RYAN F.OVERCASH

aboffo@r.postjobfree.com

**** **** ***** ** . *******, NC 27613 . 919-***-****

OBJECTIVE AND QUALIFICATIONS:

< To obtain an entry-level scientist position in the

biotechnology/pharmaceutical industry to assist in the development

and/or validation of novel drug therapies.

< Trained in a broad range of molecular biology techniques and

biochemical assays with an emphasis on cell-based functional assays,

immunoassays, and nucleic acid detection or manipulation through PCR.

< Over six years of experience in cell culture as well as animal

models.

< Proven record of proficiency in written and oral communication of

research goals and results.

< Advised and trained several research technicians and graduate

students in laboratory techniques and in the principals of molecular

biology.

EDUCATION:

East Carolina University, Greenville, NC

. PhD, Biochemistry and Molecular Biology

Awarded in October of 2012

. BS, Biology, cum laude

Awarded in June of 2006

RESEARCH EXPERIENCE:

Laboratory of Dr. Maria Ruiz-Echevarria (Advisor)

East Carolina University - Brody School of Medicine

September 2007-October 2012

Graduate student/Researcher

Dissertation: An examination of the functional role of TMEFF2 in

prostate cancer and the translational regulatory mechanisms controlling

its expression

. Investigated the functional role of the TMEFF2 gene in prostate

tumorigenesis using human and mouse immortalized cell lines and

a series of cell-based phenotypic assays.

. Generated and characterized a novel transgenic mouse model with

prostate-specific TMEFF2 over-expression.

. Elucidated a regulatory pathway connecting androgen signaling

with the translation of mRNA transcripts containing upstream

open reading frames.

. Trained research technicians/graduate students in laboratory

techniques and provided guidance for multiple ongoing research

projects.

. Collaborated with other laboratories and regularly presented

data at group meetings to optimize research efficiency.

Graduate laboratory rotations

East Carolina University - Brody School of Medicine

Department of Biochemistry and Molecular Biology: August 2006-

September 2007

Graduate student/Researcher

. Rotated to various laboratories within the department to gain

experience in several types of research as well as exposure to a

variety of laboratory techniques.

. Extensive work was performed analyzing 1) carbohydrate

attachments to KV3 potassium channel proteins through

glycosidase digestion followed by western blot analysis and 2)

protein-DNA binding interactions in the growth hormone (hGH-N)

gene locus through electrophoretic mobility shift assays (EMSA).

Laboratory of Dr. Sam Pennington

East Carolina University - Brody School of Medicine July

2005-August 2006

Laboratory technician (part-time)

. Investigated the effects of maternal dietary fat intake during

gestation on the glucose sensitivity of offspring using a rat

model.

. Measured rat glucose responsiveness through intravenous glucose

tolerance tests.

. Extracted fatty acids from rat tissues and analyzed samples by

gas chromatography.

PUBLICATIONS:

Overcash, R., Chappell, V., Green, T., Geyer C., Asch, A., MJ Ruiz-

Echevarria, (2013) Androgen signaling promotes translation of TMEFF2

in prostate cancer cells via phosphorylation of the ? subunit of the

translation initiation factor 2 (eIF2?). In Press: PLOS One

Ruiz-Echevarria, M., Chen, X., Overcash, R. Ectodomain shedding as a

mechanism to generate cancer biomarkers (Ectomarkers). In: Cancer

Biomarkers. Georgakilas, AG, ed. Boca Raton: CRC Press, 2012: 101-

172.

Chen, X*., Overcash, R*., Green, T., Hoffman, D., Asch, A., MJ Ruiz-

Echevarria, (2011). The tumor suppressor activity of the

transmembrane protein with epidermal growth factor and two follistatin

motifs 2 (TMEFF2) correlates with its ability to modulate sarcosine

levels. J. Biol. Chem. *authors contributed equally to this work

TECHNICAL SKILLS:

. cell culture

. stable protein expression or knockdown in mammalian cells

. cell-based phenotypic assays

. PCR (real-time and endpoint)

. DNA mutagenesis

. cloning

. reporter assays

. DNA and RNA extraction/purification

. Southern blotting

. western blotting

. immunofluorescence

. radioactive labeling

. gas chromatography

. flow cytometry

. transgenic animal construction and genotyping

Honors/ Awards:

Trainee Research Poster Award (2012) -26th Annual Yash P. Kataria

Internal Medicine Research Day

Trainee Research Poster Award (2010) -24th Annual Yash P. Kataria

Internal Medicine Research Day

Doctoral Student Association Travel Award (2010) - 101st annual

American Association for Cancer Research conference in Washington, DC

Inducted into Phi Kappa Phi Honor Society (2006)

REFERENCES:

Maria J. Ruiz-Echevarria, PhD

Assistant Professor

Department of Internal Medicine, Hematology/Oncology

Brody School of Medicine 3E-110

East Carolina University

Tel: 252-***-****

aboffo@r.postjobfree.com

Phillip H. Pekala, PhD

Professor & Chair

Department of Biochemistry & Molecular Biology

Brody School of Medicine 5S-19

East Carolina University

Tel: 252-***-****

aboffo@r.postjobfree.com

Li Yang, PhD

Assistant Professor

Department of Internal Medicine, Hematology/Oncology

Brody School of Medicine 3E-127

East Carolina University

Tel: 252-***-****

aboffo@r.postjobfree.com

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