maryland at Baltimore

Department of Microbiology

Baltimore MD, USA

Research Assistant 1989-

1991

University of Maryland at Baltimore

Department of Microbiology

Baltimore MD, USA

PROFESSIONAL MEMBERSHIPS

American Society for Microbiology 1989-

1994

(National and Baltimore Chapters)

Graduate Student Association 1991-1992

Department of Biochemistry (Department representative)

HONORS AND AWARDS

NCI Spring Research Festival 2006

Cancer Research Training Award NCI 2003

CURRENT AND PAST INSTITUTIONAL RESPONSIBILITIES AND PERCENT EFFORT

Current (Cornell)

Teaching: 20%: involving training, research curriculum activities and

assist 3rd year medical students, neurosurgery

residents, summer students, technicians and

research fellows

Translational/Clinical Research: 10%: provide and report preclinical data

to physicians (neurosurgeons and radio

oncologists) for clinical trials

Administrative duties: 10%: lab management and administrative duties

Research: 70%: (basic science and preclinical research

included)

Total: 100%

Past (NCI)

Teaching: 10%: involves training, research curriculum activities and

assist undergraduate and summer students

Translational/Clinical Research: 0%

Administrative duties: 0%

Research: 90%: basic science

Total: 100%

RESEARCH SUPPORT

Current (Cornell): Grants (NIH RO1), PI: John A. Boockvar M.D.

Past (NCI): Cancer Research Training Award and Intramural Research Program

of NIH, NCI; project # ZIA BC 010540. PI: Karlyne M. Reilly Ph.D.

PARTICIPATED KEY DISCIPLINES OVER THE LAST 15 YEARS

Cell Biology, Molecular Biology (Cloning), Microbiology, Genetics,

Biochemistry, Cancer Research (Oncology, Neuro-Oncology), Translational

Science (Drug discovery, Pre-clinical), Cancer Cell Signal Transduction,

Brain Tumor Stem Cell Research, Animal Models and Fluorescence Microscopy

EXPERIMENTAL AND TECHNICAL COMPETENCY

Cellular and Molecular Biology

*Generation of patient-derived Glioblastoma Multiforme (GBM) stem-like

cells

*Generation of primary mouse astrocytes from double knock-out (NF1:p53)

mouse

*MACS sorting

*FACS (limited experience)

*Transfections, transductions and infections using plasmids, Adeno-

associated virus type1/2 and lentivirus on GBM patient stem

cells, mouse primary astrocytes, Hek293, U87, LN229, U373, NIH3T3, C6,

HUVEC to establish transient and stable cell clonal lines

*Cell differentiation, proliferation, migration (2D, 3D), invasion, soft

agar, angiogenic assays (tube formation and sprout assay) and cytoskeletal

reorganizational structural studies

*DNA, RNA, PCR (rt-pcr), cloning (DNA ligation, transformation)

*Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Biochemistry, Protein Biology and Signal Transduction

*Protein profiling, isolation, purification, receptor/ligand cell

stimulation, tyrosine/serine/protein kinase activity assays. Pulse-chase

ligand stimulation/inhibition experiments, Western blot analysis,

immunoprecipitation.

Pre-clinical/Drug Studies and Biomarker Targeting

*In vitro: Application of candidate drugs on conventional and patient-

derived brain tumor stem cells. Study on the effect of potential compounds

on cell signaling, phenotypic changes and the modulation on specific

biomarkers.

In vivo: Drug delivery by intra-peritoneal and intra-arterial applications

followed by analysis of tumor growth, survival and examination of tissues

at transcriptional and translational levels.

Animal Models

*Animal surgery (tumor resection and tissue analysis), subcutaneous,

intracranial (limited experience) injections for establish xenograft and

allograft mouse models.

Microscopy

*Bright field (Nikon, Zeiss and Leica), Fluorescence (Leica, Nikon and

Zeiss) and Confocal (limited experience).

BIBLIOGRAPHY

1. Berry N, G rsel DB, Banu M, Santillan A, Foley C, Burkhardt JK,

Boockvar JA. Evaluation of intra-arterial Bevacizumab delivery on

tumor growth, oncogenic signaling and stem cell microenvironment in a

human GBM xenograft. (Manuscript in preparation).

2. G rsel DB, Schlaff CD, Berry N, Burkhardt JK, Boockvar JA.

Bevacizumab induces apoptosis by activation of p53 signaling pathway

in vitro in patient-derived GBM stem-like cells. (Manuscript in

preparation).

3. G rsel DB, Kobylarz K, Berry N, Banu M, Marongiu R, Burkhardt JK,

Kaplitt MG, Rafii S, Boockvar JA. Tight regulation between cell

survival and programmed cell death in GBM stem-like cells by

EGFR/GSK3b/PP2A signaling. (Submitted).

