JIANG SHUJIA
**** *** *****, **********, ****, U.S.A. 45242
Mobile: 513-***-**** Email: abogg8@r.postjobfree.com
EDUCATION:
Master of Science (MSc).
Biomedical Engineering, Nanyang Technological University, Singapore
Bachelor of Medicine (MD).
Clinical Medicine, China Medical University, P R China
PROFESSIONAL EXPERIENCES:
University of Cincinnati, United States of America Aug.
2004- Present
Postdoctoral fellow /Research Scientist
Under the supervision of professor and scientist, conduct research project for aging heart treatment,
mainly involve in the following areas:
- Isolating and culturing aged and young rat bone marrow mesenchymal stem cells and characterize
them with various methods such as flowcytometry and immunostaining for vitro study.
- Transduce the bone marrow mesenchymal stem cells with adenovirus encoding various genes such
as Akt, Ang-1, and VEGF and study their expression by means of electrophorosis, immunoblotting,
PCR and immunostaining methods.
- labeled the bone marrow mesenchymal stem cells with various labeling method such as retovirus,
adenovirus or fluorescent dye for post-transplantation identification
- Designing and creating aged rat heart infarction models for my research work and conduct vivo
study with treated or non- treated bone marrow mesenchymal stem cells transplantation.
- Processing tissue samples such as cryosectioning, paraffin sectioning, and various staining to
identify the donor cells
- Analyze the research outcomes; write reports and make presentation.
National Heart Centre, Singapore (Attached at Department of Cardiac Thoracic Vascular
Surgery, National University of Singapore)
2001-2004
Research fellow
Under the supervision of professor and scientist, conduct research projects. My main work involved
in the following areas:
- Designing and creating various diseased animal models such as porcine heart ischemic &
infarction model, rodent and rabbit heart infarction model.
- Isolating and culturing various cells such as skeletal myoblasts, bone marrow stem cells, and
cardiomyocytes and maintaining cell lines.
- Carrying out vitro study using molecular methods such as ELISA and electrophoresis, extract
RNA, cDNA synthesis, PCR etc. to analyze the characteristics of cells or tissues.
- Carrying out vivo study for cell transplantation via surgical operation
- Processing tissue samples such as cryosectioning, paraffin sectioning, and various staining to
identify the donor cells
- Analyze the research outcomes; write reports and make presentation.
- To be responsible for the administrative function and maintenance of laboratory which including:
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- Handling of purchase order of equipments and chemicals for research projects.
Maintaining equipments of the laboratory
Clinical Research Institute, National University of Singapore, Singapore
2000-2001
Research Officer
My major duty was to provide core service for researchers of university:
- Process PCR reaction for sequencing analysis
- Cast Long Ranger/Polyacrylamide gel for electrophoresis
- Process various samples on ABI-PRISM377 sequencer to acquire the DNA sequence.
- Analysis of sequencing results
- Report with sequencing results to researchers
- Maintenance of sequencer, laboratory; prepare chemical reagents and buffers
- Provide assistance for immunohistological work such as tissue sectioning and staining
and assist on equipments operation
Pediatric Diagnostic Center, National University Hospital, Singapore
1997-2000
Laboratory Technologist
I mainly worked in laboratory to process patient’s samples for clinical diagnosis, partially worked for
clinical research
- Pulmonary function study for clinical diagnosis
- Running clinical trials for pharmaceutical company
- Process umbilical cord blood sample for screening of G6PD deficiency.
- Using ELISA to process patient sample for allergy test in clinical diagnosis; provide data for
clinical research project.
- Extract DNA from patient’s sample for PCR reaction to determine the presence of EBV virus for
both clinical diagnosis and research.
- Extract protein from various sources such as food, spores and ferns and process them for clinical
allergy test and research project.
- Analysis the results and report to clinical doctors
Anshan General Hospital, P R China
1987-1996
Otolaryngology Specialist Doctor
During this period, I was very well trained in surgical techniques. I mainly worked for diagnosis and
treatment of ENT disease. The major area I involved was:
- First aid on ENT emergency cases such as epistaxis, acute laryngeal obstruction, foreign body of
respiratory tract, pharynx, and esophagus.
- Diagnosis and treatment on common ENT disease like inflammation of ENT, Tumors of ear,
nose, pharyngeal and throat, various kinds deafness, otogenic intracranial Complication, etc.
- Experienced in various complicated surgical operation such as deviation of nasal septum, radical
maxillary sinusotomy, laryngofissure, part or total laryngectomy, neck dissection, plastic
operation of external or middle ear and nose, maxillectomy, etc.
