Igor Ernesto Bruzual Acevedo, PhD
**** ** ******* ** #** Portland Oregon 97219
Email: aboewc@r.postjobfree.com
Phone: 617-***-****
Academic Background
**** *** ********* ******* (2002)
Central University of Venezuela (Universidad Central de Venezuela).
Thesis: “Analysis of Heavy Metals Determinants
on plasmids of Inc HI3and HII”
Master in Cellular Biology (1996), Central University of Venezuela
1996
Research Experience:
Research Associate, Wong Laboratory, Infectious Disease Division,
2010 – present Oregon Health Science University, Portland OR. July 2010.
• Conduct original research on the mechanism of drug resistance in the
emerging fungal pathogen Cryptoccocus gatti., including:
• Molecular characterization of putative ATP’s drug pumps.
• Construction of the knockout mutants for 3 ATP’s pumps.
• Understanding of the mechanism of Heteroresistance in C. gatti.
• Oversee all aspects of laboratory management, including at the project,
personnel and regulatory levels (e.g. IACUC, IRB, IBC, and laboratory
and biosafety)
Research Associate, Kumamoto Laboratory, Tufts University, Boston,
2003-2008
MA .
• Effect of antifungal uses in different Candida albicans growth
conditions.
• Sequencing and analysis of the clinical isolated C. albicans MDR1,
CDR1 promoter region’s
• Expression analysis of the MDR1 promoter region.
• RFLP studies of the clinical isolated C. albicans strains.
• Dissection of C. albicans biofilm formation process.
• Starting the studies of biofilm formation in C. Kumamoto
Laboratory
• Measurements of metabolic activities on C. albicans biofilm
formation
• Expression analysis on C. albicans biofilm formation
• Project level management and review of research staff, including
laboratory manager and research assistants.
2002 –2003
• Mentored college students on summer programs and students enroll on
other programs.
Igor Bruzual Ph.D.
2386 SW Vermont St. #29 Phone: 617-***-****
Portland Oregon 97219 Email:aboewc@r.postjobfree.com
2001-2002
Professional researcher, Laboratorio de Biología de Plásmidos (Plasmid
1997-2002 Biology Lab.) (IBE) (Experimental Biology Institute), Dr. Guillermina Alonso,.
Heavy Metal resistance in H plasmids.
Internship, Martin Luther University, Halle Germany, Dr. Dietrich Nies Group..
1996-1997
Heavy Metal resistance in H plasmids.
Graduate Student, Plasmid Biology Laboratory, IBE, Mentor Dr. Guillermina
Alonso. Heavy Metal resistance in H plasmids.
Research Technician. Consejo de Desarrollo Científico y Humanístico (CDCH).
Universidad Central de Venezuela. Prof. Raquel Pedroza. November 96 through
March 97.
Publications
Bruzual, I and C. Kumamoto (2011) Homozygosis for an MDR1 promoter
allele with higher promoter activity is common in clinically isolated strains of
Candida albicans. Mol Genet Genomics. 2011 Dec;286(5-6):347-57]
Bruzual, I; Riggle, P.; Hadley, S. and. Kumamoto, C.A. (2007), Biofilm
Formation by Fluconazole-Resistant Strains is Inhibited by Fluconazole, J
Antimicrob Chemother. Mar;59(3):441-50
Alonso, G; Vílchez, G.; Bruzual, I. and Rodríguez Lemoine (2001)
Characterization of plasmid MIP233 (IncHI3) of the H Complex. Research
Microbiology 153:149-153
Alonso, G.; Campos, Jenny; Bruzual A., Igor E. y Rodríquez-Lemoine, V.
(2000) Constrution of a cassette for cloning and analysis of replicons. Acta
Científica de Venezolana 51 (1)
Alonso, G.; Bruzual A., Igor E.; Campos, Jenny y Rodríquez-Lemoine,
V. (1999) Cloning and characterization of a replicon region the IncHII
plasmid pHH1457. FEMS Microbilogy Letters 179: 361 – 366.
Manuscripts in preparation
Basso. L., Gast C., Bruzual, I and Wong B. Characterization of novel
transmembrane proteins pumps involved on drug resistance on the emerging
fungal pathogen Cryptoccocus gatti. To be submitted to Plos Pathogens
Gast C., Basso. L., Bruzual, I and Wong B Azole resistance in Cryptococcus
gattii strains from the Pacific Northwest: lack of evidence for a role of
ERG11. To be submitted to J Antimicrob Chemother.