4. G rsel DB, Schlaff CD, Boockvar JA. Trials and tribulations of

cancer immunotherapy: the dendritic cell vaccine shows promise in a

phase I glioblastoma multiforme trial. Neurosurgery. 2012

Dec;71(6):N19-21.

5. Gursel DB, Schlaff CD, Boockvar JA. EGFR Targeted Inhibition

Resistance: Compensatory Activation of ERBB Family Members in

Glioblastoma Cancer Stem-Like Cells Promotes Proliferation.

Neurosurgery. 2012 Oct;71(4):N17-8.

6. Burkhardt JK, Santillan A, Hofstetter CP, Christos P, Berry N, Shin

BJ, Foley CP, G rsel DB, Ballon DJ, Gobin YP and Boockvar JA. Intra-

Arterial Bevacizumab with blood brain barrier disruption in a

glioblastoma xenograft model. J Exp Ther Oncol 2012;10(1):31-37

7. Berry N, Gursel DB, Boockvar JA. Getting a neural stem cell from a

fibroblast. Neurosurgery 2012 Aug;71(2):N26-8.

8. Gursel DB, Berry N, Boockvar JA. Therapeutic stem cells encapsulated

in a synthetic extracellular matrix selectively kill tumor cells,

delay tumor growth, and increase survival in a mouse resection model

of malignant glioma. Neurosurgery 2012 Jun;70(6):N17-9.

9. Stessin AM, Gursel DB, Schwartz A, Parashar B, Kulidzhanov FG, Sabbas

AM, Boockvar J, Nori D, Wernicke AG. FTY720, sphingosine 1-phosphate

receptor modulator, selectively radioprotects hippocampal neural stem

cells. Neurosci Lett. 2012 May;16;516(2):253-8.

10. Berry N, Gursel DB, Boockvar JA. Notch inhibition via micro-RNA

blocks glioma development. Neurosurgery. 2012 Apr;70(4):N20-2.

11. Hofstetter CP, Burkhardt JK, Shin BJ, G rsel DB, Mubita L, Gorrepati

R, Brennan C, Holland EC, Boockvar JA. Protein phosphatase 2A

mediates dormancy of glioblastoma multiforme-derived tumor stem-like

cells during hypoxia. PLoS One. 2012;7(1):e30059. Epub 2012 Jan 11.

12. Gursel DB, Berry N, Boockvar JA. The contribution of Notch signaling

to glioblastoma via activation of cancer stem cell self-renewal: the

role of the endothelial network. Neurosurgery 2012 Feb;70(2):N19-21.

13. Berry N, Gursel DB, Boockvar JA. Direct conversion of human

fibroblasts to functional neurons in one step. Neurosurgery 2011

Dec;69(6):N18.

14. G rsel DB, Shin BJ, Burkhardt JK, Kesavabhotla K, Schlaff CD and

Boockvar JA. Glioblastoma Stem-Like Cells-Biology and Therapeutic

Implications. Cancers 2011; 3:2655-2666.

15. G rsel DB, Connell-Albert YS, Tuskan RG, Anastassiadis T, Walrath

JC, Hawes JJ, Amlin-Van Schaick JC, Reilly KM. Control of proliferation

in astrocytoma cells by the receptor tyrosine kinase/PI3K/AKT

signaling axis and the use of PI-103 and TCN as potential anti-

astrocytoma therapies. Neuro Oncol 2011; June;13(6):610-21.

16. G rsel DB, Beyene RT, Hofstetter C, Greenfield JP, Souweidane MM,

Kaplitt M, Arango-Lievano M, Howard B, Boockvar JA. Optimization of

glioblastoma multiforme stem cell isolation, transfection and

transduction. J Neurooncol 2011; Feb;19.

17. Howard BM, G rsel DB, Bleau AM, Beyene RT, Holland EC, Boockvar JA.

EGFR signaling is differentially activated in patient-derived glioblastoma

stem cells. J Exp Ther Oncol 2010;8(3):247-60.

18. Turbyville TJ, G rsel DB, Tuskan RG, Walrath JC, Lipschultz CA,

Lockett SJ, Wiemer DF, Beutler JA, Reilly KM. Schweinfurthin A

selectively inhibits proliferation and Rho signaling in glioma and

neurofibromatosis type 1 tumor cells in a NF1-GRD-dependent manner.

Mol Cancer Ther 2010;May 9(5):1234-43.

19. Lemiere S, Azar R, Belloc F, G rsel D, Pyronnet S, Bikfalvi A,

Auguste P. Overexpression of high molecular weight FGF-2 forms

inhibits glioma growth by acting on cell-cycle progression and protein

translation. Exp Cell Res 2008;Dec 10;314(20):3701-11.

