Assistant Human

Dipti (Gupta) Mangal, Ph. D.

***, **** ****** ****, *** H13

Media, PA, 19063

Cell No. 860-***-****

***********@*****.***

Objectives: To obtain a challenging and responsible position in the area of

bioanalysis/biotransformation within the department of drug

metabolism and pharmacokinetics

Qualification Highlights

. HPLC and LC-MS\MS method development and validation.

. HPLC, and MS instruments maintenance and troubleshooting.

. Small molecule structure elucidation and identification

using LC/MS/MS.

. Good understanding of P450 and non-P450 mediated reaction

mechanisms.

. Knowledge of in vitro and in vivo biotransformation studies.

. Immunodepletion, to remove the high abundance protein.

. Strong cation exchange chromatography to separate the

peptides..

. Cell culture.

. Organic synthesis (including multistage reactions) of

heterocyclic compounds.

. Rapid extraction, separation, purification and

quantification of a wide variety of complex organic

molecules of biological importance from biofluids.

. Knowledge of pharmacokinetics.

. Spectroscopic methods 1H-NMR, UV-VIS, IR

Professional Experience

2009 to present Postdoctoral fellow, School of Veterinary Medicine, New

Bolton Center, University of Pennsylvania

. Developed and validated LC-MS/MS methods to analyze the

prostaglandins metabolites from equine plasma samples as

biomarkers of illegal use of glucocorticoids.

. Identification of testosterone and methyl prednisolone

metabolites in equine S-9 cellular fraction, Liver

microsomes and urine with LC-MS/MS. (Ion trap and Thermo

Exactive).

. Develop a LC-MS/MS method to identification and

quantification of proteins from equine plasma by using

proteomic approach.

2005 to 2009 Postdoctoral fellow, University of Pennsylvania,

Department of Pharmacology

. Developed LC-MS/MS methods to identify and to detect novel

8-oxo deoxyguanosine and heptenone etheno deoxyguanosine

adducts from biological samples (DNA and Urine) as

biomarkers for oxidative stress.

. Developed liquid-liquid extraction, SPE, and sample

preparation methods for 8-oxo-deoxyguanosine in cells and

urine.

. Identified and structure elucidation of benzopyrene

metabolites in cells with LC/MS/MS (ion trap and triple

quadrapole).

. Synthesized antigen for preparation of 8-oxo-

deoxyguanosine antibodies.

. Communicated and presented data and experimental

results in the

. scientific conferences.

1999-2005 Graduate student and research assistant, Dr. B. R. Ambedkar

Centre for Biomedical Research, University of Delhi.

. Synthesized potent euglycemic and hypolipidemic agents.

. Developed a method for the synthesis of Substituted 1,2,3,4-

Tetrahydroquinoxalin-2-one Analogues of Thiazolidinedione

. Purified organic molecules by chromatographic techniques and

analyzed via modern spectroscopic methods.

. Supervised junior Ph.D. graduate students

1995-1999 Teaching of the chemistry courses to the undergraduate

students.

Supervision experiences

2002-2005 Over sighted responsibilities of 3 junior lab members,

delegated tasks out to 2 junior graduate students, followed up

on their work in progress.

2001-2005 Assisted teaching of the chemistry courses and helped in

designing experiments of organic synthesis for M.S students.

Education

Ph.D. Delhi University, India, Synthetic Organic Chemistry, 2005

Thesis: Synthesis of substituted 1,2,3,4-tetrahydroquinoxaline

analogues of thiazolidinedione: Potent euglycemic and

hypolipidemic activity

M.S. Meerut University, India, Organic Chemistry, 1995

B.S. Meerut University, India, Chemistry, 1993

Publications

DeNicola GM, Karreth FA, Humpton TJ, Gopinathan A, Wei C, Frese K, Mangal

Dipti, Yu KH, Yeo CJ, Calhoun ES, Scrimieri F, Winter JM, Hruban RH,

Iacobuzio-Donahue C, Kern SE, Blair IA, Tuveson DA. Oncogene-induced Nrf2

transcription promotes ROS detoxification and tumorigenesis. Nature, 2011;

475(7354):106-9.

Dipti Mangal, Cornelius E. Uboh, Lawrence R. Soma. Analysis of bioactive

eicosanoids in equine plasma by stable isotope dilution reversed-phase

liquid chromatography/multiple reaction monitoring mass spectrometry, Rapid

Comm. Mass Spectrom. 2011; 25(5):585-98.

Jong-Heum Park, Dipti Mangal, Alexander J Frey, Ronald Harvey, Ian Blair

and Trevor Penning. Aryl Hydrocarbon Receptor Facilitates DNA Strand Breaks

and 8-Oxo-2 '-deoxyguanosine Formation by the aldo-keto reductase product

benzo[a]pyrene-7,8-dione. J. Biol. Chem. 2009 284 (43): 297**-*****.

Dipti Mangal, Jong-Heum Park, Daljit Vudathala Seon Hwa Lee, Trevor Penning

and Ian Blair. Analysis of 7,8-Dihydro-8-oxo-2'-deoxyguanosine in Cellular

DNA during Oxidative Stress. Chem. Res. Toxicol. 2009, 22(5): 788-797

Jong-Heum Park, Dipti Mangal, Kirk Tacka, Amy Quinn, Ronald Harvey, Ian

Blair and Trevor Penning. Evidence for the aldo-keto reductase pathway of

polycyclic aromatic trans-dihydrodiol activation in human lung A549 cells.

Proc Nat. Acad Sci U S A. 2008, 15(19) : 6846-6851.

