Medical Assistant

Peter Michael Broglie, Ph.D.

****-* ***** ***** ******* Rockville MD 20850

Telephone: 919-***-**** Email: abodvu@r.postjobfree.com

HIGHLIGHTS OF QUALIFICATIONS

• Strong analytical chemistry position ( GC/MS, GC/MS/MS, LC/MS, LC/MS/MS, LC/Q-ToF,

MALDI-TOF MS, IR, FT-IR, UV/Vis Spectroscopy, NMR)

• Highly trained in various types of cell culture and have experience with multiple cell lines ( primary

and immortalized keratinocytes and fibroblasts, A549, 293T, COS1, THP-1, Jurkat, U237, and

primary macrophages)

• Expert in protein analytical techniques ( Immunoblotting, Immunoprecipitation, Transient

Transfection, Stable Cell Transfection, Mass Spectroscopy Analysis, ELISA)

• Expert in post-translational modification analysis

• Have written protocols and journal articles on cell death mechanisms and detection of various forms

of cell death

• Highly Trained in molecular biology techniques ( Plasmid constructions, viral constructions, viral

infections, liposomal transfection, RT-PCR, PCR, flow cytometry )

• Strong background in microbiological techniques Familiar with animal handling techniques and

pathological analysis (H+E staining, immunohistochemistry, fluorescence microscopy, gross

pathology)

• Research experience in Government ( Washington DC OCME) Industrial (Bristol-Myers Squibb)

and Academic (NCSU and UNC) laboratories

• Familiar with many types of GC/MS and LC/MS automated systems ( AKTA©, Waters, Agilent) as

well as different types of HPLC chromatography methods ( Anion and Cation Exchange, Size

Exclusion, Chromatofocusing, Affinity, Reverse Phase)

• Strong leadership experience from serving as Vice President for Internal Affairs for the NCSU

Graduate Student Association, as well as President of Toxicology Graduate Student Association

• Strong public speaker due to experience teaching lectures and presenting research at conferences

• Works well in team settings through working with researchers from many different fields when a

member of STI Research Team at UNC Hospitals (e.g. social workers, physicians, epidemiologists)

EDUCATION

North Carolina State University, Ph.D. Molecular Toxicology

Thesis Title: The Role of TAK1 Associated Binding Protein 2 in Tumor Necrosis Factor Signaling and

Cell Death

Le Moyne College, B.S. Biochemistry

Thesis Title: Separation of Sialydated Species of a Novel Protein Using Chromatofocusing

AFFILIATIONS

• North Carolina Society of Toxicology (NCSOT)

• Society of Toxicology (SOT)

RELEVANT SKILLS

Academic:

• Chemistry: Advanced Biochemistry, Advanced Organic Chemistry, Analytical Chemistry,

Organic Chemistry, Physical Chemistry

• Biology: Advanced Molecular Biology, Microbiology, Statistics for Biological Sciences,

• Advanced Sciences: Biochemical Toxicology, Endocrinology, Pathology, Pharmacology,

Principles of Toxicology, Biochemical Toxicology, Pathology, Pathophysiology, Pharmacology,

Toxicology

Computer:

• Software: ImageJ, SPSS, SAS, IPLab, Photoshop, AlphaView, Vector NTI, Agilent and Waters

Chromatography Systems, Microsoft Office Suite

• Programming Languages: C/C++, Virtual C++, Visual Basic

• Operating Systems: MSDOS, Windows (Vista, 7), MAC OS

RESEARCH AND INDUSTRIAL EXPERIENCE

Forensic Toxicologist, Office of the Chief Medical Examiner, Washington DC, 2012-Present

• Responsible for accessioning and logging evidence from Medical Examiner, MPD, USCP,

USPP, USSS and other agencies

• Maintained and generated precise controls and internal standards for assays to determine

presence and quantitation of drugs of abuse as well as many pharmacological drugs

• Performed various drug screening assays (Acid/Neutral, Base, EIA)

• Performed drug quantitation on samples provided by Medical Examiner (for Post-Mortem

cases) or various police agencies (for DUI or drug-facilitated sexual assault cases)

• Responsible for maintaining inventory of more than 100 purified drug standards as well as

more than 50 deuterated internal standards

• Designed, conducted, and reported regulatory guideline toxicology studies in a Good

Laboratory Practice (GLP) environment

• Worked in method development group to generate new or update existing protocols to detect

drugs and/or chemicals in bodily fluids

• Evaluated toxicological data and prepared results for scientific journals

• Acted as Safety officer for OCME, inspecting lab and mortuary areas to ensure safe working

conditions for all employees and visitors

Post-Doctoral Fellow, Infectious Diseases, UNC School of Medicine, 2010-2012

• Determined the role of NLRP3 Post-Translational modifications on the activation and formation

of the inflammasome protein complex

• Generated mutant forms of NLRP3 to determine potential proteolytic sites (by either Caspase 1

or Cathepsin B) to observe the effects of proteolysis on NLRP3 activation

• Engineered over 60 plasmid constructs (truncation, mutant, viral and epitope tagged) to

overexpress for interaction and activation experiments

• Used cell-free systems to observe enzymatic cleavage of NLRP3 and through various

biochemical systems, was able to narrow down sites of activity on NLRP3

• Optimized protocol to reconstitute inflammasome and inflammatory signaling in 293T cells to

determine post-translational modifications

• Wrote protocols to transfect the THP-1 cell line as well as Bone Marrow Derived Mouse

macrophages with ~70-80% efficiency, two cell lines notoriously difficult to transfect short of

viral infection

• Analyzed various bacterial components in Neisseria and Staphylococcus for their effects on

inflammatory signaling and activation of various components in signaling pathways