20. Bleau AM, Howard BM, Taylor LA, G rsel D, Greenfield JP, Lim Tung

HY, Holland EC, Boockvar JA. New strategy for the analysis of phenotypic

marker antigens in brain tumor-derived neurospheres in mice and

humans. Neurosurg Focus 2008;24(3-4):E28.

21. G rsel DB and Reilly KM. Models of Astrocytoma. Drug Discovery

Today: Disease Models 2005; 2:77-83 (Volume 2, Issue 1 -

ScienceDirect).

22. Auguste P, G rsel DB, Lemi re S, Reimers D, Cuevas P, Carceller F, Di

Santo JP, Bikfalvi A. Inhibition of fibroblast growth factor/fibroblast

growth factor receptor activity in glioma cells impedes tumour growth

by both angiogenesis-dependent and - independent mechanisms. Cancer

Research 2001; Feb 15;61(4):1717-26.

23. Lieberman PM, Ozer J, G rsel DB. Requirement for transcription

factor IIA (TFIIA)-TFIID recruitment by an activator depends on promoter

structure and template competition. Mol Cell Biol. 1997;

Nov:17(11):6624-32.

BOOK CHAPTER

1. Auguste P, G rsel DB, Bikfalvi A. The role of FGF-2 in angiogenesis

and neoangiogenesis. "Fibroblast growth factor in the cardiovascular

system" Editor: P. Cuevas, I. Holzapfel Verlag Publishers, Munich

2002, pg:183-205.

ABSTRACTS AND PRESENTATIONS

1. Demirkan B. Gursel, Nicholas Berry, Keith Kobylarz, Jan-Karl

Burkhardt, Cody Schlaff, Matei Banu, Shahin Rafi, John A. Boockvar.

The regulation of proliferation and programmed cell death in patient

gbm stem-like cells by EGFR/GSK3b/PP2A signaling. Congress of

Neurological Surgeons Annual Meeting, Chicago, IL October 2012.

2. Nicholas Berry, Matei A Banu, Demirkan B. Gursel, Alejandro

Santillan, Conor Foley, Jan-karl Burkhardt, John A. Boockvar.

Evaluation of intra-arterial Bevacizumab delivery on tumor growth,

oncogenic signaling, and stem cell

microenvironment in a human GBM Xenograft. Congress of Neurological

Surgeons Annual Meeting, Chicago, IL October 2012.

3. Demirkan B. G rsel, Benjamin Shin, Jan-Karl Burkhardt, John Andrew

Boockvar. Bevacizumab induces apoptosis and p53 overexpression in

Glioblastoma stem cells. Congress of Neurological Surgeons Annual

Meeting, Washington, DC, October 2011.

4. Nima Soltanzad, Demirkan B. Gursel, John A. Boockvar. MGMT Gene

Methylation in Human Glioblastoma Stem Cells. Congress of

Neurological Surgeons Annual Meeting, Washington, DC, October 2010.

5. Christoph Hofstetter, Demirkan G rsel, Lynn Mubita, Eric Holland,

John Boockvar. Hypoxia induces protein phosphatase 2A enzymatic

activity in glioblastoma multiforme. The 15th Annual Scientific

Meeting of the Society for Neuro-oncology, Montreal, Canada November

2010.

6. David W. Infanger, Brina S. Lopez, S. Chris Liu, Demirkan B. G rsel,

John A. Boockvar, Claudia Fischbach-Teschl. Microenvironmental

control of GBM stem cells

in the perivascular niche. Rockefeller Research Day, 2010.

7. Infanger DW, Liu SC, G rsel D, Boockvar JA, and C Fischbach.

Engineered 3D scaffolds model endothelial cell-tumor stem cell

signaling in

glioblastoma multiforme. Biomedical Engineering Society Conference,

Austin, Texas 2010.

8. Beyene R, G rsel DB, Boockvar J. Optimization of Glioblastoma

Multiforme Stem Cell Isolation and Transfection. Congress of Neurological

Surgeons Annual Meeting. New Orleans, LA, October 2009.

9. Jennifer Moliterno, Robel Beyene, Xiaofei Hu, Demirkan B. G rsel,

Brian Howard, John Boockvar. EGFR activation suppresses the

expression of the catalytic subunit of PP2A in human glioblastoma

multiforme. The New England Neurological Society Meeting, June 2009.

10. John A. Beutler, Thomas J.Turbyville, Jeffery D. Neighbors, Nolan

Mente, David F. Wiemer, Demirkan B. G rsel, Karlyne M. Reilly, Thomas

G. McCloud, Johnson Jato, Anne Monks, Erik Harris and James B.

McMahon. Development of the Schweinfurthins for Glioblastoma. AACR-ACS

Meeting. San Diego, CA, 2007.