- Actively participated in research focusing on the improvement of operational process and earlier
diagnosis of various malignant tumors.
- As a doctor-in-charge, also included supervision of interns and externs.
DISTICTION
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1. Won the first prize of The Young Investigator Award in the 14 th Annual Scientific Meeting of
Singapore Cardiac Society, March 2-3, 2002.
2. Won the second prize of The Young Investigator Award in the 14 th ASEAN Congress of
Cardiology. Kuala Lumpur, July 17-21, 2002. Malaysia.
OTHERS:
- Since 1998 till 2004, working as a part-time translator of Merck Sharp & Dohme (I.A.) Corp.,
Singapore; I am very familiar with all the formats of clinical research protocols.
- Experienced in setting up new research laboratory.
PUBLICATIONS
Selected articles recently published:
1. Activation of diverse signaling pathways by ex-vivo delivery of multiple cytokines for
myocardial repair. Stem Cells Dev. 2013 Jan 15;22(2):204-15
2. Cardiac progenitors derived from reprogrammed mesenchymal stem cells contribute to
angiomyogenic repair of the infarcted heart. Basic Res Cardiol. 2012;107(6):301
3. Subcellular preconditioning of stem cells: mito-Cx43 gene targeting is cytoprotective via
shift of mitochondrial Bak and Bcl-xL balance. Regen Med. 2012 May;7(3):323-34.
4. Concomitant activation of miR-107/PDCD10 and Hypoxamir-210/Casp8ap2 and their
role in cytoprotection during ischemic preconditioning of stem cells. Antioxid Redox Signal.
2012 Oct 15;17(8):1053-65.
5. MicroRNA-143 is critical regulator of cell cycle activity in stem cells with co-overexpression of
Akt and angiopoietin-1 via transcriptional regulation of Erk5/cyclin D1 signaling. Cell Cycle.
2012 Feb 15;11(4):767-77
6. Non-hypoxic stabilization of HIF-Iα during coordinated interaction between Akt and
angiopoietin-1 enhances endothelial commitment of bone marrow stem cells. J Mol Med (Berl).
2012 Jun;90(6):719-30
7. Activation of IL-11/STAT3 pathway in preconditioned human skeletal myoblasts blocks
apoptotic cascade under oxidant stress. Regen Med. 2012 Jan;7(1):47-57.
8. Cytoprotective and Proangiogenic Activity of Ex-Vivo Netrin-1 Transgene Overexpression
Protects the Heart Against Ischemia/Reperfusion Injury. Stem Cells Dev. 2011 Nov 11. Stem Cells
Dev. 2012 Jul 1;21(10):1769-78
9. Cardiac tumorigenic potential of induced pluripotent stem cells in an immunocompetent host
with myocardial infarction Regen Med. 2011 Mar;6(2):171-8
10. Reprogramming of skeletal myoblasts for induction of pluripotency for tumor-free.Circ Res.
2011 Jun 24;109(1):60-70
11. Sonic hedgehog gene delivery to the rodent heart promotes angiogenesis via iNOS/Netrin-1/PKC
pathway. PLoS ONE 2010; 5(1):e8576.
12. Sca-1+ Stem Cell Survival and Engraftment in the Infarcted Heart Dual Role for Preconditioning-
Induced Connexin-43. Circulation. 2009; 119(19): 2587-96.
13. Ischemic preconditioning augments survival of stem cells via miR-210 expression by targeting
caspase-8-associated protein 2. J Biol Chem. 2009; 284(48):33161-8.
14. Preconditioning promotes survival and angiomyogenic potential of mesenchymal stem cells in
the infarcted heart via NF-kappaB signaling. Antioxid Redox Signal. 2010 Mar 15; 12(6):693-
702
15. IGF-1–overexpressing mesenchymal stem cells accelerate bone marrow stem cell mobilization
via paracrine Activation of SDF-1_/CXCR4 Signaling to Promote Myocardial Repair. Circ Res
2008; 103; 1300-1308
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16. Stable therapeutic effects of mesenchymal stem cell-based multiple gene delivery for cardiac
repair. Cardiovasc Res. 2008 Feb 15; 77(3):525-33
17. Transcriptional profiling of young and old mesenchymal stem cells in response to oxygen
deprivation and reparability of the infarcted myocardium. J Mol Cell Cardiol. 2008 Mar;
44(3):582-96
18. Pharmacologically preconditioned skeletal myoblasts are resistant to oxidative stress and
promote angiomyogenesis via release of paracrine factors in the infarcted heart. Circ Res 2007;
100(4); 545-55
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