Medical Data Entry

Srikarthika Jambunathan

Email: abob66@r.postjobfree.com ? www.linkedin.com/in/srikarthikaj

Contact: +91-888-***-**** (Mobile)

Legal status: Citizen of USA

Career Objective

To gain a position in industry or academia, where I can contribute

with my skills in experimental design, organized work, trouble shoot

assays. Personal strengths include attention to detail, disciplined

efforts along with willingness to learn and being a team player.

Skills

> Biology: Mammalian cell and tissue culture. Stable cell line

development, transient transfections by electroporation or with

reagents and retrovirus, lentivirus, bacteria transformations.

Production, purification of lentivirus followed by infection of

cells with lentivirus. Culturing of different strains of E.coli

for various assays (protein expression, viral protein

expression)

> Molecular biology: Plasmid cloning, standard sub-cloning and

GATEWAY cloning, Vector design, creation, PCR, Real-Time PCR, RT-

PCR, RNA and mRNA extraction, Northern analysis, RNAi, shRNA

vector construction, Lentivirus infection of cells, Virus

production, SELEX assay to study protein-DNA interactions,

cryopreservation, DNA sequencing, Sequencing data analysis.

> Biochemistry: Protein extraction from mammalian cells to study

protein-protein interactions. Protein expression, isolation and

affinity purification of recombinant GST-, MBP- and His-tag

fusion proteins from different strains of E.coli, Silver

staining for protein detection. Tagged (FLAG-, myc-, HA-)

protein expression. SDS-PAGE, Western blots. Electromobility

gel shift assay (EMSA). Reporter (Luciferase) assay. In vitro

transcription and translation with rabbit reticulocyte and wheat

germ extract. Pulse-chase experiment, labeling nucleotides,

amino acids with radioisotopes 32P, 35S. Generating green

fluorescent (GFP) fusion proteins.

> Immunology: In situ immunofluorescence assay (IF),

immunocytochemistry (ICC), immunoprecipitation assay, co-

immunoprecipitation, chromatin immunoprecipitation assay (ChIP)

> In silico: Vector design and designing of primers. Amino acid

and nucleotide sequence analysis.

> Software tools: Seqscape sequencing data analysisExPASy, UCSC

Genome browser, BLAT, BLAST, Vector NTI. Adobe Photoshop, Word,

Excel, Powerpoint using both Macintosh and Windows platform,

Data management, Data entry

Professional Experience

Current status: Actively looking for a job that will complement with

my skills and experience in Cell and Molecular Biology. Open to a role

of Associate, Scientist /QC associate.

Boston University, Boston, MA

Research Associate 04/2010-

11/2011

Worked as a Research Associate at Boston University Medical Center.

Duties include lab management, organization, heading multiple

projects, ordering supplies and performing research experiments, data

analysis. Area of Research includes Diabetes and Obesity.

Accomplishments:

Constructed lentivirus-expressing vectors for infecting pre-adipocyte

cell lines (mouse and primary human cells).

Constructed shRNA lentiviral vectors for gene of interest. Generated a

stable cell line expressing shRNA against a gene of interest. Isolated

and purified virion particles and infected cells in culture.

University of Massachusetts Medical School, Worcester, MA

Postdoctoral Fellow

0 9/2007-01/2009

Conducted research on a novel zinc finger protein, NLZ1. Used SELEX

assay to determine the putative nucleotide sequence to which the NLZ1

zinc finger protein binds.

Accomplishments:

A stable neuronal cell line was developed that expresses a FLAG-NLZ1

fusion protein that will be used for ChIP assay followed by DEEP-

sequencing. A transgenic zebrafish line was generated that expresses

a myc-tagged NLZ1 fusion protein in order to study its role in

zebrafish hindbrain development.

Cleveland State University, Cleveland, OH

Research Assistant

2002-2006

Conducted research on the characterization of a novel ten-zinc fingers

protein ZXDC involved in Major Histocompatibility Complex class II

gene regulation.

Accomplishments:

Using mammalian cell culture, an ex vivo system, identified a post-

translational modification of the zinc finger protein, ZXDC as

SUMOylation.

Identified the single amino acid Lysine (659) of the ZXDC protein that

undergoes SUMOylation and demonstrated that SUMOylation enhances gene

transcriptional activity of the ZXDC protein. Also demonstrated that

SUMOylation does not interfere in protein-protein interactions between

the ZXDC protein and its binding partners.

Using the mammalian cell system showed that SUMOylation of the ZXDC

protein is important for transcriptional regulation of the Major

Histocompatibility Complex class II genes.

Education

Ph.D., Biological Sciences, 2006

Cleveland State University, Cleveland, OH

MS, Life Sciences, 1999

University of Mumbai, Mumbai, India

BS (Hons), Zoology, 1996

Sri Sathya Sai Institute of Higher Learning, AP, India

Publications

Jambunathan S, Yin J, Khan W, Puri V. 'FSP27 promotes lipid droplet

clustering and then fusion to regulate triglyceride accumulation',

2011 (Accepted, In Print, PLoS ONE)

Jambunathan S, and Joseph D Fontes, 'Sumoylation of ZXDC, a regulator

of MHC II transcription, affects the function of its transcriptional

activation domain.' Biol. Chem. 388, 965-72, 2007.

Al-Kandari W, Jambunathan S, Navalgund V, Koneni R, Freer M, Parimi N,

Mudhasani R, and Fontes JD. 'ZXDC, a novel zinc finger protein that

binds CIITA and activates MHC gene transcription.' Mol. Immunol.

44(4),311-21, 2007.

Al-Kandari W, Koneni R, Navalgund V, Aleksandrova A, Jambunathan S and

Joseph D. Fontes, 'The zinc finger proteins ZXDA and ZXDC form a

complex that binds CIITA and regulates MHC II gene transcription.' J.

Mol. Biol. 369(5),1175-87, 2007.

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