11. Krishan Kumar, G rsel DB, Kristy Fox and Karlyne M. Reilly.

Astrocytoma initiation and progression in NPCis in vitro mouse model.

National Neurofibramatosis Foundation International Consortium for the

Molecular Biology of NF1, NF2 and Schwannomatosis. Aspen CO, May

2006.

12. Thomas J. Turbyville, G rsel DB, Karlyne M. Reilly, James B. McMahon,

John A. Beutler. Schweinfurthin A: Molecular and morphological

investigations in glioma. Pharmacology Meeting. Baltimore, MD, 2006.

13. John A. Beutler, Thomas J. Turbyville, Jeffrey D. Neighbors, David F.

Wiemer, G rsel DB, Karlyne M. Reilly, Thomas G. McCloud, Johnson Jato,

and James B. McMahon. The Schweinfurthins: Development of a plant-

derived anticancer lead. NCI Staff Scientists Retreat. Gaithersburg,

MD, 2006.

14. Arnold AC, G rsel DB, Cho EH, Lockett SJ, Reilly KM. Measuring

migration potential of astrocytes in three dimensions. 97th Annual

meeting of AACR. Washington DC, 2006.

15. G rsel DB, Robert G.Tuskan, Kristi Fox, Melinda Hollingshead, Alma

Arnold, Edward Cho, Stephen Lockett and Karlyne M. Reilly. Building a

mouse model of glioblastoma for testing experimental therapeutics.

4th Annual Molecular Targets Faculty Retreat. Gaithersburg, MD, 2005.

16. G rsel DB, Reilly KM. The role of Epidermal Growth Factor signaling

in tumors associated with Neurofibromatosis type I. NCI Mouse Genetics

Retreat. Cumberland, MD, 2004.

17. G rsel DB, Redman RC, Tuskan RG, Cho EH, Lockett SJ, Reilly KM.

Epidermal growth factor receptor signaling in mouse model of nervous

system tumors associated with Neurofibromatosis type I. National

Neurofibramatosis Foundation International Consortium for the

Molecular Biology of NF1, NF2 and Schwannomatosis. Aspen CO, May

2004.

18. G rsel DB, Auguste P, Reimers D, Cuevas P, Bikfalvi A. FGF signaling

in tumor cells participates tumour growth by angiogenesis dependent

and independent mechanisms. European School of Hematology (ESH)

Annual meeting "Angiogenesis and tumors"; Paris, FRANCE 1999.

19. G rsel DB, Blomberg LA, Flor A, Garnica A, Chan WY, Rennert OM.

Characterization of a cDNA clone from human kidney sharing high

homology with the cDNA of placental sphingomyelinase. American

Society of Human Genetics, 44th Annual Meeting Abstract; Vancouver,

CANADA 1994.

20. G rsel DB, Delisle AL. Prophage induction effect of Mitomycin C on

temperate phages of oral Veillonella species. University of Maryland

at Baltimore, Graduate Student Association (GSA) Annual meeting;

Baltimore MD, USA 1991.

References

John A. Boockvar, MD

Alvina and Willis Murphy Assistant Professor of Neurological Surgery

Head, Laboratory for Translational Stem Cell Research

Department of Neurological Surgery

Weill Medical College of Cornell University

525 E. 68th Street, New York, NY 10065

Phone: 212-***-****

Fax: 212-***-****

E-Mail: abooib@r.postjobfree.com

Karlyne M. Reilly, Ph.D.

Head, Genetic Modifiers of Tumorigenesis Section

Mouse Cancer Genetics Program

National Cancer Institute

Building 560, Room 32-31B

Frederick National Lab

Frederick, MD 21702-1201

Phone: 301-***-****

Fax: 301-***-****

E-mail: abooib@r.postjobfree.com

Tommy J. Turbyville, Ph.D.

Scientist

Optical Microscopy and Analysis Laboratory

National Cancer Institute at Frederick

Post Office Box B

Frederick, MD 21702

Phone: 301-***-****

E-Mail: abooib@r.postjobfree.com

Armando Flor, M.D., F.A.A.P.

Senior Director, Biopharm Translational Medicine

Biopharm R&D

GlaxoSmithKline

UM 2291

709 Swedeland Road

King of Prussia, PA 19406

Phone (Office): 610-***-****

Phone (Mobil): 215-***-****

E-Mail: abooib@r.postjobfree.com

Citizenship: USA

Languages: English, French and Turkish

Preparation of CV: February 27, 2013

Contact this candidate