Development Process

Fei Wang

**** ****** **** ***, ******, NC***** 919-***-**** **************@*****.***

SUMMARY

Research professional with over 10 years of experience in many aspects of process development, technical

transfer, and scale-up

EDUCATION

MS/Medicinal Chemistry, School of Pharmacy, University of North Carolina at Chapel Hill

BS/Medicinal Chemistry, Shenyang Pharmaceutical University, Shenyang, China

KEY RESEARCH AND MANAGEMENT SKILLS

Protein Expression and Purification

AKTA/UNICORN software

UF/DF( ultrafiltration/diafiltration)

Ion-Exchange, Affinity, and Gel filtration chromatography

Column Packing

Precipitation and ultra-centrifugation

SDS-PAGE

Western Blot Analysis

ELISA

DOE (with JMP software)

Lab Management

PROFESSIONAL EXPERIENCE

Research Associate Contractor (November 2011 – September 2012)

BD Diagnostics, RTP, NC

Facilitated the development of diagnostic kit for diagnosis of Sepsis in whole blood samples

Identification and evaluation of Sample Prep Technologies to enable earlier detection, identification and

characterization of pathogenic microorganisms under BSL-2 working environment

Personally drove the evaluation and characterization of a novel technology that proved challenging technical

milestones could be met. Goals were met on-time. Worked productively with a cross-function team to integrate

the technology into a workflow that demonstrated a complete sample to result solution was feasible.

Present data/project update to company management and inter-departmental teams

Senior Associate Process Development Scientist II

Grifols/Talecris Biotherapeutics Inc, Raleigh, NC (March 2009 – September 2011)

Contributed to the hemostasis product research for plasma derived Factor III (koate) and recombinant Factor VIII to

provide treatments for hemophilia A

Served as the only resource for developing and optimizing purification process for recombinant Factor

VIII protein using precipitation, affinity, ion-exchange, and gel filtration chromatography, delivered

highly purified recombinant FVIII protein for pre-clinical study and toxicity study

Tested different virus clearance reagents (PS80 and TNBP) using a gel filtration chromatography method to

support Koate manufacturing process

Facilitated the development of scaled-down model of commercial manufacturing process for plasma-derived

Factor VIII from cyro precipitate. Developed filtration and chromatography unit operations to improve

robustness, throughput, and product recovery

Executed experiments in close collaboration with other team member to establish purification process for

recombinant Plasmin using UF/DF(ultrafiltration/diafiltration) and ion-exchange chromatography

Developed analytical methods like SDS-PAGE, protein quantification (BCA), Western Blots, ELISA, that

supported protein purification process

Prepared batch records and reports that met cGMP requirement

Epizyme Inc, Chapel Hill, NC (March 2008 – March 2009)

Senior Research Associate

Contributed to the discovery and development of drugs for new classes of epigenetic targets to provide novel

treatments for cancer

Facility set up and maintenance

Served as the primary resource for expression and purification of recombinant protein from Ecoli and insect

cells using affinity and gel filtration chromatography

Isolated enzyme substrate from chicken blood using sucrose gradient ultra centrifugation method

Developed bioassays to explore epigenetic enzymes and proteins such as histone methyltransferases

Research Technician III/Research Specialist (November 1998 – August 2007)

University of North Carolina at Chapel Hill

Performed DNA cloning and expression and purification of recombinant protein from Ecoli f to support antibody

production. The purified antibody was used for western blot, ELISA, and immunostaining

Studied genetic analysis of 41c and the 2R heterochromatin of Drosophila melanogaster, correlated the genetic

and physical map using PCR technique and DNA sequence analysis. The study resulted in a peer reviewed

publication

Training

Biopharmaceutical Process Development (NCSU-BTEC Summer 2010 )

Fundamentals of Cell Culture (NCSU-BTEC 2011 Customized Course for Industry groups)

PEER-REVIEWED PUBLICATIONS

Fox DT, Homem CC, Myster SH, Wang F, Bain EE, Peifer M. (2005) Rho1 regulates Drosophila adherens junctions

independently of p120. Development, 132(21), 4819-31

Myster SH, Wang F, Cavallo R, Christian W, Bhotika S, Anderson CT, and Peifer M.(2004) genetics and

Bioinformatic Analysis of 41C and the 2R Heterochromatin of Drosophila melanogaster: A Window on the

Heterochromatin –Euchromatin Junction. Genetics 166, 807-822

Wang F, Warren AE, Barnes CR and Hall IH. (1998) Cytotoxicity of poly(phenolic) sulfonates and their sodium salts

in murine and human tumor cell lines, Metal-Based Drugs 5, 147-161

Hall IH, Izydore RA, Barnes BJ, Wang F, and Warren AE. (1998) Hypolipidemic triazolidine- 3,5-diones, 3,5-

pyrazolinediones, 3, 5-isoxazolinediones, 1,3,5,-triazabicyclo[3.1.0.]hexane-2,4- diones as HMG-CoA Reductase,

ACAT, GPAT, and PP inhibitors and NCEH activators. Recent Research Development in lipid 1, 297-308

Burnham BS, Gupton JT, Wang F and Hall IH. (1998) Cytotoxicity of Substituted Alkyl-3,4- bis(4-

methoxyphenyl)pyrrole-2-carboxylates in L1210 Lymphoid Leukemia Cells.” Arch der Pharm Med. Chem. 337-341,

ABSTRACTS:

Tony J.C. Harris, Fei Wang and Mark Peifer. Adherens junction-dependent and independent processes regulate

epithelial polarity and structure during early Drosophila development, 45th Annual Drosophila Research Conference,

2004, Washington, DC

Donald T.Fox, Steve H Myster, Fei Wang, and Mark Peifer. Drosophila p120 catenin plays a supporting role in cell

adhesion, but is not an essential adherens junction component, 45th Annual Drosophila Research Conference,

2004, Washington, DC

Wang F and Thomas MJ. Estrogen Inhibits CAMP-Mediated Induction of Insulin-Like Growth Factor (IGF-1) Gene

Expression in Mouse Osteoblast MC 3T3-E1 Cells. Abstract of The Endocrine Society’s 83nd Annual Meeting, 2001

Wang F and Thomas MJ. Evaluation of Growth Hormone(GH) Induced DNase1Hypersensitive Sites in The Rat and

Human Insulin-Like Growth Factor (IGF)-1 Genes In Vitro. Abstract of The Endocrine Society’s 83nd Annual

Meeting, 2001

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