Bruzual, I and C. Kumamoto C. albicans MDR1 gene expression profile in
Bioflim forming conditions. In preparation for submission to Journal of
Antimicrobial and Chemotherapy
Igor Bruzual Ph.D.
2386 SW Vermont St. #29 Phone: 617-***-****
Portland Oregon 97219 Email:aboewc@r.postjobfree.com
Teaching Experience
Adjunct Professor General Molecular Genetics, Graduate program in
2001 Medical Science, School of Pharmacy. June 2001- August 2001
Adjunct Professor. General Molecular Genetics, Graduate program in
Pharmacology, School of Pharmacy. April 2001-June 2001
2001
Adjunct Professor, General Genetics Lab. School of Science, Central
University of Venezuela. October 1998-March 1999
Meetings and Congress
1998
Bruzual A., Igor E., Perry Riggle and Carol A. Kumamoto. Regulation of
Candida albicans MDR1 Gene Expression under Biofilm Conditions 108th
ASM General Meeting Boston, MA, June 1 -5, 2008.
2008 Igor Bruzual. "Candida albicans MDR1 drug resistance: The chicken or
the egg". Boston Yeast Area Meeting, BAYM., Harvard FAS Center for
Systems Biology Cambridge MA 02138. March 17th 2008
2008 Igor Bruzual. “Biofilm Formation in Candida albicans: Fluconazole
resistance is futile”. Boston Yeast Area Meeting, BAYM., White Head
Institute MIT. November 15th 2006
200 Bruzual A., Igor E., Perry Riggle, Susan Hadley and Carol A.
6 Kumamoto. Biofilm Formation by Fluconazole-Resistant Strains is
inhibited by Fluconazole 9th ASM Conference on Candida and
Candidiasis, Denver, Colorado, March 13 -17, 2006
2006
Bruzual A., Igor E., Susan Hadley and Carol A. Kumamoto Reversible
Activation Of The MDR1 Promoter In Wild Type Cells Following
Exposure To Fluconazole 8thASM Conference on Candida and
Candidiasis, Austin, Texas, March 18 -22, 2004
2004 Bruzual A., Igor E.; Alonso, G. y Rodríquez-Lemoine,V Caracterización
Preeliminar de la zona de replicación del Plásmido pHH1457 (IncHII)
(Characterization of the replication zone of pHH1457 plasmid)XIII.
Congreso de Bioquímica Clínica. March, 1997. Macuto Sheraton Hotel,
Macuto, Venezuela
199
7
Bruzual A., Igor E.; Alonso, G. y Rodríquez-Lemoine, V. Clonamiento
de los replicones de plásmidos del HI (Subgrupo HI3) e IncHII (Replicon
Cloning of Inc HI (subgoup HI3) and HII plasmid). XLV Convención
Anual de la AsoVAC. November, 1995. Universidad Simón Bolívar.
Caracas, Venezuela.
1995
Igor Bruzual Ph.D.
2386 SW Vermont St. #29 Phone: 617-***-****
Portland Oregon 97219 Email:aboewc@r.postjobfree.com
Courses
Microbiology diversity and biotechnology possibility in the rain forest.
200
Unión de Universidades Amazónicas, Universidad Central de Ve nezuela,
2
Amazonia Network y Comunidad Europea, November – December 2002
Bacterial Genetics, Theoretical and Practical Course, ICGEB Trieste Italy, 8-13
May, 2000
200
Genetics Patterns use in Forensic, Ethical and Moral Implications . (Uso de
0
Perfiles Genéticos en Forénsica y Paternidad, Implicaciones Éticas y Morales),
ONU- Biolac-Idea. Caracas, Venezuela. February 1-10, 2000.
200
0
Awards
Scholarship granted by Consejo Nacional de Investigaciones Científicas y
2001-2002
Tecnológicas (CONICIT) for short time research in Matin Luther University,
Halle Germany, December -april 2002
Scholarship granted by Deutcher Akademischer Austauschdienst (DAAD),
2001
German Government, for short time research in the Matin Luther University,
Halle Germany, July- December 2001
Scholarship granted by Consejo Nacional de Investigaciones Científicas
1997-2002 (Nationjal Council of National Research, CONICIT to complete the graduate
school