Years Experience Construction

Dongsheng Guo, PhD

*** ***** ***, ***#**

Chicago, IL 60130 *********@*****.*** Cell: 708-***-****

QUALIFICATIONS

. PhD in molecular biology and biochemistry with 8 years experience.

. Specialist in viral genomic analysis by making recombinant viruses to

study virus replication, virus-host interaction and vaccine.

. Specialist in gene delivery to the liver using recombinant Adenovirus

by tail vein injection.

. 4 years experience in lab management and undergraduate student and

technician supervising and training.

. Willing to travel and relocate.

. U.S. Permanent Resident.

TECHNICAL SKILLS

. Virology:

Recombinant virus construction, Virus infection, Virus isolation and

culture, Virus purification, Capsid purification, Virus titration by

plaque assays, One-step growth curve.

. Molecular Biology:

Subcloning, PCR, RT-PCR, q-PCR, PCR mutagenesis, Artificial Bacterial

Chromosome (BAC) Construction, genetic mice generation, Northern blot

and Southern blot.

. Biochemistry:

Protein Expression & Purification, Western blot, Co-IP, Cell-based

ELISA, Radio-ligand binding assay, Protein localization. GST pulldown,

Gel Shift, EMSA, ChIP, Immunohistochemistry, H&E staining.

. Cell Biology:

Tissue Culture, Transfection and Infection, Stable Cell Line

Development, Live Cell Image, Cell Proliferation Assay and Flow

Cytometry. Electron & Fluorescence Microscopy & Confocal,

Immunohistochemistry. Immunofluorescence; Immunofluorescence.

. Microscopy:

Electron & Fluorescence Microscopy; Confocal.

. Animal work:

Mouse and rat breeding, drug treatment, Virus injection by tail vein.

. Excellent written and oral communication skills in English and

Mandarin.

. Excellent skills involving data analysis and presentation using

various software, such as Microsoft

Office, Prism, Photoshop, Illustrator. Also knowledgeable in

specialized programs involving protein and DNA analysis software.

PROFESSIONAL EXPERIENCE

03/2010 - Present: Research Assistant Professor in Department of

Microbiology and Immunology, Feinberg School of Medicine, Northwestern

University, USA.

. Working on Herpesvirus genomic analysis by making recombinant viruses

to study virus replication, virus-host interaction and vaccine.

05/2004 - 03/2010: Postdoctoral fellow in Department of Pathology, Feinberg

School of Medicine, Northwestern University, USA.

. Established gene delivery to the mice liver using recombinant

adenovirus by tail vein injection to study gene regulation.

EDUCATION

. 02/2001- 01/2004: Doctorate of Philosophy in Molecular Biology and

Biochemistry, College of Life Science, Zhejiang University. China.

. 09/1998 - 01/2001: Master of Science in Zoology, College of Life Science,

Hebei Normal University, China.

. 09/1993 - 07/1997: Bachelor of Science in Biology, College of Life

Science, Hebei Normal University, China.

PRESENTATIONS (SELECTIVE)

. Dongsheng Guo. Herpesvirus genomic analysis. Virology Group meeting in

the department of microbiology and Immunology. Mar 31, 2011.

. Dongsheng Guo. A Potential protein candidate which is essential for PRV

replication. Virology Group meeting in the department of microbiology and

Immunology. Sep 30, 2010.

. Dongsheng Guo. Peroxisome proliferator-activated receptor (PPAR)-binding

protein (PBP): a crucial coactivator for constitutive androstane receptor

(CAR). Pathology Research Conference. Oct 2, 2006

PEER-REVIEWED PUBLICATIONS (SELECTIVE)

. Mindy Leelawong, Dongsheng Guo, Greg A Smith. A physical link between

the pseudorabies virus capsid and the nuclear egress complex. Journal

of Virology. 2011 August 31 (Online).

. Dongsheng Guo, Joy Sarkar, Kelly Suino-Powell, Yong Xu, Kojiro

Matsumoto, Yuzhi Jia, Songtao Yu, Sonal Khare, Kasturi Haldar, M.

Sambasiva Rao, Jennifer E. Foreman, Satdarshan P.S. Monga, Jeffrey M.

Peters, H. Eric Xu, and Janardan K. Reddy. Induction of nuclear

translocation of CAR by PPARalpha synthetic ligands in mouse liver. J

Biol Chem, 282 (50): 36766-76, 2007.

. Dongsheng Guo, Joy Sarkar, Mohamed R. Ahmed, Navin Viswakarma, Yuzhi

Jia, Songtao Yu, M. Sambasiva Rao and Janardan K. Reddy. Peroxisome

proliferator-activated receptor (PPAR)-binding protein (PBP) but not

PPAR-interacting protein (PRIP) is required for nuclear translocation

of constitutive androstane receptor in mouse liver. Biochem Biophys

Res Commun, 347: 485-495, 2006.

. Joy Sarkar, Chao Qi, Dongsheng Guo, Mohamed R. Ahmed, Yuzhi Jia, N

Usuda, Navin Viswakarma, M. Sambasiva Rao and Janardan K. Reddy.

Transcription coactivator PRIP, the peroxisome proliferator-activated

receptor (PPAR)-interacting protein, is redundant for the function of

nuclear receptors PParalpha and CAR, the constitutive androstane

receptor, in mouse liver. Gene Expression, 13 (4-5): 255-269, 2007.

. Sailesh Surapureddi, Navin Viswakarma, Songtao Yu, Dongsheng Guo, M.

Sambasiva Rao and Janardan K. Reddy. PRIC320, a transcription

coactivator, isolated from peroxisome proliferator-binding protein

complex. Biochem Biophys Res Commun, 343: 535-543, 2006.

. Janardan K. Reddy, Dongsheng Guo, Yuzhi Jia, Songtao Yu and M.

Sambasiva Rao. Nuclear receptor transcriptional coactivators in

development and metabolism. Advances in Developmental Biology, 16: 389-

420, 2006.

. Yuzhi Jia, Grace L. Guo, Sailesh Surapureddi, Joy Sarkar, Chao Qi,

Dongsheng Guo, Jun Xia, Papreddy Kashireddi, Songtao Yu, Young-Wook

Cho, M. Sambasiva Rao, Byron Kemper, Kai Ge, Frank J. Gonzalez, and

Janardan K. Reddy. Transcription coactivator peroxisome proliferator-

activated receptor-binding protein/mediator 1 deficiency abrogates

acetaminophen hepatotoxicity. PNAS 102: 125**-*****, 2005

. Dongsheng Guo, Chenggang Zhu, Yaozhou Zhang and Xiangfu Wu. Expression

of Human IFN?2b-THY?1 Fused Gene in Bombyx mori Cell and Study of Its

Bioactivity. Progress in Biochemistry and Biophysics, 30: 803-807,

2003.

INVITED MANUSCRIPT AND GRANT REVIEWS (SELECTIVE)

. Applied Immunohistochemistry & Molecular Morphology

. Diagnostic Pathology

. Journal of Hematology & Oncology

. The Journal of Experimental & Clinical Cancer Research

PROFESSIONAL AFFILIATIONS

. Sigma Xi (The Scientific Research Society)

. AAAS (The American Association for the Advancement of Science)[pic][pic]

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