• Determined the role of the inflammasome following bacterial pore-forming toxin exposure

• Determined the role of inflammasome signaling with lysosomal stability and mitochondrial

crosstalk

Research Assistant, Molecular Toxicology, North Carolina State University, 2005-2010

• Generated >30 fibroblast clones of TAB2 knockout cells used as a model to determine the

role of TAB2 in the TAK1 signaling pathway

• Determined the role of TAB2 not only included association with K63-linked polyubiquitin

chain but also this association aided in regulating phosphorylation status of TAK1

• Generated over 40 plasmid constructs containing either mutations, truncation or various

epitope tags to observe protein-protein interaction or activation

• Used inhibitor data as well as cell staining and electron microscopy protocols to determine

the exact means of cell death in TAB2 knockout cells

• Characterized relatively understudied form of cell death, necroptosis, and the role of the

TAK1 signaling axis in active necrotic cell death

Intern, Protein Purification, Bristol-Myers Squibb Company, Syracuse, NY, 2004-2005

• Wrote several protocols to use chromatofocusing technique to separate different sialydated

species of a monoclonal antibody in manufacture for therapeutic use

• Wrote protocols and algorithms for AKTA© system through UNICORN programming

software

• Developed automated programs for various HPLC systems (AKTA©, Waters, etc.) to run

multiple protocols at once and speed up production

• Determined mathematical algorithms for protein separation due to pH influences on both

anion and cation columns

TEACHING EXPERIENCE

Graduate Assistant, Toxicology, North Carolina State University, 2009-2010

• Led lectures along with advisor, Dr. Jun Ninomiya-Tsuji, in the role of the mitochondria during

toxin-induced cell death

• Wrote lectures for distance education course in Forensic Toxicology in Sample Preparation and

Metal Toxicity

Workshop Leader, Chemistry and Physics, Le Moyne College, 2003-2005

• Prepared questions for students taking Organic Chemistry, ran group sessions helping students

understand the basic concepts brought up in class

• Students always maintained high exam average and consistently received positive reviews from

students

PUBLICATIONS

• Holzinger D, Gieldon L, Mysore V, Nippe N, Taxman D, Duncan J, Broglie P, Marketon K,

Austermann J, Vogl T, Foell D, Niemann S, Peters G, Roth J, Löffler B, Staphylococcus aureus

Panton-Valentine Leukocidin Induces an Inflammatory Response in Human Phagocytes via the

NLRP3-Inflammasome, J. Leukocyte Bio, J Leukoc Biol. 2012 Nov;92(5):1069-81

• Taxman DJ, Swanson KV, Broglie PM, Wen H, Holley-Guthrie E, Huang MT, Callaway JB, Eitas

TK, Duncan JA, Ting JP., Porphyromonas gingivalis Mediates Inflammasome Repression in

Polymicrobial Cultures through a Novel Mechanism Involving Reduced Endocytosis., J Biol Chem.

2012 Sep 21;287(39):32791-9.

• Kebaier C, Chamberland RR, Allen IC, Gao X, Broglie PM, Hall JD, Jania C, Doerschuk CM, Tilley

SL, Duncan JA., Staphylococcus aureus α-Hemolysin Mediates Virulence in a Murine Model of

Severe Pneumonia Through Activation of the NLRP3 Inflammasome, J. Infect. Dis., J Infect Dis.

2012 Mar 1;205(5):807-17

• Morioka, S., Broglie, P., Takaesu, G., Matsumoto, K., and Ninomiya-Tsuji, J. TAK1 kinase and

TAK1 binding protein 2 regulate RIPK1-dependent apoptosis and necrosis. Submitted to Nature

Signaling

• Broglie, P., Matsumoto, K., Akira, S., Brautigan, D., Ninomiya-Tsuji, J. (2009). A TAK1 kinase

adaptor, TAB2, plays dual roles in TAK1 signaling by recruiting both an activator and an inhibitor of

TAK1 kinase in TNF signaling pathway, J. Biol. Chem. 285, 2333-2339 (2010)

• Prickett, T.D., Ninomiya-Tsuji, J., Broglie, P., Muratore, T.L., Shabanowitz, J., Hunt, D.F., and

Brautigan, D.L. (2008). TAB4 stimulates TAK1-TAB1 phosphorylation and binds polyubiquitin to

direct signaling to NF-κB. J. Biol. Chem.,192**-*****

PRESENTATIONS

• Broglie, P., Gao, X., Davis, B., Duncan J. A., Caspase 1 Mediated Cleavage of NLRP3, 2nd Annual

Lineberger Post-Doc Symposium, Chapel Hill, NC, October 2011

• Broglie, P., Inagaki, M., Matsumoto, K., Akira, S., Ninomiya-Tsuji, J. TAK1 Associated Binding

Protein 2 (TAB2) is Essential for NF-κB Activation in the TNF Signaling Pathway, Keystone

Symposia on NF-κB, Banff, Alberta, Canada, February 2008

• Broglie, P., Matsumoto, K., Akira, S., Ninomiya-Tsuji, J. The Role of TAB2 in the TNF Signaling

Pathway, NCSOT Regional Meeting, April 2007

HONORS AND AWARDS

• STD/HIV NIH Training Grant, Division of Infectious Diseases, UNC School of Medicine, 2010-

Present

• American Foundation for Aging Research, GSK Award, 03/2009

• Research Assistantship, NCSU Toxicology, 2007-2010

• Training Grant Award, NCSU Toxicology, 2005-2007

• Leadership Scholarship, Le Moyne College, 2001-2005

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