Carol Shultz, Nisha Palackal, Dipti Mangal, Ronald Harvey, Ian Blair,

Trevor Penning. Fjord-region benzo[g]chrysene-11,12-dihydrodiol and

benzo[c]phenanthrene-3,4-dihydrodiol as substrates for rat liver

dihydrodiol dehydrogenase (AKR1C9): structural basis for stereochemical

preference. Chem. Res Toxicol. 2008, 21(3):668-677.

Hao Jiang; Stacy Gelhaus; Dipti Mangal; Ronald Harvey; Ian Blair; Trevor

Penning. Metabolism of benzo[a]pyrene in human bronchoalveolar H358

cells using liquid chromatography-mass spectrometry. Chem Res Toxicol.

2007 20(9):1331-41.

Dipti Gupta; Narendra Ghosh and Ramesh Chandra. Synthesis of 5-[4-[2-

(6,7-dimethyl-1,2,3,4-tetrahydro-3-oxo-1-

quinoxalinyl)ethoxy]phenyl]methylene]-thiazolidine-2,4-dione and related

compounds: potent euglycemic and hypolipidemic Agents. Bioorg. Med.

Chem. Lett, 2005, 15, 1019-1022.

Narendra Ghosh; Dipti Gupta;Jyoti Chaudhary; Sulekh Chand; Sujata Dass

and Ramesh Chandra. Advances in the chemistry of thiazolidinedione as

antidiabetic agents. J. Ind. Chem. Soc. 2003, 80, 1045-1055.

Professional Conference Presentations

Dipti Gupta; Narendra Ghosh and Ramesh Chandra. Synthesis of 5-[4-[2-(6,7-

Dimethyl-1,2,3,4-tetrahydro-3-oxo-1-quinoxalinyl)ethoxy]phenyl]methylene]-

thiazolidine-2,4-dione and related compounds: potent euglycemic and

hypolipidemic agents.."Gordon Research Conference at Holderness School

Plymouth, New Hamshire USA (11-16th July 2004).

Dipti Mangal; SeoHwa Lee; Daljit Vudathala,; Trevor Penning Ian Blair;

The effect of COMT inhibition on 8-hydroxy-2'-deoxyguanosine levels in

benzo[a]pyrene-7,8-diol-treated human bronchoalveolar cells.Annual

Pharmacology student symposium, University of Pennsylvania, Philadelphia

2005.

Dipti Mangal; SeonHwa Lee; Daljit Vudathala; Trevor Penning, Ian Blair;

The effect of COMT inhibition on 8-hydroxy-2'-deoxyguanosine levels in

benzo[a]pyrene-7,8-dione-treated human bronchoalveolar cells Annual

Conference on Mass Spectrometry and Allied Topics, Seattle WA, 2006.

Dipti Mangal, Jong-Heum Park, Seon Hwa Lee, Trevor M. Penning and Ian

A. Blair; Analysis of 8-Oxo-2`-Deoxyguanosine in Human Bronchoalveolar

Cells by Immunoaffinity Liquid Chromatography/Mass Spectrometry. American

chemical society, Boston MA, 2007

Dipti Mangal,, Seon Hwa Lee, Jong-Heum Park, Clementina Mesaros Trevor

M. Penning and Ian A. Blair; Analysis of 8-oxo-2`-deoxyguanosine on

exposure of Human Bronchoalveolar Cells to Benzo[a]pyrene-7,8-dihydrodiol.

Sixth Annual Frontiers in Cancer Prevention Research Conference,

Philaddelphia PA, 2007

Dipti Mangal,, Seon Hwa Lee, Ian Blair. Lipid mediated DNA damage. Oral

Presentation, Lipidomics workshop Boston, MA, 2007

Dipti Mangal, Angela Y.Wehr, SeonHwa Lee, Clementina Mesaros, Tilo Grosser,

Susanne Fries, Trevor Penning and Ian Blair. Oxidative Stress-Mediated DNA

Damage on Exposure of Cigarette Smoke. American chemical society,

Philadelphia, PA, 2008

Dipti Mangal, Jong-Heum Park, Seon Hwa Lee, Clementina Mesaros, Trevor M.

Penning, Ian A.Blair. Induction of oxidative stress in human lung cells

after exposure to a benzo[a]pyrene metabolite. Annual Conference on Mass

Spectrometry and Allied Topics, Denver, CO, 2008.

Dipti Mangal, Clementina Mesaros, Trevor M. Penning, Ian A.Blair. Analysis

of glutathione and cysteinylglycine conjugates of -anti-7,8-dihydroxy-

9,10-epoxy-7,8,9,10-tetrahydro-B[a]P by Liquid Chromatography/ Tandem Mass

Spectrometry. Annual Conference on Mass Spectrometry and Allied Topics,

Philadelphia PA, 2009.

Ashley Wohler; Dipti Mangal; Clementina Mesaros; Laura Phillips; Ian A.

Blair; Analysis of Urinary 8-Oxo-7,8-dihydro-2`-deoxyguanosine by Liquid

Chromatography/Mass Spectrometry. Annual Conference on Mass Spectrometry

and Allied Topics, Philadelphia PA, 2009.

Dipti Mangal; Cornelius Uboh; Lawrence R Soma; Inhibition of arachidonic

acid (AA) metabolism by glucocorticoids in equine plasma. Annual Conference

on Mass Spectrometry and Allied Topics, Philadelphia PA, 2009.

Membership/Societies

The American Society for Mass Spectrometry (ASMS)

American Chemical Society

References Available upon request

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